Original Research ARTICLE
Characterization of the adaptive amoxicillin resistance of Lactobacillus casei Zhang by proteomic analysis
- 1Inner Mongolia Agricultural University, Mongolia
Amoxicillin is one of the most commonly prescribed antibiotics for bacterial infections and gastrointestinal disorders. To investigate the adaptation of Lactobacillus (L.) casei Zhang to amoxicillin stress, an iTRAQ-based comparative proteomic analysis was performed to compare the protein profiles between the parental L. casei Zhang and its amoxicillin-resistant descendent strains. Our results revealed a significant increase in the relative expression of 38 proteins (>2.0 folds, P<0.05), while the relative expression of 34 proteins significantly decreased (<-2.0 folds, P<0.05). The amoxicillin-resistant descendent strain exhibited marked alterations in carbohydrate and amino acid metabolism. Moreover, certain components involving in membrane metabolism were activated. The differences in the proteomic profiles between the two strains might explain the enhanced stress resistance of the adapted bacteria.
Keywords: Lactobacillus casei Zhang, Proteomic analysis, Amoxicillin, adaptation, Survival
Received: 24 Jun 2017;
Accepted: 07 Feb 2018.
Edited by:Abd El-Latif Hesham, Assiut University, Egypt
Reviewed by:Weili Liang, National Institute for Communicable Disease Control and Prevention (China CDC), China
Zhao Chen, University of California, Davis, United States
Copyright: © 2018 Wang, Guo, Cao, Kwok, Zhang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Wenyi Zhang, Inner Mongolia Agricultural University, Hohhot, Mongolia, firstname.lastname@example.org