Contribution of TRPC Channels to Intracellular Ca2 + Dyshomeostasis in Smooth Muscle From mdx Mice

Duchenne muscular dystrophy (DMD) is an irreversible muscle disease characterized by a progressive loss of muscle function, decreased ambulation, and ultimately death as a result of cardiac or respiratory failure. DMD is caused by the lack of dystrophin, a protein that is important for membrane stability and signaling in excitable cells. Although vascular smooth muscle cells (VSMCs) dysfunction occurs in many pathological conditions, little is known about vascular smooth muscle function in DMD. We have previously shown that striated muscle cells, as well as neurons isolated from dystrophic (mdx) mice have higher intracellular Ca²⁺ ([Ca²⁺]_i) and Na⁺ ([Na⁺]_i) concentrations and decreased cell viability in comparison with wild type (Wt). Experiments were carried out in isolated VSMCs from mdx (a murine model of DMD) and congenic C57BL/10SnJ Wt mice. We found elevated [Ca²⁺]_i and [Na⁺]_i in VSMCs from mdx mice compared to Wt. Exposure to 1-oleoyl-2-acetyl-sn-glycerol (OAG), a TRPC3 and TRPC6 channel activator, induced a greater elevation of [Ca²⁺]_i and [Na⁺]_i in mdx than Wt VSMCs. The OAG induced increases in [Ca²⁺]_i could be abolished by either removal of extracellular Ca²⁺ or by SAR7334, a blocker of TRPC3 and TRPC 6 channels in both genotypes. Mdx and Wt VSMCs were susceptible to muscle cell stretch-induced elevations of [Ca²⁺]_i and [Na⁺]_i which was completely inhibited by GsMTx-4, a mechanosensitive ion channel inhibitor. Western blots showed a significant upregulation of TRPC1 -3, -6 proteins in mdx VSMCs compare to age-matched Wt. The lack of dystrophin in mdx VSMCs produced a profound alteration of [Ca²⁺]_i and [Na⁺]_i homeostasis that appears to be mediated by TRPC channels. Moreover, we have been able to demonstrate pharmacologically that the enhanced stretch-induced elevation of intracellular [Ca²⁺] and concomitant cell damage in mdx VSMCs also appears to be mediated through TRPC1, -3 and -6 channel activation.

Introduction

Duchenne muscular dystrophy (DMD) is an X-linked inherited neuromuscular disorder caused by mutations in the dystrophin gene (Hoffman et al., 1987). Dystrophin is localized in the plasmalemma of excitable cells connecting the cytoskeleton of the cell to the extracellular matrix (Ibraghimov-Beskrovnaya et al., 1992; Waite et al., 2012). While initial clinical manifestations of DMD are related to skeletal muscle weakness (Chakkalakal et al., 2005), the development of dilated cardiomyopathy occurs occasioning heart failure and death (Nigro et al., 1990) and a non-progressive cognitive impairment has been observed in DMD patients (Bresolin et al., 1994; Anderson et al., 2002). Disturbance of intracellular [Ca²⁺] has been reported in skeletal muscle from DMD patients (Lopez et al., 1987) and mdx mice (Turner et al., 1988; Altamirano et al., 2013, 2014), in cardiac cells from mdx mice (Mijares et al., 2014) and in cortical and hippocampal neurons isolated from mdx mice (Lopez et al., 2016).

Although X-linked neuromuscular pathologies have been extensively studied in striated muscle, the implications of lack of dystrophin in smooth muscle in patients with DMD and mdx mice have not been adequately studied. Studies on mdx dystrophic gastric and intestinal smooth muscle cells have revealed impaired nitrergic relaxation and an increase in spontaneous tone, which have been attributed to the impairment of intracellular Ca²⁺ homeostasis (Mule et al., 1999; Baccari et al., 2000; Mule and Serio, 2001). In addition, in DMD patients, dysfunction in small airways (Begin et al., 1980), constipation (Nowak et al., 1982), gastric dilatation, intestinal pseudo-obstructions and acute gastric dilatation (Leon et al., 1986; Barohn et al., 1988; Jaffe et al., 1990) have been described. Furthermore, endothelial cell damage, platelet adhesion, and aggregation in small blood vessels have been observed in DMD patients (Miike et al., 1987). However, the functional implications of the lack of dystrophin in vascular smooth muscle cells (VSMCs) are mostly unknown.

In the current study, we show for the first time that dystrophin deficiency of VSMCs leads to dysfunctional regulation of [Ca²⁺]_i and [Na⁺]_i, which appears to be mediated by an influx of these ions through the transient receptor potential canonical (TRPC) channels. Furthermore, mechanical stretch elicited a further elevation of [Ca²⁺]_i in VSMCs from mdx compared to Wt, which was inhibited by the removal of extracellular calcium and by TRPC channel blockade.

Materials and Methods

Animal Model

Wt (C57BL/10SnJ) and mdx (CS7BL/10ScSn-mdx) male (6 months old) mice were obtained from breeding colonies at the Mount Sinai Medical Center, from founders initially obtained from the Jackson Laboratory (Bar Harbor, ME). All protocols used in the study were performed following the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and approved by the institution (IACUC Protocol #19090).

Primary Culture of VSMC

Mdx and their Wt non-dystrophic littermates were euthanized using CO₂ or cervical dislocation. VSMCs were isolated using a modification of a previously described method (Ray et al., 2001). In brief, the aorta was dissected from its origin at the left ventricle to the iliac bifurcation, and the vessel was cut and placed in Hank’s Balanced Salt Solution (HBSS) (Thermo Fisher Scientific, FL, United States). Using a dissecting microscope, the fat tissue and the adventitia were removed; then, the aorta was irrigated with HBSS plus 2.5 μg/mL Fungizone (Thermo Fisher Scientific, FL, United States). The vessel was open longitudinally, and with sterile cotton swabbed, the endothelial layer of cells was gently removed and then cut into small segments (around 4 mm² each). The aorta segments were incubated in HBSS containing 1 mg/ml collagenase type 2 (Worthington Biochemical Corporation, NJ, United States) for 30 min. Then, the solution containing collagenase type 2 was replaced with a solution containing 1 mg/ml collagenase type 2 and 0.5 mg/ml elastase (Worthington Biochemical Corporation, NJ, United States). After the second digestion step, the remaining tissue was mechanically dissociated in the dish by flushing through a series of decreasing size fire-polished pipettes. Fresh HBSS was then added to stop the enzymatic digestion, and the cell suspension was centrifugated at 600 × g. The cell pellet was resuspended and centrifugated again at 600 × g and then transferred to a Matrigel-coated 24-well cell culture plate containing smooth muscle cell growth medium (SGM-2, Lonza, GA, United States). Isolated VSMC were cultured in a humidified atmosphere (37°C) and for 7–10 days after platting before experimentation.

