Original Research ARTICLE
The Porphyromonas gingivalis/Host Interactome Shows Enrichment in GWASdb Genes Related to Alzheimer's Disease, Diabetes and Cardiovascular Diseases
- 1Polygenic Pathways, Hastings, United Kingdom
- 2Dementia and Neurodegenerative Diseases Research Group, Faculty of Clinical and Biomedical Sciences, School of Dentistry, University of Central Lancashire, Preston, United Kingdom
Periodontal disease is of established etiology in which polymicrobial synergistic ecology has become dysbiotic under the influence of Porphyromonas gingivalis. Following breakdown of the host's protective oral tissue barriers, P. gingivalis migrates to developing inflammatory pathologies that associate with Alzheimer's disease (AD). Periodontal disease is a risk factor for cardiovascular disorders (CVD), type II diabetes mellitus (T2DM), AD and other chronic diseases, whilst T2DM exacerbates periodontitis. This study analyzed the relationship between the P. gingivalis/host interactome and the genes identified in genome-wide association studies (GWAS) for the aforementioned conditions using data from GWASdb (P < 1E-03) and, in some cases, from the NCBI/EBI GWAS database (P < 1E-05). Gene expression data from periodontitis or P. gingivalis microarray was compared to microarray datasets from the AD hippocampus and/or from carotid artery plaques. The results demonstrated that the host genes of the P. gingivalis interactome were significantly enriched in genes deposited in GWASdb genes related to cognitive disorders, AD and dementia, and its co-morbid conditions T2DM, obesity, and CVD. The P. gingivalis/host interactome was also enriched in GWAS genes from the more stringent NCBI-EBI database for AD, atherosclerosis and T2DM. The misregulated genes in periodontitis tissue or P. gingivalis infected macrophages also matched those in the AD hippocampus or atherosclerotic plaques. Together, these data suggest important gene/environment interactions between P. gingivalis and susceptibility genes or gene expression changes in conditions where periodontal disease is a contributory factor.
Complex chronic diseases such as periodontitis, cardiovascular disease (CVD; including atherosclerosis, strokes, hypertension, myocardial infarction, congestive heart failure), type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD), are increasingly common during advanced aging and hence place a considerable social and economic burden globally. Porphyromonas gingivalis is a risk factor for periodontitis and is associated with distal inflammatory pathologies including CVD, T2DM, and AD via immune modification mechanisms. The prevalence of both periodontitis and AD increases with aging (Silvestre et al., 2017) as do CVD (Qiu and Fratiglioni, 2015) and T2DM (Yakaryilmaz and Ozturk, 2017). The aging process itself may contribute to these conditions, particularly in relation to pathogens, via immunosenescence. This can decrease resistance to pathogens due to immunodeficiency but is also accompanied by an increase in the pro-inflammatory activity of monocytes and macrophages which can lead to chronic low grade, inflammation, termed “inflammageing,” which is also associated with AD, CVD, and T2DM (Fülöp et al., 2016).
Periodontitis is of particular interest because of its known polymicrobial etiology and a recognized oral microbiome (Socransky et al., 1998; Aas et al., 2005). The Human Oral Microbiome Database www.homd.org (Chen et al., 2010). An uncontrolled oral microbiome may act as a reservoir, from which opportunistic pathogens can migrate to remote body organs. P. gingivalis and oral spirochetes for example, associate with extra-oral niches (Riviere et al., 2002; Poole et al., 2013; Olsen and Progulske-Fox, 2015). Periodontal pathogens enter the systemic system through daily bacteraemia following breakdown of epithelial: endothelial barriers due to the host's inflammatory responses and the ability of some pathogens, including P. gingivalis to attack these barriers (Katz et al., 2000).
Chronic periodontitis is associated with the sporadic form of AD and other related comorbidities, T2DM, and atherosclerosis inter alia (Löe, 1981, 1993; Olsen and Singhrao, 2015; Singhrao et al., 2015; Olsen et al., 2016; Bale et al., 2017; Harding et al., 2017). Here our focus is on P. gingivalis as the keystone pathogen, because it is by far the best-researched bacterium for its contribution to periodontitis (Hajishengallis et al., 2012; Hajishengallis and Lamont, 2014; Olsen et al., 2016).
Periodontal disease is an inflammatory condition affecting the tissues supporting teeth in their bony socket and occurs in aggressive and chronic subtypes. The disease is caused by polymicrobial dysbiosis with several bacterial species playing a significant role in tooth loss (Hajishengallis and Lamont, 2014; Olsen et al., 2016). Some of these include Actinomycetem actinomycetemcomitans, and those belonging to the red complex (P. gingivalis, Tannerella Forsythia, Treponema denticola). Others are intermediate colonizers of the sub-gingival biofilm ecology of the orange complex, (Fusobacterium nucleatum, Peptostreptococcus micros, Prevotella intermedia, Prevotella nigrecens, Eubacterium nodatum, and Streptococcus constellates) (Haffajee et al., 2008) and other species. Together they contribute to pathological periodontal pocket formation around the tooth.
The importance of P. gingivalis is the bacterium's ability to subvert the roles of organ specific inflammatory cells via a number of virulence factors, the most important being its lipopolysaccharide (LPS) and gingipains (Singhrao et al., 2015; How et al., 2016). Once in the new niche, P. gingivalis induces dysbiosis of local commensals (Harding et al., 2017) and drives immune reactions in favor of inflammatory amplification whilst maintaining chronic disease (Olsen et al., 2017). The inflammatory contribution from periodontitis links to CVD (atherosclerosis), T2DM and AD and other pathologies. The American Heart Association (AHA) declared, after an extensive review of the literature, that periodontal disease was independently associated with arteriosclerotic vascular disease (ASVD) (Bale et al., 2017). Similarly, T2DM has been recognized as a complication of periodontal disease (Löe, 1993) and periodontitis has been proposed as a risk factor for AD (Kamer et al., 2015; Harding et al., 2017; Leira et al., 2017) with support from longitudinal monitoring documented elsewhere (Chen et al., 2017).
AD is associated with impaired cognition and a number of pathological lesions, classically amyloid-beta (Aβ) deposits and hyperphosphorylated neurofibrillary tangles (Goedert et al., 1991). Many pathogens, (e.g., herpes simplex type 1 (HSV-1), Chlamydia pneumoniae, Borrelia burgdorferi) (Itzhaki et al., 2016) as well as P. gingivalis lipopolysaccharide (Wu et al., 2017) are able to promote Aβ deposition. Aβ has broad-spectrum antimicrobial effects against bacteria, fungi and viruses (Soscia et al., 2010; White et al., 2014; Bourgade et al., 2015; Kumar et al., 2016) and its deposition may result from an innate immune response to the cerebral invasion of pathogens that have been associated with AD.
T2DM is a metabolic disorder characterized by hyperglycemia and insulin resistance. The complications of diabetes result from long-term elevation of blood glucose levels. Diabetes has significant impact on gingival and periodontal tissues due to poor glycaemic control (Löe, 1981). A consequence of hyperglycaemia is that free sugars circulating in the blood give rise to advanced glycation end-products (AGEs), which are a unique inflammatory product. Endothelial cells and monocytes have receptors that bind AGE products. This in turn has an impact on gingival tissue vascular permeability through enhanced breakdown of the periodontium by protease activity of polymicrobial infections (Embery et al., 2000; Sugiyama et al., 2012). T2DM is a well-established risk factor for stroke (Chen et al., 2016) and as a complication of periodontal disease (Löe, 1981). As with AD, numerous (and similar) oral pathogens have been associated in patients with diabetes (Castrillon et al., 2015). The pancreatic amyloid (amylin) accumulates in pancreatic islets in T2DM. Amylin also has antimicrobial effects and its accumulation may result from an innate immune response to chronic bacterial infections, ultimately triggering the inflammatory pathology related specifically to T2DM (Miklossy and McGeer, 2016).
