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Front. Microbiol. | doi: 10.3389/fmicb.2018.00303

Integrated lung and tracheal mRNA-seq and miRNA-seq analysis of dogs with an avian-like H5N1 canine influenza virus infection

 Cheng Fu1, jie luo1,  shaotang ye1, ziguo yuan1 and  Shoujun Li1*
  • 1College of Veterinary Medicine, South China Agricultural University, China

Avian-like H5N1 canine influenza virus (CIV) causes severe respiratory infections in dogs. However, the mechanism underlying H5N1 CIV infection in dogs is unknown. The present study aimed to identify differentially expressed miRNAs and mRNAs in the lungs and trachea in H5N1 CIV-infected dogs through a next-generation sequencing-based method. Eighteen 40-day-old beagles were inoculated intranasally with CIV, A/canine/01/Guangdong/2013 (H5N1) at a tissue culture infectious dose 50 (TCID50) of 106, and lung and tracheal tissues were harvested at 3 and 7 d post-inoculation (dpi). The tissues were processed for miRNA and mRNA analysis. By means of miRNA-gene expression integrative negative analysis, we found miRNA-mRNA pairs. Lung and trachea tissues showed 138 and 135 negative miRNA-mRNA pairs, respectively. One hundred and twenty negative miRNA-mRNA pairs were found between the different tissues. In particular, pathways including the influenza A pathway, chemokine signaling pathways, and the PI3K-Akt signaling pathway were significantly enriched in all groups in responses to virus infection. Furthermore, dysregulation of miRNA and mRNA expression was observed in the respiratory tract of H5N1 CIV-infected dogs and notably, TLR4 (miR-146), NF-KB (miR-34c) and CCL5 (miR-335), CCL10 (miR-8908-5p), and GNGT2 (miR-122) were found to play important roles in regulating pathways that resist virus infection. To our knowledge, the present study is the first to analyze miRNA and mRNA expression in H5N1 CIV-infected dogs; furthermore, the present findings provide insights into the molecular mechanisms underlying influenza virus infection.

Keywords: anine influenza virus, H5N1, mRNA-miRNA integrate analysis, negative, KEGG

Received: 13 Oct 2017; Accepted: 09 Feb 2018.

Edited by:

Akio Adachi, Tokushima University, Japan

Reviewed by:

Meilin Jin, Huazhong Agricultural University, China
Luis Villarreal, Center for Emeriti & Retirees, University of California, United States
Weifeng Shi, Taishan Medical University, China
John Pasick, Canadian Food Inspection Agency, Canada  

Copyright: © 2018 Fu, luo, ye, yuan and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Shoujun Li, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China, shoujunli@scau.edu.cn