Original Research ARTICLE
Evaluation of the amyloid beta-GFP fusion protein as a model of amyloid beta peptides-mediated aggregation: a study of DNAJB6 chaperone
- 1Faculty of Pharmacy, Department of Biochemistry, Beni-Suef University, Beni-Suef, Egypt
- 2Kasr Al Ainy Faculty of Medicine, Department of Medical Biochemistry, Cairo University, Cairo, Egypt
Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by the accumulation and aggregation of extracellular amyloid β (Aβ) peptides and intracellular aggregation of hyper-phosphorylated tau protein. Recent evidence indicates that accumulation and aggregation of intracellular amyloid β peptides may also play a role in disease pathogenesis. This would suggest that intracellular Heat Shock Proteins (HSP) that maintain cellular protein homeostasis might be candidates for disease amelioration. We recently found that DNAJB6, a member of DNAJ family of heat shock proteins, effectively prevented the aggregation of short aggregation-prone peptides containing large poly glutamines (associated with CAG repeat diseases) both in vitro and in cells. Moreover, recent in vitro data showed that DNAJB6 can delay the aggregation of Aβ42 peptides. In this study, we investigated the ability of DNAJB6 to prevent the aggregation of extracellular and intracellular Aβ peptides using transfection of human embryonic kidney 293 (HEK293) cells with Aβ-green fluorescent protein (GFP) fusion construct and performing western blotting and immunofluorescence techniques. We found that DNAJB6 indeed suppresses Aβ-GFP aggregation, but not seeded aggregation initiated by extracellular Aβ peptides. Unexpectedly and unlike what we found for peptide-mediated aggregation, DNAJB6 required interaction with HSP70 to prevent the aggregation of the Aβ-GFP fusion protein and its J-domain was crucial for its anti-aggregation effect. In addition, other DNAJ proteins as well as HSPA1a overexpression also suppressed Aβ-GFP aggregation efficiently. Our findings suggest that Aβ aggregation differs from poly glutamine (Poly Q) peptide induced aggregation in terms of chaperone handling and sheds doubt on the usage of Aβ-GFP fusion construct for studying Aβ peptide aggregation in cells.
Alzheimer’s disease (AD) is the most prevalent cause of dementia in the elderly. It is estimated that more than 35 million people are affected with AD worldwide and this number is expected to grow exponentially in the next years (Reitz et al., 2011; Huang and Mucke, 2012). The key pathological changes associated with AD brains are the deposition of extracellular amyloid plaques composed of amyloid beta (Aβ) peptides and deposition of intracellular neurofibrillary tangles composed of hyperphosphorylated tau protein and reactive microgliosis (Takata and Kitamura, 2012).
Aβ is produced in the brain after the cleavage of the transmembrane amyloid precursor protein (APP) by two sequential proteases. Initially, APP is cleaved by the β-secretase that releases the sAPPβ ectodomain and a C-terminal fragment named C99, which is subsequently cleaved by the complex γ-secretase enzyme into the APP intracellular domain (AICD) and Aβ peptides with different amino acid lengths (37–43 amino acids) of which the Aβ42 species are considered as more toxic (Shankar et al., 2009) and aggregation prone peptides (O’Brien and Wong, 2011; Zheng and Koo, 2011; Haass et al., 2012; Bignante et al., 2013). Interestingly, C99 fragments generated after the cleavage of APP by β secretase were found to play an early and crucial role in AD pathogenesis in addition to their role as substrates for the production of both Aβ and AICD (Lauritzen et al., 2012).
