Original Research ARTICLE
Early versus Late Admission to Labor Affects Labor Progression and Risk of Cesarean Section in Nulliparous Women
- 1Department for Epidemiology of Infectious Diseases, Hannover Medical School, Hannover, Germany
- 2Department of Epidemiology, Helmholtz-Centre for Infection Research, Braunschweig, Germany
- 3Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
- 4Epidemiology Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
- 5Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Naval Hospital Camp Lejeune, Camp Lejeune, NC, USA
- 6Midwifery Research and Education Unit, Department of Gynecology and Obstetrics, Hannover Medical School, Hannover, Germany
Background: Rates of cesarean section increase worldwide, and the components of this increase are partially unknown. A strong role is prescribed to dystocia, and at the same time, the diagnosis of dystocia is highly subjective. Previous studies indicated that risk of cesarean is higher when women are admitted to the hospital early in the labor.
Methods: We examined data on 1,202 nulliparous women with singleton, vertex pregnancies and spontaneous labor onset. We selected three groups based on cervical dilatation at admission: early (0.5–1.5 cm, N = 178), intermediate (2.5–3.5 cm, N = 320), and late (4.5–5.5 cm, N = 175). The Kaplan–Meier estimator was used to analyze the risk of delivery by cesarean section at a given dilatation, and thin-plate spline regression with a binary outcome (R library gam) to assess the form of the associations between the cesarean section in either the first or second stage versus vaginal delivery and dilatation at admission.
Results: Women who were admitted to labor early had a higher risk of delivery by cesarean section (18 versus 4% in the late admission group), while the risk of instrumental delivery did not differ (24 versus 24%). Before 4 cm dilatation, the earlier a woman was admitted to labor, the higher was her risk of delivery by cesarean section. After 4 cm dilatation, however, the relationship disappeared. These patterns were true for both first and second stage cesarean deliveries. Oxytocin use was associated with a higher risk of cesarean section only in the middle group (2.5–3.5 cm dilatation at admission).
Conclusion: Early admission to labor was associated with a significantly higher risk of delivery by cesarean section during the first and second stages. Differential effects of oxytocin augmentation depending on dilation at admission may suggest that admission at the early stage of labor is an indicator rather than a risk factor itself, but admission at the intermediate stage (2.5–3.5 cm) becomes a risk factor itself. Further research is needed to study this hypothesis.
The high and steadily increasing rates of cesarean section both in the US (1–4) and worldwide (5) are a cause for concern and provide motivation to study the underlying causes of these patterns. One potential explanation is the higher prevalence of medical interventions during labor, including a more liberal diagnosis of dystocia (6–10). Despite considerable efforts to define and standardize dystocia, there still remains substantial variety regarding the diagnosis of onset of labor, especially if it is taken into account that women usually diagnose labor onset by themselves (11, 12).
Meanwhile, several studies have determined that cesarean rates are higher among women admitted during the early stages of labor (i.e., with early cervical dilatation) than among those who were admitted at a later stage (i.e., with advanced cervical dilatation) (13–28). The reasons for this association are not clear. For example, the higher cesarean rates among women admitted during the early stages of labor could result from false labor and the subsequent pressure to expedite delivery (29, 30). In fact, early oxytocin augmentation is more likely among those admitted early (27, 31), and while it can hasten labor and prevent labor arrest in some cases (32), in others, it can lead to exhaustion and subsequent labor arrest (33, 34). Similarly, the early initiation of analgesia could make a longer labor more tolerable, but it can also be an indicator of underlying problems and might therefore be associated with increased probability of a cesarean section (35, 36). On the other side, early admission and the accumulation and cascade of following interventions itself can be an indicator of higher risk for abnormal labor (37–39).
In addition to observational studies, evidence is available from evaluations of triage programs that have been introduced to limit admission during the early stages of labor or reduce its effects (13, 17, 27, 40). The studies generally demonstrated only minor, respective non-significant effects of interventions, but could not explain this observation.
So far, the mechanism by which early admission increases the risk of cesarean section remains unclear, providing necessity for further studies. While there is consensus that risk of cesarean is elevated in women with early admission, the previous studies did not assess this association in a longitudinal fashion during the labor progression and in detail allowing addressing potential non-linearity in the expected effects. For example, whether the higher risk is only related to first stage cesarean section or whether it is accumulated in early stages of labor, indicating overall poor progress of labor. Therefore, we aimed at assessing the association between cervical dilatation at admission and labor progression as well as the risk of cesarean section during the first and second stages of labor and its interplay with oxytocin augmentation.
