Original Research ARTICLE
Transcription factor KLF10 constrains IL-17–committed Vγ4+ γδ T cells
- 1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, South Korea
- 2Academy of Immunology and Microbiology, Institute for Basic Science, South Korea
- 3College of Medicine, Inha University, South Korea
- 4Department of Animal Biotechnology, Chonbuk National University, South Korea
- 5Department of Oral Microbiology and Immunology, DRI and BK21 Plus Program, School of Dentistry, Seoul National University, South Korea
- 6Center for Food Bioconvergence, Seoul National University, South Korea
- 7Institute of Green Bio Science Technology, Seoul National University, South Korea
γδ T cells, known to be an important source of innate IL-17 in mice, provide critical contributions to host immune responses. Development and function of γδ T cells are directed by networks of diverse transcription factors. Here we examine the role of the zinc finger transcription factor, Kruppel-like factor 10 (KLF10), in the regulation of IL-17–committed CD27- γδ T (γδ27--17) cells. We found selective augmentation of Vγ4+ γδ27- cells with higher IL-17 production in KLF10-deficient mice. Surprisingly, KLF10-deficient CD127hi Vγ4+ γδ27--17 cells expressed higher levels of CD5 than their wild-type counterparts, with hyper-responsiveness to cytokine, but not T-cell receptor, stimuli. Thymic maturation of Vγ4+ γδ27- cells was enhanced in newborn mice deficient in KLF10. Finally, a mixed bone marrow chimera study indicated that intrinsic KLF10 signaling is requisite to limit Vγ4+ γδ27--17 cells. Collectively, these findings demonstrate that KLF10 regulates thymic development of Vγ4+ γδ27- cells and their peripheral homeostasis at steady state.
Keywords: KLF10, γδ T cells, IL-17, Homeostasis, transcrptional factor
Received: 04 Aug 2017;
Accepted: 23 Jan 2018.
Edited by:Wanjun Chen, National Institutes of Health (NIH), United States
Reviewed by:Vasileios Bekiaris, Technical University of Denmark, Denmark
Ashutosh Chaudhry, Memorial Sloan Kettering Cancer Center, United States
Copyright: © 2018 Kim, Gu, Kim, Ko, Kye, Kim, Cho, Lee, Song, Han and Yun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Cheol-Heui Yun, Seoul National University, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul, South Korea, firstname.lastname@example.org