Aβ peptides are able to self-aggregate into numerous assemblies ranging from Aβ dimers, soluble oligomers, proto-fibrils and amyloid plaques, which are believed to exist in equilibrium and to have different toxic propensities (Shankar et al., 2009; Benilova et al., 2012). In the classical amyloid cascade hypothesis, it has been suggested that the accumulation of the Aβ peptides into the extracellular plaques is the central cause of AD (Hardy and Higgins, 1992; Selkoe, 2006). However, recent data also suggest a crucial role for the intracellular Aβ in the pathogenesis of this disease (Armogida et al., 2001; Wirths et al., 2004; LaFerla et al., 2007; Gouras et al., 2010). APP together with its proteolytic enzymes β and γ secretases are not only localized on the plasma membrane, but also on other intracellular membranes such as the Golgi network, endosomes and on mitochondrial membranes via which Aβ peptides are produced intracellularly. These additional intracellular sites for Aβ production may be limited to neurons (Xu et al., 1995; Pasternak et al., 2003; Yu et al., 2005). Interestingly, accumulation of intracellular Aβ was detected before the appearance of amyloid plaques in transgenic AD mice where it was associated with the onset of cognitive impairment (Billings et al., 2005; Knobloch et al., 2007). Also in patients with AD and Down’s syndrome, intracellular Aβ was detected in post-mortem brains (Cataldo et al., 2004). Furthermore, this intracellularly generated Aβ found to be more potent in causing neuronal cell death than extracellular Aβ (Kienlen-Campard et al., 2002). Some findings even suggest that Aβ plaques are a late consequence of the intracellular Aβ peptide generation and export (Gyure et al., 2001; Gouras et al., 2005) meaning that intracellular Aβ somehow initiate extracellular plaques formation (Cataldo et al., 1994; D’Andrea and Nagele, 2010).
If intracellular Aβ and its aggregation indeed are important in AD initiation, it would be worthwhile to test if and how (components of) the intracellular protein quality control system might be able to handle Aβ peptides. Recent data have suggested that especially DNAJB6, an ubiquitously expressed member of DNAJ family of heat shock proteins (HSPs), might be an extremely efficient suppressor of aggregation initiated by small peptides. In fact, DNAJB6 was shown to efficiently prevent the aggregation of poly glutamines (Poly Q) peptides in vitro (Månsson et al., 2014b) and in cells (Gillis et al., 2013). As such, it was found to be a very efficient suppressor of poly Q protein aggregation and toxicity in cells, in a Xenopus model of poly Q aggregation (Hageman et al., 2010) and in a Drosophila model of Huntington’s disease (Fayazi et al., 2006). Importantly, we recently found that DNAJB6 is very effective in preventing the fibrillation of Aβ42 peptides in vitro, where it inhibits the primary and secondary nucleation pathways of Aβ through binding to different Aβ aggregated species (Månsson et al., 2014a). Like all members of the DNAJ family, DNAJB6 has an N-terminal J-domain which contains a conserved histidine, proline, and aspartic acid residues (HPD motif) that is essential for the interaction with HSP70 and stimulation of its ATPase activity.
These results prompted us to investigate the effect of DNAJB6 overexpression on the aggregation induced by intracellular (using an Aβ-green fluorescent protein, GFP fusion protein) as well as extracellular Aβ peptides. We found that DNAJB6 indeed prevented the intracellular aggregation of Aβ-GFP. However, based on DNAJB6 mutant analyses and on the comparison with other HSP members, our results shed doubt on whether aggregation of Aβ-GFP fusion actually is an appropriate model for studying intracellular Aβ peptide aggregation.
Materials and Methods
The ubiquitin-fusion protein used in this study; Ub-Aβ42-GFP was a kind gift from Dr. Eric Reits (Department of Cell Biology and Histology, Academic Medical center, Amsterdam, Netherlands). Aβ peptides-ATTO 550 fibrils were a kind gift from Dr. Ronald Melki (Laboratoire d’Enymologie et Biochimie Structurales, Center National de la Recherche Scientifique, France). The chaperones constructs: pcDNA5/FRT/TO V5 DNAJB6b, pcDNA5/FRT/TO V5 DNAJB6b(H31Q), pcDNA5/FRT/TO V5 DNAJA1, pcDNA5/FRT/TO V5 DNAJB1 and pcDNA5/FRT/TO V5 HSPA1a were described before in Hageman et al. (2010). HSPBs constructs; FRT-TO-HSPB1, FRT-TO-HSPB5 and FRT-TO-HSPB7 were described in Vos et al. (2009).
