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PERSPECTIVE article

Front. Cell. Neurosci., 10 January 2013
Sec. Cellular Neurophysiology

GABA-B receptors in the PNS have a role in Schwann cells differentiation?


Patrizia Procacci Patrizia Procacci1Marinella Ballabio Marinella Ballabio2Luca F. Castelnovo Luca F. Castelnovo2Cristina Mantovani Cristina Mantovani2Valerio Magnaghi* Valerio Magnaghi2*
  • 1Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy
  • 2Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy

γ-aminobutyric acid type B (GABA-B) receptor mediates the inhibitory transmission of γ-aminobutyric acid in the mammalian nervous system, being present in neurons and also in glial cells. Recently the presence of GABA-B has been demonstrated in Schwann cells (SC) suggesting its contribution in regulating the cell fate, maturation, and plasticity. Here, we further support the functional presence of GABA-B receptor in SC plasma membrane. By confocal microscopy immunofluorescence we provide evidences that GABA-B localization on the cell elongated processes correlates with the morphological changes occurring in the differentiated SC. In vivo most of the GABA-B receptors seem to be present in non-myelinating SC, which are committed to ensheath the nociceptive fibers. Therefore, we argue that GABA-B receptors do not control exclusively the in vivo differentiation yielding the myelinating SC, but are also fundamental in regulating the SC plasticity versus the non-myelinating state. Data from the literature and our recent findings corroborate the role of the GABAergic system and GABA-B receptors in the peripheral nervous system, opening new perspectives on the mechanisms controlling the differentiation of SC.

Introduction

Unlike neurons in the central nervous system (CNS), the peripheral nervous system (PNS) has the capacity to re-growth after a damage. This regenerative capacity mainly rests on the plasticity of Schwann cells (SC) and on their ability to switch between differentiation states (Jessen and Mirsky, 2005). SC, that normally produce an insulating myelin sheath around the axons, stimulate the survival and re-growth of injured nerves. Regenerative de- and re-differentiation as well as developmental differentiation, are tightly controlled by a balance of positive/negative regulators of SC maturation (Jessen and Mirsky, 2005, 2008). The comprehension of the mechanisms that contribute to SC plasticity are important not only to understand basic principles regulating SC biology and neuronal regeneration, but also because some of the molecules involved may act as modulators of peripheral neuropathies and associated neuropathic pain (Jessen and Mirsky, 2008). In recent years, the increased understanding of the cellular mechanisms controlling neuron-glial crosstalk in the PNS has focused on classic neurotransmitters, such as adenosine triphosphate (ATP) or γ-aminobutyric acid (GABA) (Fields and Stevens-Graham, 2002; Magnaghi et al., 2009). Here we survey the latest findings on the involvement of GABA system in SC, particularly via type B (GABA-B) receptor, assessing its potential as novel tool to regulate SC fate and plasticity.

GABA-B Receptor in the PNS

GABA-B Receptor

In the mammalian nervous system the GABA-B receptor is one of the native targets of GABA, the main inhibitory neurotransmitter (Bowery et al., 2002; Olsen and Sieghart, 2008). GABA-B is a distinct, baclofen-sensitive, metabotropic receptor, member of the seven transmembrane G-protein-coupled receptor superfamily. The intracellular signaling involves the activation of the adenylate cyclase second messenger systems and/or calcium and potassium channels activities (Bowery et al., 2002). GABA-B receptor is a heterodimer composed of two subunits, respectively GABA-B1 that is responsible for the ligand binding (Kaupmann et al., 1997, 1998; Jones et al., 1998; White et al., 1998; Kuner et al., 1999; Pfaff et al., 1999), and GABA-B2, which is necessary for the trafficking of the heterodimer to the cell surface and for signal transduction (Ng et al., 1999).

GABA-B Receptor in the PNS

The presence of GABA and its receptors in peripheral nerves date back to the 1980's (Brown and Marsh, 1978; Brown et al., 1979; Jessen et al., 1979; Morris et al., 1983; Olsen et al., 1984; Bhisitkul et al., 1987), but only recent findings yielded by reverse transcriptase-polymerase chain reaction, western blot, and immunohistochemistry, have clearly stated that GABA-B receptors are present in the PNS (Magnaghi et al., 2004, 2006; Magnaghi, 2007).