Assessment of VSMC Functionality

The following criteria were used to judge the functionality of VSMCs: (i) no cell shortening was observed when they perfused with the Ca²⁺ containing Ringer solution (1.8 mM Ca²⁺) and (ii) they contracted in response to electrical stimuli (1 ms square pulse duration, ∼1.5 × threshold voltage).

Measurements of Resting [Ca²⁺]_i and [Na⁺]_i

Double-barreled Ca²⁺ and Na⁺ selective microelectrodes were prepared as described previously (Eltit et al., 2013). Single smooth muscle cells were impaled with either a Ca²⁺ – or Na⁺-selective double-barreled microelectrode, and their potentials were recorded via a high-impedance amplifier (WPI Duo 773 electrometer; World Precision Instruments, FL, United States). Criteria for successful impalement of single muscle cells included an (i) abrupt drop to a steady level of Vm more negative than −55 mV, (ii) a recording stable for both potentials (Vm and Ca potential) for at least 60 s and (iii) an quick return to baseline on the exit of the microelectrodes from the cell. The specific Ca^{2 +} potential (V_Cae) or Na⁺ potential (V_Nae) was obtained by subtracting the V_Ca potential or V_Na from the 3 M KCl microelectrode potential (Vm); Vm, and the specific Ca²⁺- Na⁺ potentials were stored in a computer for future analysis.

Muscle Mechanical Stretch

VSMCs were seeded on flexible-bottomed culture plates coated with poly-L-lysine (Flexcell International Corp., NC, United States). After 48 h to allow for cell attachment and spreading, Wt and mdx VSMCs were bathing with Ringer solution and then subjected to mechanical stretch elongation of 30 cycles/min (0.5 Hz), 20% elongation using a Flexcell FX 5000 tension system for 5 min. After the cyclic stretch, to estimate cell damage, the medium was collected for the determination of lactate dehydrogenase (LDH) activity (released by VSMCs) using the LDH kit from Sigma-Aldrich (St. Louis, MO, United States) according to the manufacturer’s instructions. Parallel series of Flex culture plates not subjected to stretching served as controls. At the time of collection of the medium for LDH determination, [Ca²⁺]_i or [Na⁺]_i was measured in the stretched VSMCs using ion-selective microelectrodes.

Western Blot Analysis

Mdx and Wt anesthetized mice were euthanized using CO₂. The aorta was dissected as described above (Primary culture of VSMC). Aortic total protein extraction was performed using a modified Millipore enzyme buffer with added 0.5% Triton-X-100 for 1 h digestion and lysis step. After proteins transfer from the gel, we spliced the membrane horizontally according to the molecular weight of proteins of interest. Then, individual membrane strips were incubated with the primary anti-TRPC1, TRPC3, TRPC6, and dystrophin antibodies (Uryash et al., 2015). The corresponding protein size was determined based on the protein standard marker. The levels of target protein(s) were normalized to loading control using the housekeeper protein β-actin.

Solutions

The normal Ringer’s solution contained the following (in mM): 135 NaCl, 5 KCl, 1.8 CaCl₂, 1 MgCl₂, 5 glucose, 3.6 NaHCO₃ (pH 7.4). In all experiments, Wt and mdx VSMCs were perfused and equilibrated with the Ringer’s solution aerated with a mixture of 95% O₂ and 5% CO₂ at 37°C. For the Ca²⁺ free solution CaCl₂ was omitted and 2 mM MgCl₂ and 1 mM EGTA were added in its place. 1-oleoyl-2-acetyl-sn-glycerol (OAG) (100 μM) a TRPC3, and TRPC6 channel activator, SAR7334 (0.1 and 1 μM) a TRPC3 and TRPC6 channel blocker, GsMTx4 (5 μM) a specific mechanosensitive channel inhibitor, nifedipine (10 μM) a selective voltage-gated Ca²⁺ channel blocker solutions were prepared from stock solutions.

Statistical Analysis

All values are reported as mean ± SD. Student’s t-test or analysis of variance (1-way ANOVA and Tukey’s post hoc tests were used to determine significance. p < 0.05 was considered statistically significant. n_mice: number of mice used experimentally, n_cell: represents the number of successful measurements carried out.

Results

[Ca²⁺]_i and [Na⁺]_i Dyshomeostasis in mdx Vascular Smooth Cells

Striated muscle cells from DMD patients and mdx mice (Lopez et al., 1987; Altamirano et al., 2013, 2014; Mijares et al., 2014) show elevated intracellular Ca²⁺ and Na⁺. Therefore, intracellular [Ca²⁺] and [Na⁺] were measured in VSMCs isolated from Wt and mdx mice using double-barreled ion-specific microelectrodes. [Ca²⁺]_i was elevated in mdx VSMCs (266 ± 27 nM, n = 45) compared to that observed in Wt cells (121 ± 3 nM, n = 41), (p < 0.001) (Figure 1). Similarly, [Na⁺]_i was higher in mdx VSMCs (14 ± 1.2 mM n = 25) than observed in Wt cells (8 ± 0.1 mM n = 25) (p < 0.001) (Figure 1).

FIGURE 1

Figure 1. Elevated [Ca²⁺]_i and [Na⁺]_i in mdx VSMCs. [Ca²⁺]_i or [Na⁺]_i was measured in quiescent VSMCs isolated from Wt, and mdx mice using double-barreled ion-specific microelectrodes. [Ca²⁺]_i and [Na⁺]_i were significantly higher in mdx than Wt VSMCs. For [Ca²⁺]_i measurements: n_mice = 3/experimental condition, n_cell = 41–48/genotype; For [Na⁺]_i measurements: n_mice = 3/experimental condition, n_cell = 25–27/genotype. Values are expressed as means ± S.D. Student’s t-test, ***p ≤ 0.001.