Purification of the Recombinant Aβ42 Peptides
The DNA encoding Aβ42 peptide was amplified using the primers 5′-CCCGGGAATTCCATATGGACGCGGAATTTCGCCATGATAGCGGC-3′ and 5′-TCCGCGGGATCCCTACTATGCAATCACGACGCCTCCGACC-3′ that introduced an N-terminal Met codon. The amplified DNA was cloned into the pET3a vector (Novagen, Darmstadt, Germany) between the NdeI and BamH1 restriction sites and expressed in E. coli strain BL21(DE3) codon+ (Stratagene). Recombinant Aβ1-42 was purified as described in Walsh et al. (2009). For fibrillation, Met-Aβ1-42 was diluted in phosphate-buffered saline (PBS) to a concentration of 100 μM and incubated at 37°C for 5 days. Met-Aβ1-42 fibrils in PBS were labeled by addition of two molar excess of NHS-ester ATTO-550 (ATTO-TEC, Siegen, Germany). Labelling was performed following the manufacturer’s recommendations giving rise to Aβ42-ATTO 550. Unreacted dye was removed by three cycles of sedimentation at 50,000 g and suspension of the fibrils in PBS.
Cell Culture and Transfections
Human embryonic kidney cells stably expressing the tetracycline repressor, Flp–In T-REx human embryonic kidney 293 cells (HEK 293; Invitrogen, Carlsbad, CA, USA, Catalog number: R780-07) were grown in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO). The medium was supplemented with 10% fetal calf serum (Greiner Bio-one, Long wood, FL, USA) plus 100 units/ml penicillin and 100 mg/ml streptomycin (Invitrogen). The cells were grown at 37°C under a humidified atmosphere containing 5% CO2. Blasticidin (5 μg/ml, GIBCO, Invitrogen) was regularly added in the culture medium of the cells and tetracycline (1 μg/ml, Sigma) was added to switch on the expression of pcDNA5/ FRT/TO chaperones when needed. HEK293 cells were plated at density 2 × 105 cells/9.6 cm2 on 0.001% poly-L-lysine (Sigma) coated wells for 24 h before transfections. Usually 0.5–1 μg of Ub-Aβ42-GFP with or without different chaperones at 1:3 ratio were transfected into HEK293 cells by polyethylenimine (PEI) transfection reagent (1 μg/μl, Polysciences) for 48 h before cell lysis.
Cell Fractionation and Western Blotting
Cells were washed twice with ice-cold PBS and lysed into 2× Tris lysis buffer (100 mM Tris-HCl pH 7.5, 300 mM NaCl, 10 mM EDTA pH 8.0, 1% Triton X100) supplemented with Protease inhibitor cocktail (Roche Diagnostics, Germany). Cell lysates were incubated in ice for 30 min and centrifuged at 14,000 rpm at 4°C for 20 min. The supernatants were collected and used as soluble fractions while the pellet fractions were washed once with PBS and then dissolved into sodium dodecyl sulfate (SDS) in PBS buffer. Samples were mixed with 2× Laemmli sample buffer with 5% β-mercaptoethanol (Sigma) and boiled for 5 min. Samples were separated either on 12.5% glycine SDS-polyacrylamide gel electrophoresis (PAGE) to detect Aβ42-GFP, HSPs and β-actin or separated onto 12% Tricine-SDS PAGE to detect Aβ peptides according to Schägger (2006). After gel electrophoresis, the separated proteins were transferred into nitrocellulose membranes. The membranes were blocked with 5% dry milk in PBS with 0.1% Tween 20 (PBST) for 1 h at room temperature and incubated overnight at 4°C with the following primary antibodies: 6E10 (1:1000 in TBST, Covance), anti V5 (1:5000 in PBST, Invitrogen), anti β-actin (1:1000 in PBST, Abcam), anti HSPB1 (1:1000 in PBST, Stress Marq Biosciences), anti HSPB5 (1:1000 in PBST, Stress Marq Biosciences) and anti HSPB7 (1:1000, Abnova). The next day, the membranes were washed with PBST and incubated with anti mouse HRP-conjugated secondary antibody (1:5000 in PBST, GE Healthcare) for 1 h at room temperature. Enhanced chemiluminescence (ECL) was used for protein detection using ECL western blotting substrate kit (Thermoscientifc). Bands were visualized by exposure of the membranes to Amersham Hyperfilm ECL (GE Heath Care, UK).