Periodontitis is associated with age-related diseases such as atherosclerosis via immune processes leading to dyslipidaemia in the vessel walls (Libby et al., 2002; Velsko et al., 2014). These events follow from established periodontitis (Socransky et al., 1998). One feature of the polymicrobial infection is that P. gingivalis secretes a peptidyl arginine deiminase enzyme that can modify proteins prevalent in atherosclerotic lesions by citrullination (Janssen et al., 2013; Sokolove et al., 2013; Geraldino-Pardilla et al., 2017). Gearldino-Pardilla et al. reported that higher levels of autoantibodies against citrullinated proteins can target citrullinated histone 2B associated with higher coronary artery calcium scores (amount of calcium in walls of arteries supplying heart muscle) when compared with lower antibody levels, suggesting a potential role of seroreactivity to citrullinated histone in atherosclerosis (Geraldino-Pardilla et al., 2017).
All of the aforementioned diseases have both genetic and environmental components, the latter often related to pathogens, as is the case for AD (see above), atherosclerosis (Sessa et al., 2014), and T2DM (Chakraborty et al., 2017). Previous studies have shown that host genes utilized by oncogenic viruses relate to cancer susceptibility genes (Rozenblatt-Rosen et al., 2012) and several genes related to AD are involved in the life cycles of the many pathogens implicated in this disorder (HSV-1, C. pneumoniae, Cryptococcus neoformans, B. burgdorferi, Helicobacter pylori, and P. gingivalis) (Carter, 2011). The HSV-1 and the parasite Toxoplasma Gondii—host interactomes also overlap with the susceptibility genes of a variety of neurological and psychiatric diseases (Carter, 2013a,b). A further study of 110 viruses has shown host/pathogen and host/host interactome overlaps that are relevant to the genetics of multiple human diseases (Navratil et al., 2011). These results support the notion of important relationships between the genes and proteins used by pathogens and disease susceptibility genes. These gene/environment interactions are likely to affect disease manifestation.
In this study, we have compared the P. gingivalis/host interactome with GWAS susceptibility genes involved in AD, T2DM and related metabolic (obesity) syndromes, and atherosclerosis (CVD, strokes). We have also compared the gene expression profiles derived from periodontitis gingival tissue or P. gingivalis-treated macrophages with those derived from AD hippocampal tissue or atherosclerotic plaques from clinical samples.
The P. gingivalis/host interactome (currently comprised of 3,993 host genes) curated manually, from Pubmed references is available at http://www.polygenicpathways.co.uk/pgingivalis.htm, together with a KEGG pathway analysis of the host arm of this interactome.
Susceptibility genes for a variety of diseases were from two genome-wide association study (GWAS) databases. The first of these (GWASdb, http://jjwanglab.org/gwasdb); contains genes with a P-value cut-off of P < 1E-10−3 (Li et al., 2012). (The file downloaded was from the August 2015 release (download GWASdb SNP-Trait file). The second is the more conservative NHGRI-EBI Catalog (P < 1E × 10−5) posted at (NHGRI-EBI Catalog, https://www.ebi.ac.uk/gwas/docs) accessed in May 2017 (Macarthur et al., 2017) and this was used for selected diseases derived from the initial GWASdb analysis. The reported genes column represents data from the NHGRI-EBI catalog. For AD only, genes labeled as “Alzheimer's disease,” or “Alzheimer's disease (late onset),” were used. GWASdb contains data from 591 disease entities classified as disease ontology identifier (DOIDs) according to Disease-Ontology Lite (http://disease-ontology.org/) (Kibbe et al., 2015). However, many of the keywords used for the searches were general (for example, brain disease, cardiovascular system disease) and these were ignored in favor of more specific identities of interest (e.g., Alzheimer's disease, atherosclerosis, myocardial infarction), (N = 285). In addition, we compared the P. gingivalis interactome with the proteome of mouse cerebral arteries from the circle of Willis (6,630 proteins) (Badhwar et al., 2014). The proteins from this area of the anatomy is relevant because arteries supplying blood to the brain branch out at the circle of Willis and are subject to atherosclerosis in AD (Roher et al., 2003).
Next, we compared the microarray gene expression studies for periodontitis or P. gingivalis with the microarray datasets from AD and/or with atherosclerosis. The periodontitis microarray data are from an integrated analysis of three microarray datasets from human gingival tissue obtained from periodontitis patients (Guo et al., 2015). The effects of P. gingivalis relate to a microarray study of human macrophages exposed to live P. gingivalis, its LPS, or its fimbrial component (fimbrillin = FimA), 2h post infection or treatment; (Affymetrix Human Genome U133 Plus 2.0 array) (Zhou and Amar, 2007). These effects (periodontitis or P.gingivalis microarrays), were compared with microarray datasets obtained from post-mortem studies of the AD hippocampus for incipient and/or established AD (nine control and 22 AD subjects of varying severity on 31 separate microarrays, Affymetrix Human Genome U133A) (Blalock et al., 2004); and from a study comparing stable vs. unstable atherosclerotic plaques derived from human carotid endarterectomy specimens (Total RNA from 11 segments from 3 atherosclerotic plaques classified as stable and unstable: Affymetrix Human Genome U133). The expression data refer to stable/unstable atherosclerotic plaques comparisons (Papaspyridonos et al., 2006). Gene symbols for all data conform to the Human gene Nomenclature (HUGO) system (Povey et al., 2001). Overlapping gene symbols from the comparisons were with the Venny tool, online at the following URL (http://bioinfogp.cnb.csic.es/tools/venny/) (Oliveros, 2007).
Assuming a human genome of 26,846 coding genes and a P. gingivalis interactome of 3,993 host genes, 3,993/26,846 gene sets would be expected in any comparator GWAS or other dataset (14.87%). This calculation allowed the determination of the expected values and the enrichment values (observed/expected) in relation to the GWASdb or NHGRI-EBI datasets. The same approach was used to compare microarray data, using the number of misregulated disease genes (= N) to define expected percentage overlap values (= N/26846%). Statistical significance of the enrichment was calculated using the hypergeometric probability test where a Bonferroni cut-off (0.05 × 591 = < 0.0000846) was applied. The resultant p-values from each of the analyzed series, was corrected for, by accounting for the false discovery rate (FDR p = q) (Benjamini and Hochberg, 1995). Significant FDR corrected values are considered at q < 0.05. The KEGG Pathway enrichment analysis was determined using the Consensus Path database (CPDB) at http://cpdb.molgen.mpg.de/ (Herwig et al., 2016).
The P. gingivalis interactome was highly enriched in GWASdb genes related to neurological disorders (cognitive disorder, dementia and AD), and with metabolic disorders (T2DM and obesity). Each of these conditions has been associated with periodontal disease, as referenced in Table 1. The analysis showed P. gingivalis bacterial interactome enrichment in GWASdb genes for CVD including those for hypertension, diverse atherosclerotic conditions, myocardial infarction and congestive heart failure. Similarly, the interactome also related to GWASdb genes from several psychiatric conditions or substance abuse-related disorders that link to periodontal disease (as referenced in Table 1); and are related to mood, depression, anxiety and sleep disorders as well as to substance dependence, including nicotine and alcohol dependency (Table 1). Such a finding was further strengthened by interactome enrichment confirmation in relation to the more stringent NHGRI-EBI GWAS database (P < 1E-05) for genes related to AD, T2DM, and atherosclerosis (= carotid plaque burden in the NHGRI-EBI GWAS database). Other diseases were not analyzed.
