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Front. Med., 19 June 2019 | https://doi.org/10.3389/fmed.2019.00129

Immunomodulatory Role of Capsular Polysaccharides Constituents of Cryptococcus neoformans

  • 1Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil
  • 2Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
  • 3Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
  • 4Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

Cryptococcosis is a systemic fungal infection caused by Cryptococcus neoformans. In immunocompetent patients, cryptococcal infection is often confined to the lungs. In immunocompromised individuals, C. neoformans may cause life-threatening illness, either from novel exposure or through reactivation of a previously acquired latent infection. For example, cryptococcal meningitis is a severe clinical disease that can manifest in people that are immunocompromised due to AIDS. The major constituents of the Cryptococcus polysaccharide capsule, glucuronoxylomannan (GXM), and galactoxylomannan (GalXM), also known as glucuronoxylomanogalactan (GXMGal), are considered the primary virulence factors of Cryptococcus. Despite the predominance of GXM in the polysaccharide capsule, GalXM has more robust immunomodulatory effects on host cellular immunity. This review summarizes current knowledge regarding host-Crytococcus neoformans interactions and the role of capsular polysaccharides in host immunomodulation. Future studies will likely facilitate a better understanding of the mechanisms involved in antigenic recognition and host immune response to C. neoformans and lead to the development of new therapeutic pathways for cryptococcal infection.

Introduction

In Cryptococcus neoformans, capsular polysaccharides are located externally to the cell wall. The polysaccharides constituting the capsule can be found attached to the cell wall or released into the environment, in which case they are termed exopolysaccharides. Biochemical studies have shown that the C. neoformans capsule primarily comprises glucuronoxylomannan (GXM), representing ~88% of the capsule. GXM is a polymer that consists mostly of a linear α-(1–3)-mannan substituted with β-(1–2)-glucopyranosyluronic acid and β-(1–4)-xylopyranosyl. The C. neoformans capsule also comprises 10% galactoxylomannan (GalXM), also called glucuronoxylomannogalactan (GXMGal) and 2% mannoproteins. The GalXM consists of an α-(1 → 6)-galactan backbone with galactomannan side chains that are further substituted with variable numbers of xylose and glucuronic acid residues (1). It has recently been characterized that the polysaccharide capsule can function as a flotation device to facilitate transport and dispersion in aqueous fluids (2).

It is known that, the manifestation and severity of cryptococcal infections, as well as the clinical presentation of the disease depend on the immune response of the patient (3, 4). Clinical and experimental studies have suggested that cryptococcosis is controlled by cell-mediated immunity in immunocompetent patients who develop a Th1 response (5, 6). In addition, the production of inflammatory mediators induces the death of C. neoformans potentiating the antimicrobial activity of immune cells (712).

The Involvement of the cellular-mediated immune response in the cryptococcosis has been described. Macrophages and dendritic cells are related in the recognition, phagocytosis, and presentation of antigens and activation of the host response (13, 14). The involvement of the NK cells has been characterized in response to the C. neoformans; after the recognition via β-integrins, cytolytic degranulation occurs leading to the death of fungal cells in a manner similar to that observed in tumor cells (15, 16). Despite the importance of the immune response in the C. neoformans infection, it has recently been described that the sudden increase of this response could cause non-specific symptoms favoring tissue damage; this phenomenon is known as inflammatory response syndrome (IRIS) (1721). In some severe cases, medications such as corticosteroids are required to suppress inflammatory response until resolution of infection (22, 23).

Innate Response to C. neoformans

Initial infection of C. neoformans occurs via inhalation of infectious propagules and subsequent colonization of the respiratory tract. Macrophages and dendritic cells phagocytize the opsonized fungi. C. neoformans and their secreted products can be recognized by many cellular receptors, including the mannose receptors CD14, TLR2, TLR4, and CD18 (13, 2427). The TLR-associated adapter protein, MyD88, is known to play an important role in immune response to C. neoformans, as mice deficient in MyD88 have increased susceptibility to Cryptococcosis (2729). Macrophages are important for defense against Cryptococcus and respond to C. neoformans infection by releasing pro-inflammatory signals such as the chemokines IL-1α, and IL-1β. Furthermore, mice that do not encode the IL1R1 receptor, the expression of which is stimulated through MyD88, exhibit more disease phenotypes when compared to the wild type mice (30, 31).

In addition to phagocytosis, the killing of C. neoformans is crucial for successful host response. The internalization of viable forms may facilitate the dissemination of this microorganism to different parts of the vertebrate host. Macrophages become efficient effector cells when stimulated by pro-inflammatory cytokines, increasing microbicidal mechanisms, including lysosomal fusion, acidophagy of phagosomes, iron sequestration, and enzymatic degradation of proteins. Extracellular killing of the yeast is mediated by the release of cytokines, NO, and ROS (3234). C. neoformans can also be sequestered in tissues by multinucleated giant cells (35). Moreover, it has recently been demonstrated that extracellular vesicles of C. neoformans can stimulate macrophages, which increases its antimicrobial activity and induces direct death of the fungus (36).

Similar to bacteria, fungi have developed strategies to survive within phagocytes. Most of these pathogens kill the host cell to escape and infect other tissues (37, 38). It has recently been suggested that C. neoformans can exploit monocytes and macrophages by using these cells as “Trojan horses,” allowing them to disseminate throughout the host without being detected by the immune system (3840). Tucker and Casadevall have shown that opsonized C. neoformans cells are capable of being internalized in <2 min by alveolar macrophages or J774 lineage (41). However, infected macrophages are unable to maintain the acidity of the phagolysosome, which results in phagolysosome membrane lysis, C. neoformans replication, concomitant release of the polysaccharides, and eventual lysis of infected macrophages. Complementing Tucker and Casadevall's work, a later study observed lysis of alveolar epithelial cells 18 h after infection with C. neoformans (42). Subsequently, it was demonstrated that C. neoformans can also escape from macrophages through a mechanism that does not result in cell death (43). After the onset of intracellular replication, C. neoformans are able to extrude from the macrophage phagosome into extracellular space, an event that has been termed “phagosome extrusion,” Phagosome extrusion involves the formation of large fungal cell-filled phagosomes followed by phagosome ejection and subsequent survival and further replication of the fungal cells in the macrophage extracellular space (44). This data suggests that non-lytic expulsion of C. neoformans is a phenomenon that may have significant clinical implications in understanding the invasion of the central nervous system by cells that serve as “Trojan horses” for C. neoformans (40, 4547).

The development of cell-mediated protective immunity against C. neoformans depends, in part, on the activation of dendritic cells by the fungus (48). Dendritic cells are also important for antifungal immunity, as they can recognize and phagocyte different fungi, such as C. neoformans, Aspergillus fumigatus, Candida albicans, and Histoplasma capsulatum (4953). Mannose and FcγRII receptors present on dendritic cells are essential for C. neoformans phagocytose and antigen presentation to T cells (54). After dendritic cells identify and phagocytize C. neoformans, they express costimulatory molecules, migrate to the lymphoid tissues, and secrete cytokines (53, 55, 56). After real-time imaging, Wozniak and Levitz demonstrated that C. neoformans can be found in lysosomal compartments 20 min after the onset of phagocytosis, resulting in the degradation of the fungal cells followed by antigen presentation to T cells (57).

