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Front. Neurol. | doi: 10.3389/fneur.2018.00182

Combined analysis of interleukin-10 gene polymorphisms and protein expression in children with cerebral palsy

  • 1Third Affiliated Hospital of Zhengzhou University, China
  • 2Fudan University, China
  • 3Children’s Hospital of Zhengzhou, China
  • 4Sahlgrenska Academy, University of Gothenburg, Sweden

Background: Interleukin-10 (IL-10) is an important anti-inflammatory and immunosuppressive cytokine, and it has indispensable functions in both the onset and development of inflammatory disorders. The association between persistent inflammation and the development of cerebral palsy (CP) has attracted much attention.

Objective: The purpose of this study was to investigate whether IL-10 gene polymorphisms and plasma protein expression are associated with CP and to analyze the role of IL-10 in CP.

Methods: A total of 282 CP patients and 197 healthy controls were genotyped for IL-10 polymorphisms (rs1554286, rs1518111, rs3024490, rs1800871, and rs1800896). Among them, 95 CP patients and 93 healthy controls were selected for plasma IL-10 measurement.

Results: The differences in the rs3024490 (p = 0.033) and rs1800871 (p = 0.033) allele frequencies of IL-10 were determined between CP patients and controls. The frequencies of allele and genotype between CP patients with spastic tetraplegia and normal controls of IL-10 polymorphisms showed significant differences for rs1554286, rs151811, rs3024490, rs1800871, and rs1800896 (pallele = 0.015, 0.009, 0.006, 0.003, and 0.006, p genotype = 0.039, 0.018, 0.027, 0.012, and 0.03, respectively). The plasma IL-10 protein level in CP patients was higher than normal controls (9.13 ± 0.77 vs. 6.73 ± 0.63 pg/ml, p = 0.017). IL-10 polymorphisms and protein association analysis showed that the TT genotype had higher plasma IL-10 protein levels compared to the GG+GT genotype at rs3024490 (11.14 ± 7.27 vs. 7.44 ± 6.95 pg/ml, p = 0.045, respectively) in CP cases.

Conclusion: These findings provide an important contribution toward explaining the pleiotropic role of IL-10 in the complex etiology of CP.

Keywords: Cerebral palsy (CP), cytokine, Inflammation, Interleukin-10, Single nucleotide polymorphisms (SNPs)

Received: 13 Nov 2017; Accepted: 08 Mar 2018.

Edited by:

Masahiro Tsuji, National Cerebral and Cardiovascular Center, Japan

Reviewed by:

Brahim Tabarki, University of Sousse, Tunisia
Hiroko Morisaki, Sakakibara Heart Institute, Japan  

Copyright: © 2018 Xia, Chen, Bi, Song, Zhang, Wang, Zhu, Shang, Xu, Wang, Xing and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Changlian Zhu, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China,