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Front. Immunol. | doi: 10.3389/fimmu.2018.00334

Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

 Takuro Kobori1,  Shinichi Hamasaki2, Atsuhiro Kitaura2, Yui Yamazaki1, Takashi Nishinaka1, Atsuko Niwa1, Shinichi Nakao2,  Hidenori Wake3, Shuji Mori4, Tadashi Yoshino5, Masahiro Nishibori3 and  Hideo Takahashi1*
  • 1Department of Pharmacology, Faculty of Medicine, Kindai University, Japan
  • 2Department of Anesthesiology, Faculty of Medicine, Kindai University, Japan
  • 3Department of Pharmacology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Japan
  • 4School of Pharmacy, Shujitsu University, Japan
  • 5Department of Pathology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Japan

M2 macrophage promotes pathologic angiogenesis through a release of pro-angiogenic mediators or the direct cell-cell interaction with endothelium in the micromilieu of several chronic inflammatory diseases including rheumatoid arthritis and cancer where interleukin (IL)-18 also contributes to excessive angiogenesis. However, the detailed mechanism remains unclear. The aim of this study is to investigate the mechanism by which M2 macrophages in the micromilieu containing IL-18 induce excessive angiogenesis in the in vitro experimental model using mouse macrophage-like cell line, RAW264.7 cells, and mouse endothelial cell line, b.End5 cells. We discovered that IL-18 acts synergistically with IL-10 to amplify the production of macrophage-derived mediators like osteopontin and thrombin, yielding thrombin-cleaved form of osteopontin generation which acts through integrins α4/α9, thereby augmenting M2 polarization of macrophage with characteristics of increasing surface CD163 expression in association with morphological alteration. Furthermore, the results of visualizing temporal behavior and morphological alteration of macrophages during angiogenesis demonstrated that M2-like macrophages induced excessive angiogenesis through the direct cell-cell interaction with endothelial cells, possibly mediated by CD163.

Keywords: macrophage, CD163, Angiogenesis, Interleukin-18, Osteopontin, Thrombin

Received: 28 Nov 2017; Accepted: 06 Feb 2018.

Edited by:

Kottarappat N. Dileepan, Kansas University of Medical Center Research Institute, United States

Reviewed by:

Dong Li, Jilin University, China
Mieke Gouwy, KU Leuven, Belgium  

Copyright: © 2018 Kobori, Hamasaki, Kitaura, Yamazaki, Nishinaka, Niwa, Nakao, Wake, Mori, Yoshino, Nishibori and Takahashi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: MD, PhD. Hideo Takahashi, Faculty of Medicine, Kindai University, Department of Pharmacology, Osakasayama-shi, Osaka, Japan, hkt@med.kindai.ac.jp