Table 1. The overlaps between the host genes of the P. gingivalis interactome with genes associated with diverse diseases from the GWASdb (top unshaded region) or the NCBI-EBI (bottom shaded region) databases.
The overlap between the P. gingivalis interactome and periodontitis/periodontal disease (GWASdb) was not significant for 51 GWASdb genes, but was nominally significant (p = 0.029) for 108 periodontitis genes from the NHGRI-EBI Catalog. This lack of, or low significance may relate to the fact that numerous other pathogens are involved in causing chronic periodontitis (Table 1). It could also be related to statistical power problems inherent to this type of analysis (a fixed number of the P. gingivalis interactome (N = 3993) and variable numbers of GWASdb susceptibility genes (from 51 in periodontitis to 4,763 in cognitive disorder).
A Focus on AD, T2DM, Atherosclerosis and Hypertension
The P. gingivalis interactome overlaps with GWASdb genes associated with each of these (AD, T2DM, atherosclerosis and hypertension) diseases or risk factors. Some of these interactome overlaps are with genes specific to either single diseases or risk factors (diabetes = N = 417; hypertension N = 193; AD = N = 131; atherosclerosis N = 39). In other cases, overlapping interactome genes are common to two or more diseases such as, AD/diabetes N = 105; AD/atherosclerosis N = 22; AD/atherosclerosis/diabetes N = 11; (AD/atherosclerosis/hypertension N = 5) (Figure 1). Twenty-three of seventy-eight AD genes from the more stringent NHGRI–EBI GWAS database (p < 1E-05) also appeared in these four datasets (Figure 1).
Figure 1. Venn diagram shows the number of susceptibility genes (GWASdb) and their percentage occurrence within the P. gingivalis/host interactome that are common or specific to various combinations of atherosclerosis (Athero), type 2 diabetes (Diab), hypertension (hyper) and Alzheimer's (Alz), The Venn diagram is for data from GWASdb. The genes in boxes refer to GWAS genes from the more stringent NCBI-EBI data that are also part of the P. gingivalis/host interactome. The percentages are those of the sum of the interactome/susceptibility gene overlaps for all diseases represented in the figure (atherosclerosis+ type 2 diabetes + hypertension+ Alzheimer's gene/interactome overlaps).
Over-Representation of Arterial Genes in the P. gingivalis Interactome
Of the full P. gingivalis interactome (3,993 genes), 1,423 belonged to the cerebral artery proteome dataset of 6,630 proteins (21.4%: Observed/expected = 1.44: enrichment: P = 7.2E-63). Of the 385 genes common to the P. gingivalis interactome and AD GWASdb genes, 175 belonged to the cerebral artery proteome: (45.3%; observed/expected = 1.85: enrichment P = 0).
KEGG Pathway Analysis of the AD Genes Common to the P. gingivalis Interactome
The analysis of pathways outlined by the GWASdb genes common to AD and the P. gingivalis interactome generated by the CPDB website, are in Table 2. (KEGG pathways for the entire host/pathogen interactome are posted at http://www.polygenicpathways.co.uk/pgingkegg.html. These genes include pathways related to cancers, AD, infection and immune responses, cytokine and chemokines and multiple metabolic signaling processes). KEGG pathway analysis of the common AD/interactome genes revealed pathways relevant to blood-brain barrier (BBB) function, including focal adhesion, junction and actin pathways, protein digestion and absorption (mainly represented by collagen genes that are related to vascular smooth muscle, and leukocyte trans endothelial migration).
Table 2. KEGG pathway enrichment analysis of the genes common to GWASdb AD genes and the P. gingivalis interactome.
Microarray Studies of Periodontitis or P. gingivalis: Comparison with Alzheimer's Disease and Atherosclerosis
The genes upregulated in periodontitis significantly overlapped with those upregulated in the AD hippocampus [mature AD (P = 3.9E-30), incipient AD (P = 4.6E-14)] and in atherosclerotic plaques (P = 1.7E-13) (Figure 2A). There was also significant match between the genes downregulated in periodontitis and those downregulated in mature AD (P = 5.6E-31), incipient AD (P = 3.5E-51) and atherosclerotic plaques (P = 9.8E-97) (Figure 2B).
Figure 2. (A,B) The number of overlapping genes and the significance of enrichment in comparisons of the effects of periodontitis in human oral tissue or P. gingivalis in human macrophages with microarrays from the AD hippocampus or from unstable atherosclerotic plaques (Athero). (A) Upregulated genes; (B) Down-regulated genes. Pdont, periodontitis; Pg live, live P. gingivalis; Pg LPS, P. gingivalis-lipopolysaccharide; Pg Fima, P. gingivalis fimbrial component. The labels on the X-axis (e.g., Pdont | 1.96 | 2371) correspond to these conditions, followed by observed/expected values, and the number of misregulated genes in the periodontitis or P. gingivalis microarrays. The maximum of the Y-axis (FDR q-value) is set at p = 0.05 and invisible points above this line are non-significant. No down-regulated data were available for down-regulated genes in the case of P. gingivalis LPS. The number of up or down-regulated genes in the Alzheimer's (Alz) or atherosclerosis datasets are also shown.
P. gingivalis Comparison
In general, the overlaps between the P. gingivalis microarrays (live, LPS, or FimA effects in macrophages) and the misregulated genes in AD or atherosclerosis were fewer and less significant, than when compared with periodontitis, where a more diverse microbial population is encountered (Figures 2A,B). The genes upregulated in AD significantly matched those upregulated by live P. gingivalis (mature AD: P = 1.1E-08: incipient AD: P = 0.0003), its LPS (mature AD: P = 0.0002: incipient AD: P = 0.005) or FimA (mature AD: P = 3.2E-07: incipient AD P = 0.0006) (Figure 2A). The genes downregulated in incipient AD matched those affected by live P. gingivalis infection (P = 1.1E-08) or its FimA (P = 3.4E-06) but the comparisons between mature AD and live P. gingivalis or its FimA component were not significant (Figure 2B).
The genes upregulated in atherosclerotic plaques also matched those affecting macrophages by live P. gingivalis (P = 0.0005) infection, or its FimA component (P = 0.013). The LPS comparison was not significant (P = 0.06). The effects of live P. gingivalis, were significant for the genes downregulated in the atherosclerosis comparison (P = 9.2E-10). The FimA comparison was not significant (P = 0.06) and no downregulated data were available for the bacterial LPS.
P. gingivalis is but one of several pathogens contributing to periodontal disease and this may contribute to the lack of (GWASdb) or low significance (NHGRI–EBI) of the overlap between the host/pathogen interactome and the genes associated with periodontitis itself. Many individuals do not develop periodontitis during their lifetime (Trombelli et al., 2004). Progression to the aggressive form of periodontitis is also rare and is associated with neutrophil defects with A. actinomycetemcomitans as the dominant etiological agent (Zambon, 1985), whereas chronic periodontitis is associated with complexes containing mixtures of species such as P. gingivalis, T. forsythia and a range of spirochetes (Socransky et al., 1998; Haffajee et al., 2008). Given the relationship between pathogens and host genes, as exemplified in this paper, such clinical variables may also depend upon the host's genetic profile. However, the enrichment of the interactome in genes associated with many other diseases linked to periodontitis (e.g., AD, CVD, and T2DM) was highly significant. This suggests that, within the sub-gingival microbiome, P gingivalis is a key pathogen contributing to the pathologies of these diseases. The focus of this paper relates principally to AD through these comorbid states.