GABA-B Receptor in the PNS of Knockout Mice

The absence of GABA-B1 receptors has been confirmed in different nervous and non-nervous tissues of GABA-B1 knockout mice (Schuler et al., 2001). Hence, these mice are a good model to study the biochemical, morphological, and physiological consequences of the constitutive loss of GABA-B functions (Schuler et al., 2001). Recently, it has been shown that lack of GABA-B receptor activity in GABA-B1 knockout mice affects PNS myelination process, which in turn can contribute to the sensory phenotype observed (Magnaghi et al., 2008). Indeed, GABA-B1 deficient mice exhibited morphological and molecular changes in the peripheral myelin, including increases in the number of irregular fibers and in the expression levels of the myelin proteins, such as peripheral myelin protein 22 (PMP22) and myelin protein zero (P0). The density of small myelinated fibers and small neurons of the lumbar dorsal root ganglia (DRG) was raised in GABA-B1 deficient mice. These changes were also associated to altered gait behavior and pain perception, as well as with hyperalgesia to thermal stimuli (Magnaghi et al., 2008).

GABA-B Receptor in SC

In the PNS GABA-B receptors are mostly localized on the SC (Magnaghi et al., 2004; Magnaghi, 2007; Faroni et al., 2011). In these cells, GABA-B receptors proved to be negatively coupled to the adenylate cyclase system (Magnaghi et al., 2004), controlling SC proliferation and, overall, the myelin gene expression, both in vitro and in vivo (Magnaghi et al., 2004, 2008). Indeed, the activation of GABA-B receptor with the specific agonist baclofen counteracted the forskolin-induced SC proliferation at 5 days in vitro, with a reduction of the percentage of BrdUrd immunopositive SC (Magnaghi et al., 2004). In addition, GABA-B receptor activation decreased the expression levels of some myelin proteins, like PMP22, P0, connexin 32, and the myelin-associated glycoprotein (MAG) (Magnaghi et al., 2004). Given that the enzyme adenylate cyclase is able to regulate SC proliferation (Lee et al., 1999; Mirsky and Jessen, 1999) and the expression of specific myelin proteins (Suter et al., 1994; Scherer et al., 1995; Lee et al., 1999), it was conceivable to hypothesise that the GABA-B-induced decrease in cAMP levels accounts for the changes in cell proliferation and myelin gene expression in SC.

However, the trafficking of the GABA-B receptor complex to the plasma membrane is also crucial for its functional activity (Bettler and Tiao, 2006). Recent studies evaluated the subcellular localization of GABA-B receptors in SC by protein fractioning and western blot analysis. Two bands of 130 kDa and 100 kDa, corresponding to the GABA-B 1a and 1b native isoforms respectively (Kaupmann et al., 1997; Ige et al., 2000), were found in the membrane-enriched fraction of SC. By contrast, very faint bands likely corresponding to the GABA-B1 in the secretory granules still persisted in the cytoplasmic fraction (Figure 1A). Accordingly, in SC treated with the enzyme chymotrypsin, which cleaves the peptidic bonds of proteins localized in the outer plasma membrane, the two bands corresponding to the GABA-B1 isoforms disappeared, whereas very weak bands still corresponding to the cytoplasmic fraction appeared (Figure 1B). A further evaluation of the GABA-B expression in SC membrane was done by immunofluorescence and confocal laser scanner microscopy (CLSM). f-actin is a protein producing a polymer that lies beneath the plasma membrane (Pollard and Borisy, 2003), and that is also present in lateral microvilli of SC (Trapp et al., 1989). As a valuable tool for f-actin labeling (Faulstich et al., 1983; Barber et al., 2004), the tetramethyl rhodamine isothiocyanate (TRITC)-fluorescent conjugate of phalloidin is useful to highlight the SC perimeter and shape (red in Figure 1C). GABA-B1 subunit was detected in SC cytoplasm and in cellular processes (green in Figure 1C), while GABA-B2 was found in SC body compartment (green in Figure 1C). Although GABA-B1 and GABA-B2 appeared generally spread throughout the SC body, the cytoplasmic presence was not unexpected because data from the literature indicates that GABA-B receptors may function as co-regulator of nuclear transcription factors such as ATF-4 or CREB-2 (Charles et al., 2003). However, merge images revealed that both GABA-B1 and GABA-B2 receptors were localized in the SC membranes, likely bounded to the f-actin filaments tracing the cells border (yellow in Figure 1C). By processing the y-axis projections of the confocal images of GABA-B/f-actin co-localizations, GABA-B1 and GABA-B2 resulted particularly enriched at the membrane surfaces ensheathing the cell body compartments (green in Figure 1D), although the GABA-B labeling was also present at the cell borders outlined by the f-actin cytoskeleton (yellow in Figure 1D). As expected, all these observations further confirmed the cell membrane localization of GABA-B receptors in SC.

