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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00960

High-density linkage map and mapping for sex and growth-related traits of largemouth bass (Micropterus salmoides)

 Chuanju Dong1, Peng Jiang2, Jiangfan Zhang1, xuejun Li1,  Li Shengjie2*, Junjie Bai2, Jianjia Fan2 and  Peng Xu3
  • 1Henan Normal University, China
  • 2Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, China
  • 3Xiamen University, China

Abstract

The largemouth bass is an important species, and its culture has risen sharply with the surge in fish aquaculture in China. Due to the lack of selective breeding technology for the largemouth bass, the growth rate and disease resistance are low, its sexual maturation is slow, and other serious problems are contributing to a sharp decline in the safety and quality of largemouth bass products in recent decades. Therefore, comprehensive breeding programs to improve the economic performance and promote the modern industrial development of largemouth bass must be considered a priority. Here, A total of 152 adult largemouth bass, including two parents and 150 progenies, were selected to produce the genetic mapping family. Then, a high-density linkage map was constructed based on restriction-site-associated DNA sequencing (RAD-Seq) using 6917 single nucleotide polymorphisms (SNPs) located in 24 linkage groups (LGs). The total genetic length of the linkage map was 1,261.96 cM, and the length of each LG varied from 24.72 cM for LG02 to 117.53 cM for LG16, with an average length of 52.58 cM and an average SNP number of 286. Thirteen significant quantitative trait loci (QTLs) for sex determination were located on LG04, LG05, LG08, LG12, LG15, LG21 and LG23. An informative QTL cluster that included 6 QTLs was detected on LG12. However, one notable QTL, which accounted for 71.48% of the total phenotypic variation, was located in the region of 1.85 cM on LG05. In addition, 32 identified QTLs were related to growth, including body weight (BW), body length (BL), body height (BH) and head length (HL). The QTLs for these growth-related traits are located in 13 LG regions and have little effect on phenotypic variation. This high-density genetic linkage map will enable the fine-mapping of economic traits and support the future genome assembly of the largemouth bass. Additionally, our study will be useful for future selective culture of largemouth bass and could potentially be used in molecular-assisted breeding of largemouth bass for aquaculture.

Keywords: Largemouth bass, linkage map, Single nucleotide polymorphism, sex determination, Growth-related, Quantitative Trait Loci

Received: 22 Apr 2019; Accepted: 09 Sep 2019.

Copyright: © 2019 Dong, Jiang, Zhang, Li, Shengjie, Bai, Fan and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Li Shengjie, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China, ssjjli@163.com