OAG a TRPC3 and TRPC6 Activator, Induced Elevation of [Ca²⁺]_i and [Na⁺]_i in VSMCs

To directly investigate the effect of diacylglycerol (DAG) on [Ca²⁺]_i and [Na⁺]_i in smooth muscle cells, VSMCs were exposed to the DAG analog 1-oleoyl-2-acetyl-sn-glycerol (OAG) which activates TRPC3 and TRPC6 channels (Hofmann et al., 1999). Incubation in OAG (100 μM) for 10 min produces an elevation of [Ca²⁺]_i and [Na⁺]_i in both genotypes. Figures 2A–D show representative measurements of the resting membrane potential (Vm) and [Ca²⁺]_i from Wt and mdx VSMCs before and after exposure to OAG. In Wt VSMCs OAG provoked an increase of [Ca²⁺]_i by 46%, from 121 ± 3 nM, n = 22, to 177 ± 19 nM, n = 25 (p < 0.001), and in mdx VSMCs by 73%, from 271 ± 29 nM, n = 25, to 470 ± 55 nM, n = 27 (p < 0.001) (Figure 3A). [Na⁺]_i was also significantly elevated in Wt by 25% and in mdx VSMC by 46% upon incubation in OAG (Figure 3B). We examined the possible involvement of the voltage-gated Ca²⁺ channels in the OAG-induced elevation of [Ca²⁺]_i by pretreating cultured VSMCs with nifedipine 10 μM, a specific voltage-gated Ca²⁺ channel blocker. Nifedipine had no significant effects on OAG-induced elevation of [Ca²⁺]_i in either Wt or mdx VSMCs (Supplementary Figure S1).

FIGURE 2

Figure 2. Simultaneous measurements of Vm and [Ca²⁺]_i in single smooth muscle cells from control Wt and mdx mice. Effects of OAG. Vm is the membrane potential recorded with a conventional microelectrode filled with 3 M KCI, and V_Cae is the potential recorded with the calcium-selective electrode after the subtraction of Vm. V_Cae potential represents cell intracellular [Ca²⁺]. In Wt, [Ca²⁺]_i was 124 nM, and the Vm was –62 mV before OAG treatment and represents the cell intracellular [Ca^{2 +} ] (A). After incubation in OAG 100 μM [Ca²⁺]_i increased to 187 nM with no effect on Vm (–63 mV) (B). The measurements of Vm and [Ca²⁺]_i before and after OAG treatment were carried in the same muscle cell. In the mdx muscle cell Vm: was –62 mV and [Ca²⁺]_i was 258 nM before OAG (C) and after OAG incubation, [Ca²⁺]_i rose to 492 nM after OAG, with no change in Vm (–65 mV) (D). The determinations of Vm and [Ca²⁺]_i before and after OAG treatment were carried in two distinct muscle cells.

FIGURE 3

Figure 3. OAG induces elevation of [Ca²⁺]_i and [Na⁺]_i. Exposure of quiescent Wt and mdx VSMc to 1-oleoyl-2-acetyl-sn-glycerol (OAG) 100 μM induced a significant elevation of [Ca²⁺]_i (A) and [Na⁺]_i (B), which were greater in mdx than Wt muscle cells. Over the horizontal axis are indicated the experimental conditions used to measure [Ca²⁺]_i and [Na⁺]_i. For [Ca²⁺]_i measurements: n_mice = 4/experimental condition, n_cell = 21–27/genotype. For [Na⁺]_i measurements: n_mice = 3/experimental condition, n_cell = 23–25/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test ***p ≤ 0.001.

Removal of Extracellular Ca²⁺ Reduced [Ca²⁺]_i and Prevented OAG-Induced Elevation of [Ca²⁺]_i

To establish the impact of extracellular Ca²⁺ ([Ca²⁺]_e) on OAG-induced elevation of [Ca²⁺]_i, Wt and mdx VSMCs were incubated for 5 min in Ca²⁺-free solution before OAG (100 μM) treatment. Exposure to reduced [Ca²⁺]_e significantly lowered [Ca²⁺]_i in Wt and mdx VSMCs but had a greater effect in mdx (−49%, from 271 ± 24, n = 15 to 138 ± 11, n = 18, p < 0.001) than in Wt (−18%, from 122 ± 3 nM, n = 15 to 100 ± 5 nM, n = 17, p < 0.001) VSMCs (Figure 4). In the absence of [Ca²⁺]_e, the previously observed rise in [Ca²⁺]_i elicited by OAG in Ca²⁺ replete buffer was completely inhibited in both Wt and mdx VSMCs (Figure 3). In addition to the decrease in [Ca²⁺]_i, incubation in Ca²⁺-free solution leads to a reversible depolarization of cell membrane potential in both genotypes of about 4–6 mV despite the presence of 2 mM Mg²⁺ (Supplementary Figure S2).

FIGURE 4

Figure 4. Removal of [Ca²⁺]_e prevents the OAG-induced elevation in [Ca²⁺]_i. Removal of extracellular Ca²⁺ significantly lowered the [Ca²⁺]_i and provoked the inhibition of OAG (100 μM) induced elevation of [Ca²⁺]_i in VSMc isolated from Wt and mdx mice. On the horizontal axis are indicated the experimental conditions used to measure [Ca²⁺]_i. For [Ca²⁺]_i measurements: n_mice = 4/experimental condition, n_cell = 15–18/genotype. Values are expressed as means ± S.D. for each condition. One-way ANOVA with Tukey’s post-test, ***p ≤ 0.001.