The oral pathogen P. gingivalis interacts with or alters the expression of thousands of host genes forming an extensive host/pathogen interactome. The genes of the host arm of this interactome overlap with GWASdb genes associated with AD, atherosclerosis and T2DM and other CVD complications including hypertension and myocardial infarction. They also overlap with GWASdb genes associated with several psychiatric conditions and/or psychological factors determining life-styles including mood disorders, depression, anxiety and sleep disorders and substance abuse. Periodontitis can affect mood, depression, anxiety and sleep disorders as discussed previously (Harding et al., 2017) and the outcome of this interactome analysis supports this related conclusion. In addition, dependency on drugs including nicotine and excessive alcohol intake can negatively affect oral hygiene and periodontitis (Bonfim et al., 2013; Singh et al., 2013) and subsequently mental health as a bi-directional association (oral health < > mental health) (Kisely, 2016). The overlap between the genes associated with nicotine and alcohol abuse and the P. gingivalis interactome (Table 1) may relate to the effects of nicotine or alcohol on host genes affecting pathogen levels and virulence. For example, nicotine reduces the induction of the anti-microbial beta-defensin-2 induced by P. gingivalis LPS in human gingival epithelial cells (Mahanonda et al., 2009), and even low concentrations of cigarette smoke condensate increase invasion of human gingival epithelial cells by P. gingivalis (Imamura et al., 2015). In general, both alcoholism and smoking can modify cytokine responses to bacterial LPS or lipoteichoic acid in systemic immune cells (Gaydos et al., 2016).
Polymorphisms in human genes affect host physiology, but they are also likely to affect the interactions between pathogen and host. Pathogens also use these host genes/proteins during their life cycles, and the host employs many immune, inflammatory and defensive genes against the pathogens. Modifications in the host genes must also impinge on these effects. As previously discussed (Carter, 2013a,b), disease susceptibility genes relevant to pathogens may thus divert the effects of the pathogen toward or away from particular pathways, thus orientating its adverse effect to directions that enable it to influence diverse diseases in a manner that depends on the genetics of the host. The effects of P. gingivalis on colonization, biofilm formation, immune responses and endothelial cell activation are also strain-dependent (Walter et al., 2004; Belanger et al., 2008; Wilensky et al., 2009; Barbosa et al., 2015) and it is likely that distinct host gene relationships and disease relationships exist for strains with differing virulence.
In this study, the host genes of the bacterial interactome coincided with a high degree of significance with susceptibility genes related to cognitive disorders, AD and dementia, as well as with genes related to obesity and T2DM, and to those related to atherosclerosis and related disorders. This study adds significance to periodontal disease or P. gingivalis infection having associations with all of these conditions. The P. gingivalis interactome related to genes specifically or commonly associated with AD, atherosclerosis, diabetes and hypertension as depicted in the Venn diagram. These conditions are inter-related. For example diabetes (Shinohara and Sato, 2017), atherosclerosis (Hofman et al., 1997), and hypertension (de la Torre, 2006) are all associated with AD, as is obesity (Milionis et al., 2014). P. gingivalis may contribute to each condition in diverse ways via these host/pathogen interactions. The pathogen may thus influence AD risk both directly and via its influence on these other AD risk factors. There have been fewer studies relating periodontal disease to psychiatric disorders such as schizophrenia (McCreadie et al., 2004) but this study suggests that any such relationship may also have genetic components.
The relationship between periodontitis or P. gingivalis and AD or atherosclerosis is supported by the transcriptome analyses studies. The gene expression signatures in periodontal disease tissue or in macrophage responses to P. gingivalis components significantly matched those from the AD hippocampus or from atherosclerotic plaques in most cases, particularly in relation to the upregulated genes. In the case of the AD hippocampal transcriptome the upregulated genes contain the pathways relevant to pathogens and immune activation (inflammation, complement activation, and the defense response) (Blalock et al., 2004). The degree of significance was higher for the periodontal disease transcriptome and this may reflect the contribution of many other pathogens involved in periodontitis. AD (Itzhaki et al., 2016) and atherosclerosis (Sessa et al., 2014; Budzynski et al., 2016) have also been associated with multiple pathogens and recent microbiome studies have reported numerous bacterial and fungal species, some of oral origin, in the AD brain (Emery et al., 2017; Pisa et al., 2017). In relation to AD, the KEGG pathway analysis of the overlap between the interactome/AD genes provides some clues as to how P. gingivalis may contribute to AD. Many significantly affected pathways relate to breakdown of functional barriers and to the immune system and inflammatory pathways. The host genes of the bacterial interactome were also highly enriched in those found in the cerebral artery of the circle of Willis.
Periodontitis and atherosclerosis can be induced in apolipoprotein E knockout (ApoE−/−) mice (Velsko et al., 2014). We have reported such investigations (Poole et al., 2015; Singhrao et al., 2017). A recent study using the same ApoE−/− P. gingivalis-infected mice brains (24 weeks) provided evidence of BBB deterioration, and cerebral tissue damage (Singhrao et al., 2017). The BBB breach may have resulted from acute phase inflammation in the form of oxidative stress and P. gingivalis protease (gingipains) activity in these mice (Rokad et al., 2017; Singhrao et al., 2017). Gingipains have been shown to degrade collagens (Bedi and Williams, 1994; Zhou and Windsor, 2006), and cell-cell adhesion molecules (cadherins and integrins) contributing to endothelial and epithelial barrier disruption (Katz et al., 2000; Hintermann et al., 2002; Sheets et al., 2005).
Disruption of the BBB is an early feature of AD (van de Haar et al., 2016), and P. gingivalis may be one of the many factors contributing to this adverse outcome. The weakened BBB may enable the cerebral entry of other pathogens (viral, fungal, and bacterial origin), that are detected in AD brains. The leaky BBB might also favor the entry of other environmental toxins (e.g., pesticides, pollution, aluminum, or heavy metals) that have been associated with Aβ fibrils in AD (Carter, 2017). In addition, the P. gingivalis interactome was highly enriched in cerebral arterial genes, which further supports the correlation with BBB damage. Although P. gingivalis LPS is able to provoke Aβ deposition (Wu et al., 2017), the bacterial interactome genes overlapping with the AD GWASdb genes were not significantly enriched in the KEGG AD pathway, suggesting that the BBB-related effects of P. gingivalis may be of greater relevance to its developing pathology. In the periphery, vessel damage likely by gingipains may also allow bacteria to enter the circulatory system and cause transient bacteraemia that contribute to atherosclerosis (Bahrani-Mougeot et al., 2008; Lockhart et al., 2008; Singhrao et al., 2017) and possibly insulin resistance. The endothelial vessel barrier is disrupted in diabetes and in atherosclerosis (Chistiakov et al., 2015; Dong et al., 2016) showing common defects with AD.
There are several general caveats to this type of analysis. For example, the susceptibility gene/interactome overlaps deal with gene symbols rather than with specific polymorphisms, and there have been no studies linking host genetic polymorphisms to specific components of the P. gingivalis life cycle. The effects of the pathogen may also be strain-specific and some strains may be more virulent. Within any large interactome, effects in relation to the host may be null, deleterious or even beneficial. The statistical analyses similarly rely on the degree of overlap between two sets of gene symbols, with no indication of physiological weight or direction. However, any of the individual gene effects noted accordingly referenced on the following website (http://www.polygenicpathways.co.uk/pgingivalis.htm). The pathway analyses similarly depend upon those made available by particular websites and pathways that are more relevant may exist but remain inaccessible to the wider public.