Materials and Methods
The analysis employs data collected for a previous study assessing the impact of an increase in the rates of epidural analgesia and the risk of cesarean section, the details of which are published elsewhere (41). In brief, two samples of 500–700 women each were selected from two time periods – one prior to an introduction of the policy advocating the use of epidural analgesia for all women during labor in a military hospital in Hawaii (1992/1993) and the other after the introduction (1995/1996). The study was limited to nulliparous women between 18 and 34 years of age at admission, with singleton, vertex pregnancies where the gestational age, based on the last menstrual period or ultrasound, was estimated to be between 37 0/7 and 41 6/7 weeks at delivery. These women had experienced a spontaneous onset of labor and were able to give birth to infants that weighed between 2,500 and 4,000 g. A woman was ineligible for the study if either her dilatation upon admission was >7 cm or labor duration from admission until birth was <3 h, as there would potentially be insufficient time for the administration of an epidural analgesia. In total, 1,329 women were recruited for the original study; however, for the purposes of our analysis, we used data from 1,202 women for whom records of dilatation at admission were available.
Trained midwives reviewed the medical records of the study participants to obtain information on demographic characteristics, assessment of cervical dilatation at admission, labor progression, and administration of any analgesia, birth, and postpartum circumstances.
This study was approved by the Institutional Review Boards of the National Institute of Child Health and Human Development, National Institutes of Health, and the Tripler Army Medical Center. As the analysis was based on medical records only, no informed consent was required.
First, we tabulated the maternal characteristics and variables related to labor for the whole sample and for three groups based on the extent of dilatation at admission: (1) 0.5–1.5 cm, (2) 2.5–3.5 cm, and (3) 4.5–5.5 cm. These three non-adjacent ranges were selected, so as to avoid classification problems at cut-off values. The first two groups fall within the latent phase of labor, whereas the third is classified as the active phase, based on the criterion of more than 4 cm for the active phase which was valid at the time when the research was performed (42). Differences across groups were tested by Wilcoxon and chi-square test. Second, we assessed the relationship between the extent of dilatation at admission and the risk of cesarean section during the first and second stages employing thin-plate spline regression (R library gam). This permitted the inspection of the form of the association (43). In this analysis, the total sample was included. Subsequently, we used the Kaplan–Meier life table analysis to study the patterns of oxytocin augmentation and cesarean section over the course of labor in the three subgroups defined by dilatation at admission. Vaginal birth was considered as the end of the studied period, and labor progress was expressed in terms of dilation. In the analysis of the oxytocin augmentation, first stage cesarean was considered as a censoring event. Differences between groups were tested by log-rank test. Finally, we studied the probability cesarean section in the subgroups of dilatation at admission comparing women who received oxytocin and those who did not.
The distribution of dilatations at admission for the 1,202 participants who were included in the analysis is presented in Figure 1. About half (53%) of the women were admitted with dilatations below 4 cm. There was clear rounding tendency, with dilatation much more often ending in full centimeter than in 0.5 cm. This rounding tendency supports our decision to analyze subgroups based on non-adjacent dilatation ranges in order to minimize the misclassification error. No differences in maternal age, BMI, and birth weight were observed across the three subgroups of dilatation at admission (Table 1). The oxytocin augmentation and cesarean sections were significantly more frequent among women with <3.5 cm dilatation at admission, as compared with among those with dilatations between 4.5 and 5.5 cm. The risk of instrumental vaginal delivery did not vary across the three groups.
The risk of first and second stages cesarean section decreased approximately linearly with the dilatation at admission for dilatations below 5 cm and was constant for dilatations above 5 cm (Figure 2). The data for women with >5 cm dilatation, however, were sparse, and the results may therefore be unreliable, as indicated by the wide confidence bands (Figure 2).
Figure 2. Probability of cesarean section during the first and second stages of labor for women with a different dilatation at admission. Note: analysis for second stage excludes women who had cesarean in the first stage. Dotted lines indicate pointwise 95% confidence intervals for the first stage cesarean delivery.
