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Front. Cell. Neurosci. | doi: 10.3389/fncel.2018.00469

Methods for three-dimensional all-optical manipulation of neural circuits

  • 1INSERM U968 Institut de la Vision, France
  • 2Centre national de la recherche scientifique (CNRS), France
  • 3Sorbonne Universités, France

Optical means for stimulating and monitoring neuronal activity have provided a lot of insight in neurophysiology lately towards our understanding on how brain works. Optogenetic actuators, calcium or voltage imaging probes and other molecular tools combined with advanced microscopies allowed ‘all-optical’ readout and manipulation of neural circuits. Completion of this remarkable work evolves towards the three-dimensional (3D) manipulation of neural ensembles at high spatiotemporal resolution. Recently, original optical methods have been proposed for both activating and monitoring neurons in the 3D space, mainly via optogenetic compounds. Here, we review these methods and anticipate possible combinations among them.

Keywords: light-shaping, three-dimensional photostimulation, three-dimensional functional imaging, all-optical neuronal studies, optogenetics, neural circuits

Received: 04 Sep 2018; Accepted: 19 Nov 2018.

Edited by:

Marco Canepari, UMR5588 Laboratoire Interdisciplinaire de Physique (LIPhy), France

Reviewed by:

Marco L. Dal Maschio, Dipartimento di Scienze Biomediche, Università degli Studi di Padova, Italy
Albrecht Stroh, University Medical Centre, Johannes Gutenberg University Mainz, Germany  

Copyright: © 2018 Ronzitti, Emiliani and Papagiakoumou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Eirini Papagiakoumou, INSERM U968 Institut de la Vision, Paris, 75012, Île-de-France, France, eirini.papagiakoumou@parisdescartes.fr