The host genes employed by P. gingivalis during its life cycle, and those reacting to infection by the pathogen-host interaction are enriched in GWASdb genes for several diseases where the pathogen is suspected to play a contributory role. These notably include AD, T2DM, and atherosclerosis. This was confirmed for associated genes in the more stringent NHGRI-EBI GWAS database. The genes misregulated in periodontal tissue or by P. gingivalis or its components in macrophages also relate to those misregulated in AD and atherosclerosis. It may be plausible to suggest that the major effects of P. gingivalis relate to its ability to disrupt barrier function and this could play a key role in its downstream pathological events. Disrupted BBB function caused by P. gingivalis may also be relevant to the many other pathogens that have been associated with AD and related diseases.
From a genetic standpoint, it is increasingly clear that disease susceptibility genes relate not only to human physiology, but also to that of the pathogens implicated in the same disease. This suggests that pathogens and genes condition each other's effects. It is not yet clear how or whether polymorphisms in these susceptibility genes influence the disease promoting effects of this pathogen, and, in general, there has been little work in this area. It is also likely that such gene/pathogen interactions could determine the extent to which P. gingivalis can influence the acute or chronic aspects of periodontitis or other components of the oral microbiome. From a medical standpoint it is however evident that the prevention of periodontal disease or strategies directed at keystone pathogens such as P. gingivalis could have a major effect on the incidence and progression of AD, cardiovascular diseases and type 2 diabetes, and possibly many other disorders.
This bioinformatics analysis supports the many documented relationships between P. gingivalis infection and AD or its comorbid conditions, T2DM and atherosclerosis. The key effects of the pathogen may relate to barrier disruption and inflammatory processes. Further studies should include animal models to determine whether this pathogen can damage and breach the BBB and whether it can access the brain and promote the hallmark pathologies of AD, including Aβ deposition, tau phosphorylation, inflammation and neuronal death in relevant areas. It is also likely that targeting this and other periodontal pathogens could be of benefit to a variety of human diseases.
CC: Manually curated the P. gingivalis/host interactome, created the figures and tables. CC, JF, and SS contributed to the many draft versions of all sections of the manuscript. SC provided critical feedback and funding.
This work was supported by the University of Central Lancashire, UK (JF, SS).
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
JF and SS wish to thank the University of Central Lancashire, UK for their continued financial support.
Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., and Dewhirst, F. E. (2005). Defining the normal bacterial flora of the oral cavity. J. Clin. Microbiol. 43, 5721–5732. doi: 10.1128/JCM.43.11.5721-5732.2005
Al Jewair, T. S., Al Jasser, R., and Almas, K. (2015). Periodontitis and obstructive sleep apnea's bidirectional relationship: a systematic review and meta-analysis. Sleep Breath 19, 1111–1120. doi: 10.1007/s11325-015-1160-8
Amaral Cda, S., da Silva-Boghossian, C. M., Leão, A. T., and Colombo, A. P. (2011). Evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. J. Dent. 39, 729–738. doi: 10.1016/j.jdent.2011.08.004
Bahrani-Mougeot, F. K., Paster, B. J., Coleman, S., Ashar, J., Barbuto, S., and Lockhart, P. B. (2008). Diverse and novel oral bacterial species in blood following dental procedures. J. Clin. Microbiol. 46, 2129–2132. doi: 10.1128/JCM.02004-07
Bailey, M. T., Kinsey, S. G., Padgett, D. A., Sheridan, J. F., and Leblebicioglu, B. (2009). Social stress enhances IL-1beta and TNF-alpha production by Porphyromonas gingivalis lipopolysaccharide-stimulated CD11b+ cells. Physiol. Behav. 98, 351–358. doi: 10.1016/j.physbeh.2009.06.013
Bale, B. F., Doneen, A. L., and Vigerust, D. J. (2017). High-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. Postgrad. Med. J. 93, 215–220. doi: 10.1136/postgradmedj-2016-134279
Barbosa, G. M., Colombo, A. V., Rodrigues, P. H., and Simionato, M. R. (2015). Intraspecies variability affects heterotypic biofilms of Porphyromonas gingivalis and Prevotella intermedia: evidences of strain-dependence biofilm modulation by physical contact and by released soluble factors. PLoS ONE 10:e0138687. doi: 10.1371/journal.pone.0138687
Belanger, M., Reyes, L., von Deneen, K., Reinhard, M. K., Progulske-Fox, A., and Brown, M. B. (2008). Colonization of maternal and fetal tissues by Porphyromonas gingivalis is strain-dependent in a rodent animal model. Am. J. Obstet. Gynecol. 199, 86–87. doi: 10.1016/j.ajog.2007.11.067
Blalock, E. M., Geddes, J. W., Chen, K. C., Porter, N. M., Markesbery, W. R., and Landfield, P. W. (2004). Incipient Alzheimer's disease: microarray correlation analyses reveal major transcriptional and tumor suppressor responses. Proc. Natl. Acad. Sci. U.S.A. 101, 2173–2178. doi: 10.1073/pnas.0308512100
Bonfim Mde, L., Mattos, F. F., Ferreira e Ferreira, E., Campos, A. C., and Vargas, A. M. (2013). Social determinants of health and periodontal disease in Brazilian adults: a cross-sectional study. BMC Oral Health 13:22. doi: 10.1186/1472-6831-13-22
Bourgade, K., Garneau, H., Giroux, G., Le Page, A. Y., Bocti, C., Dupuis, G., et al. (2015). β-Amyloid peptides display protective activity against the human Alzheimer's disease-associated herpes simplex virus-1. Biogerontology 16, 85–98. doi: 10.1007/s10522-014-9538-8
Bramanti, E., Bramanti, A., Matacena, G., Bramanti, P., Rizzi, A., and Cicciu, M. (2015). Clinical evaluation of the oral health status in vascular-type dementia patients. A case-control study. Minerva Stomatol. 64, 167–175.