SAR7334 Reduced [Ca²⁺]_i and Abolished the Increases of [Ca²⁺]_i and [Na⁺]_i Elicited by OAG

To gain insight into molecular mechanisms resulting in [Ca²⁺]_i elevation upon exposure to OAG, we measured [Ca²⁺]_i in Wt and mdx VSMCs before and after incubation with SAR7334 which is a blocker of TRPC6 and TRPC3 channels (Maier et al., 2015), and then again after exposure to OAG (100 μM). Pretreatment with SAR7334 significantly lowered [Ca²⁺]_i in dose-dependent manner in both genotypes. Pretreatment with 0.1 μM SAR7334, a concentration that block mostly TRPC6 channels (Maier et al., 2015) reduced [Ca²⁺]_i in Wt by 6% and in mdx VSMCs by18% (Figure 5A). Preincubation with SAR7334 (1 μM) that blocks TRPC3 and 6 channels (Maier et al., 2015) provoked further reduction of [Ca²⁺]_i in Wt (15%) and mdx VSMCs (50%) (Figure 5B). The Figure 6 shows representative records of the effects of SAR7334 on [Ca²⁺]_i in Wt VSMCs (Figure 6B), and mdx VSMCs (Figure 6D). In addition, SAR7334 1 μM also reduced [Na⁺]_i in Wt (13%) and in mdx VSMCs (35%) (Figure 5C) and prevented any significant increase in [Ca²⁺]_i and [Na⁺]_i upon exposure to OAG in both genotypes (Figures 5B,C).

FIGURE 5

Figure 5. SAR7334 reduced [Ca²⁺]_i and [Na⁺]_i and blocked the elevation of [Ca²⁺]_i and [Na⁺]_i induced by OAG. [Ca²⁺]_i was measured in VSMc isolated from Wt and mdx mice before and after incubation in SAR7374 (0.1 μM or 1 μM), as well as after the exposure with SAR7374 and OAG (100 μM). (A) shows that preincubation in SAR7374 (0.1 μM) reduced significantly [Ca²⁺]_i and [Na⁺]_i in both genotypes. (B) illustrates that SAR7374 (1 μM) further reduced [Ca²⁺]_i and [Na⁺]_i and prevented the increase in intracellular [Ca²⁺] and [Na⁺] induced by OAG. (C) shows that SAR7374 (1 μM) reduced [Na⁺]_i in control and mdx VSMc and prevents the elevation of [Na⁺]_i induced by OAG. Over the horizontal axis are indicated the experimental conditions used to measure [Ca²⁺]_i and [Na⁺]_i. For [Ca²⁺]_i: n_mice = 3/experimental condition, n_cell = 17–20/genotype. For [Na⁺]_i: n_mice = 3/experimental condition, n_cell = 13–19/genotype. Values are expressed as means ± S.D. for each condition. One-way ANOVA with Tukey’s post-test, **p < 0.01, ***p ≤ 0.001.

FIGURE 6

Figure 6. Effects of SAR7334 on [Ca²⁺]_i in Wt and mdx VSMC. A typical response obtained with a conventional microelectrode (Vm) and a Ca²⁺-selective microelectrode (V_Cae) from a Wt and mdx VSMCs before and after SAR7334 (1 μM) treatment. (A) shows the recording from a Wt VSMC before SAR7334 treatment (Vm: –63 mV and [Ca²⁺]_i 124 nM) and (B) after SAR7334 incubation (Vm: –64 mV and [Ca²⁺]_i 98 nM). The Vm and [Ca²⁺]_i measurements show in (A,B) were carried in the same smooth muscle cell. (C,D) represent the determination of Vm and [Ca²⁺]_i before (Vm –63 mV and [Ca²⁺]_i 280 nM) and after SAR7334 application (Vm: –64 mV and [Ca²⁺]_i 147 nM) in a mdx VSMCs. The Vm and [Ca²⁺]_i measurements in show (C,D) were carried in the same smooth muscle cell.

Cyclic Stretch Provokes Larger Increase of [Ca²⁺]_i and [Na⁺]_i in mdx VSMCs

Stretching smooth muscle cells has previously been shown to increase [Ca²⁺]_i (Zou et al., 2002; Ducret et al., 2010). Numerous members of the TRPC channel family, especially TRPC1, TRPC3, and TRPC6 are considered to be mechanosensitive channels (Friedrich et al., 2012; Takahashi et al., 2013) and are therefore possible candidates for this increase. In our VSMC stretch experiments [Ca²⁺]_i and [Na⁺]_i increased in both genotypes; however, the magnitude of the increases in Ca²⁺ and Na⁺ were greater in mdx than Wt. In Wt VSMCs [Ca²⁺]_i was elevated by 39% from 121 ± 3 nM, n = 25 to 169 ± 18 nM, n = 23 (p < 0.001) (Figure 7A) and [Na⁺]_i by 31% from to 8 ± 0.1 mM, n = 10 to 11 ± 1 mM, n = 10 (p < 0.001) (Figures 7B). In mdx VSMCs [Ca²⁺]_i was elevated by 69% from 285 ± 25 nM, n = 25 to 482 ± 37 nM, n = 20 (p < 0.001) (Figure 7A) and [Na⁺]_i by 43% from to 14 ± 1.2 mM, n = 19 to 20 ± 1.8 mM, n = 12 (p < 0.001) (Figure 7B). To test whether the elevation of [Ca²⁺]_i associated with stretch was mediated by Ca²⁺ influx through sarcolemma Ca²⁺ channels, extracellular Ca²⁺ was removed and 2 mM MgCl₂ and 1 mM EGTA were added to the bathing supernatant (see section “Materials and Methods”). Under these conditions the increase in [Ca²⁺]_i in response to stretch was abolished in both Wt and mdx VSMCs (Figure 8A). Re-addition of extracellular Ca²⁺ before repeating the stretch protocol allowed recovery of the increase in both genotypes. These results suggest that a Ca²⁺ influx was involved in the elevation of [Ca²⁺]_i upon the mechanical stretch. To further dissect the mechanism involved in the stretch-induced elevation of [Ca²⁺]_i in VSMCs we tested the effect of GsMTx-4 (5 μM), which is known to inhibit mechanosensitive channels (Spassova et al., 2006; Bowman et al., 2007). GsMTx-4 completely inhibited the stretch-induced increases of [Ca²⁺]_i in both genotypes (Figure 8B). Additionally, we examined whether the stretch-induced elevation of [Ca²⁺]_i was mediated via L-type voltage-gated Ca²⁺ channels by incubating the VSMCs with the Ca²⁺ channel blocker nifedipine (10 μM). The stretch-induced increase in [Ca²⁺] was not modified by nifedipine in either genotype (Supplementary Figure S3).