FIGURE 1
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Figure 1. GABA-B receptor is localized in plasma membranes of rat SC. (A) SC cultures were grown in Dulbecco's modified Eagle's medium with 10% fetal calf serum and they were highly pure, as characterized with a specific protein marker S100. Western immunoblot was then performed on the different SC subcellular protein fractions (nuclear, cytosolic, and membrane-enriched) separated by ultracentrifugation at 100,000 g. The antibody anti-GABA-B1 [1:250 (Magnaghi et al., 2004)] recognized two bands of ~130 kDa and 100 kDa, corresponding to the 1a and 1b isoforms of native receptors in the membrane-enriched fraction of SC (Kaupmann et al., 1997; Ige et al., 2000). GABA-B1 was absent in the nuclear-enriched fraction, whereas two faint bands, likely corresponding to the GABA-B1 in the secretory granules, still appeared in the cytosolic-enriched fraction of SC. Equal loading was evaluated by western blot against alpha-tubulin. (B) GABA-B1 immunoblot of chymotrypsin-treated SC (+) showed very weak bands likely corresponding to the residual receptors in the cytoplasmic fraction, while two GABA-B1 bands were present in the whole protein extract from untreated SC (−). Equal protein loading was evaluated by immunoblot of alpha-tubulin. (C) Immunofluorescence and CLSM. The cellular cytoskeleton was revealed by phalloidin-TRITC staining of f-actin (1:250, in red). GABA-B receptor detection was done with Alexa-488 (in green). Merge images of GABA-B1 and GABA-B2 (in yellow) show that both receptor subunits are expressed throughout the SC and co-localize with f-actin at the cell border surface (arrows). (D) Progressive y-axis projections of SC merge images revealing that both GABA-B1 and GABA-B2 receptors were present at cell membrane surfaces (arrows) and ensheath the cell body compartments. Negative GABA-B controls for specificity showed only phalloidin-TRITC immunostaining (data not shown). Scale bars 20 μm.

Does GABA-B have a Role in SC Differentiation?

SC committed in the process of myelination differentiate in vivo in response to contact with axons (Aguayo et al., 1976; Weinberg and Spencer, 1976). In vitro the same phenomenon of differentiation needs the presence of serum plus agents rising cAMP, like forskolin or 8Br-cAMP (Sobue et al., 1986; Morgan et al., 1991). In this context, agents modulating GABA-B receptors and in turn cAMP levels may thus participate in the control of SC differentiation and myelination processes.

The rearrangement of actin cytoskeleton is critical for SC differentiation and myelination (Fernandez-Valle et al., 1997; Gatto et al., 2003). During differentiation, SC change from a flat shape into a spindle-shaped phenotype; these cells present a reorganization in f-actin cytoskeleton and the appearance of bundles of stress fibers (Li et al., 2003). In such a way, the SC that assume the bipolar-shaped morphology resemble to the cells starting the myelination process in vivo.

Data collected in our laboratory, by means of co-localization studies (Bolte and Cordelieres, 2006), evidenced that the forskolin-induced SC differentiation correlates with a redistribution of GABA-B1 and GABA-B2 receptors on the SC surface. Indeed, in the undifferentiated SC (Figure 2A), the GABA-B1 receptor was localized on the protruding distal tips (arrows in Figure 2A magnification). When the SC differentiation starts, with changes in f-actin distribution and morphology (Figure 2B), GABA-B1 localized in the elongated regions, where the SC assumed the typical spindle-shaped morphology (arrows in Figure 2B magnification). In these selected regions the co-localization of f-actin and GABA-B1 receptors was 94.1 ± 6.7%. At the same time, in undifferentiated SC GABA-B2 showed a spread distribution (white in Figure 2C magnification), which turned to be localized in specific sites when, the SC differentiated and became elongated (Figure 2D). For instance, in these sites the f-actin boundaries were strictly assembled, SC cytoplasm extruded and the GABA-B2 receptor immunopositivity was very bright (arrows in Figure 2D magnification); the co-localization of f-actin and GABA-B2 receptors was 86.3 ± 9.7%.