One day before transfection, HEK293 cells were plated onto 0.001% poly L-lysine coated cover slips. Cells were transiently transfected with 0.5 μg Ub-Aβ42-GFP and either 1.5 μg of DNAJB6b-V5 or FRTTO for 48 h. In case of testing extracellular Aβ42 peptides, 1 μM of Aβ42-ATTO 550 fibrils were exogenously added after 24 h transfection into the culture medium of the cells for another 24 h. Cells were washed three times with PBS, fixed with 3.7% formaldehyde in PBS for 15 min and permeabilized with 0.2% Triton X-100 in PBS for another 15 min. Cells were blocked with 100 mM glycine for 10 min then with 3% bovine serum albumin (BSA) in PBST for 30 min. Cells were incubated with primary antibody: rabbit anti V5 (1:200 in PBS-T, Life Technology) overnight at 4°C. Coverslips were washed with PBS-T and incubated with anti rabbit Alexa Fluor 594 (1:250 in PBST, Life Technology) for 1 h at room temperature. Coverslips were washed with PBS-T and incubated with 0.2 μg/ml 4, 6-diamidino-2-phenylindole (DAPI) for 10 min to stain the nuclei. Coverslips were washed with PBS and mounted with Citifluor medium (Citifluor Ltd., London, UK). Extracellular Aβ42 peptides were detected by tracking the fluorescent tag, ATTO 550. Images were obtained with Leica TCS SP8 confocal laser scanning microscope with HC PL APO CS2 63×/1.4 oil objective.
Data are expressed as mean ± SE of at least two independent experiments. Densitometry of western blot bands was calculated using Image Studio Lite software, LI-COR Biosciences, USA. Student’s t test was used for calculation of the statistical significance where *P < 0.05, **P < 0.01 and ***P < 0.001.
Generation of Intracellular Amyloid Beta (Aβ)
In order to study the effect of the molecular chaperone DNAJB6 on intracellular Aβ aggregation, we initially transfected HEK293 cells with GFP-Ub-Aβ42 construct. Upon expression, this construct is cleaved by the endogenous deubiquitinating enzymes into GFP-Ub and free Aβ42 peptides. Unfortunately, we were unable to detect intracellular free Aβ42 peptides. Apparently, the Aβ42 peptides are rapidly degraded. But, more relevant to this study, we transfected HEK293 cells with an Ub-Aβ42-GFP construct that generates Aβ42-GFP fusion peptide (Figure 1A). We found that a substantial fraction of the Aβ42-GFP fusion peptides ends up in the Triton X-100 insoluble (P) fraction of the transfected cells (Figure 1B). Consistently, we found Aβ42-GFP to accumulate in Aβ42-positive puncta both in the cytosol and inside the nuclei of the transfected cells (Figure 1C), together suggesting that Aβ42-GFP is indeed aggregated. Of note, no Aβ42-GFP was detected as high molecular weight material (in the stacking gel) and we were unable to detect Aβ42-GFP aggregates in filter trap assays, suggesting that although it is aggregated, Aβ42-GFP did not form SDS-insoluble (amyloid-like) structures.
Figure 1. DNAJB6 prevents the aggregation of Aβ42-GFP. (A) Scheme of generation of Aβ42-GFP intracellularly. Cells were transfected with Ub-Aβ42-GFP. Upon expression, Ub-Aβ42-GFP is cleaved by endogenous ubiqutin C-terminal hydrolases after the RGG amino acid sequence to give rise to Ub and Aβ42-GFP at 1:1 ratio. (B) HEK293 cells were transfected with 0.5 μg Ub-Aβ42-GFP with FRTTO-DNAJB6b-V5 at 1:1, 1:2 ratio or FRTTO as a control (1:0 ratio). After 48 h, cells were lysed and separated into Triton X100 soluble (S) and pellet (P) fractions (left panel) or total lysate (right panel). A β42-GFP and DNAJB6-V5 were detected by Western blotting with 6E10 and anti V5 antibodies respectively. β-actin was used as a loading control. The quantification of pellet/Soluble ratio relative to FRTTO were depicted in the chart above the blot. Values represent mean ± SE of three independent experiments. (C) HEK293 cells were transfected with Ub-Aβ42-GFP and either DNAJB6-V5 at 1:3 ratio or FRTTO as a control. Cells were fixed and immunostained with α-V5 (red) antibody to detect DNAJB6-V5. α-GFP (green) was used to detect Aβ-GFP and DAPI (blue) was used for DNA staining. The panel shows confocal images of mock cells (upper panel), cells transfected with Ub-Aβ-GFP with FRTTO (middle panel), cells transfected with Ub-Aβ-GFP and DNAJB6 (lower panel). Scale bar = 5 μm. (D) HEK293 cell line, stably expressing DNAJB6 was transfected with 0.2, 0.3 or 0.5 μg of Ub-Aβ42-GFP. Tetracycline was added to switch on the expression of DNAJB6. Cell lysates were fractionated into Soluble (S) and Pellet (P) and analyzed using western blotting as in panel (B).
