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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01000

The roles of four novel P450s genes in pesticides resistance in Apis cerana cerana Fabricius: Expression levels and detoxification efficiency

  • 1Shandong Agricultural University, China
  • 2Shandong Agricultural University, China

Abstract:
Cytochrome P450 monooxygenases (P450s) are widely distributed multifunctional enzymes that play crucial roles in insecticide detoxification or activation. In this study, to ascertain the molecular mechanisms of P450s in the detoxification of Chinese honeybees, Apis cerana cerana Fabricius (A. c. cerana), we isolated and characterized four new P450 genes (Acc301A1, Acc303A1, Acc306A1 and Acc315A1). The open reading frames of the four genes are 1263 to 1608 bp in length and encode four predicted polypeptides of 499 to 517 amino acids in length. Real-time quantitative PCR (RT-qPCR) results showed that expression of all four genes was observed in all developmental stages. Additionally, Western blot assays further indicated the real-time quantitative PCR results that showed that the four genes were induced by pesticide (thiamethoxam, deltamethrin, dichlorovos and paraquat) treatments. Furthermore, we also used dsRNA-mediated RNA interference to investigate the functions of Acc301A1, Acc303A1 and Acc306A1 in the antioxidant defense of honeybees. RNA interference targeting Acc301A1, Acc303A1, and Acc306A1 significantly increased the mortality rate of A. c. cerana upon pesticide treatment. These results provide important evidence about the role of the four P450 genes involved in detoxification.

Keywords: Cytochrome P450 monooxygenase, Apis cerana cerana Fabricius, RNA Interference, abiotic stresses, pesticides resistance

Received: 01 Mar 2019; Accepted: 20 Sep 2019.

Copyright: © 2019 Zhang, Yao, Wang, Liu, Ma, Wang and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Baohua Xu, Shandong Agricultural University, Tai'an, 271018, Shandong Province, China, bhxu@sdau.edu.cn