Budzynski, J., Wiśniewska, J., Ciecierski, M., and Kedzia, A. (2016). Association between bacterial infection and peripheral vascular disease: a review. Int. J. Angiol. 25, 3–13. doi: 10.1055/s-0035-1547385
Carter, C. (2011). Alzheimer's disease: APP, gamma secretase, APOE, CLU, CR1, PICALM, ABCA7, BIN1, CD2AP, CD33, EPHA1, and MS4A2, and their relationships with herpes simplex, C. pneumoniae, other suspect pathogens, and the immune system. Int. J. Alzheimers Dis. 2011:501862. doi: 10.4061/2011/501862
Carter, C. J. (2013a). Susceptibility genes are enriched in those of the herpes simplex virus 1/host interactome in psychiatric and neurological disorders. Pathog. Dis. 69, 240–261. doi: 10.1111/2049-632X.12077
Carter, C. J. (2013b). Toxoplasmosis and polygenic disease susceptibility genes: extensive Toxoplasma gondii host/pathogen interactome enrichment in nine Psychiatric or neurological disorders. J. Pathog. 2013:29. doi: 10.1155/2013/965046
Carter, C. J. (2017). Genetic, transcriptome, proteomic and epidemiological evidence for blood brain barrier disruption and polymicrobial brain invasion as determinant factors in Alzheimers disease. J. Alzheimers Dis. Rep. 1, 125–157. doi: 10.3233/ADR-170017
Castrillon, C. A., Hincapie, J. P., Yepes, F. L., Roldan, N., Moreno, S. M., Contreras, A., et al. (2015). Occurrence of red complex microorganisms and Aggregatibacter actinomycetemcomitans in patients with diabetes. J. Investig. Clin. Dent. 6, 25–31. doi: 10.1111/jicd.12051
Chen, C. K., Wu, Y. T., and Chang, Y. C. (2017). Association between chronic periodontitis and the risk of Alzheimer's disease: a retrospective, population-based, matched-cohort study. Alzheimers Res. Ther. 9:56. doi: 10.1186/s13195-017-0282-6
Chen, T., Yu, W. H., Izard, J., Baranova, O. V., Lakshmanan, A., and Dewhirst, F. E. (2010). The human oral microbiome database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database 2010:baq013. doi: 10.1093/database/baq013
Demmer, R. T., Jacobs, D. R., Singh, R., Zuk, A., Rosenbaum, M., Papapanou, P. N., et al. (2015). Periodontal bacteria and prediabetes prevalence in ORIGINS: the oral infections, glucose intolerance, and insulin resistance study. J. Dent. Res. 94, 201S−211S. doi: 10.1177/0022034515590369
Dong, Y., Fernandes, C., Liu, Y., Wu, Y., Wu, H., Brophy, M. L., et al. (2016). Role of endoplasmic reticulum stress signalling in diabetic endothelial dysfunction and atherosclerosis. Diab. Vasc. Dis. Res. 14, 14–23. doi: 10.1177/1479164116666762
Emery, D. C., Shoemark, D. K., Batstone, T. E., Waterfall, C. M., Coghill, J. A., Cerajewska, T. L., et al. (2017). 16S rRNA next generation sequencing analysis shows bacteria in Alzheimer's post-mortem brain. Front. Aging Neurosci. 9:195. doi: 10.3389/fnagi.2017.00195
Gaydos, J., McNally, A., Guo, R., Vandivier, R. W., Simonian, P. L., and Burnham, E. L. (2016). Alcohol abuse and smoking alter inflammatory mediator production by pulmonary and systemic immune cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 3, L507–L518. doi: 10.1152/ajplung.00242.2015
Geraldino-Pardilla, L., Giles, J. T., Sokolove, J., Zartoshti, A., Robinson, W. H., Budoff, M., et al. (2017). Association of anti–citrullinated peptide antibodies with coronary artery calcification in rheumatoid arthritis. Arthritis Care Res. 69, 1276–1281. doi: 10.1002/acr.23106
Guo, X., Wang, Y., Wang, C., and Chen, J. (2015). Identification of several hub-genes associated with periodontitis using integrated microarray analysis. Mol. Med. Rep. 11, 2541–2547. doi: 10.3892/mmr.2014.3031
Hajishengallis, G., Krauss, J. L., Liang, S., McIntosh, M. L., and Lambris, J. D. (2012). Pathogenic microbes and community service through manipulation of innate immunity. Adv. Exp. Med. Biol. 946, 69–85. doi: 10.1007/978-1-4614-0106-3_5
Hanaoka, Y., Soejima, H., Yasuda, O., Nakayama, H., Nagata, M., Matsuo, K., et al. (2013). Level of serum antibody against a periodontal pathogen is associated with atherosclerosis and hypertension. Hypertens. Res. 36, 829–833. doi: 10.1038/hr.2013.46
Hansen, G. M., Egeberg, A., Holmstrup, P., and Hansen, P. R. (2016). Relation of periodontitis to risk of cardiovascular and all-cause mortality (from a Danish Nationwide Cohort Study). Am. J. Cardiol. 118, 489–493. doi: 10.1016/j.amjcard.2016.05.036
Harding, A., Robinson, S., Crean, S. K., and Singhrao, S. (2017). Can better management of periodontal disease delay the onset and progression of Alzheimer's disease? J. Alzheimers Dis. 58, 337–348. doi: 10.3233/JAD-170046
Hintermann, E., Haake, S. K., Christen, U., Sharabi, A., and Quaranta, V. (2002). Discrete proteolysis of focal contact and adherens junction components in Porphyromonas gingivalis-infected oral keratinocytes: a strategy for cell adhesion and migration disabling. Infect. Immun. 70, 5846–5856. doi: 10.1128/IAI.70.10.5846-5856.2002
Hofman, A., Ott, A., Breteler, M. M., Bots, M. L., Slooter, A. J., Van Harskamp, F., et al. (1997). Atherosclerosis, apolipoprotein E, and prevalence of dementia and Alzheimer's disease in the Rotterdam Study. Lancet 349, 151–154. doi: 10.1016/S0140-6736(96)09328-2
Holmlund, A., Lampa, E., and Lind, L. (2017). Oral health and cardiovascular disease risk in a cohort of periodontitis patients. Atherosclerosis 262, 101–106. doi: 10.1016/j.atherosclerosis.2017.05.009
Hsu, C. C., Hsu, Y. C., Chen, H. J., Lin, C. C., Chang, K. H., Lee, C. Y., et al. (2015). Association of periodontitis and subsequent depression: a nationwide population-based study. Medicine 94:e2347. doi: 10.1097/MD.0000000000002347
Ide, M., Harris, M., Stevens, A., Sussams, R., Hopkins, V., Culliford, D., et al. (2016). Periodontitis and cognitive decline in Alzheimer's disease. PLoS ONE 11:e0151081. doi: 10.1371/journal.pone.0151081
Imamura, K., Kokubu, E., Kita, D., Ota, K., Ishihara, K., and Saito, A. (2015). Cigarette smoke condensate modulates migration of human gingival epithelial cells and their interactions with Porphyromonas gingivalis. J. Periodont. Res. 50, 411–421. doi: 10.1111/jre.12222
Kamer, A. R., Janal, M. N., and de Leon, M. (2015). Letter to the editor regarding: summary of the evidence on modifiable risk factors for cognitive decline and dementia: a population-based perspective. Alzheimers Dement. 1, 385–386. doi: 10.1016/j.dadm.2015.08.003
Kamma, J. J., Lygidakis, N. A., and Nakou, M. (1998). Subgingival microflora and treatment in prepubertal periodontitis associated with chronic idiopathic neutropenia. J. Clin. Periodontol. 25, 759–765. doi: 10.1111/j.1600-051X.1998.tb02518.x
Katz, J., Sambandam, V., Wu, J. H., Michalek, S. M., and Balkovetz, D. F. (2000). Characterization of Porphyromonas gingivalis-induced degradation of epithelial cell junctional complexes. Infect. Immun. 68, 1441–1449. doi: 10.1128/IAI.68.3.1441-1449.2000
Kibbe, W. A., Arze, C., Felix, V., Mitraka, E., Bolton, E., Fu, G., et al. (2015). Disease ontology 2015 update: an expanded and updated database of human diseases for linking biomedical knowledge through disease data. Nucleic Acids Res. 43, D1071–D1078. doi: 10.1093/nar/gku1011
Kumar, A., Kardkal, A., Debnath, S., and Lakshminarayan, J. (2015). Association of periodontal health indicators and major depressive disorder in hospital outpatients. J. Indian Soc. Periodontol. 19, 507–511. doi: 10.4103/0972-124X.167161
Kumar, D. K., Choi, S. H., Washicosky, K. J., Eimer, W. A., Tucker, S., Ghofrani, J., et al. (2016). Amyloid-β peptide protects against microbial infection in mouse and worm models of Alzheimer's disease. Sci. Transl. Med 8, 340ra372–340ra372.
