%A Pham,Trong Khoa %A Buczek,Weronika A. %A Mead,Richard J. %A Shaw,Pamela J. %A Collins,Mark O. %D 2021 %J Frontiers in Molecular Neuroscience %C %F %G English %K Oxidation,Oxidative Stress,Proteomics,neurodegenerative disease,ALS (Amyotrophic lateral sclerosis),Alzheimer's ​disease %Q %R 10.3389/fnmol.2021.678837 %W %L %M %P %7 %8 2021-June-09 %9 Review %# %! Neuroproteomics of Cysteine Oxidation %* %< %T Proteomic Approaches to Study Cysteine Oxidation: Applications in Neurodegenerative Diseases %U https://www.frontiersin.org/articles/10.3389/fnmol.2021.678837 %V 14 %0 JOURNAL ARTICLE %@ 1662-5099 %X Oxidative stress appears to be a key feature of many neurodegenerative diseases either as a cause or consequence of disease. A range of molecules are subject to oxidation, but in particular, proteins are an important target and measure of oxidative stress. Proteins are subject to a range of oxidative modifications at reactive cysteine residues, and depending on the level of oxidative stress, these modifications may be reversible or irreversible. A range of experimental approaches has been developed to characterize cysteine oxidation of proteins. In particular, mass spectrometry-based proteomic methods have emerged as a powerful means to identify and quantify cysteine oxidation sites on a proteome scale; however, their application to study neurodegenerative diseases is limited to date. Here we provide a guide to these approaches and highlight the under-exploited utility of these methods to measure oxidative stress in neurodegenerative diseases for biomarker discovery, target engagement and to understand disease mechanisms.