The development of cell-mediated protective immunity against C. neoformans depends, in part, on the activation of dendritic cells by the fungus (48). Dendritic cells also represent an important interface between innate and adaptive immunity (5860). In addition, the interaction between dendritic cells and C. neoformans modulates T cell responses in vitro (6163). In vivo observations have determined that T cells migrate to the thoracic lymph nodes after infection (6466). In addition, both dendritic cells and CD4+ T cells were detected in bronchoalveolar infiltrate from lungs of C. neoformans infected mice. This phenomenon coincided with increased expression of IL-12 cytokines from both subunits (p40 and p35) and the presence of IFN-γ in the lung homogenate (67).

Adaptive Response to C. neoformans

C. neoformans infection is normally controlled by cell-mediated immunity in immunocompetent patients who develop a Th1 response following the production of IL-12, TNF-α, IFN-γ, and NO (5, 10, 6870). CD4+T cells producing IFN-γ (71) are important for inducing immune defenses that control the growth and propagation of C. neoformans (70, 72). The formation of granulomas also serves as an important antifungal mechanism (73, 74). In addition to CD4+ T cells, granulomas also contain macrophages, NK cells, and CD8+ T cells (73). Effector NK cells and CD8+ T cells are potent producers of perforin and granulysin that destroy cells infected with C. neoformans and directly destroy the fungus itself (75, 76). Recent studies with the cryptococcosis murine model have demonstrated that Th1 and Th17 subtype responses are associated with disease protection and Th2 responses are deleterious for the host (7779).

Immunomodulatory Role of Capsular Constituents

Capsules are commonly found in bacteria. However, the only pathogenic encapsulated fungus described so far is Cryptococcus. In this model, the capsule is the most studied structure since it is considered one of the main virulence factors. When in the saprophytic phase, the polysaccharide capsule protects the fungus against various types of stress and dehydration. In the murine experimental model, acapsular mutants do not cause cryptococcosis or have reduced virulence. Other studies have demonstrated that the capsular polysaccharides GXM and GalXM, have immunomodulatory properties and improve fungal survival by aiding host immune evasion (80). In addition, the fungus releases constituents of the polysaccharide capsule which can easily be found in body fluids during infection (81). These capsular constituents exert various immunomodulatory roles in host cells and are been used as research models by several research groups.

Immunomodulatory Activity of GXM

C. neoformans is considered an opportunistic intracellular pathogen (82) and, as mentioned previously, can survive, and even replicate inside macrophages. The role of the capsule in intracellular parasitism was demonstrated mostly using the CAP67 mutant, which does not contain GXM in the capsule and is not able to replicate within phagocytic cells (83). This indicates that the capsule, particularly GXM, plays a key role in intracellular parasitism. GXM is also involved in the inhibition of phagocytosis probably due to the fact that this molecule is polyanionic, which may cause an electrostatic repulsion that prevents host immune cells from interacting with and eliminating fungal cells (84). Inhibition of phagocytic events will also decrease the amount of presented antigen to T cell and, consequently, reduces host immune response (85). A GXM molecule could also present distinct combinations of motifs, demonstrating the complex heteropolymeric structure of this capsular polysaccharide. Recently, the presence of a secreted lactonohydrolase (LHC1) of C. neoformans, has been characterized in the formation of a higher order capsular structure proposing enzymatic remodeling after assembly of polysaccharides in the extracellular space and thus inhibiting the deposition of components of the complement system, thus avoiding the destruction of the fungus by formation of the membrane attack complex and the formation of fragments iC3b, which are important opsonic (86).

Several research groups have suggested that GXM behaves as a molecule that contributes to immune suppression. Syme et al. (54) demonstrated that GXM is directly involved with FcRγII expression in monocytes, macrophages, and dendritic cells, which results in an inhibitory signal that suppresses host immune response. GXM also induces secretion of IL-10 and IL-8 in human monocytes and neutrophils (87, 88). IL-10 is a potent suppressor of proinflammatory cytokines, which suggests that C. neoformans-induced IL-10 secretion interferes with cell-mediated immunity (89). In 2001, GXM was reported to indirectly reduce IL-12 secretion in monocytes by directly increasing IL-10 secretion in monocytes co-cultured with T cells (71). GXM also induces the accumulation of TGF-β in macrophages (90). In contrast to mice infected with a hypocapsular mutant, mice infected with encapsulated strains were not able to produce Th1 cytokines, due to the accumulation of significant amounts of IL-10. These results suggest that yeasts encapsulated with GXM are able to inhibit the development of a Th1-type protective response via the induction of IL-10 (71, 91). Despite this, the immunosuppressive activity of GXM has beneficial effects in other types of inflammatory pathologies. GXM can act as a potent immunosuppressant during induced arthritis (92) and inhibits NETs production in neutrophils (93).

In addition to having potent immunosuppressive effects on cytokine production, GXM is also capable of inducing apoptosis in different systems. Monari et al. (94) have demonstrated that Fas-L expression in macrophages induces apoptosis of activated T lymphocytes. In 2008, the same group demonstrated that apoptosis of T lymphocytes involves intrinsic and extrinsic pathways (95). Furthermore, our group observed that capsular GXM is able to induce apoptosis in macrophages through a mechanism that involves the increased production of Fas and Fas-L (90). This ability of GXM expands the repertoire of immunosuppressive activities observed during cryptococcosis pathogenesis and complements other reports in literature, where Fas-induced apoptosis has been described in infections caused by other microorganisms (73, 96). The mechanism of GXM-induced cell death may also be related to inhibition of glycolytic flux (97).

Chiapello et al. (98) demonstrated that GXM is capable of inducing cellular apoptosis in different organs after treatment. Apoptosis was also observed in vitro in rat splenocytes (99). The authors suggested that apoptosis would be a consequence of an immunomodulation of the host defense during infection. GXM deposition in tissue, increased splenocyte production and release of the anti-inflammatory cytokines may lead to these processes (98100). In rats infected with C. neoformans, T cell production of IL-10 could reduce LFA-1 expression and IL-2 production, resulting in decreased T-cell activation and proliferation capacity. Chiapello et al. (98) also demonstrated that total splenocytes from infected mice and activated by concanavalin A (ConA), increased IL-10 production. Addition of anti-IL-10 antibody reversed the suppressor effect, suggesting that this cytokine would be related to suppress total splenocyte proliferation (100). Thus, the production of IL-10 during cryptococcosis could prevent the activation of T cells-mediated control of C. neoformans, resulting in the spread of the disease. These observations confirm other studies that have demonstrated that pathogen-derived molecules promote apoptosis in the host cell, and thus they may be important factors in the survival of infectious microorganisms (101, 102).

