@ARTICLE{10.3389/fgene.2022.844385, AUTHOR={Chen, Yu and Zhang, Yongbai and Wang, Hongjie and Sun, Juan and Ma, Lichao and Miao, Fuhong and Zhang, Zixin and Cheng, Yang and Huang, Jianwei and Yang, Guofeng and Wang, Zengyu}, TITLE={A High-Quality Genome Assembly of Sorghum dochna}, JOURNAL={Frontiers in Genetics}, VOLUME={13}, YEAR={2022}, URL={https://www.frontiersin.org/articles/10.3389/fgene.2022.844385}, DOI={10.3389/fgene.2022.844385}, ISSN={1664-8021}, ABSTRACT={Sweet sorghum (Sorghum dochna) is a high-quality bio-energy crop that also serves as food for humans and animals. However, there is little information on the genomic characteristics of S. dochna. In this study, we presented a high-quality assembly of S. dochna with PacBio long reads, Illumina short reads, high-throughput chromosome capture technology (Hi-C) sequencing data, gene annotation, and a comparative genome analysis. The results showed that the genome of S. dochna was assembled to 777 Mb with a contig N50 of 553.47 kb and a scaffold N50 of 727.11 kb. In addition, the gene annotation predicted 37,971 genes and 39,937 transcripts in the genome of S. dochna. A Venn analysis revealed a set of 7,988 common gene annotations by integrating five databases. A Cafe software analysis showed that 191 gene families were significantly expanded, while 3,794 were significantly contracted in S. dochna. A GO enrichment analysis showed that the expanded gene families were primarily clustered in the metabolic process, DNA reconstruction, and DNA binding among others. The high-quality genome map constructed in this study provides a biological basis for the future analysis of the biological characteristics of S. dochna, which is crucial for its breeding.} }