Leira, Y., Domínguez, C., Seoane, J., Seoane-Romero, J., Pías-Peleteiro, J. M., Takkouche, B., et al. (2017). Is periodontal disease associated with Alzheimer's disease? A systematic review with meta-analysis. Neuroepidemiology 48, 21–31. doi: 10.1159/000458411
Li, H., Yang, H., Ding, Y., Aprecio, R., Zhang, W., Wang, Q., et al. (2013). Experimental periodontitis induced by Porphyromonas gingivalis does not alter the onset or severity of diabetes in mice. J. Periodont. Res. 48, 582–590. doi: 10.1111/jre.12041
Li, M. J., Wang, P., Liu, X., Lim, E. L., Wang, Z., Yeager, M., et al. (2012). GWASdb: a database for human genetic variants identified by genome-wide association studies. Nucleic Acids Res. 40, D1047–D1054. doi: 10.1093/nar/gkr1182
Lockhart, P. B., Brennan, M. T., Sasser, H. C., Fox, P. C., Paster, B. J., and Bahrani-Mougeot, F. K. (2008). Bacteremia associated with tooth brushing and dental extraction. Circulation 117, 3118–3125. doi: 10.1161/CIRCULATIONAHA.107.758524
Macarthur, J., Bowler, E., Cerezo, M., Gil, L., Hall, P., Hastings, E., et al. (2017). The new NHGRI-EBI catalog of published genome-wide association studies (GWAS Catalog). Nucleic Acids Res. 45, D896–D901. doi: 10.1093/nar/gkw1133
Mahanonda, R., Sa-Ard-Iam, N., Eksomtramate, M., Rerkyen, P., Phairat, B., Schaecher, K. E., et al. (2009). Cigarette smoke extract modulates human beta-defensin-2 and interleukin-8 expression in human gingival epithelial cells. J. Periodont. Res. 44, 557–564. doi: 10.1111/j.1600-0765.2008.01153.x
Matsushita, K., Hamaguchi, M., Hashimoto, M., Yamazaki, M., Yamazaki, T., Asai, K., et al. (2015). The novel association between red complex of oral microbe and body mass index in healthy Japanese: a population based cross-sectional study. J. Clin. Biochem. Nutr. 57, 135–139. doi: 10.3164/jcbn.15-19
McCreadie, R. G., Stevens, H., Henderson, J., Hall, D., McCaul, R., Filik, R., et al. (2004). The dental health of people with schizophrenia. Acta Psychiatr. Scand. 110, 306–310. doi: 10.1111/j.1600-0447.2004.00373.x
Miklossy, J., and McGeer, P. L. (2016). Common mechanisms involved in Alzheimer's disease and type 2 diabetes: a key role of chronic bacterial infection and inflammation. Aging 8, 575–588. doi: 10.18632/aging.100921
Mougeot, J. C., Stevens, C. B., Paster, B. J., Brennan, M. T., Lockhart, P. B., and Mougeot, F. K. (2017). Porphyromonas gingivalis is the most abundant species detected in coronary and femoral arteries. J. Oral Microbiol. 9:1281562. doi: 10.1080/20002297.2017.1281562
Naorungroj, S., Slade, G. D., Beck, J. D., Mosley, T. H., Gottesman, R. F., Alonso, A., et al. (2013). Cognitive decline and oral health in middle-aged adults in the ARIC study. J. Dent. Res. 92, 795–801. doi: 10.1177/0022034513497960
Navratil, V., de Chassey, B., Combe, C. R., and Lotteau, V. (2011). When the human viral infectome and diseasome networks collide: towards a systems biology platform for the aetiology of human diseases. BMC Syst. Biol. 5:13. doi: 10.1186/1752-0509-5-13
Noble, J. M., Borrell, L. N., Papapanou, P. N., Elkind, M. S., Scarmeas, N., and Wright, C. B. (2009). Periodontitis is associated with cognitive impairment among older adults: analysis of NHANES-III. J. Neurol. Neurosurg. Psychiatr. 80, 1206–1211. doi: 10.1136/jnnp.2009.174029
Oliveros (2007). VENNY An Interactive Tool For Comparing lists using Venn Diagrams. Available online at: http://bioinfogp.cnb.csic.es/tools/venny/
Olsen, I., Lambris, J. D., and Hajishengalis, G. (2017). Porphyromonas gingivalis disturbs host-commensal homeostasis by changing complement function. J. Oral Microbiol. 9:1340085. doi: 10.1080/20002297.2017.1340085
Olsen, I., Taubman, M. A., and Singhrao, S. K. (2016). Porphyromonas gingivalis suppresses adaptive immunity in periodontitis, atherosclerosis, and Alzheimer's disease. J. Oral Microbiol. 8:33029. doi: 10.3402/jom.v8.33029
Papaspyridonos, M., Smith, A., Burnand, K. G., Taylor, P., Padayachee, S., Suckling, K. E., et al. (2006). Novel candidate genes in unstable areas of human atherosclerotic plaques. Arterioscler. Thromb. Vasc. Biol. 26, 1837–1844. doi: 10.1161/01.ATV.0000229695.68416.76
Pataro, A. L., Cortelli, S. C., Abreu, M. H., Cortelli, J. R., Franco, G. C., Aquino, D. R., et al. (2016). Frequency of periodontal pathogens and Helicobacter pylori in the mouths and stomachs of obese individuals submitted to bariatric surgery: a cross-sectional study. J. Appl. Oral Sci. 24, 229–238. doi: 10.1590/1678-775720150534
Pisa, D., Alonso, R., Fernández-Fernández, A. M., Rábano, A., and Carrasco, L. (2017). Polymicrobial infections in brain tissue from Alzheimer's disease patients. Sci. Rep. 7:5559. doi: 10.1038/s41598-017-05903-y
Poole, S., Singhrao, S. K., Chukkapalli, S., Rivera, M., Velsko, I., Kesavalu, L., et al. (2015). Active invasion of Porphyromonas gingivalis and infection-induced complement activation in ApoE−/− mice brains. J. Alzheimers Dis. 43, 67–80. doi: 10.3233/JAD-140315
Poole, S., Singhrao, S. K., Kesavalu, L., Curtis, M. A., and Crean, S. (2013). Determining the presence of periodontopathic virulence factors in short-term postmortem Alzheimer's disease brain tissue. J. Alzheimers Dis. 36, 665–677. doi: 10.3233/JAD-121918
Riviere, G. R., Riviere, K. H., and Smith, K. S. (2002). Molecular and immunological evidence of oral Treponema in the human brain and their association with Alzheimer's disease. Oral Microbiol. Immunol. 17, 113–118. doi: 10.1046/j.0902-0055.2001.00100.x
Roher, A. E., Esh, C., Kokjohn, T. A., Kalback, W., Luehrs, D. C., Seward, J. D., et al. (2003). Circle of willis atherosclerosis is a risk factor for sporadic Alzheimer's disease. Arterioscler. Thromb. Vasc. 23, 2055–2062. doi: 10.1161/01.ATV.0000095973.42032.44
Rokad, F., Moseley, R. S., Hardy, R., Chukkapalli, S., Crean, S., Singhrao, S., et al. (2017). Cerebral oxidative stress and microvasculature defects in TNF-α expressing transgenic and Porphyromonas gingivalis-infected ApoE−/− mice. J. Alzheimers. Dis. 60, 359–369. doi: 10.3233/JAD-170304
Rozenblatt-Rosen, O., Deo, R. C., Padi, M., Adelmant, G., Calderwood, M. A., Rolland, T., et al. (2012). Interpreting cancer genomes using systematic host perturbations by tumour virus proteins. Nature 487, 491–495. doi: 10.1038/nature11288
Sender-Janeczek, A., and Zietek, M. (2016). The distribution of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans in patients with alcoholic disease: a pilot study. Adv. Clin. Exp. Med. 5, 243–248. doi: 10.17219/acem/61079
Sheets, S. M., Potempa, J., Travis, J., Casiano, C. A., and Fletcher, H. M. (2005). Gingipains from Porphyromonas gingivalis W83 induce cell adhesion molecule cleavage and apoptosis in endothelial cells. Infect. Immun. 73, 1543–1552. doi: 10.1128/IAI.73.3.1543-1552.2005
Shin, H. S., Shin, M. S., Ahn, Y. B., Choi, B. Y., Nam, J. H., and Kim, H. D. (2016). Periodontitis is associated with cognitive impairment in elderly koreans: results from the yangpyeong cohort study. J. Am. Geriatr. Soc. 64, 162–167. doi: 10.1111/jgs.13781
Silvestre, F. J., Lauritano, D., Carinci, F., Silvestre-Rangil, J., Martinez-Herrera, M., and Del Olmo, A. (2017). Neuroinflammation, Alzheimers disease and periodontal disease: is there an association between the two processes? J. Biol. Regul. Homeost. Agents 31, 189–196.