Immunomodulatory Activity of GalXM

The majority of studies on the immunomodulatory effects of capsular polysaccharide from C. neoformans has been performed with GXM. There are fewer studies demonstrating the immunomodulatory functions of GalXM, but they have increased in recent years. Chaka et al. (103) demonstrated that GalXM is capable of inducing the production of TNF-α in peripheral blood mononuclear cells (PBMC). In addition, our group has observed that GalXM induces TNF-α release and iNOS expression, which results in NO production (90).

Delfino et al. (104) showed that GalXM induces IL-6 release by monocytes. In 2006, another group demonstrated that prolonged stimulation with the CAP67 mutant increases expression of IL-6 in PBMC and results in resolution of infection, whereas that does not occur encapsulated fungus (105). This suggests that GXM and GalXM have different immunomodulatory activities. Despite this, GalXM is also capable of inducing apoptosis in different cells of the immune response. Pericolini et al. (106) demonstrated that GalXM induces apoptosis in human T cells, at a rate ~50 times greater than GXM, via activation of caspase 8. This suggests that GalXM suppresses the proliferation of T cells. Our group also demonstrated that GalXM induces apoptosis in macrophages mediated by Fas/FasL interaction. This effect was greater than that observed by GXM (90). GalXM-mediated cell death could enhance the suppressive effect of GXM during cryptococcosis.

Galactose, a major component of GalXM, has been described as an important virulence factor of C. neoformans (107). The enzymes UDP-Glc epimerase and UDP-Gal transporter participate in GalXM biosynthesis and are present in GXM positive and GalXM negative phenotypes. Moyrand et al. demonstrated that strains encoding mutated forms of the enzymes UDP-Glc epimerase (uge1Δ) and UDP-Gal transporter (ugt1Δ) are unable to colonize the brain in the first days of infection. These results suggest that although these constituent polysaccharides have different profiles, they may act together to suppress the host's immune response during infection. Recently, it was demonstrated that treatment with GalXM activates dendritic cells and induces Th17 differentiation when co-cultured with T cells. The authors also demonstrated that treating mice with GalXM prior to infection induced immune protection and this phenomenon was dependent on IL-6 and IL-17 production (108).

The Importance of Xylosylation

UDP-Xyl is the substrate for the synthesis of xylose-containing glycoconjugates (109). In C. neoformans xylose is present in the capsular constituents GXM and GalXM and in yeast membrane glycoinositolphosphorylceramides (GIPCs) (110, 111). The enzyme β-1,2-xylosyltransferase transfers xylose to α-1,3-dimannoside to form the sequence Xyl-β-1,2-α-1,3-Man. In a cxt1 mutant strain of C. neoformans, which is deficient in beta-xylosyltransferase, shows decrease or lack of xylose units in GalXM and GXM, respectively. This mutante strain presents low virulence in the murine model (111). Mutant strains that encode a deletion in the UDP-xyl synthase gene (uxs1) produce GXM without β(1,2)-xylose (112). The genes CAS31, CAS32, CAS33, CAS34, and CAS35 are homologous to CAS3 and help regulate the addition of xylose units onto the GXM side chain (113). Experiments using the Δuxs1 strain, which does not encode UDP-Xyl synthase, suggest that the presence of xylose units in the GXM structure contributes to C. neoformans virulence by delaying the deposition of the C3 complement system factor and altering the accumulation of GXM in the spleen (114). Moyrand et al. (112) observed that a cohort of mice infected with the Δuxs1 strain survived after 60 days of infection, whereas mice infected with the wild type strain died 30 days post-infection. Animals infected with Δuxs1 strain were sacrificed 90 days after infection and no C. neoformans colonies were observed in mouse brains. These results suggest the importance of xylosylation for C. neoformans virulence and infectivity. In addition, deletion of the Cxt1 gene, which is responsible for encoding a β(1,2)-xylosyltransferase involved in capsule synthesis, led to lower levels of infection by C. neoformans in murine models of disease (115). These data suggests that xylose is important for basal interactions between the pathogen and the host (116).

Conclusions

C. neoformans is an opportunistic fungus that has a polysaccharide capsule that exhibits important immunomodulatory activity in vivo. Both GXM and GalXM polysaccharides exert modulatory effects on different components of the innate and adaptive immune response. The function of all cell types is impacted by capsular polysaccharides, including macrophages which are important phagocytes and antigen-presenting cells that reside in various tissues throughout the body. The C. neoformans polysaccharides are important pathogen associated molecular patterns that are essential for stimulating cell-mediated immunity. However, there is no cell receptor that specifically recognizes these molecules. The cell-host recognition may occur through different receptors present on the cellular surface.

Further understanding the interactions between capsular polysaccharides and immune cells as well as the immunomodulatory effects of capsular polysaccharides may lead to the development of vaccines against this important pathogen.

Author Contributions

DD-R, IL-d-F, JR, DN, MN, AM, LF-d-L, JP, LM-P, and CF-d-L: wrote the paper. All authors read and approved the final version of the manuscript.

Funding

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

1. McFadden DC, Fries BC, Wang F, Casadevall A. Capsule structural heterogeneity and antigenic variation in Cryptococcus neoformans. Eukaryot Cell. (2007) 6:1464–73. doi: 10.1128/EC.00162-07

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Vij R, Cordero RJB, Casadevall A. The Buoyancy of Cryptococcus neoformans is affected by capsule size. mSphere. (2018) 3:e00534-18. doi: 10.1128/mSphere.00534-18

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Romani L, Kaufmann SH. Immunity to fungi: editorial overview. Res Immunol. (1998) 149:277–281. doi: 10.1016/S0923-2494(98)80751-7

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Romani L. Immunity to fungal infections. Nat Rev Immunol. (2004) 4:1–23. doi: 10.1038/nri1255

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Huffnagle GB, Yates JL, Lipscomb MF. Immunity to a pulmonary Cryptococcus neoformans infection requires both CD4+ and CD8+ T cells. J Exp Med. (1991) 173:793–800. doi: 10.1084/jem.173.4.793

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Buchanan KL, Doyle HA. Requirement for CD4(+) T lymphocytes in host resistance against Cryptococcus neoformans in the central nervous system of immunized mice. Infect Immun. (2000) 68:456–62. doi: 10.1128/IAI.68.2.456-462.2000

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Kawakami K, Qifeng X, Tohyama M, Qureshi MH, Saito A. Contribution of tumour necrosis factor-alpha (TNF-alpha) in host defence mechanism against Cryptococcus neoformans. Clin Exp Immunol. (1996) 106:468–74. doi: 10.1046/j.1365-2249.1996.d01-870.x

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Kawakami K, Tohyama M, Teruya K, Kudeken N, Xie Q, Saito A. Contribution of interferon-gamma in protecting mice during pulmonary and disseminated infection with Cryptococcus neoformans. FEMS Immunol Med Microbiol. (1996) 13:123–30. doi: 10.1111/j.1574-695X.1996.tb00225.x

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Kawakami K, Tohyama M, Xie Q, Saito A. IL-12 protects mice against pulmonary and disseminated infection caused by Cryptococcus neoformans. Clin Exp Immunol. (1996) 104:208–14. doi: 10.1046/j.1365-2249.1996.14723.x