FIGURE 7

Figure 7. Stretch induces elevation of [Ca²⁺]_i and [Na⁺]_i in VSMCs. Repetitive mechanical (30 cycles/min) elongation (20% of resting length) produced a significant elevation of [Ca²⁺]_i and [Na⁺]_i in Wt and mdx VSMc, however, the magnitude of the increase of intracellular Ca²⁺ and Na⁺ concentrations was greater in mdx than Wt (A,B). For [Ca²⁺]_i: n_mice = 4/experimental condition, n_cell = 20–28/genotype. For [Na⁺]_i: n_mice = 3/experimental condition, n_cell = 10–19/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

FIGURE 8

Figure 8. Reducing Ca²⁺ influx inhibits stretch-induced elevation of [Ca²⁺]_i in VSMCs. Removal of extracellular [Ca²⁺] (A) or blocking the mechanosensitive channels using GsMTx4 (5 μM) (B) reduced [Ca²⁺]_i and abolished the increase in [Ca²⁺]_i in response to stretch (20% of resting length) in both genotypes. For [Ca²⁺]_i: n_mice = 5/experimental condition, n_cell = 13–19/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

Cyclic Stretch Provokes Cell Damage in mdx VSMCs

Resting LDH activity in the supernatant from non-stretched mdx VSMCs (a marker of cell damage) was 35% greater than in the supernatant from Wt VSMCs (Figure 9). Muscle stretching increased LDH activity in both genotypes; however, the increase was more significant in mdx than Wt VSMCs (41% in Wt vs. 90% in mdx VSMCs) (Figure 9).

FIGURE 9

Figure 9. Stretch induces greater cell damage in mdx VSMCs. LDH activity in the supernatant from non-stretch VSMCs was higher in mdx than Wt. Muscle elongation increases LDH activity in both genotypes; however, it was much greater in mdx than Wt. For LDL: n_mice = 3/experimental condition, n_cell = 17–20/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

Measurements of Protein Expression

To determine whether the elevation of [Ca²⁺]_i and [Na⁺]_i and enhanced response to OAG observed in dystrophic VSMs was associated with changes in TRPC protein in the membrane, the expression of TRPC1, -3 and -6 were measured using Western blot analysis. Analysis of these blots demonstrated that TRPC1, TRPC3, and TRPC6 were significantly upregulated in VSMCs from mdx compared to Wt mice (Figures 10A,B and Supplementary Figure S4).

FIGURE 10

Figure 10. Expression of TRPC isoforms. Figure 10. Expression of TRPC isoforms. Representative fluorescent Western blot analysis of the expression of TRPC1, TRPC3, TRPC6, and dystrophin (DYSTR) proteins in Wt and mdx VSMCs (A). Densitometric analysis of individual experiment fluorescent Western blots shown in (B). Data were normalized to actin and expressed as mean optical unit values ± S.D. n_mice = 3. Paired t-test, **p ≤ 0.01, ***p ≤ 0.001.

Discussion

To the best of our knowledge, this is the first comprehensive study of [Ca²⁺]_i and [Na⁺]_i dysregulation in mdx VSMCs. The main findings in the present study are the following: (i) quiescent VSMCs isolated from mdx mice have [Ca²⁺]_i and [Na⁺]_i overload; (ii) the increase in [Ca²⁺]_i and [Na⁺]_i induced by exposure to OAG was greater in mdx than Wt VSMCs; (iii) Removal of extracellular Ca²⁺ or blockade of TRPC3 and -6 channels abolished the increases of [Ca²⁺]_i and [Na⁺]_i elicited by OAG; (iv) Muscle stretch-induced elevation of [Ca²⁺]_i, and [Na⁺]_i was significantly higher in mdx than Wt VSMCs and removal of extracellular Ca²⁺ or exposure to mechanosensitive channel blockers inhibited the increase in [Ca²⁺]_i linked to mechanical stretch in both mdx and Wt VSMCs; (v) Baseline and stretch-induced LDH leak was significantly higher in mdx than Wt VSMCs; (vi) Expression of TRPC1, -3, and -6 proteins was upregulated in mdx compared to Wt VSMCs.

Duchenne muscular dystrophy is a lethal muscle disease characterized by the absence of dystrophin, which leads to progressive membrane injury and subsequent changes in intracellular Ca²⁺ homeostasis and cellular dead (Ervasti et al., 1990). Dystrophin is the major component dystrophin-glycoprotein complex, which allows the interaction between the cytoskeleton and the and extracellular matrix (Ervasti and Campbell, 1993). Dystrophin is also present in the smooth muscle, playing a similar role than in skeletal muscle (North et al., 1993; Sharma et al., 2008). Deficiency of dystrophin in striated muscle cells results in alterations intracellular ion dyshomeostasis and muscle degeneration (Lopez et al., 1987; Danialou et al., 2001; Allen and Whitehead, 2011; Altamirano et al., 2012, 2014). In smooth muscle, the lack of dystrophin has been related with different alterations in the respiratory, gastrointestinal tract and the vascular bed (Miike et al., 1987; Barohn et al., 1988; Jaffe et al., 1990; Sun, 2015; Brown et al., 2018).

Intracellular calcium plays an essential role under physiological conditions to regulate many different processes in VSMCs (Berridge et al., 2003; Huang et al., 2018). Quiescent and healthy excitable cells maintain an [Ca²⁺]_i in the vicinity of 100–120 nM versus an extracellular [Ca²⁺] of 1.8 mM (Lopez et al., 1983; Mijares et al., 2014; Lopez et al., 2018). The activity of membrane ion channels, plasma membrane ATP-dependent Ca²⁺-pump, Na⁺/Ca²⁺ exchangers, and an endoplasmic reticulum Ca²⁺ ATPase preserve the concentration gradient (Karaki et al., 1997). Our data show that quiescent VSMCs isolated from mdx mice have an intracellular Ca²⁺ and Na⁺ overload compared with non-dystrophic Wt VSMCs. A substantial increase in [Ca²⁺]_i has been reported in intact skeletal muscle from DMD patients and an altered intracellular Ca²⁺ and Na⁺ homeostasis has been observed in the skeletal and cardiac muscle cells from mdx mice (Altamirano et al., 2014; Lopez et al., 2017, 2018). Chronic elevation in [Ca²⁺]_i may activate hydrolytic enzymes (proteases, nucleases, and lipases), and subsequently compromise energy production, intracellular ion regulation, ROS production and ultimately result in cell death (Nicotera and Orrenius, 1998; Ascah et al., 2011; Altamirano et al., 2013, 2014; Lopez et al., 2017). Prevention of chronic elevation of [Ca²⁺]_i may exert a myoprotective effect on mdx VSMCs precluding cell death.

