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Front. Physiol. | doi: 10.3389/fphys.2019.00908

Pinocembrin Protects Against Dextran Sulfate Sodium-induced Rats Colitis by Ameliorating Inflammation, Improving Barrier Function and Modulating Gut Microbiota

 Lin Hu1,  Chao Wu1, Mingchang Liu2,  Zijian Zhang3,  Maruthi Prasad E4, Yu Chen5* and  Kai Wang6*
  • 1Jiangsu Provincial Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, China
  • 2Chinese Academy of Inspection and Quarantine, China
  • 3Department of Forensic Medicine, School of Biology and Basic Medical Sciences, Soochow University, China
  • 4School of Biomedical Engineering, Health Science Center, Shenzhen University, China
  • 5Zhejiang Academy of Traditional Chinese Medicine, China
  • 6Institute of Apiculture Research (CAAS), China

Pinocembrin (PIN) is a natural flavonoid widely found in bee propolis with potent gastrointestinal protective effects. In consequence, PIN has great potential in preventing inflammatory bowel diseases while scant information is available. In this study, a dextran sulfate sodium (DSS)-induced rats ulcerative colitis model (3.5 % DSS in drinking water for 7 days) was applied to explore the protective effects of PIN on macroscopic colitis symptoms, inflammation, intestinal epithelial barrier function, and gut microbiota homeostasis. While DSS-treated rats showed severe colitis clinical symptoms and histological changes (colonic pathological damages and intestinal goblet cells loss), pre-administration of PIN (5 and 10 mg/kg, p.o.) for a week alleviated these symptoms. Pre-administration of PIN also suppressed the pro-inflammatory gene expressions and improved tight junction functions of colonic epithelial cells. Additionally, PIN administration reversed DSS-induced short chain fatty acid (SCFA) loss, and improved cecal digestion by increasing the gut microbial diversity assessed by 16S rRNA phylogenetic sequencing. Overall, our results suggest a wide spectrum of protective effects of PIN in preventing inflammatory bowel diseases.

Keywords: Pinocembrin, Colitis, Tight junction protein, Gut Microbiota, IBDS

Received: 08 Apr 2019; Accepted: 02 Jul 2019.

Edited by:

Jie YIN, Institute of Subtropical Agriculture (CAS), China

Reviewed by:

Qiyang Shou, Zhejiang Chinese Medical University, China
Qingbiao Xu, Huazhong Agricultural University, China
Cuong Tran, Health and Biosecurity (CSIRO ), Australia  

Copyright: © 2019 Hu, Wu, Liu, Zhang, E, Chen and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Yu Chen, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, 310000, Zhejiang Province, China, blow2000@163.com
Dr. Kai Wang, Institute of Apiculture Research (CAAS), Beijing, China, kaiwang628@gmail.com