In all three dilatation subgroups, the risk of cesarean section was constant during labor (as measured by dilation), with higher rates in the early admission groups (Figure 3). As a consequence, among women who were admitted early (i.e., with dilatations of <4 cm) and where a cesarean section before reaching 5 cm dilatation was not conducted, the risk of cesarean at more advanced dilatation was still slightly elevated, as compared with those who were admitted with a dilatation of more than 4 cm (p = 0.013 in log-rank test).
Figure 3. Kaplan–Meier survival curves for cesarean delivery (left) and initiation of oxytocin augmentation (right) by cervical dilatation. Note: model for augmentation considers women who were delivered by cesarean before augmentation as censored. Second stage is displayed as dilatation of 11 cm.
By contrast, labor augmentation with oxytocin followed a different pattern. Among most women admitted with <4 cm dilatation, the use of oxytocin was initiated shortly (measured by dilation) after admission (Figure 3, right panel). However, if a woman passed the 5-cm dilation mark without receiving oxytocin, the initiation of oxytocin use did not vary significantly across the three dilatation subgroups (p = 0.09 in log-rank test).
In the total sample, oxytocin use was associated with a higher risk of cesarean section (Table 2). In the subgroups defined on the basis of cervical dilatation at admission, there was no association in the groups admitted with 0.5–1.5 cm dilatation and with 4.5–5.5 cm dilatation (in the latter group possibly due to a too small sample). In contrast, there was a strong effect in the middle group (2.5–3.5 cm dilatation at admission).
Table 2. Mode of birth depending on the administration of oxytocin among women admitted with different cervical dilatations.
Our results show that the probability of cesarean section was much higher for women admitted early as compared with those admitted late, which is consistent with several previous studies (13–26). We could show that the elevated risk persisted during both the active phase and the second stage of labor and that it was not accumulated during a specific time of labor. Oxytocin augmentation was more frequent among women admitted early, and in most cases, the oxytocin augmentation was initiated shortly after admission (in terms of dilation). For the observed higher risk of cesarean section among women admitted to labor early, there are two potential explanations: first, the risk is pre-existent and early admission is an indicator of problems during the course of labor and, second, the risk increase occurs during the course of labor, with early admission facilitating a cascade of interventions finally resulting in cesarean section (37, 44). For example, some women admitted early may receive interventions like oxytocin augmentation because of the expectation that they should progress in the labor. They may not respond to the labor stimulation. Since these women may not be in true labor, early admission could be resulting in the diagnosis of dystocia and performance of a cesarean section (9, 17). The third possibility is that mixture of both mechanisms become active in the same woman and also that in some women the one mechanism is responsible, while in the others the other mechanism.
The ascertained results allow rejection of some hypotheses regarding the course of labor in women after early admission (for example, we showed that in case of early admission, the risk of cesarean section is not concentrated in the early labor, and that the risk of cesarean is elevated through the first and second stages). A possible mechanism for such concentration of risk in the early labor could be the failure to account for the non-linearity in the dilation process. The risk of cesarean section may be higher when predictions regarding dilation in the early stages of labor are based on what is expected for dilation at a later stage of labor given the different transition times and based on the action lines when a partogram is used (42, 45–47). This would suggest problems in management of labor. Yet, our findings of constant cesarean probabilities across all stages of dilation do not support this hypothesis.
Our finding of frequent oxytocin administration shortly after admission in the early admission group could suggest that labor progress in these women is hampered from the beginning and continue to be such during the complete course of labor (37, 38). However, the early use of oxytocin can also contribute to the observed elevated risk for cesarean section: women who receive oxytocin early in labor may be exhausted by the time they reach the second stage, resulting in the diagnosis of dystocia at this late stage (33). Interesting observation is that oxytocin administration was associated with no change in risk of cesarean among women in earliest and latest studied admission groups, while it was in the intermediate admission group. This suggests a non-linear effect of oxytocin on the mode of birth in the process of labor. Non-linear effects of interventions have been observed regarding labor duration and mode of birth only recently (48). Assuming that oxytocin augmentation is one of the potential elements of the cascade of interventions during labor, the interpretation could be that women in the earliest admission group are those bringing their elevated risk into labor, whereas in the intermediate admission group, there is a contribution of the management of labor to the increased risk. The mechanism in this intermediate admission group might be explained by uterine hyperstimulation, resulting in fetal acidosis as an indication for cesarean section (34, 49). A review analyzing partogram action lines found the lowest incidence of oxytocin augmentation and cesarean sections with a 4-h action line and excluding the latent phase (47). If the women are admitted at a later stage of labor, the oxytocin effects are less pronounced (or at least matter less, because cesarean section is generally less common). Unfortunately, our data did not contain information on the indication for oxytocin augmentation. Further studies are necessary to assess the potentially differential effects of oxytocin administration depending on the dilatation at admission and its association with labor progress. Early administration of oxytocin alone or in combination with amniotomy was shown to shorten labor duration (34). At the same time, reviews found inconsistent results regarding the prevention of cesarean section (32, 34).