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Herring AC, Lee J, McDonald RA, Toews GB, Huffnagle GB. Induction of interleukin-12 and gamma interferon requires tumor necrosis factor alpha for protective T1-cell-mediated immunity to pulmonary Cryptococcus neoformans infection. Infect Immun. (2002) 70:2959–64. doi: 10.1128/IAI.70.6.2959-2964.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Koguchi Y, Kawakami K. Cryptococcal infection and Th1-Th2 cytokine balance. Int Rev Immunol. (2002) 21:423–38. doi: 10.1080/08830180213274

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Zhou Q, Gault RA, Kozel TR, Murphy WJ. Protection from direct cerebral cryptococcus infection by interferon-gamma-dependent activation of microglial cells. J Immunol. (2007) 178:5753–61. doi: 10.4049/jimmunol.178.9.5753

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Shoham S, Huang C, Chen JM, Golenbock DT, Levitz SM. Toll-like receptor 4 mediates intracellular signaling without TNF-alpha release in response to Cryptococcus neoformans polysaccharide capsule. J Immunol. (2001) 166:4620–6. doi: 10.4049/jimmunol.166.7.4620

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Albuquerque PC, Fonseca FL, Dutra FF, Bozza MT, Frases S, Casadevall A, et al. Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. Future Microbiol. (2014) 9:147–61. doi: 10.2217/fmb.13.163

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Li SS, Ogbomo H, Mansour MK, Xiang RF, Szabo L, Munro F, et al. Identification of the fungal ligand triggering cytotoxic PRR-mediated NK cell killing of Cryptococcus and Candida. Nat Commun. (2018) 9:751. doi: 10.1038/s41467-018-03014-4

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Xiang RF, Li S, Ogbomo H, Stack D, Mody CH. beta1 integrins are required to mediate NK cell killing of Cryptococcus neoformans. J Immunol. (2018) 201:2369–76. doi: 10.4049/jimmunol.1701805

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Wiesner DL, Boulware DR. Cryptococcus-related immune reconstitution inflammatory syndrome(IRIS): pathogenesis and its clinical implications. Curr Fungal Infect Rep. (2011) 5:252–61. doi: 10.1007/s12281-011-0064-8

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Eschke M, Piehler D, Schulze B, Richter T, Grahnert A, Protschka M, et al. A novel experimental model of Cryptococcus neoformans-related immune reconstitution inflammatory syndrome (IRIS) provides insights into pathogenesis. Eur J Immunol. (2015) 45:3339–50. doi: 10.1002/eji.201545689

PubMed Abstract | CrossRef Full Text | Google Scholar

19. Heung LJ. Innate immune responses to Cryptococcus. J Fungi. (2017). 3:35. doi: 10.3390/jof3030035

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Campuzano A, Wormley FL. Innate immunity against Cryptococcus, from recognition to elimination. J Fungi. (2018). 4:33. doi: 10.3390/jof4010033

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Shourian M, Qureshi ST. Resistance and tolerance to cryptococcal infection: an intricate balance that controls the development of disease. Front Immunol. (2019) 10:66. doi: 10.3389/fimmu.2019.00066

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Neal LM, Xing E, Xu J, Kolbe JL, Osterholzer JJ, Segal BM, et al. CD4(+) T cells orchestrate lethal immune pathology despite fungal clearance during Cryptococcus neoformans meningoencephalitis. MBio. (2017) 8:e01415-17. doi: 10.1128/mBio.01415-17

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Mehta GU, Panackal AA, Murayi R, Bennett JE, Williamson PR, Chittiboina P. Corticosteroids for shunted previously healthy patients with non-HIV cryptococcal meningoencephalitis. J Neurol Neurosurg Psychiatry. (2018) 89:219–20. doi: 10.1136/jnnp-2017-315830

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Mansour MK, Schlesinger LS, Levitz SM. Optimal T cell responses to Cryptococcus neoformans mannoprotein are dependent on recognition of conjugated carbohydrates by mannose receptors. J Immunol. (2002) 168:2872–9. doi: 10.4049/jimmunol.168.6.2872

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Fonseca FL, Nohara LL, Cordero RJ, Frases S, Casadevall A, Almeida IC, et al. Immunomodulatory effects of serotype B glucuronoxylomannan from Cryptococcus gattii correlate with polysaccharide diameter. Infect Immun. (2010) 78:3861–70. doi: 10.1128/IAI.00111-10

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Piccioni M, Monari C, Bevilacqua S, Perito S, Bistoni F, Kozel TR, et al. A critical role for FcgammaRIIB in up-regulation of Fas ligand induced by a microbial polysaccharide. Clin Exp Immunol. (2011) 165:190–201. doi: 10.1111/j.1365-2249.2011.04415.x

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Sato K, Kawakami K. Recognition of Cryptococcus neoformans by pattern recognition receptors and its role in host defense to this infection. Med Mycol J. (2017) 58:J83–90. doi: 10.3314/mmj.17.011

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Yauch LE, Mansour MK, Shoham S, Rottman JB, Levitz SM. Involvement of CD14, toll-like receptors 2 and 4, and MyD88 in the host response to the fungal pathogen Cryptococcus neoformans in vivo. Infect Immun. (2004) 72:5373–82. doi: 10.1128/IAI.72.9.5373-5382.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Wang JP, Lee CK, Akalin A, Finberg RW, Levitz SM. Contributions of the MyD88-dependent receptors IL-18R, IL-1R, and TLR9 to host defenses following pulmonary challenge with Cryptococcus neoformans. PLoS ONE. (2011) 6:e26232. doi: 10.1371/journal.pone.0026232

PubMed Abstract | CrossRef Full Text | Google Scholar

30. Vecchiarelli A, Dottorini M, Pietrella D, Monari C, Retini C, Todisco T, et al. Role of human alveolar macrophages as antigen-presenting cells in Cryptococcus neoformans infection. Am J Respir Cell Mol Biol. (1994) 11:130–7. doi: 10.1165/ajrcmb.11.2.8049074

PubMed Abstract | CrossRef Full Text | Google Scholar

31. Shourian M, Ralph B, Angers I, Sheppard DC, Qureshi ST. Contribution of IL-1RI signaling to protection against Cryptococcus neoformans 52D in a mouse model of infection. Front Immunol. (2017) 8:1987. doi: 10.3389/fimmu.2017.01987

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Flesch IE, Schwamberger G, Kaufmann SH. Fungicidal activity of IFN-gamma-activated macrophages. Extracellular killing of Cryptococcus neoformans. J Immunol. (1989) 142:3219–24.