DNAJB6 Prevents the Aggregation of Aβ42-GFP
To investigate whether DNAJB6 can inhibit aggregation of the intracellularly generated Aβ42-GFP, we transfected HEK293 cells with Ub-Aβ42-GFP and FRTTO-DNAJB6-V5 at different ratios (1:1 and 1:2). We found that, in cells expressing DNAJB6, the fraction of TritonX-100 insoluble Aβ42-GFP decreases whilst its amount in the TX-100 soluble fraction increases (Figure 1B). In parallel, cells expressing the V5-tagged-DNAJB6 contained less Aβ42-GFP puncta and showed a more diffuse Aβ42-GFP signal (Figure 1C).
We next confirmed these findings using a HEK293 cell line stably expressing DNAJB6-V5 under the control of a tetracycline-regulated promoter. Transfecting this cell line with increasing concentrations of Ub-Aβ42-GFP plasmid lead to a dose dependent increase in Aβ42-GFP insolubilization, which was prevented when DNAJB6 expression was turned on (Figure 1D).
DNAJB6 Depends on HPD Motif to Prevent Aβ-GFP Aggregation
To explore if the J-domain of DNAJB6 is essential to prevent the aggregation of Aβ-GFP or not, we transfected the HEK293 cells with Ub-Aβ42-GFP and with either DNAJB6 (wild type, wt) or DNAJB6 with a point mutation (H31Q) in the conserved HPD motif to inactivate the J-domain. Strikingly, we found that the J-domain mutant (H31Q) was less effective than wt protein in preventing the aggregation of Aβ-GFP suggesting that DNAJB6 was clearly dependent on interaction with HSP70 (Figure 2A). It should also be noted that the efficiency of DNAJB6 wt and mutant protein to prevent Aβ-GFP aggregation was inversely related to steady state expression levels of the Aβ-GFP fusion protein (T-fractions in Figure 2A) since it was previously shown that J-domain is important for at least DNAJB1 and DNAJB2a (Bailey et al., 2002; Westhoff et al., 2005) and partly to DNAJB6 (Hageman et al., 2010) to prevent the aggregation of poly Q repeats through a mechanism dependent on HSP70 interaction followed by proteasomal degradation. This may explain the observed increase in the total Aβ-GFP protein when J-domain was mutated in DNAJB6 (H31Q). Additionally, Månsson et al. (2014a) found that DNAJB6 is interacting with the very early aggregates of Aβ peptides to prevent further aggregation and failing to do so leads to incorporation of DNAJB6 into the fibrils formation. This would explain the considerable amounts of DNAJB6 (H31Q) localized in the pellet fraction (Figure 2A).
Figure 2. DNAJB6 depends on HPD motif to prevent Aβ42-GFP aggregation. (A) HEK293 cells were transfected with Ub-Aβ42-GFP with either DNAJA1, DNAJB1, DNAJB6 (Wt), DNAJB6 (H31Q) mutants at 1:3 ratio for 48 h. Cells were lysed, fractionated into T, S, P and Western blotting was performed (B) HEK293 cells were transfected with Ub-Aβ42-GFP with either FRTTO (control) or HSPA1a at 1:3 ratio for 48 h. Quantification of the pellet/soluble ratio of Aβ-GFP relative to FRTTO was depicted in the charts above the blots. Values represent mean ± SE of two independent experiments. *P < 0.05, ***P < 0.001, ns = non significant.
Since the anti-aggregation activity of DNAJB6 showed a dependence on the HPD motif, which is conserved in all members of DNAJs, we also investigated whether or not other canonical members of DNAJ family could prevent the aggregation of Aβ-GFP. Overexpression of DNAJA1 and DNAJB1 result in protection against Aβ-GFP aggregation (Figure 2A). In accordance with this finding, overexpression of HSPA1a (a canonical member of HSP70) lead to a reduced aggregation of Aβ-GFP (Figure 2B). This indicates that the inhibition of Aβ-GFP aggregation by DNAJB6 is not unique but it is shared with other members of DNAJ family.