Fortunately, taking advantage of data collected during the change in the policy of conducting epidural analgesia, we were able to indirectly control the effects of differential pain perception. For example, a woman may perceive the early stages of labor as painful, and the resulting anxiety could lead to an early admission in the hospital which nowadays is in some places provided with special offers for early labor care (15, 50). A previous study found higher rates and an earlier initiation of epidural analgesia when women perceived their personal onset of labor earlier than their midwife diagnosed the onset of labor (51). Therefore, a low individual pain threshold and a high anxiety level, together with a difficult labor, may result in higher rates of cesarean section (44). In the analyzed data, epidural analgesia that typically can be applied to relieve pain was determined mainly by policy and not by individual preferences/needs of the women.
Strengths and Limitations
The strength of our study is the homogenous database of nulliparous women and the in-depth analysis of the risk of cesarean during the progress of the labor. Furthermore, as stated above, taking advantage of data collected during a “natural experiment” of epidural analgesia, we were able to leave this variable out from the consideration. The main limitation is the observational nature of this study; since direct causal inference is not possible, we were only able to generate hypotheses for further research, as in relation to oxytocin. Another limitation is the measurement error and subjectivity inherent in measurement of cervical dilation.
Early admission to labor was associated with a significantly higher risk of cesarean section, which depended on dilatation at admission. This finding is consistent with previous literature and supplements the previous knowledge by demonstrating a constant risk during the labor progress and a risk elevation for first and second stages of labor. The fact that oxytocin administration was associated with the risk of cesarean section depending on dilatation at admission may suggest that admission at the early stage of labor (0.5–1.5 cm) is an indicator rather than a risk factor itself, but it becomes a risk factor when admissions occur in the intermediate stage (2.5–3.5 cm). Future research should address this specific hypothesis in more detailed analyses.
RM performed the analysis and drafted the manuscript; JZ conceptualized the research question, performed the original study, and provided comments on the manuscript; JG, LC, AP, and MG contributed to interpretation of the data and the writing of the manuscript. All authors approved the version being submitted.
The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the United States Government.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This research was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development.
1. Declercq E, Menacker F, MacDorman M. Rise in “no indicated risk” primary caesareans in the United States, 1991-2001: cross sectional analysis. BMJ (2005) 330(7482):71–2. doi:10.1136/bmj.38279.705336.0B
4. Ford J, Grewal J, Mikolajczyk R, Meikle S, Zhang J. Primary cesarean delivery among parous women in the United States, 1990-2003. Obstet Gynecol (2008) 112(6):1235–41. doi:10.1097/AOG.0b013e31818ce092
5. Villar J, Valladares E, Wojdyla D, Zavaleta N, Carroli G, Velazco A, et al. Caesarean delivery rates and pregnancy outcomes: the 2005 WHO global survey on maternal and perinatal health in Latin America. Lancet (2006) 367(9525):1819–29. doi:10.1016/S0140-6736(06)68704-7
8. Kjaergaard H, Olsen J, Ottesen B, Nyberg P, Dykes AK. Obstetric risk indicators for labour dystocia in nulliparous women: a multi-centre cohort study. BMC Pregnancy Childbirth (2008) 8:45. doi:10.1186/1471-2393-8-45
