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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01097

Hypermethylation of NAT1 is a prognostic biomarker in colon adenocarcinoma

  • 1Affiliated Tumor Hospital of Guangxi Medical University, China
  • 2Liuzhou Traditional Chinese Medical Hospital Affiliated to Guangxi University of Chinese Medicine, China

d normal tissues. The correlation between mRNA and methylation of NAT1 were analyzed. Survival analysis was performed on CpG sites at the promoter region of NAT1. Association of mRNA and CpG sites of NAT1 with clinicopathological features was analyzed. A independent GEO dataset was used to validate the results.
Results: Expression of NAT1 mRNA was lower in COAD compared with normal tissues. The mean of eight CpG sites at the promoter region of NAT1 was higher in COAD compared with normal tissues. Five CpG sites were significantly negatively correlated to the NAT1 mRNA in COAD. Survival analysis showed that NAT1 mRNA and cg15797286, cg18509990 were associated with the overall survival (OS) of COAD patients. The jointed survival analysis revealed that combination of NAT1 mRNA with five CpG sites was significantly related to the OS of COAD patients. NAT1 mRNA and cg15797286 was associated with the T stage, N stage and clinical stage of COAD. Data from GSE128067 showed that cg15797286 was hypermethylation in colorectal adenomas with recurrence.
Conclusions: Methylation of NAT1 is associated with the development of COAD, and may be a prognostic and treatment markers for COAD.

Keywords: Methylation, Colon adenocarcinoma, prognosis, TCGA, N-Acetyltransferase 1

Received: 16 Jul 2019; Accepted: 11 Oct 2019.

Copyright: © 2019 Hu, Shi, Li, Long, Xie, Tang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Bangli Hu, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China, hubangli@gxmu.edu.cn