FIGURE 2
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Figure 2. GABA-B receptor localization changes in differentiated SC in vitro and in vivo. Forskolin treatment (100 μM for 8 days)-induced strong f-actin cytoskeleton-based change in SC. Indeed, control SC (no forskolin) exhibited a polymerized f-actin wreath-like structure (A,C left panels in red), while forskolin treatment prompted the cells to present a stereotypical bipolar spindle-shape and elongated processes (B,D left panels in red). (A) and (B) Immunoflorescence for GABA-B1, (C) and (D) Immunoflorescence for GABA-B2, in undifferentiated and differentiated SC respectively. The GABA-B receptor and f-actin merge images revealed a different localization of GABA-B in the cell compartments, likely depending by high cAMP levels induced with forskolin. In all immunofluorecence the GABA-B detection was done with Alexa-488 (in green) while phalloidin-TRITC stained f-actin (1:250, in red). Merge images (in yellow) showing that f-actin/GABA-B co-localizations changed in distribution between undifferentiated and differentiated SC. Four high magnification images were done with LaserPix software (Bio-Rad Lab), based on Pearson's correlation coefficient with Costes'approach [reviewed in Bolte and Cordelieres (2006)]. LaserPix allows to superimpose a white mask onto the merge images, so that co-localized pixels of f-actin and GABA-B receptor are easily revealed as white dots. The average signal intensity/total pixels for white dots were calculated (104.15 undifferentiated vs. 92.24 differentiated SC, signal intensity/total pixels for GABA-B1; 106.91 undifferentiated vs. 83.54 differentiated SC, signal intensity/total pixels for GABA-B2). In undifferentiated SC GABA-B1 was present on cell protruding tips (arrows in A high magnification), while GABA-B2 was spread through the cytoplasm (C high magnification). After differentiation, the elongated processes of SC presented a white co-localization for both GABA-B receptors and f-actin (arrows in B and D high magnification). In these regions the co-localization of f-actin and GABA-B1 receptors was 94.1 ± 6.7%, while that of f-actin and GABA-B2 receptors was 86.3 ± 9.7%. GABA-B receptor was also analyzed in myelinating and non-myelinating SC. (E) Immunofluorescence in vivo in rat fibers of the sciatic nerve to evaluate the co-localization of GABA-B receptor and MAG [1:200, a specific protein of the adaxonal membrane of myelinating SC, (Scherer and Arroyo, 2002)]. Some GABA-B1 positive SC were distributed along axons (in green) without signs of co-localization with MAG (in red). (F) Same result was evident at high magnification, whereas GABA-B1 labeled the SC cytoplasm (asterisk), bordering the nerve fiber (arrowheads) as well as labeling the axon (arrows), but no sign of co-localization with MAG were shown. (G) In rat coronal section of rat sciatic nerve was assessed the co-localization of GABA-B receptor and GFAP [1:250, a specific marker of non-myelinating SC (Jessen and Mirsky, 1984)]. Several SC were double-stained for GABA-B1 (green) and GFAP (red) showing some co-localization in SC (yellow). (H) Immunofluorescence in vivo in rat saggital section of DRG to assess GABA-B receptor (in green) in the afferent sensitive fibers. Soma of sensitive neurons were GABA-B positive (arrows, left panel). The small sensitive fibers were labeled in red with FluoroMyelinTM (1:300). Several sensitive fibers were also immunopositive for GABA-B1 (green, left panel) with a preferential localization in non-myelinating SC (asterisk). No GABA-B1 was present in rat sciatic sections used as negative control (right panel, lack of GABA-B1 antibody). Nuclei were stained with dapi. Scale bars 20 μm.

The peculiar localization of GABA-B receptors on the elongations, tips, and bipolar processes, when the SC are induced to differentiate with forskolin, supports the proposed role of GABA-B receptors for SC differentiation. Moreover, this localization suggests “how” and “where” the GABA-B receptors are present. Both features may be an important requirement for the rearrangements associated to SC differentiation and for the establishment of the complex machinery regulating the myelination program in vivo.

