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Front. Physiol. | doi: 10.3389/fphys.2018.00107

Sex- and tissue-specific expression profiles of odorant binding protein and chemosensory protein genes in Bradysia odoriphaga (Diptera: Sciaridae)

 Yunhe Zhao1, Jinfeng Ding1, Zhengqun Zhang2, Feng Liu1, Chenggang Zhou3 and Wei Mu1*
  • 1College of Plant Protection, Shandong Agricultural University, China
  • 2College of Horticultural Science and Engineering, Shandong Agricultural University, China
  • 3College of Plant Protection, Shandong Agricultural University, China

Bradysia odoriphaga is an agricultural pest insect affecting the production of Chinese chive and other liliaceous vegetables in China, and it is significantly attracted by sex pheromones and the volatiles derived from host plants. Despite verification of this chemosensory behavior, however, it is still unknown how B. odoriphaga recognizes these volatile compounds on the molecular level. Many of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play crucial roles in olfactory perception. Here, we identified 49 OBP and 5 CSP genes from the antennae and body transcriptomes of female and male adults of B. odoriphaga, respectively. Sequence alignment and phylogenetic analysis among Dipteran OBPs and CSPs were analyzed. The sex- and tissue-specific expression profiles of 54 putative chemosensory genes among different tissues were investigated by quantitative real-time PCR (qRT-PCR). qRT-PCR analysis results suggested that 22 OBP and 3 CSP genes were enriched in the antennae, indicating they might be essential for detection of general odorants and pheromones. Among these antennae-enriched genes, 9 OBPs (BodoOBP2/4/6/8/12/13/20/28/33) were enriched in the male antennae and may play crucial roles in the detection of sex pheromones. Moreover, some OBP and CSP genes were enriched in non-antennae tissues, such as in the legs (BodoOBP3/9/19/21/34/35/38/39/45 and BodoCSP1), wings (BodoOBP17/30/32/37/44), abdomens and thoraxes (BodoOBP29/36), and heads (BodoOBP14/23/31 and BodoCSP2), suggesting that these genes might be involved in olfactory, gustatory or other physiological processes. Our findings provide a starting point to facilitate functional research of these chemosensory genes in B. odoriphaga at the molecular level.

Keywords: Bradysia odoriphaga, Odorant binding protein, Chemosensory protein, expression profiles analysis, transcriptomes

Received: 30 Nov 2017; Accepted: 02 Feb 2018.

Edited by:

Bin Tang, Hangzhou Normal University, China

Reviewed by:

Pablo Pregitzer, University of Hohenheim, Germany
Peng He, Guizhou University, China  

Copyright: © 2018 Zhao, Ding, Zhang, Liu, Zhou and Mu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Wei Mu, Shandong Agricultural University, College of Plant Protection, Tai'an, China, muwei@sdau.edu.cn