The TRPC channels are expressed in vascular smooth muscle vessels playing diverse physiological cellular responses (Yip et al., 2004; Inoue et al., 2006). We have demonstrated that application of OAG, a membrane-permeable diacylglycerol analog which activates TRPC3 and TRPC6 channels (Hofmann et al., 1999; Liu et al., 2005) produced a robust elevation of [Ca²⁺]_i and [Na⁺]_i in Wt and mdx, however, the increment was more significant in mdx than Wt VSMCs. Western blots showed a significant upregulation of TRPC1 -3, -6 proteins in mdx VSMCs compare to age-matched Wt, which probably contribute to the observed intracellular Ca²⁺, and Na⁺ overload and also to the greater responsiveness to OAG found in mdx VSMCs.

Ca²⁺-free solution inhibited the observed rise in [Ca²⁺]_i induced by OAG and induced a reversible depolarization of cell membrane potential. The effect of removing extracellular Ca²⁺ on resting membrane potential in smooth muscle has been previously reported by other groups (Bulbring and Tomita, 1970; Kuriyama and Tomita, 1970). Furthermore, the incubation of VSCMs with SAR7334, a TRPC3 and TRPC6 blocker (Maier et al., 2015), reduced [Ca²⁺]_i in a dose-dependent manner and also blocked the increases in [Ca²⁺]_i and [Na⁺]_i elicited by OAG in both Wt and mdx VSMCs. Based on SAR7334 pharmacological dose blocking effect on TRPC3 and TRPC6 channels (Maier et al., 2015), we can speculate that the contribution of TRPC3 channels to the VSMCs intracellular Ca²⁺ dyshomeostasis is more significant than TRPC6 channels. The increase of [Ca²⁺]_i induced by OAG was not affected by the Ca²⁺ channel inhibitor nifedipine, which suggests that the activation of L-type Ca²⁺ channels is not part of the mechanism by which OAG induced elevation of [Ca²⁺]_i and [Na⁺]_i in VSMCs. Dysregulation of TRPC channels has been associated with diverse vascular pathologies (Mandegar et al., 2002; Yu et al., 2004; Kumar et al., 2006) which could explain, at least in part, the severe pulmonary and systemic hypertension reported in children and adolescents suffering from DMD (Yotsukura et al., 1988; Braat et al., 2015).

Previous studies have demonstrated that VSCM stretch induces elevation of [Ca²⁺]_i (Zou et al., 2002; Ducret et al., 2010). TRPC channels, especially TRPC1, TRPC3, and TRPC6, are considered as mechanosensitive channels (Friedrich et al., 2012; Takahashi et al., 2013). Furthermore, the Gq/11 protein has been recognized as mechanosensors involve in the myogenic vasoconstriction in VSMCs of small resistance arteries (Mederos et al., 2008, 2016). Gq/11-coupled receptors appear to be linked to the TRPC channels provoking the activation of TRPC channels in a G protein-dependent manner (Mederos et al., 2008). Here, we have shown evidence that a Ca²⁺ influx pathway activated by mechanosensors in VSMCs appears to be mediated by canonical cationic channel, which seems to be more critical in mdx VSMCs than Wt. Extracellular Ca²⁺ influx mediated by the voltage-dependent L-type Ca²⁺ channels has been suggested to intervene in VSMC stretch-mediated activation (Murase et al., 2001; Park et al., 2003; Ito et al., 2008). However, the fact that nifedipine did not inhibit stretch-induced [Ca²⁺]_i elevation does not support this hypothesis.

Basal LDH activity in the extracellular medium was higher in the supernatant from mdx compared to Wt VSMCs, which is consistent with the idea that the absence of dystrophin leads to chronic injury due to a lack of structural support at the sarcolemma. Besides, stretching further increased extracellular LDH activity, an indicator that this muscle stretch yielded some degree of cell injury in both genotypes. However, because the increase was higher in mdx than Wt, these data support the view that the lack of dystrophin makes VSCMs more sensitive to contraction-induced damage (Petrof et al., 1993; Grady et al., 1997; Brooks, 1998). The intracellular Ca²⁺ elevation after VSMCs stretch was suppressed entirely in both genotypes by removal of extracellular Ca²⁺ or GsMTx-4, indicating that this event is mediated by Ca²⁺ influx from the extracellular side which appears to be through a GsMTx-4 sensitive pathway.

Study Limitations

Despite the novelty of our study, some limitations should be acknowledged. First, we used a pharmacological approach to characterize the involvement of TRPC channels in the dysregulation of intracellular Ca²⁺ observed in VSMCs from mdx mice, and we did not study the functional aspects of TRPC channels. Secondly, we did not carry out experiments in which TRPC1, -3, and -6 channels were individually or collectively downregulated using siRNA. Therefore, we were unable to assess whether decreasing TRPC channel expression rescues or improves intracellular Ca²⁺ regulation in mdx VSMCs.

Conclusion

This study provides direct evidence of anomalous regulation of resting intracellular Ca²⁺ and Na⁺ in VSMCs from mdx mice. The imbalance of [Ca²⁺]_i and [Na⁺]_i appears to be mediated mostly through TRPC channels since their pharmacological blocking activity markedly protected mdx VSMCs (Figure 11). Further, we have demonstrated the presence of an abnormal stretch-induced elevation of [Ca²⁺]_i in mdx VSMCs, which also appears to be mediated by TRPC channels. The originality of our paper stands in revealing the relevance of TRPC channels in the pathology of VSMCs in mdx mice. TRPC channels could be promising targets to help manage symptoms and slow the progression of this devastating disease.