The forskolin-induced GABA-B redistribution in SC seems to be specific, in contrast with the localization of neuregulins alpha and beta, which are differently affected by forskolin (Raabe et al., 2004). Indeed, in untreated SC both neuregulins are co-distributed throughout the cytoplasm, cell processes, and mostly excluded from the nucleus. After forskolin treatment the neuregulins change becoming either perinuclear or nuclear for alpha and beta, respectively (Raabe et al., 2004). In addition, the nerve growth factor (NGF) receptor is largely present at the distal ends of the SC, suggesting a supplementary function for this receptor during SC migration, development or ensheathing of axons (Assouline and Pantazis, 1989). However, the forskolin-induced GABA-B redistribution may be dependent from SC differentiation, but may be also a consequence of the reorganization of the SC actin cytoskeleton.

GABA-B Receptor is Expressed in Non-Myelinating and/or Myelinating SC

SC are very plastic cells that may switch between the undifferentiated and differentiated states. As a consequence SC may assume the non-myelinating and/or myelinating condition respectively (Jessen and Mirsky, 2005, 2008). This is relevant for the axon-glial crosstalk, for the development of sensory or motor functions, and for nerve regeneration after an injury.

The findings gathered from GABA-B1 knockout mice suggest the importance of GABA-B in non-myelinating SC regulating the sensory functions (Magnaghi et al., 2008), nevertheless the literature in this regard is still poor.

In theory, the SC that myelinate in vivo are generally considered comparable to SC that differentiate in vitro. In this context, rat nerve fibers were evaluated for the co-localization of GABA-B1 receptor and MAG, a specific protein localized on the inner/adaxonal membrane in the myelinating SC and in the Schmidt-Lanterman incisures of non-compact myelin (Scherer and Arroyo, 2002). Some GABA-B1 positive SC were present in the nerve, distributed along axons (Figure 2E in green) but no significant co-localization with MAG (in red) was evident, neither in the adaxonal membrane nor in the Schmidt-Lanterman incisures (Figures 2E,F in yellow). The lack of co-localization of GABA-B and MAG in vivo, indicates that GABA-B receptors are not preferentially involved in the SC committed to myelinate. Indeed, several SC in the coronal section of rat sciatic nerve were double-stained for GABA-B1 (green) and for the glial fibrillary acidic protein (GFAP, in red) showing co-localizations in SC (yellow in Figure 2G). Given that GFAP is a marker of non-myelinating SC in the PNS (Jessen and Mirsky, 1984), this result strength the preferential localization of GABA-B1 in these SC deputed to surround the axons of small sensitive neurons.

The localization of GABA-B1 in non-myelinating SC was further supported by the analysis of sensory DRG. The non-myelinated fibers are generally known to be sensitive fibers entering the DRG (Markenson, 1996). As expected, analyzing the coronal sections just at the border between the sensitive dorsal root and the DRG structure, the soma of some sensory neurons of DRG showed a bright immunopositivity for GABA-B receptors (arrows in Figure 2H, left panel). Contemporarily, also several sensitive fibers entering the DRG, which are light myelinated fibers (A delta fibers) or non-myelinated fibers (C fibers), were GABA-B1-immunopositive (green Figure 2H, left panel). However, the GABA-B1-immunopositive fibers were not tightly connected to the myelin rings (red Figure 2H, left panel) corroborating the preferential GABA-B1 localization in the non-myelinating SC that surround the groups of axons (green Figure 2H, left panel).

Although other analysis are needed to support this hypothesis, our results indicate an interesting participation of GABA-B receptors in the differentiation/de-differentiation processes occurring in SC. Indeed, the GABA-B receptors at the SC tips and edges may be relevant when the SC are committed to become non-myelinating SC.