9. Kjaergaard H, Olsen J, Ottesen B, Dykes AK. Incidence and outcomes of dystocia in the active phase of labor in term nulliparous women with spontaneous labor onset. Acta Obstet Gynecol Scand (2009) 88(4):402–7. doi:10.1080/00016340902811001
10. Kjaergaard H, Dykes AK, Ottesen B, Olsen J. Risk indicators for dystocia in low-risk nulliparous women: a study on lifestyle and anthropometrical factors. J Obstet Gynaecol (2010) 30(1):25–9. doi:10.3109/01443610903276417
11. Gross MM, Burian RA, Fromke C, Hecker H, Schippert C, Hillemanns P. Onset of labour: women’s experiences and midwives’ assessments in relation to first stage duration. Arch Gynecol Obstet (2009) 280(6):899–905. doi:10.1007/s00404-009-0990-7
12. Eri TS, Blystad A, Gjengedal E, Blaaka G. Negotiating credibility: first-time mothers’ experiences of contact with the labour ward before hospitalisation. Midwifery (2010) 26(6):e25–30. doi:10.1016/j.midw.2008.11.005
16. Klein MC, Kelly A, Kaczorowski J, Grzybowski S. The effect of family physician timing of maternal admission on procedures in labour and maternal and infant morbidity. J Obstet Gynaecol Can (2004) 26(7):641–5. doi:10.1016/S1701-2163(16)30611-9
17. Janssen PA, Still DK, Klein MC, Singer J, Carty EA, Liston RM, et al. Early labor assessment and support at home versus telephone triage: a randomized controlled trial. Obstet Gynecol (2006) 108(6):1463–9. doi:10.1097/01.AOG.0000247644.64154.bb
19. Holmes P, Oppenheimer LW, Wen SW. The relationship between cervical dilatation at initial presentation in labour and subsequent intervention. BJOG (2001) 108(11):1120–4. doi:10.1016/S0306-5456(01)00265-0
20. Klein M, Lloyd I, Redman C, Bull M, Turnbull AC. A comparison of low-risk pregnant women booked for delivery in two systems of care: shared-care (consultant) and integrated general practice unit. I. Obstetrical procedures and neonatal outcome. Br J Obstet Gynaecol (1983) 90(2):118–22. doi:10.1111/j.1471-0528.1983.tb08894.x
21. Klein M, Lloyd I, Redman C, Bull M, Turnbull AC. A comparison of low-risk pregnant women booked for delivery in two systems of care: shared-care (consultant) and integrated general practice unit. II. Labour and delivery management and neonatal outcome. Br J Obstet Gynaecol (1983) 90(2):123–8. doi:10.1111/j.1471-0528.1983.tb08894.x
23. Bailit JL, Dierker L, Blanchard MH, Mercer BM. Outcomes of women presenting in active versus latent phase of spontaneous labor. Obstet Gynecol (2005) 105(1):77–9. doi:10.1097/01.AOG.0000147843.12196.00
24. Impey L, Hobson J, O’Herlihy C. Graphic analysis of actively managed labor: prospective computation of labor progress in 500 consecutive nulliparous women in spontaneous labor at term. Am J Obstet Gynecol (2000) 183(2):438–43. doi:10.1067/mob.2000.105899
25. Roshanfekr D, Blakemore KJ, Lee J, Hueppchen NA, Witter FR. Station at onset of active labor in nulliparous patients and risk of cesarean delivery. Obstet Gynecol (1999) 93(3):329–31. doi:10.1016/S0029-7844(98)00451-7
28. Kauffman E, Souter VL, Katon JG, Sitcov K. Cervical dilation on admission in term spontaneous labor and maternal and newborn outcomes. Obstet Gynecol (2016) 127(3):481–8. doi:10.1097/AOG.0000000000001294
31. Neal JL, Lamp JM, Buck JS, Lowe NK, Gillespie SL, Ryan SL. Outcomes of nulliparous women with spontaneous labor onset admitted to hospitals in preactive versus active labor. J Midwifery Womens Health (2014) 59(1):28–34. doi:10.1111/jmwh.12160
32. Wei S, Wo BL, Qi HP, Xu H, Luo ZC, Roy C, et al. Early amniotomy and early oxytocin for prevention of, or therapy for, delay in first stage spontaneous labour compared with routine care. Cochrane Database Syst Rev (2013) 8:CD006794. doi:10.1002/14651858.CD006794.pub4