PubMed Abstract | Google Scholar

33. Lovchik JA, Lyons CR, Lipscomb MF. A role for gamma interferon-induced nitric oxide in pulmonary clearance of Cryptococcus neoformans. Am J Respir Cell Mol Biol. (1995) 13:116–24. doi: 10.1165/ajrcmb.13.1.7598935

PubMed Abstract | CrossRef Full Text | Google Scholar

34. Shoham S, Levitz SM. The immune response to fungal infections. Br J Haematol. (2005) 129:569–82. doi: 10.1111/j.1365-2141.2005.05397.x

PubMed Abstract | CrossRef Full Text | Google Scholar

35. Okubo Y, Wakayama M, Ohno H, Yamamoto S, Tochigi N, Tanabe K, et al. Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans. Jpn J Infect Dis. (2013) 66:216–21. doi: 10.7883/yoken.66.216

PubMed Abstract | CrossRef Full Text | Google Scholar

36. Oliveira DL, Freire-de-Lima CG, Nosanchuk JD, Casadevall A, Rodrigues ML, Nimrichter L. Extracellular vesicles from Cryptococcus neoformans modulate macrophage functions. Infect Immun. (2010) 78:1601–9. doi: 10.1128/IAI.01171-09

PubMed Abstract | CrossRef Full Text | Google Scholar

37. Nguyen L, Pieters J. The Trojan horse: survival tactics of pathogenic mycobacteria in macrophages. Trends Cell Biol. (2005) 15:269–76. doi: 10.1016/j.tcb.2005.03.009

PubMed Abstract | CrossRef Full Text | Google Scholar

38. Denham ST, Brown JCS. Mechanisms of pulmonary escape and dissemination by Cryptococcus neoformans. J Fungi. (2018) 4:E25. doi: 10.3390/jof4010025

PubMed Abstract | CrossRef Full Text | Google Scholar

39. Sorrell TC, Juillard PG, Djordjevic JT, Kaufman-Francis K, Dietmann A, Milonig A, et al. Cryptococcal transmigration across a model brain blood-barrier: evidence of the Trojan horse mechanism and differences between Cryptococcus neoformans var. grubii strain H99 and Cryptococcus gattii strain R265. Microb Infect. (2016) 18:57–67. doi: 10.1016/j.micinf.2015.08.017

PubMed Abstract | CrossRef Full Text | Google Scholar

40. Santiago-Tirado FH, Onken MD, Cooper JA, Klein RS, Doering TL. Trojan horse transit contributes to blood-brain barrier crossing of a eukaryotic pathogen. MBio. (2017) 8:e02183-16. doi: 10.1128/mBio.02183-16

PubMed Abstract | CrossRef Full Text | Google Scholar

41. Tucker SC, Casadevall A. Replication of Cryptococcus neoformans in macrophages is accompanied by phagosomal permeabilization and accumulation of vesicles containing polysaccharide in the cytoplasm. Proc Natl Acad Sci USA. (2002) 99:3165–70. doi: 10.1073/pnas.052702799

PubMed Abstract | CrossRef Full Text | Google Scholar

42. Barbosa FM, Fonseca FL, Holandino C, Alviano CS, Nimrichter L, Rodrigues ML. Glucuronoxylomannan-mediated interaction of Cryptococcus neoformans with human alveolar cells results in fungal internalization and host cell damage. Microb Infect. (2006) 8:493–502. doi: 10.1016/j.micinf.2005.07.027

PubMed Abstract | CrossRef Full Text | Google Scholar

43. Ma H, Croudace JE, Lammas DA, May RC. Expulsion of live pathogenic yeast by macrophages. Curr Biol. (2006) 16:2156–60. doi: 10.1016/j.cub.2006.09.032

PubMed Abstract | CrossRef Full Text | Google Scholar

44. Alvarez M, Casadevall A. Phagosome extrusion and host-cell survival after Cryptococcus neoformans phagocytosis by macrophages. Curr Biol. (2006) 16:2161–5. doi: 10.1016/j.cub.2006.09.061

PubMed Abstract | CrossRef Full Text | Google Scholar

45. Charlier C, Nielsen K, Daou S, Brigitte M, Chretien F, Dromer F. Evidence of a role for monocytes in dissemination and brain invasion by Cryptococcus neoformans. Infect Immun. (2009) 77:120–7. doi: 10.1128/IAI.01065-08

PubMed Abstract | CrossRef Full Text | Google Scholar

46. Casadevall A. Cryptococci at the brain gate: break and enter or use a Trojan horse? J Clin Invest. (2010) 120:1389–92. doi: 10.1172/JCI42949

CrossRef Full Text | Google Scholar

47. Tseng HK, Huang TY, Wu AY, Chen HH, Liu CP, Jong A. How Cryptococcus interacts with the blood-brain barrier. Future Microbiol. (2015) 10:1669–82. doi: 10.2217/fmb.15.83

PubMed Abstract | CrossRef Full Text | Google Scholar

48. Bauman SK, Nichols KL, Murphy JW. Dendritic cells in the induction of protective and nonprotective anticryptococcal cell-mediated immune responses. J Immunol. (2000) 165:158–67. doi: 10.4049/jimmunol.165.1.158

PubMed Abstract | CrossRef Full Text | Google Scholar

49. Johnston SA, May RC. The human fungal pathogen Cryptococcus neoformans escapes macrophages by a phagosome emptying mechanism that is inhibited by Arp2/3 complex-mediated actin polymerisation. PLoS Pathog. (2010) 6:e1001041. doi: 10.1371/journal.ppat.1001041

PubMed Abstract | CrossRef Full Text | Google Scholar

50. Zheng NX, Wang Y, Hu DD, Yan L, Jiang YY. The role of pattern recognition receptors in the innate recognition of Candida albicans. Virulence. (2015) 6:347–61. doi: 10.1080/21505594.2015.1014270

PubMed Abstract | CrossRef Full Text | Google Scholar

51. Maldonado S, Fitzgerald-Bocarsly P. Antifungal activity of plasmacytoid dendritic cells and the impact of chronic HIV infection. Front Immunol. (2017) 8:1705. doi: 10.3389/fimmu.2017.01705

PubMed Abstract | CrossRef Full Text | Google Scholar

52. Ray SC, Rappleye CA. Flying under the radar: Histoplasma capsulatum avoidance of innate immune recognition (2018). Semin Cell Dev Biol. 89:91–8. doi: 10.1016/j.semcdb.2018.03.009

PubMed Abstract | CrossRef Full Text | Google Scholar

53. Wozniak KL. Interactions of Cryptococcus with dendritic cells. J Fungi. (2018) 4:E36. doi: 10.3390/jof4010036

PubMed Abstract | CrossRef Full Text | Google Scholar

54. Syme RM, Spurrell JC, Amankwah EK, Green FH, Mody CH. Primary dendritic cells phagocytose Cryptococcus neoformans via mannose receptors and Fcgamma receptor II for presentation to T lymphocytes. Infect Immun. (2002) 70:5972–81. doi: 10.1128/IAI.70.11.5972-5981.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

55. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. (1998) 392:245–52. doi: 10.1038/32588

PubMed Abstract | CrossRef Full Text | Google Scholar

56. Eastman AJ, Osterholzer JJ, Olszewski MA. Role of dendritic cell-pathogen interactions in the immune response to pulmonary cryptococcal infection. Future Microbiol. (2015) 10:1837–57. doi: 10.2217/fmb.15.92