FIGURE 11

Figure 11. Transient receptor potential canonical channel in healthy and mdx VSMCs. Schematic representation of TRPC channels isoforms and the effect of diacylglycerol (DAG) on intracellular [Ca^{2 +} ] and [Na⁺] in healthy (A) and in mdx VSMC (B). Binding of the agonist to the G-protein-coupled receptor leads to phospholipase C (PLC) activation. The activation of PLC hydrolyzes phosphatidyl 4-5 biphosphate PI(4,5)P2 to produce diacylglycerol (DAG) and IP3. DAG contributes to TRPC3 and TRPC6 channels, but also TRPC1 activation under heteromeric complexes allowing Ca²⁺ and Na⁺ influx into the vascular muscle cell. A chronic increase in Ca²⁺ and Na⁺ flux through the upregulated TRPC channels in mdx VSMCs contribute to the observed elevate [Ca²⁺]_i and [Na⁺]_i.

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation, to any qualified researcher.

Ethics Statement

All protocols used in the study were performed following the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and approved by the IACUC of Mount Sinai Medical.

Author Contributions

JL and JA contributed to the conception and design of the study. JL, AU, GF, and EE performed the experiments. All authors contributed to the manuscript revision, read and approved the submitted version.

Funding

This work was supported by the AFM-Téléthon-France (Grant No. 21543).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We are grateful to Dr. M. Sackner and Dr. P. D. Allen for all the valuable comments.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphys.2020.00126/full#supplementary-material

FIGURE S1 | Nifedipine does not block the OAG-induced increase in [Ca²⁺] in VSMCs. Incubation with nifedipine (NIFE) 10 μM did not abolish the increase in [Ca²⁺]_i induced by OAG 100 μM in VSMCs. For [Ca²⁺]_i: n_mice = 3/experimental condition, n_cell = 10–17/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

FIGURE S2 | Effects of removal of extracellular Ca2 + on the resting membrane potential. The omission of the Ca²⁺ from the extracellular media induced a partial membrane depolarization (4–6 mV) in both genotypes. The reintroduction of Ca²⁺ to the bathing media reverses the observed depolarization. For Vm: n_mice = 3/experimental condition, n_cell = 20–26/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

FIGURE S3 | Stretching induced elevation of [Ca²⁺]_i in VSMCs is not inhibited by nifedipine. Exposure with nifedipine (NIFE) 10 μM did not prevent the stretch (20% of resting length) induces elevation of [Ca²⁺]_i in VSMCs. For [Ca²⁺]_i: n_mice = 3/experimental condition, n_cell = 9–14/genotype. Values are expressed as means ± S.D. for each condition. Student’s t-test, ***p ≤ 0.001.

FIGURE S4 | TRPC and Dystrophin protein levels in VSMCs. Each panel shows representative TRPC1, TRPC3, TRPC6 and Dystrophin protein expressions using corresponding fluorescent antibody. Data are presented as optical unit (OU) values normalized to Actin signal. Left Y axis shows MW sizes (kDa) of corresponding protein standard size markers. Right Y axis is labeled with name and size (kDa) of corresponding protein signal on the representative blot. Top X axis contains names of total protein extract samples loaded onto the representative gel.

References

Allen, D. G., and Whitehead, N. P. (2011). Duchenne muscular dystrophy–what causes the increased membrane permeability in skeletal muscle? Int. J. Biochem. Cell Biol. 43, 290–294. doi: 10.1016/j.biocel.2010.11.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Altamirano, F., Lopez, J. R., Henriquez, C., Molinski, T., Allen, P. D., and Jaimovich, E. (2012). Increased resting intracellular calcium modulates NF-kappaB-dependent inducible nitric-oxide synthase gene expression in dystrophic mdx skeletal myotubes. J. Biol. Chem. 287, 20876–20887. doi: 10.1074/jbc.M112.344929

PubMed Abstract | CrossRef Full Text | Google Scholar

Altamirano, F., Perez, C. F., Liu, M., Widrick, J., Barton, E. R., Allen, P. D., et al. (2014). Whole body periodic acceleration is an effective therapy to ameliorate muscular dystrophy in mdx mice. PLoS One 9:e106590. doi: 10.1371/journal.pone.0106590

PubMed Abstract | CrossRef Full Text | Google Scholar

Altamirano, F., Valladares, D., Henriquez-Olguin, C., Casas, M., Lopez, J. R., Allen, P. D., et al. (2013). Nifedipine treatment reduces resting calcium concentration, oxidative and apoptotic gene expression, and improves muscle function in dystrophic mdx mice. PLoS One 8:e81222. doi: 10.1371/journal.pone.0081222

PubMed Abstract | CrossRef Full Text | Google Scholar

Anderson, J. L., Head, S. I., Rae, C., and Morley, J. W. (2002). Brain function in Duchenne muscular dystrophy. Brain 125, 4–13. doi: 10.1093/brain/awf012

PubMed Abstract | CrossRef Full Text | Google Scholar

Ascah, A., Khairallah, M., Daussin, F., Bourcier-Lucas, C., Godin, R., Allen, B. G., et al. (2011). Stress-induced opening of the permeability transition pore in the dystrophin-deficient heart is attenuated by acute treatment with sildenafil. Am. J. Physiol. Heart Circ Physiol. 300, H144–H153. doi: 10.1152/ajpheart.00522.2010

PubMed Abstract | CrossRef Full Text | Google Scholar

Baccari, M. C., Romagnani, P., and Calamai, F. (2000). Impaired nitrergic relaxations in the gastric fundus of dystrophic (mdx) mice. Neurosci. Lett. 282, 105–108. doi: 10.1016/s0304-3940(00)00879-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Barohn, R. J., Levine, E. J., Olson, J. O., and Mendell, J. R. (1988). Gastric hypomotility in Duchenne’s muscular dystrophy. N. Engl. J. Med. 319, 15–18.

PubMed Abstract | Google Scholar

Begin, R., Bureau, M. A., Lupien, L., and Lemieux, B. (1980). Control of breathing in Duchenne’s muscular dystrophy. Am. J. Med. 69, 227–234.