Canonical Members of HSPB Family Prevent the Aggregation of Aβ-GFP
Next, we tested a number of HSPB chaperones on Aβ-GFP aggregation. We found that HSPB7 did not reduce the insolubilization of Aβ-GFP and even enhanced it (Figure 3). In contrast, HSPB1 was found to be effective in reducing insolubilization of Aβ-GFP (Figure 3). Finally, HSPB5 which could enhance the refolding of heat denatured firefly luciferase (Vos et al., 2010), here it did not have an effect on Aβ-GFP aggregation. Together, these findings further support our notion that Aβ-GFP requires different chaperone handling than peptide fragments.
Figure 3. Canonical members of HSPB family prevent the aggregation of Aβ-GFP. Cells were transfected with HSPB1, HSPB5, HSPB7 or FRTTO at 1:3 ratio for 48 h. Quantification of the pellet/soluble ratio of Aβ-GFP relative to FRTTO was depicted in the chart above the blot. Values represent mean ± SE of two independent experiments. **P < 0.01, ns = non significant.
DNAJB6 Neither Prevent the Uptake of Nor Dissolve Exogenous Aβ42-ATTO Assemblies After they have been Taken Up
It has been suggested that the extracellular Aβ peptides re-enter the cells then trigger intracellular Aβ aggregation through a templating mechanism, thus contributing to neuronal toxicity (Nagele et al., 2002; Crews and Masliah, 2010). To test whether intracellular chaperone elevation either affects extracellular Aβ42 peptides or their stability within the cytosol after they have been taken up, cells were exposed to 1 μM of extracellularly added Aβ42 fibrils tagged with the fluorescent dye ATTO 550 to facilitate their tracking (Freundt et al., 2012). After 24 h, the cells were fixed and analyzed by confocal microscopy. As demonstrated by others (Hu et al., 2009; Friedrich et al., 2010), Aβ42 fibrils are internalized and form intracellular foci (Figure 4A). DNAJB6 overexpression neither prevented extracellularly added Aβ42 fibrils take-up and their subsequent intracellular foci formation nor the solubility of fibrils (Figure 4B). Indeed, western blot analyses showed that extracellularly added Aβ42 fibrils remained insoluble upon DNAJB6 overexpression (Figure 4E, compare lanes 7, 8 with lanes 11, 12).
Figure 4. Aβ42-GFP aggregates do not co-localize with extracellular Aβ42-ATTO peptides. (A) One micromolar of Aβ42-ATTO 550 fibrils were added into the culture medium of HEK293 cells for 24 h Cells were fixed and stained with DAPI for nuclear staining. Confocal images were acquired by Leica SP8 confocal microscope. Extracellular Aβ peptides can enter the cells and form intracelluar aggreagtes. (B) HEK293 cells were transfected with DNAJB6-V5 in 1:3 ratio. After 24 h transfection, 1 μM of Aβ42 fibrils were added into the culture medium of cells for another 24 h and cells were fixed and stained with anti V5. Confocal images show that DNAJB6 cannot prevent the extracellular Aβ mediated aggregation. (C) Cells were transfected with 1μg Ub-Aβ-GFP. After 24 h transfection, 1 μM of Aβ42 fibrils were added into the culture medium of cells for another 24 h Cells were fixed. Confocal images show that extracellular Aβ peptides do not co-localize with Aβ-GFP. (D) Cells were transfected with Ub-Aβ-GFP and DNAJB6 in 1:3 ratio for 24 h then 1 μM of Aβ42-ATTO 550 fibrils were added into the culture medium of HEK293 cells for another 24 h Cells were fixed and immunostained as mentioned above except that DNAJB6 is not stained for. Images show that DNAJB6 can still decrease the aggregation of intracellular Aβ42-GFP in presence of extracellular Aβ peptides. ATTO 550 was used to detect extracellular Aβ peptides, GFP for Aβ42-GFP, anti V5 for DNAJB6 and DAPI for nuclei. DIC: differential interference contrast. Scale bar = 5 μm. (E) Western blot of cells transfected and fractionated as mentioned above.