PubMed Abstract | CrossRef Full Text | Google Scholar

57. Wozniak KL, Levitz SM. Cryptococcus neoformans enters the endolysosomal pathway of dendritic cells and is killed by lysosomal components. Infect Immun. (2008) 76:4764–71. doi: 10.1128/IAI.00660-08

PubMed Abstract | CrossRef Full Text | Google Scholar

58. Palucka K, Banchereau J. Dendritic cells: a link between innate and adaptive immunity. J Clin Immunol. (1999) 19:12–25. doi: 10.1023/A:1020558317162

PubMed Abstract | CrossRef Full Text | Google Scholar

59. Janeway CA Jr, Medzhitov R. Innate immune recognition. Annu Rev Immunol. (2002) 20:197–216. doi: 10.1146/annurev.immunol.20.083001.084359

PubMed Abstract | CrossRef Full Text | Google Scholar

60. Shimizu K, Fujii S. An adjuvant role of in situ dendritic cells (DCs) in linking innate and adaptive immunity. Front Biosci. (2008) 13:6193–201. doi: 10.2741/3147

PubMed Abstract | CrossRef Full Text | Google Scholar

61. Herring AC, Falkowski NR, Chen GH, McDonald RA, Toews GB, Huffnagle GB. Transient neutralization of tumor necrosis factor alpha can produce a chronic fungal infection in an immunocompetent host: potential role of immature dendritic cells. Infect Immun. (2005) 73:39–49. doi: 10.1128/IAI.73.1.39-49.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

62. Pietrella D, Corbucci C, Perito S, Bistoni G, Vecchiarelli A. Mannoproteins from Cryptococcus neoformans promote dendritic cell maturation and activation. Infect Immun. (2005) 73:820–7. doi: 10.1128/IAI.73.2.820-827.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

63. Dan JM, Wang JP, Lee CK, Levitz SM. Cooperative stimulation of dendritic cells by Cryptococcus neoformans mannoproteins and CpG oligodeoxynucleotides. PLoS ONE. (2008) 3:e2046. doi: 10.1371/journal.pone.0002046

PubMed Abstract | CrossRef Full Text | Google Scholar

64. Traynor TR, Kuziel WA, Toews GB, Huffnagle GB. CCR2 expression determines T1 versus T2 polarization during pulmonary Cryptococcus neoformans infection. J Immunol. (2000) 164:2021–7. doi: 10.4049/jimmunol.164.4.2021

PubMed Abstract | CrossRef Full Text | Google Scholar

65. Bauman SK, Huffnagle GB, Murphy JW. Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response. Infect Immun. (2003) 71:68–74. doi: 10.1128/IAI.71.1.68-74.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

66. Osterholzer JJ, Milam JE, Chen GH, Toews GB, Huffnagle GB, Olszewski MA. Role of dendritic cells and alveolar macrophages in regulating early host defense against pulmonary infection with Cryptococcus neoformans. Infect Immun. (2009) 77:3749–58. doi: 10.1128/IAI.00454-09

PubMed Abstract | CrossRef Full Text | Google Scholar

67. Osterholzer JJ, Curtis JL, Polak T, Ames T, Chen GH, McDonald R, et al. CCR2 mediates conventional dendritic cell recruitment and the formation of bronchovascular mononuclear cell infiltrates in the lungs of mice infected with Cryptococcus neoformans. J Immunol. (2008) 181:610–20. doi: 10.4049/jimmunol.181.1.610

PubMed Abstract | CrossRef Full Text | Google Scholar

68. Hill JO, Harmsen AG. Intrapulmonary growth and dissemination of an avirulent strain of Cryptococcus neoformans in mice depleted of CD4+ or CD8+ T cells. J Exp Med. (1991) 173:755–8. doi: 10.1084/jem.173.3.755

CrossRef Full Text | Google Scholar

69. Huffnagle GB, Yates JL, Lipscomb MF. T cell-mediated immunity in the lung: a Cryptococcus neoformans pulmonary infection model using SCID and athymic nude mice. Infect Immun. (1991) 59:1423–33.

PubMed Abstract | Google Scholar

70. Huffnagle GB, Lipscomb MF, Lovchik JA, Hoag KA, Street NE. The role of CD4+ and CD8+ T cells in the protective inflammatory response to a pulmonary cryptococcal infection. J Leukoc Biol. (1994) 55:35–42. doi: 10.1002/jlb.55.1.35

PubMed Abstract | CrossRef Full Text | Google Scholar

71. Retini C, Kozel TR, Pietrella D, Monari C, Bistoni F, Vecchiarelli A. Interdependency of interleukin-10 and interleukin-12 in regulation of T-cell differentiation and effector function of monocytes in response to stimulation with Cryptococcus neoformans. Infect Immun. (2001) 69:6064–73. doi: 10.1128/IAI.69.10.6064-6073.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

72. Mukaremera L, Nielsen K. Adaptive Immunity to Cryptococcus neoformans infections. J Fungi. (2017). 3:64. doi: 10.3390/jof3040064

PubMed Abstract | CrossRef Full Text | Google Scholar

73. Goldman DL, Casadevall A, Cho Y, Lee SC. Cryptococcus neoformans meningitis in the rat. Lab Invest. (1996) 75:759–70.

PubMed Abstract | Google Scholar

74. Decken K, Kohler G, Palmer-Lehmann K, Wunderlin A, Mattner F, Magram J, et al. Interleukin-12 is essential for a protective Th1 response in mice infected with Cryptococcus neoformans. Infect Immun. (1998) 66:4994–5000.

PubMed Abstract | Google Scholar

75. Hidore MR, Nabavi N, Reynolds CW, Henkart PA, Murphy JW. Cytoplasmic components of natural killer cells limit the growth of Cryptococcus neoformans. J Leukoc Biol. (1990) 48:15–26. doi: 10.1002/jlb.48.1.15

PubMed Abstract | CrossRef Full Text | Google Scholar

76. Ma LL, Spurrell JC, Wang JF, Neely GG, Epelman S, Krensky AM, et al. CD8 T cell-mediated killing of Cryptococcus neoformans requires granulysin and is dependent on CD4 T cells and IL-15. J Immunol. (2002) 169:5787–95. doi: 10.4049/jimmunol.169.10.5787

PubMed Abstract | CrossRef Full Text | Google Scholar

77. Kleinschek MA, Muller U, Brodie SJ, Stenzel W, Kohler G, Blumenschein WM, et al. IL-23 enhances the inflammatory cell response in Cryptococcus neoformans infection and induces a cytokine pattern distinct from IL-12. J Immunol. (2006) 176:1098–106. doi: 10.4049/jimmunol.176.2.1098

PubMed Abstract | CrossRef Full Text | Google Scholar

78. Muller U, Stenzel W, Kohler G, Werner C, Polte T, Hansen G, et al. IL-13 induces disease-promoting type 2 cytokines, alternatively activated macrophages and allergic inflammation during pulmonary infection of mice with Cryptococcus neoformans. J Immunol. (2007) 179:5367–77. doi: 10.4049/jimmunol.179.8.5367