34. Bugg GJ, Siddiqui F, Thornton JG. Oxytocin versus no treatment or delayed treatment for slow progress in the first stage of spontaneous labour. Cochrane Database Syst Rev (2013) 6:CD007123. doi:10.1002/14651858.CD007123.pub3
35. Wong CA, Scavone BM, Peaceman AM, McCarthy RJ, Sullivan JT, Diaz NT, et al. The risk of cesarean delivery with neuraxial analgesia given early versus late in labor. N Engl J Med (2005) 352(7):655–65. doi:10.1056/NEJMoa042573
37. Petersen A, Poetter U, Michelsen C, Gross MM. The sequence of intrapartum interventions: a descriptive approach to the cascade of interventions. Arch Gynecol Obstet (2013) 288(2):245–54. doi:10.1007/s00404-013-2737-8
38. Tracy SK, Sullivan E, Wang YA, Black D, Tracy M. Birth outcomes associated with interventions in labour amongst low risk women: a population-based study. Women Birth (2007) 20(2):41–8. doi:10.1016/j.wombi.2007.03.005
39. Petersen A, Ayerle GM, Fromke C, Hecker H, Gross MM; ProGeb Study Team. The timing of interventions during labour: descriptive results of a longitudinal study. Midwifery (2011) 27(6):e267–73. doi:10.1016/j.midw.2010.10.017
40. Hodnett ED, Stremler R, Willan AR, Weston JA, Lowe NK, Simpson KR, et al. Effect on birth outcomes of a formalised approach to care in hospital labour assessment units: international, randomised controlled trial. BMJ (2008) 337:a1021. doi:10.1136/bmj.a1021
41. Zhang J, Yancey MK, Klebanoff MA, Schwarz J, Schweitzer D. Does epidural analgesia prolong labor and increase risk of cesarean delivery? A natural experiment. Am J Obstet Gynecol (2001) 185(1):128–34. doi:10.1067/mob.2001.113874
42. Spong CY, Berghella V, Wenstrom KD, Mercer BM, Saade GR. Preventing the first cesarean delivery: summary of a joint Eunice Kennedy Shriver National Institute of Child Health and Human Development, Society for Maternal-Fetal Medicine, and American College of Obstetricians and Gynecologists Workshop. Obstet Gynecol (2012) 120(5):1181–93. doi:10.1097/AOG.0b013e3182704880
44. Haines HM, Rubertsson C, Pallant JF, Hildingsson I. The influence of women’s fear, attitudes and beliefs of childbirth on mode and experience of birth. BMC Pregnancy Childbirth (2012) 12:55. doi:10.1186/1471-2393-12-55
46. Zhang J, Landy HJ, Branch DW, Burkman R, Haberman S, Gregory KD, et al. Contemporary patterns of spontaneous labor with normal neonatal outcomes. Obstet Gynecol (2010) 116(6):1281–7. doi:10.1097/AOG.0b013e3181fdef6e
47. Lavender T, Hart A, Smyth RM. Effect of partogram use on outcomes for women in spontaneous labour at term (review). Cochrane Database Syst Rev (2012) 8:CD005461. doi:10.1002/14651858.CD005461.pub3
48. Gross MM, Fromke C, Hecker H. The timing of amniotomy, oxytocin and neuraxial analgesia and its association with labour duration and mode of birth. Arch Gynecol Obstet (2014) 289(1):41–8. doi:10.1007/s00404-013-2916-7
Keywords: cesarean section, Kaplan-Meier life table analysis, oxytocin, dilation, early admission, timing of interventions
Citation: Mikolajczyk RT, Zhang J, Grewal J, Chan LC, Petersen A and Gross MM (2016) Early versus Late Admission to Labor Affects Labor Progression and Risk of Cesarean Section in Nulliparous Women. Front. Med. 3:26. doi: 10.3389/fmed.2016.00026
Received: 24 April 2016; Accepted: 21 May 2016;
Published: 27 June 2016
Edited by:Issam Lebbi, Ob-Gyn and Fertility Private Clinic, Dream Center, Tunisia
Reviewed by:Süleyman Cansun Demir, Çukurova University, Turkey
Zaleha Abdullah Mahdy, National University of Malaysia, Malaysia
Copyright: © 2016 Mikolajczyk, Zhang, Grewal, Chan, Petersen and Gross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rafael T. Mikolajczyk, firstname.lastname@example.org