Conclusion

The observations presented in this paper, gathered from the literature and from our findings, stated that GABA-B receptors are present and functional active in SC of the PNS. The GABA-B receptor implication in the commitment of SC into the myelination program might represent a fascinating functional significance for their localization on the elongated parts of the differentiated SC. However, the GABA-B-mediated control of the differentiation/de-differentiation may be primarily relevant when SC are committed to become non-myelinating SC, thus forming the peripheral nociceptive fibers. In our opinion this is the main significance of GABA-B1 presence in SC. The efforts are now toward the perspective to demonstrate the role of GABA-B1 in non-myelinating SC. However, some questions are still open. Does a specific GABA-B1 receptor ablation affect SC or sensory neurons? Is the GABA-B receptor expression developmentally regulated? Does it co-localize with other different GABA receptors and/or proteins? The answers to these questions will shed light on the complexity of the functional presence of GABA-B receptors in the PNS.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This work has been supported by Fondazione Cariplo (Grant n° 2010/501 to Valerio Magnaghi) and Association Francaise Contre les Myopathies (AFM Grant n° 14163/2012 to Valerio Magnaghi). We are deeply grateful to Dr. Laura Feltri (University at Buffalo, NY, USA) and Dr. Alessandro Faroni (The University of Manchester, UK) for helpful comments and paper revision.

References

Aguayo, A. J., Charron, L., and Bray, G. M. (1976). Potential of Schwann cells from unmyelinated nerves to produce myelin: a quantitative ultrastructural and radiographic study. J. Neurocytol. 5, 565–573.

Pubmed Abstract | Pubmed Full Text

Assouline, J. G., and Pantazis, N. J. (1989). Localization of the nerve growth factor receptor on fetal human Schwann cells in culture. Exp. Cell Res. 182, 499–512.

Pubmed Abstract | Pubmed Full Text

Barber, S. C., Mellor, H., Gampel, A., and Scolding, N. J. (2004). S1P and LPA trigger Schwann cell actin changes and migration. Eur. J. Neurosci. 19, 3142–3150.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bettler, B., and Tiao, J. Y. (2006). Molecular diversity, trafficking and subcellular localization of GABAB receptors. Pharmacol. Ther. 110, 533–543.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bhisitkul, R. B., Villa, J. E., and Kocsis, J. D. (1987). Axonal GABA receptors are selectively present on normal and regenerated sensory fibers in rat peripheral nerve. Exp. Brain Res. 66, 659–663.

Pubmed Abstract | Pubmed Full Text

Bolte, S., and Cordelieres, F. P. (2006). A guided tour into subcellular colocalization analysis in light microscopy. J. Microsc. 224, 213–232.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bowery, N. G., Bettler, B., Froestl, W., Gallagher, J. P., Marshall, F., Raiteri, M., et al. (2002). International Union of Pharmacology. XXXIII. Mammalian gamma-aminobutyric acid(B) receptors: structure and function. Pharmacol. Rev. 54, 247–264.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Brown, D. A., Adams, P. R., Higgins, A. J., and Marsh, S. (1979). Distribution of gaba-receptors and gaba-carriers in the mammalian nervous system. J. Physiol. (Paris) 75, 667–671.

Pubmed Abstract | Pubmed Full Text

Brown, D. A., and Marsh, S. (1978). Axonal GABA-receptors in mammalian peripheral nerve trunks. Brain Res. 156, 187–191.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Charles, K. J., Deuchars, J., Davies, C. H., and Pangalos, M. N. (2003). GABA B receptor subunit expression in glia. Mol. Cell. Neurosci. 24, 214–223.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Faroni, A., Mantovani, C., Shawcross, S. G., Motta, M., Terenghi, G., and Magnaghi, V. (2011). Schwann-like adult stem cells derived from bone marrow and adipose tissue express gamma-aminobutyric acid type B receptors. J. Neurosci. Res. 89, 1351–1362.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Faulstich, H., Trischmann, H., and Mayer, D. (1983). Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Exp. Cell Res. 144, 73–82.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Fernandez-Valle, C., Gorman, D., Gomez, A. M., and Bunge, M. B. (1997). Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination. J. Neurosci. 17, 241–250.

Pubmed Abstract | Pubmed Full Text

Fields, R. D., and Stevens-Graham, B. (2002). New insights into neuron-glia communication. Science 298, 556–562.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gatto, C. L., Walker, B. J., and Lambert, S. (2003). Local ERM activation and dynamic growth cones at Schwann cell tips implicated in efficient formation of nodes of Ranvier. J. Cell Biol. 162, 489–498.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ige, A. O., Bolam, J. P., Billinton, A., White, J. H., Marshall, F. H., and Emson, P. C. (2000). Cellular and sub-cellular localisation of GABA(B1) and GABA(B2) receptor proteins in the rat cerebellum. Brain Res. Mol. Brain Res. 83, 72–80.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jessen, K. R., and Mirsky, R. (1984). Nonmyelin-forming Schwann cells coexpress surface proteins and intermediate filaments not found in myelin-forming cells: a study of Ran-2, A5E3 antigen and glial fibrillary acidic protein. J. Neurocytol. 13, 923–934.