Aβ42-GFP Aggregates do not Co-Localize with Extracellular Aβ42-ATTO Peptides
Next, we tested whether the extracellularly added Aβ42 fibrils could seed the assembly of intracellular Aβ42-GFP, an effect that was clearly demonstrated for poly Q aggregates in a similar study (Ren et al., 2009). Surprisingly, we found that exogenous Aβ42 fibrils did not increase Aβ42-GFP aggregation. Confocal analyses did not show any co-localization between Aβ42-GFP and exogenous Aβ42-ATTO 550 fibrils (Figure 4C) suggesting that exogenous Aβ42 peptides do not template Aβ42-GFP aggregation. This is further confirmed by the observation that the amount of Aβ-GFP aggregates did not increase upon addition of exogenous Aβ fibrils (Figure 4E compare lanes 1, 2 with lanes 9, 10). From these observations, we suggest that the Aβ42-GFP fusion product may not reflect the same characteristics of Aβ42 peptide aggregation.
Interestingly, DNAJB6 was still able to prevent the aggregation of the intracellular Aβ42-GFP in the presence of exogenous Aβ peptides as revealed both by confocal imaging (Figure 4D) and by cell fractionation (Figure 4E compare lanes 3, 4 with lanes 5, 6).
In this study, we investigated the effect of the molecular chaperones, in particular DNAJB6, on the aggregation of Aβ associated with AD. We found that DNAJB6 effectively prevented the aggregation of Aβ-GFP that are generated intracellularly via a canonical dependence on J-domain. Interestingly, DNAJB6 could not neither affect exogenous Aβ fibrils take-up nor the stability of those peptides after take-up.
We had special interest in DNAJB6 because it was previously shown to strongly inhibit the aggregation of poly Q peptides (Månsson et al., 2014b) and Aβ42 peptides (Månsson et al., 2014a) in vitro. Since also intracellular pools of Aβ peptides were suggested to be involved in disease pathogenesis (Oddo et al., 2006; see also “Introduction” Section), we reasoned that DNAJB6 could be a potential repressor of intracellular Aβ42 aggregation and thus potentially of target for AD.
However, DNAJB6 and other HSPs exhibited different effects between poly Q peptides aggregation and Aβ-GFP aggregation. First, DNAJB6 depends on its J-domain and subsequent interaction with HSP70 to prevent the aggregation of Aβ-GFP while the serine rich region but not J-domain was crucial for DNAJB6 to prevent the aggregation of Poly Q stretches in HSP70 independent manner (Gillis et al., 2013). Second, HSPA1a that does not reduce poly Q aggregation in HEK293 cells or DNAJB1 and DNAJA1 that only marginally lead to protection against poly Q aggregation (Hageman et al., 2010), here they did reduce the aggregation of Aβ42-GFP. Third, HSPB7, that was very potent in reducing aggregation of poly Q proteins, and HSPB1 that did not reduce poly Q aggregation (Vos et al., 2010), both exhibited the opposite effect on Aβ-GFP aggregation.
Although Aβ42-GFP has been repeatedly used as a model to study Aβ peptides aggregation and to discover new inhibitors for Aβ aggregation (Caine et al., 2007; Park et al., 2009; Chakrabortee et al., 2012), our data suggest that Aβ42-GFP aggregation does not seem to reflect Aβ42 peptides aggregation. This is based on our observations that: (1) Aβ42-GFP does not form SDS-insoluble aggregates; (2) Aβ42-GFP aggregation is not seeded by exogenous Aβ42 peptides; (3) although DNAJB6 was effective in preventing the aggregation of Aβ42-GFP model, it was dependent on functional interaction with HSP70, unlike its mode of action as “peptide chaperone”; (4), other members of DNAJ family, HSPA1a and HSPB1 that were only marginally effective as peptide chaperones were equally effective as DNAJB6 in preventing Aβ42-GFP aggregation. This behavior of Aβ-GFP may be attributed to the existence of a GFP tag (M Wt = 27 kDa) that is several times bigger than the bound Aβ peptide (M Wt = 4 kDa). Hence, GFP tag affects the biochemical and physical characters of a small peptide/protein that mainly aggregates and depends on complex conformational aspects.
