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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.00921

Identification of five key genes involved in intrinsic apoptotic pathway from yellow catfish Pelteobagrus fulvidraco and their transcriptional responses to high fat diet (HFD)

 Dan-Dan Li1, Shi-Cheng Ling1,  Kun Wu1 and  Zhi Luo1*
  • 1Huazhong Agricultural University, China

The hypothesis of the present study is that apoptosis through intrinsic mitochondrial pathway may mediate high fat diet (HFD)-induced changes of metabolism of P. fulvidraco. To this end, we cloned the full-length cDNA sequences of Cycs, Apaf1, Casp9, Casp3a and Casp3b involved in mitochondria apoptotic pathway, and explored their mRNA tissue expressions and transcriptional responses to HFD. All of these members shared similar domains to their orthologous genes of vertebrates. They were constitutively expressed in all analyzed tissues, but varied from tissue to tissue. Compared to the control, HFD up-regulated the mRNA expression of partial genes among these five key genes (Cycs, Apaf1, Casp9, Casp3a and Casp3b) in mesenteric fat, intestine, ovary and kidney, indicating the induction of apoptosis in these tissues; in contrast, HFD down-regulated mRNA levels of partial genes among the five key genes (Cycs, Apaf1, Casp9, Casp3a and Casp3b) in heart, spleen and gill, indicating the inhibition of apoptosis in these tissues. The present study will facilitate further exploration into their functions of these genes at the molecular level and disclose the critical involvement of these genes against nutrient changes, indicating that the processes of apoptosis in various tissues may differentially be modified by HFD.

Keywords: Apoptosis, intrinsic mitochondrial pathway, Gene characterization., Pelteobagrus fulvidraco, high fat diet

Received: 13 Feb 2019; Accepted: 08 Jul 2019.

Edited by:

Menghong Hu, Shanghai Ocean University, China

Reviewed by:

Brad Buckley, Portland State University, United States
Jun Jiang, Sichuan Agricultural University, China  

Copyright: © 2019 Li, Ling, Wu and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Zhi Luo, Huazhong Agricultural University, Wuhan, China, luozhi99@mail.hzau.edu.cn