Pubmed Abstract | Pubmed Full Text

Jessen, K. R., and Mirsky, R. (2005). The origin and development of glial cells in peripheral nerves. Nat. Rev. Neurosci. 6, 671–682.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jessen, K. R., and Mirsky, R. (2008). Negative regulation of myelination: relevance for development, injury, and demyelinating disease. Glia 56, 1552–1565.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jessen, K. R., Mirsky, R., Dennison, M. E., and Burnstock, G. (1979). GABA may be a neurotransmitter in the vertebrate peripheral nervous system. Nature 281, 71–74.

Pubmed Abstract | Pubmed Full Text

Jones, K. A., Borowsky, B., Tamm, J. A., Craig, D. A., Durkin, M. M., Dai, M., et al. (1998). GABA(B) receptors function as a heteromeric assembly of the subunits GABA(B)R1 and GABA(B)R2. Nature 396, 674–679.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kaupmann, K., Huggel, K., Heid, J., Flor, P. J., Bischoff, S., Mickel, S. J., et al. (1997). Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors. Nature 386, 239–246.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kaupmann, K., Malitschek, B., Schuler, V., Heid, J., Froestl, W., Beck, P., et al. (1998). GABA(B)-receptor subtypes assemble into functional heteromeric complexes. Nature 396, 683–687.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kuner, R., Kohr, G., Grunewald, S., Eisenhardt, G., Bach, A., and Kornau, H. C. (1999). Role of heteromer formation in GABAB receptor function. Science 283, 74–77.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lee, M. M., Badache, A., and Devries, G. H. (1999). Phosphorylation of CREB in axon-induced Schwann cell proliferation. J. Neurosci. Res. 55, 702–712.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Li, Y., Gonzalez, M. I., Meinkoth, J. L., Field, J., Kazanietz, M. G., and Tennekoon, G. I. (2003). Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells. J. Biol. Chem. 278, 9585–9591.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Magnaghi, V. (2007). GABA and neuroactive steroid interactions in glia: new roles for old players? Curr. Neuropharmacol. 5, 47–64.

Pubmed Abstract | Pubmed Full Text

Magnaghi, V., Ballabio, M., Camozzi, F., Colleoni, M., Consoli, A., Gassmann, M., et al. (2008). Altered peripheral myelination in mice lacking GABAB receptors. Mol. Cell. Neurosci. 37, 599–609.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Magnaghi, V., Ballabio, M., Cavarretta, I. T., Froestl, W., Lambert, J. J., Zucchi, I., et al. (2004). GABAB receptors in Schwann cells influence proliferation and myelin protein expression. Eur. J. Neurosci. 19, 2641–2649.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Magnaghi, V., Ballabio, M., Consoli, A., Lambert, J. J., Roglio, I., and Melcangi, R. C. (2006). GABA receptor-mediated effects in the peripheral nervous system: a cross-interaction with neuroactive steroids. J. Mol. Neurosci. 28, 89–102.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Magnaghi, V., Procacci, P., and Tata, A. M. (2009). Novel pharmacological approaches to schwann cells as neuroprotective agents for peripheral nerve regeneration. Int. Rev. Neurobiol. 87, 295–315.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Markenson, J. A. (1996). Mechanisms of chronic pain. Am. J. Med. 101, 6S–18S.

Pubmed Abstract | Pubmed Full Text

Mirsky, R., and Jessen, K. R. (1999). The neurobiology of Schwann cells. Brain Pathol. 9, 293–311.

Pubmed Abstract | Pubmed Full Text

Morgan, L., Jessen, K. R., and Mirsky, R. (1991). The effects of cAMP on differentiation of cultured Schwann cells: progression from an early phenotype (04+) to a myelin phenotype (P0+, GFAP-, N-CAM-, NGF-receptor-) depends on growth inhibition. J. Cell Biol. 112, 457–467.