PubMed Abstract | CrossRef Full Text

79. Voelz K, Lammas DA, May RC. Cytokine signaling regulates the outcome of intracellular macrophage parasitism by Cryptococcus neoformans. Infect Immun. (2009) 77:3450–7. doi: 10.1128/IAI.00297-09

PubMed Abstract | CrossRef Full Text | Google Scholar

80. Vecchiarelli A, Pericolini E, Gabrielli E, Chow SK, Bistoni F, Cenci E, et al. Cryptococcus neoformans galactoxylomannan is a potent negative immunomodulator, inspiring new approaches in anti-inflammatory immunotherapy. Immunotherapy. (2011) 3:997–1005. doi: 10.2217/imt.11.86

PubMed Abstract | CrossRef Full Text | Google Scholar

81. Lu YK, Sun KH, Shen WC. Blue light negatively regulates the sexual filamentation via the Cwc1 and Cwc2 proteins in Cryptococcus neoformans. Mol Microbiol. (2005) 56:480–91. doi: 10.1111/j.1365-2958.2005.04549.x

PubMed Abstract | CrossRef Full Text | Google Scholar

82. Diamond RD, Bennett JE. Disseminated cryptococcosis in man: decreased lymphocyte transformation in response to Cryptococcus neoformans. J Infect Dis. (1973) 127:694–7. doi: 10.1093/infdis/127.6.694

PubMed Abstract | CrossRef Full Text | Google Scholar

83. Feldmesser M, Kress Y, Novikoff P, Casadevall A. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect Immun. (2000) 68:4225–37. doi: 10.1128/IAI.68.7.4225-4237.2000

PubMed Abstract | CrossRef Full Text | Google Scholar

84. Nosanchuk JD, Casadevall A. Cellular charge of Cryptococcus neoformans: contributions from the capsular polysaccharide, melanin, and monoclonal antibody binding. Infect Immun. (1997) 65:1836–41.

PubMed Abstract | Google Scholar

85. Collins HL, Bancroft GJ. Encapsulation of Cryptococcus neoformans impairs antigen-specific T-cell responses. Infect Immun. (1991) 59:3883–8.

PubMed Abstract | Google Scholar

86. Park YD, Shin S, Panepinto J, Ramos J, Qiu J, Frases S, et al. A role for LHC1 in higher order structure and complement binding of the Cryptococcus neoformans capsule. PLoS Pathog. (2014) 10:e1004037. doi: 10.1371/journal.ppat.1004037

PubMed Abstract | CrossRef Full Text | Google Scholar

87. Retini C, Vecchiarelli A, Monari C, Tascini C, Bistoni F, Kozel TR. Capsular polysaccharide of Cryptococcus neoformans induces proinflammatory cytokine release by human neutrophils. Infect Immun. (1996) 64:2897–903.

PubMed Abstract | Google Scholar

88. Walenkamp AM, Chaka WS, Verheul AF, Vaishnav VV, Cherniak R, Coenjaerts FE, et al. Cryptococcus neoformans and its cell wall components induce similar cytokine profiles in human peripheral blood mononuclear cells despite differences in structure. FEMS Immunol Med Microbiol. (1999) 26:309–18. doi: 10.1111/j.1574-695X.1999.tb01403.x

PubMed Abstract | CrossRef Full Text | Google Scholar

89. Vecchiarelli A, Retini C, Monari C, Tascini C, Bistoni F, Kozel TR. Purified capsular polysaccharide of Cryptococcus neoformans induces interleukin-10 secretion by human monocytes. Infect Immun. (1996) 64:2846–9.

PubMed Abstract | Google Scholar

90. Villena SN, Pinheiro RO, Pinheiro CS, Nunes MP, Takiya CM, DosReis GA, et al. Capsular polysaccharides galactoxylomannan and glucuronoxylomannan from Cryptococcus neoformans induce macrophage apoptosis mediated by Fas ligand. Cell Microbiol. (2008) 10:1274–85. doi: 10.1111/j.1462-5822.2008.01125.x

PubMed Abstract | CrossRef Full Text | Google Scholar

91. Blackstock R, Buchanan KL, Adesina AM, Murphy JW. Differential regulation of immune responses by highly and weakly virulent Cryptococcus neoformans isolates. Infect Immun. (1999) 67:3601–9.

PubMed Abstract | Google Scholar

92. Mirshafiey A, Ngoc Dzung DT, Murphy JW, Khorramizadeh MR, Saadat F, Mehrabian F, et al. Tolerability and anti-inflammatory effects of glucuronoxylomannan in collagen-induced arthritis. Scand J Immunol. (2004) 60:226–32. doi: 10.1111/j.0300-9475.2004.01458.x

PubMed Abstract | CrossRef Full Text | Google Scholar

93. Rocha JD, Nascimento MT, Decote-Ricardo D, Corte-Real S, Morrot A, Heise N, et al. Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils. Sci Rep. (2015) 5:8008. doi: 10.1038/srep08008

PubMed Abstract | CrossRef Full Text | Google Scholar

94. Monari C, Pericolini E, Bistoni G, Casadevall A, Kozel TR, Vecchiarelli A. Cryptococcus neoformans capsular glucuronoxylomannan induces expression of fas ligand in macrophages. J Immunol. (2005) 174:3461–8. doi: 10.4049/jimmunol.174.6.3461

PubMed Abstract | CrossRef Full Text | Google Scholar

95. Monari C, Paganelli F, Bistoni F, Kozel TR, Vecchiarelli A. Capsular polysaccharide induction of apoptosis by intrinsic and extrinsic mechanisms. Cell Microbiol. (2008) 10:2129–37. doi: 10.1111/j.1462-5822.2008.01196.x

PubMed Abstract | CrossRef Full Text | Google Scholar

96. Gougeon ML, Montagnier L. Programmed cell death as a mechanism of CD4 and CD8 T cell deletion in AIDS. Molecular control and effect of highly active anti-retroviral therapy. Ann N Y Acad Sci. (1999) 887:199–212. doi: 10.1111/j.1749-6632.1999.tb07934.x

PubMed Abstract | CrossRef Full Text | Google Scholar

97. Grechi J, Marinho-Carvalho M, Zancan P, Cinelli LP, Gomes AM, Rodrigues ML, et al. Glucuronoxylomannan from Cryptococcus neoformans down-regulates the enzyme 6-phosphofructo-1-kinase of macrophages. J Biol Chem. (2011) 286:14820–9. doi: 10.1074/jbc.M110.177030

PubMed Abstract | CrossRef Full Text | Google Scholar

98. Chiapello LS, Baronetti JL, Aoki MP, Gea S, Rubinstein H, Masih DT. Immunosuppression, interleukin-10 synthesis and apoptosis are induced in rats inoculated with Cryptococcus neoformans glucuronoxylomannan. Immunology. (2004) 113:392–400. doi: 10.1111/j.1365-2567.2004.01970.x

PubMed Abstract | CrossRef Full Text | Google Scholar

99. Chiapello LS, Aoki MP, Rubinstein HR, Masih DT. Apoptosis induction by glucuronoxylomannan of Cryptococcus neoformans. Med Mycol. (2003) 41:347–53. doi: 10.1080/1369378031000137260