Consistent with the previous findings that demonstrated that cytosolic Aβ is mainly degraded by the proteasome (LaFerla et al., 2007; Hong et al., 2014), we found that Aβ-GFP was much accumulated in the cells when we inhibited the proteasome by MG132 (data not shown). Mainly because Aβ42 is a short lived substrate starting with a destabilized N-terminal (Asp) residue that targets it to the N-end rule pathway followed by proteasomal degradation (Brower et al., 2013). Therefore, alterations of the ubiquitin-proteasome system may adversely affect the extent of Aβ degradation. Nevertheless, a dysfunction in the endosomal-lysosomal proteolysis or any of the autophagy related genes was found to affect the neuronal functions and promotes the accumulation of Aβ and tau toxic proteins (Ihara et al., 2012).
Our notion that DNAJB6 cannot prevent the extracellular Aβ peptides from aggregation is compatible with our recent in vitro data which showed that DNAJB6 was not able to prevent the aggregation of Aβ peptides after a critical concentration of Aβ fibrils are already formed (6% or more of Aβ monomers are converted to fibrils) before introducing DNAJB6 chaperone into the reaction mixture (Månsson et al., 2014a). It is worthy to mention that we used Aβ oligomers in our initial experiments as extracellular source of Aβ peptides with intracellular over-expression of DNAJB6, however we did not find any prominent difference between the behavior of Aβ oligomers compared with Aβ fibrils. In addition, DNAJB6 was still unable to prevent the further aggregation of Aβ oligomers and no co-localization between Aβ oligomers and intracellular Aβ-GFP was detected. Perhaps, over expression of C99 fragments of APP seems to be a good alternative to generate intracellular Aβ accumulation and study the impact of HSPs.
Hence, we conclude that DNAJB6 is an effective chaperone in preventing the aggregation mediated by intracellularly generated Aβ-GFP fusion but not the aggregation mediated by extracellular Aβ peptides. Moreover, Aβ-GFP may not fully represent the characteristics of Aβ peptides aggregation. Whereas this does not preclude DNAJB6 as a potential modifier of and target in AD, other models for intracellular Aβ aggregation are needed to further test this.
Rasha Hussein: designed and performed the experiments, analyzed the data, and drafted the manuscript. Reem Hashem: drafted and revised the manuscript, Laila Rashed: drafted and revised the manuscript. All authors read and approved the final manuscript.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was sponsored by a grant provided by the Ministry of Higher Education and Scientific Research, Egypt to Rasha Hussein We deeply thank Prof. Harm Kampinga for giving Rasha Hussein the chance to perform the experiments in his laboratory at Department of Cell Biology, University Medical Center Groningen, Netherlands. We also thank Dr. Eric Reits (Academic Medical Center, Amsterdam, Netherlands) for providing Ub-Aβ42-GFP construct and Dr. Ronald Melki (Laboratoire d’Enymologie et Biochimie Structurales, Center National de la Recherche Scientifique, France) for providing Aβ-ATTO peptides.
Aβ, Amyloid β; AD, Alzheimer disease; APP, amyloid precursor protein; GFP, Green fluorescent protein; HSP, Heat shock protein; HEK293, Human embryonic kidney 293; Poly Q, Poly glutamines; Wt, Wild type.
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Keywords: Aβ-GFP, heat shock proteins, amyloid beta aggregation, Alzheimer’s disease, chaperones, DNAJB6
Citation: Hussein RM, Hashem RM and Rashed LA (2015) Evaluation of the amyloid beta-GFP fusion protein as a model of amyloid beta peptides-mediated aggregation: a study of DNAJB6 chaperone. Front. Mol. Neurosci. 8:40. doi: 10.3389/fnmol.2015.00040
Received: 23 April 2015; Accepted: 13 July 2015;
Published: 27 July 2015.
Edited by:Nicola Maggio, The Chaim Sheba Medical Center, Israel
Reviewed by:Nicolas Sergeant, Inserm UMR S1172, France
Eduard Korkotian, Weizmann Institute of Science, Israel
Copyright © 2015 Hussein, Hashem and Rashed. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rasha M. Hussein, Faculty of Pharmacy, Department of Biochemistry, Beni-Suef University, Salah Salem Street, 62511 Beni-Suef, Egypt, email@example.com