Pubmed Abstract | Pubmed Full Text

Morris, M. E., Di Costanzo, G. A., Fox, S., and Werman, R. (1983). Depolarizing action of GABA (gamma-aminobutyric acid) on myelinated fibers of peripheral nerves. Brain Res. 278, 117–126.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ng, G. Y., Mcdonald, T., Bonnert, T., Rigby, M., Heavens, R., Whiting, P., et al. (1999). Cloning of a novel G-protein-coupled receptor GPR 51 resembling GABAB receptors expressed predominantly in nervous tissues and mapped proximal to the hereditary sensory neuropathy type 1 locus on chromosome 9. Genomics 56, 288–295.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Olsen, R. W., and Sieghart, W. (2008). International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid(A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update. Pharmacol. Rev. 60, 243–260.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Olsen, R. W., Snowhill, E. W., and Wamsley, J. K. (1984). Autoradiographic localization of low affinity GABA receptors with [3H]bicuculline methochloride. Eur. J. Pharmacol. 99, 247–248.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pfaff, T., Malitschek, B., Kaupmann, K., Prezeau, L., Pin, J. P., Bettler, B., et al. (1999). Alternative splicing generates a novel isoform of the rat metabotropic GABA(B)R1 receptor. Eur. J. Neurosci. 11, 2874–2882.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pollard, T. D., and Borisy, G. G. (2003). Cellular motility driven by assembly and disassembly of actin filaments. Cell 112, 453–465.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Raabe, T. D., Deadwyler, G., Varga, J. W., and Devries, G. H. (2004). Localization of neuregulin isoforms and erbB receptors in myelinating glial cells. Glia 45, 197–207.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Scherer, S. S., and Arroyo, E. J. (2002). Recent progress on the molecular organization of myelinated axons. J. Peripher. Nerv. Syst. 7, 1–12.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Scherer, S. S., Deschenes, S. M., Xu, Y. T., Grinspan, J. B., Fischbeck, K. H., and Paul, D. L. (1995). Connexin32 is a myelin-related protein in the PNS and CNS. J. Neurosci. 15, 8281–8294.

Pubmed Abstract | Pubmed Full Text

Schuler, V., Luscher, C., Blanchet, C., Klix, N., Sansig, G., Klebs, K., et al. (2001). Epilepsy, hyperalgesia, impaired memory, and loss of pre- and postsynaptic GABA(B) responses in mice lacking GABA(B(1)). Neuron 31, 47–58.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sobue, G., Shuman, S., and Pleasure, D. (1986). Schwann cell responses to cyclic AMP: proliferation, change in shape, and appearance of surface galactocerebroside. Brain Res. 362, 23–32.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Suter, U., Snipes, G. J., Schoener-Scott, R., Welcher, A. A., Pareek, S., Lupski, J. R., et al. (1994). Regulation of tissue-specific expression of alternative peripheral myelin protein-22 (PMP22) gene transcripts by two promoters. J. Biol. Chem. 269, 25795–25808.

Pubmed Abstract | Pubmed Full Text

Trapp, B. D., Andrews, S. B., Wong, A., O'Connell, M., and Griffin, J. W. (1989). Co-localization of the myelin-associated glycoprotein and the microfilament components, F-actin and spectrin, in Schwann cells of myelinated nerve fibres. J. Neurocytol. 18, 47–60.

Pubmed Abstract | Pubmed Full Text

Weinberg, H. J., and Spencer, P. S. (1976). Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin production. Brain Res. 113, 363–378.

Pubmed Abstract | Pubmed Full Text

White, J. H., Wise, A., Main, M. J., Green, A., Fraser, N. J., Disney, G. H., et al. (1998). Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396, 679–682.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: GABA, actin, cAMP, myelin, non-myelinating cells

Citation: Procacci P, Ballabio M, Castelnovo LF, Mantovani C and Magnaghi V (2013) GABA-B receptors in the PNS have a role in Schwann cells differentiation? Front. Cell. Neurosci. 6:68. doi: 10.3389/fncel.2012.00068

Received: 03 October 2012; Accepted: 19 December 2012;
Published online: 10 January 2013.

Edited by:

Dieter Wicher, Max Planck Institute for Chemical Ecology, Germany

Reviewed by:

Barbara Wroblewska, Georgetown University, USA
Marc Landry, Bordeaux Segalen University, France

Copyright © 2013 Procacci, Ballabio, Castelnovo, Mantovani and Magnaghi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

*Correspondence: Valerio Magnaghi, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via G. Balzaretti 9, Milan 20133, Italy. e-mail: valerio.magnaghi@unimi.it

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