PubMed Abstract | CrossRef Full Text | Google Scholar

100. Chiapello L, Iribarren P, Cervi L, Rubinstein H, Masih DT. Mechanisms for induction of immunosuppression during experimental cryptococcosis: role of glucuronoxylomannan. Clin Immunol. (2001) 100:96–106. doi: 10.1006/clim.2001.5046

PubMed Abstract | CrossRef Full Text | Google Scholar

101. Kuck D, Kolmerer B, Iking-Konert C, Krammer PH, Stremmel W, Rudi J. Vacuolating cytotoxin of Helicobacter pylori induces apoptosis in the human gastric epithelial cell line AGS. Infect Immun. (2001) 69:5080–7. doi: 10.1128/IAI.69.8.5080-5087.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

102. Luder CG, Gross U, Lopes MF. Intracellular protozoan parasites and apoptosis: diverse strategies to modulate parasite-host interactions. Trends Parasitol. (2001) 17:480–6. doi: 10.1016/S1471-4922(01)02016-5

PubMed Abstract | CrossRef Full Text | Google Scholar

103. Chaka W, Verheul AF, Vaishnav VV, Cherniak R, Scharringa J, Verhoef J, et al. Cryptococcus neoformans and cryptococcal glucuronoxylomannan, galactoxylomannan, and mannoprotein induce different levels of tumor necrosis factor alpha in human peripheral blood mononuclear cells. Infect Immun. (1997) 65:272–8.

PubMed Abstract | Google Scholar

104. Delfino D, Cianci L, Lupis E, Celeste A, Petrelli ML, Curro F, et al. Interleukin-6 production by human monocytes stimulated with Cryptococcus neoformans components. Infect Immun. (1997) 65:2454–6.

PubMed Abstract | Google Scholar

105. Siddiqui AA, Shattock RJ, Harrison TS. Role of capsule and interleukin-6 in long-term immune control of Cryptococcus neoformans infection by specifically activated human peripheral blood mononuclear cells. Infect Immun. (2006) 74:5302–10. doi: 10.1128/IAI.00661-06

PubMed Abstract | CrossRef Full Text | Google Scholar

106. Pericolini E, Cenci E, Monari C, De Jesus M, Bistoni F, Casadevall A, et al. Cryptococcus neoformans capsular polysaccharide component galactoxylomannan induces apoptosis of human T-cells through activation of caspase-8. Cell Microbiol. (2006) 8:267–75. doi: 10.1111/j.1462-5822.2005.00619.x

PubMed Abstract | CrossRef Full Text | Google Scholar

107. Moyrand F, Fontaine T, Janbon G. Systematic capsule gene disruption reveals the central role of galactose metabolism on Cryptococcus neoformans virulence. Mol Microbiol. (2007) 64:771–81. doi: 10.1111/j.1365-2958.2007.05695.x

PubMed Abstract | CrossRef Full Text | Google Scholar

108. LaRocque-de-Freitas IF, Rocha JDB, Nunes MP, Oliveira PAV, Nascimento DO, Freire-de-Lima L, et al. Involvement of the capsular GalXM-induced IL-17 cytokine in the control of Cryptococcus neoformans infection. Sci Rep. (2018) 8:16378. doi: 10.1038/s41598-018-34649-4

PubMed Abstract | CrossRef Full Text | Google Scholar

109. Li LX, Rautengarten C, Heazlewood JL, Doering TL. Xylose donor transport is critical for fungal virulence. PLoS Pathog. (2018) 14:e1006765. doi: 10.1371/journal.ppat.1006765

PubMed Abstract | CrossRef Full Text | Google Scholar

110. Heise N, Gutierrez AL, Mattos KA, Jones C, Wait R, Previato JO, et al. Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms. Glycobiology. (2002) 12:409–20. doi: 10.1093/glycob/cwf053

PubMed Abstract | CrossRef Full Text | Google Scholar

111. Gutierrez AL, Farage L, Melo MN, Mohana-Borges RS, Guerardel Y, Coddeville B, et al. Characterization of glycoinositolphosphoryl ceramide structure mutant strains of Cryptococcus neoformans. Glycobiology. (2007) 17:1–11C. doi: 10.1093/glycob/cwm030

PubMed Abstract | CrossRef Full Text | Google Scholar

112. Moyrand F, Klaproth B, Himmelreich U, Dromer F, Janbon G. Isolation and characterization of capsule structure mutant strains of Cryptococcus neoformans. Mol Microbiol. (2002) 45:837–49. doi: 10.1046/j.1365-2958.2002.03059.x

PubMed Abstract | CrossRef Full Text | Google Scholar

113. Moyrand F, Chang YC, Himmelreich U, Kwon-Chung KJ, Janbon G. Cas3p belongs to a seven-member family of capsule structure designer proteins. Eukaryot Cell. (2004) 3:1513–24. doi: 10.1128/EC.3.6.1513-1524.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

114. Kozel TR, Levitz SM, Dromer F, Gates MA, Thorkildson P, Janbon G. Antigenic and biological characteristics of mutant strains of Cryptococcus neoformans lacking capsular O acetylation or xylosyl side chains. Infect Immun. (2003) 71:2868–75. doi: 10.1128/IAI.71.5.2868-2875.2003

CrossRef Full Text | Google Scholar

115. Klutts JS, Levery SB, Doering TL. A beta-1,2-xylosyltransferase from Cryptococcus neoformans defines a new family of glycosyltransferases. J Biol Chem. (2007) 282:17890–9. doi: 10.1074/jbc.M701941200

PubMed Abstract | CrossRef Full Text | Google Scholar

116. Klutts JS, Doering TL. Cryptococcal xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans plays a direct role in the synthesis of capsule polysaccharides. J Biol Chem. (2008) 283:14327–34. doi: 10.1074/jbc.M708927200

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: Cryptococcus neoformans, glucuronoxylomannan (GXM), galactoxylomannan (GalXM), immunomodulation, immuno evasion

Citation: Decote-Ricardo D, LaRocque-de-Freitas IF, Rocha JDB, Nascimento DO, Nunes MP, Morrot A, Freire-de-Lima L, Previato JO, Mendonça-Previato L and Freire-de-Lima CG (2019) Immunomodulatory Role of Capsular Polysaccharides Constituents of Cryptococcus neoformans. Front. Med. 6:129. doi: 10.3389/fmed.2019.00129

Received: 13 February 2019; Accepted: 24 May 2019;
Published: 19 June 2019.

Edited by:

Miguel Cacho Teixeira, University of Lisbon, Portugal

Reviewed by:

John Bennett, National Institute of Allergy and Infectious Diseases (NIAID), United States
Bettina Fries, Stony Brook Medicine, United States
Carolina Coelho, University of Exeter, United Kingdom

Copyright © 2019 Decote-Ricardo, LaRocque-de-Freitas, Rocha, Nascimento, Nunes, Morrot, Freire-de-Lima, Previato, Mendonça-Previato and Freire-de-Lima. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Celio Geraldo Freire-de-Lima, celio@biof.ufrj.br