ORIGINAL RESEARCH article

Front. Genet.
Sec. Livestock Genomics
doi: 10.3389/fgene.2022.898522

Genome-wide analysis identified candidate variants and genes associated with heat stress adaptation in Egyptian sheep breeds

 Adel M. Aboul Naga1*,  Alsamman M. Alsamman2*,  Achraf El Allali3, Mohmed H. Elshafie1, Ehab S. Abdelal1, Tarek M. Abdelkhalek1,  Taha H. Abdelsabour1, Lialy G. Mohmed1 and  Aladdin Hamwieh4
  • 1Animal Production Research Institute (APRI), Egypt
  • 2Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Egypt
  • 3African Genome Center, Mohammed VI Polytechnic University, Ben Guerir, Morocco, Morocco
  • 4International Center for Agriculture Research in the Dry Areas (ICARDA) Egypt, Egypt
Provisionally accepted:
The final, formatted version of the article will be published soon.

Heat stress caused by climatic changes is one of the most significant stresses on livestock in hot and dry areas. It has particularly adverse effects on the ability of the breed to maintain homeothermy. Developing countries are advised to protect and prepare their animal resources in the face of potential threats such as climate change. The current study was conducted in Egypt’s three hot and dry agro-ecological zones. Three local sheep breeds (Saidi, Wahati, and Barki) were studied with a total of 206 ewes. The animals were exercised under natural heat stress. The heat tolerance index of the animals was calculated to identify animals with high and low heat tolerance based on their response to meteorological and physiological parameters. Genomic variation in these breeds was assessed using 64,756 single nucleotide polymorphic markers (SNPs). From the perspective of comparative adaptability to harsh conditions, our objective was to investigate the genomic structure that might control the adaptability of local sheep breeds to environmental stress under hot and dry conditions. In addition, indices of population structure and diversity of local breeds were examined. Measures of genetic diversity showed a significant influence of breed and location on populations. The standardized index of association (rbarD) ranged from 0.0012 (Dakhla) to 0.026 (Assuit), while for the breed, they ranged from 0.004 (Wahati) to 0.0103 (Saidi). The index of association analysis (Ia) ranged from 1.42 (Dakhla) to 35.88 (Assuit) by location and from 6.58 (Wahati) to 15.36 (Saidi) by breed. The most significant SNPs associated with heat tolerance were found in the MYO5A, PRKG1, GSTCD, and RTN1 genes (P ≤ 0.0001). MYO5A produces a protein widely distributed in the melanin-producing neural crest of the skin. Genetic association between genetic and phenotypic variations showed that OAR118300122.1, located in ST3GAL3, had the greatest positive effect on heat tolerance. Genome-wide association analysis identified SNPs associated with heat tolerance in the PLCB1, STEAP3, KSR2, UNC13C , PEBP4, and GPAT2 genes.

Keywords: Sheep, Dry areas, Egypt, GWAS, SNP genotyping, heat tolerance

Received: 17 Mar 2022; Accepted: 05 Sep 2022.

Copyright: © 2022 Aboul Naga, Alsamman, El Allali, Elshafie, Abdelal, Abdelkhalek, Abdelsabour, Mohmed and Hamwieh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Adel M. Aboul Naga, Animal Production Research Institute (APRI), Giza, Egypt
Dr. Alsamman M. Alsamman, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, 12619, Giza, Egypt