Singh, A., Rouxel, P., Watt, R. G., and Tsakos, G. (2013). Social inequalities in clustering of oral health related behaviors in a national sample of British adults. Prev. Med. 57, 102–106. doi: 10.1016/j.ypmed.2013.04.018
Singhrao, S. K., Chukkapalli, S., Poole, S., Velsko, I., Crean, S. J., and Kesavalu, L. (2017). Chronic Porphyromonas gingivalis infection accelerates the occurrence of age-related granules in ApoE(–) (/) (–) mice brains. J. Oral Microbiol. 9:1270602. doi: 10.1080/20002297.2016.1270602
Singhrao, S. K., Harding, A., Poole, S., Kesavalu, L., and Crean, S. (2015). Porphyromonas gingivalis periodontal infection and its putative links with Alzheimers disease. Mediators Inflamm. 2015:137357.
Socransky, S. S., Haffajee, A. D., Cugini, M. A., Smith, C., and Kent, R. L. (1998). Microbial complexes in subgingival plaque. J. Clin. Periodontol. 25, 134–144. doi: 10.1111/j.1600-051X.1998.tb02419.x
Sokolove, J., Sharpe, O., Brennan, M. J., Lahey, L. J., Kao, A. H., Krishnan, E., et al. (2013). Citrullination within the atherosclerotic plaque: a potential target for the anti-citrullinated protein antibody response in rheumatoid arthritis. Arthritis Rheum. 65, 1719–1724. doi: 10.1002/art.37961
Soscia, S. J., Kirby, J. E., Washicosky, K. J., Tucker, S. M., Ingelsson, M., Hyman, B., et al. (2010). The Alzheimer's disease-associated amyloid β-protein is an antimicrobial peptide. PLoS ONE 5:e9505. doi: 10.1371/journal.pone.0009505
Sparks Stein, P., Steffen, M. J., Smith, C., Jicha, G., Ebersole, J. L., Abner, E., et al. (2012). Serum antibodies to periodontal pathogens are a risk factor for Alzheimer's disease. Alzheimers. Dement. 8, 196–203. doi: 10.1016/j.jalz.2011.04.006
Sugiyama, S., Takahashi, S.-S., Tokutomi, F.-A., Yoshida, A., Kobayashi, K., Yoshino, F., et al. (2012). Gingival vascular functions are altered in type 2 diabetes mellitus model and/or periodontitis model. J. Clin. Biochem. Nutr. 51, 108–113. doi: 10.3164/jcbn.11-103
Syrjälä, A. M., Ylöstalo, P., Ruoppi, P., Komulainen, K., Hartikainen, S., Sulkava, R., et al. (2012). Dementia and oral health among subjects aged 75 years or older. Gerodontology 29, 36–42. doi: 10.1111/j.1741-2358.2010.00396.x
Trombelli, L., Scapoli, C., Orlandini, E., Tosi, M., Bottega, S., and Tatakis, D. N. (2004). Modulation of clinical expression of plaque-induced gingivitis. III. Response of ‘high responders’ and ‘low responders’ to therapy. J. Clin. Periodontol. 31, 253–259.
van de Haar, H. J., Burgmans, S., Jansen, J. F., Van Osch, M. J., Van Buchem, M. A., Muller, M., et al. (2016). Blood-brain barrier leakage in patients with early Alzheimer disease. Radiology 281, 527–535. doi: 10.1148/radiol.2016152244
Velsko, I. M., Chukkapalli, S. S., Rivera, M. F., Lee, J. Y., Chen, H., Zheng, D., et al. (2014). Active invasion of oral and aortic tissues by Porphyromonas gingivalis in mice causally links periodontitis and atherosclerosis. PLoS ONE 9:e97811. doi: 10.1371/journal.pone.0097811
Walter, C., Zahlten, J., Schmeck, B., Schaudinn, C., Hippenstiel, S., Frisch, E., et al. (2004). Porphyromonas gingivalis strain-dependent activation of human endothelial cells. Infect. Immun. 72, 5910–5918. doi: 10.1128/IAI.72.10.5910-5918.2004
White, M. R., Kandel, R., Tripathi, S., Condon, D., Qi, L., Taubenberger, J., et al. (2014). Alzheimer's associated β-amyloid protein inhibits influenza A virus and modulates viral interactions with phagocytes. PLoS ONE 9:e101364. doi: 10.1371/journal.pone.0101364
Wilensky, A., Polak, D., Awawdi, S., Halabi, A., Shapira, L., and Houri-Haddad, Y. (2009). Strain-dependent activation of the mouse immune response is correlated with Porphyromonas gingivalis-induced experimental periodontitis. J. Clin. Periodontol. 36, 915–921. doi: 10.1111/j.1600-051X.2009.01464.x
Wood, N., and Johnson, R. B. (2004). The relationship between tomato intake and congestive heart failure risk in periodontitis subjects. J. Clin. Periodontol. 31, 574–580. doi: 10.1111/j.1600-051X.2004.00531.x
Wu, Z., Ni, J., Liu, Y., Teeling, J. L., Takayama, F., Collcutt, A., et al. (2017). Cathepsin B plays a critical role in inducing Alzheimer's disease-like phenotypes following chronic systemic exposure to lipopolysaccharide from Porphyromonas gingivalis in mice. Brain Behav. Immun. 65, 350–361. doi: 10.1016/j.bbi.2017.06.002
Zhou, J., and Windsor, L. J. (2006). Porphyromonas gingivalis affects host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases. J. Periodont. Res. 41, 47–54. doi: 10.1111/j.1600-0765.2005.00835.x
Keywords: Alzheimer's disease, cardiovascular, diabetes, interactome, Porphyromonas gingivalis
Citation: Carter CJ, France J, Crean S and Singhrao SK (2017) The Porphyromonas gingivalis/Host Interactome Shows Enrichment in GWASdb Genes Related to Alzheimer's Disease, Diabetes and Cardiovascular Diseases. Front. Aging Neurosci. 9:408. doi: 10.3389/fnagi.2017.00408
Received: 19 September 2017; Accepted: 27 November 2017;
Published: 12 December 2017.
Edited by:Aurel Popa-Wagner, University of Rostock, Germany
Reviewed by:Daniel Ortuño-Sahagún, University of Guadalajara, Mexico
Chi-Hua Chen, University of California, San Diego, United States
Copyright © 2017 Carter, France, Crean and Singhrao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sim K. Singhrao, email@example.com