Google Scholar

Berridge, M. J., Bootman, M. D., and Roderick, H. L. (2003). Calcium signalling: dynamics, homeostasis and remodelling. Nat. Rev. Mol. Cell. Biol. 4, 517–529. doi: 10.1038/nrm1155

PubMed Abstract | CrossRef Full Text | Google Scholar

Bowman, C. L., Gottlieb, P. A., Suchyna, T. M., Murphy, Y. K., and Sachs, F. (2007). Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology. Toxicon 49, 249–270. doi: 10.1016/j.toxicon.2006.09.030

PubMed Abstract | CrossRef Full Text | Google Scholar

Braat, E., Hoste, L., De Waele, L., Gheysens, O., Vermeersch, P., Goffin, K., et al. (2015). Renal function in children and adolescents with Duchenne muscular dystrophy. Neuromuscul. Disord. 25, 381–387. doi: 10.1016/j.nmd.2015.01.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Bresolin, N., Castelli, E., Comi, G. P., Felisari, G., Bardoni, A., Perani, D., et al. (1994). Cognitive impairment in Duchenne muscular dystrophy. Neuromuscul. Disord. 4, 359–369.

PubMed Abstract | Google Scholar

Brooks, S. V. (1998). Rapid recovery following contraction-induced injury to in situ skeletal muscles in mdx mice. J. Muscle Res. Cell Motil. 19, 179–187.

PubMed Abstract | Google Scholar

Brown, I. A. M., Diederich, L., Good, M. E., Delalio, L. J., Murphy, S. A., Cortese-Krott, M. M., et al. (2018). Vascular smooth muscle remodeling in conductive and resistance arteries in hypertension. Arterioscler. Thromb. Vasc. Biol. 38, 1969–1985. doi: 10.1161/ATVBAHA.118.311229

PubMed Abstract | CrossRef Full Text | Google Scholar

Bulbring, E., and Tomita, T. (1970). Effects of Ca removal on the smooth muscle of the guinea-pig taenia coli. J. Physiol. 210, 217–232. doi: 10.1113/jphysiol.1970.sp009205

PubMed Abstract | CrossRef Full Text | Google Scholar

Chakkalakal, J. V., Thompson, J., Parks, R. J., and Jasmin, B. J. (2005). Molecular, cellular, and pharmacological therapies for Duchenne/Becker muscular dystrophies. FASEB J. 19, 880–891. doi: 10.1096/fj.04-1956rev

PubMed Abstract | CrossRef Full Text | Google Scholar

Danialou, G., Comtois, A. S., Dudley, R., Karpati, G., Vincent, G., Des Rosiers, C., et al. (2001). Dystrophin-deficient cardiomyocytes are abnormally vulnerable to mechanical stress-induced contractile failure and injury. FASEB J. 15, 1655–1657. doi: 10.1096/fj.01-0030fje

PubMed Abstract | CrossRef Full Text | Google Scholar

Ducret, T., El Arrouchi, J., Courtois, A., Quignard, J. F., Marthan, R., and Savineau, J. P. (2010). Stretch-activated channels in pulmonary arterial smooth muscle cells from normoxic and chronically hypoxic rats. Cell Calcium 48, 251–259. doi: 10.1016/j.ceca.2010.09.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Eltit, J. M., Ding, X., Pessah, I. N., Allen, P. D., and Lopez, J. R. (2013). Nonspecific sarcolemmal cation channels are critical for the pathogenesis of malignant hyperthermia. FASEB J. 27, 991–1000. doi: 10.1096/fj.12-218354

PubMed Abstract | CrossRef Full Text | Google Scholar

Ervasti, J. M., and Campbell, K. P. (1993). A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin. J. Cell Biol. 122, 809–823. doi: 10.1083/jcb.122.4.809

PubMed Abstract | CrossRef Full Text | Google Scholar

Ervasti, J. M., Ohlendieck, K., Kahl, S. D., Gaver, M. G., and Campbell, K. P. (1990). Deficiency of a glycoprotein component of the dystrophin complex in dystrophic muscle. Nature 345, 315–319. doi: 10.1038/345315a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Friedrich, O., Wagner, S., Battle, A. R., Schurmann, S., and Martinac, B. (2012). Mechano-regulation of the beating heart at the cellular level–mechanosensitive channels in normal and diseased heart. Prog. Biophys. Mol. Biol. 110, 226–238. doi: 10.1016/j.pbiomolbio.2012.08.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Grady, R. M., Teng, H., Nichol, M. C., Cunningham, J. C., Wilkinson, R. S., and Sanes, J. R. (1997). Skeletal and cardiac myopathies in mice lacking utrophin and dystrophin: a model for Duchenne muscular dystrophy. Cell 90, 729–738. doi: 10.1016/s0092-8674(00)80533-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoffman, E. P., Brown, R. H. Jr., and Kunkel, L. M. (1987). Dystrophin: the protein product of the Duchenne muscular dystrophy locus. Cell 51, 919–928. doi: 10.1016/0092-8674(87)90579-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Hofmann, T., Obukhov, A. G., Schaefer, M., Harteneck, C., Gudermann, T., and Schultz, G. (1999). Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397, 259–263. doi: 10.1038/16711

PubMed Abstract | CrossRef Full Text | Google Scholar

Huang, H., Sun, Z., Hill, M. A., and Meininger, G. A. (2018). A calcium mediated mechanism coordinating vascular smooth muscle cell adhesion during KCl activation. Front. Physiol. 9:1810. doi: 10.3389/fphys.2018.01810

PubMed Abstract | CrossRef Full Text | Google Scholar

Ibraghimov-Beskrovnaya, O., Ervasti, J. M., Leveille, C. J., Slaughter, C. A., Sernett, S. W., and Campbell, K. P. (1992). Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix. Nature 355, 696–702. doi: 10.1038/355696a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Inoue, R., Jensen, L. J., Shi, J., Morita, H., Nishida, M., Honda, A., et al. (2006). Transient receptor potential channels in cardiovascular function and disease. Circ. Res. 99, 119–131. doi: 10.1161/01.res.0000233356.10630.8a

PubMed Abstract | CrossRef Full Text | Google Scholar

Ito, S., Kume, H., Naruse, K., Kondo, M., Takeda, N., Iwata, S., et al. (2008). A novel Ca2+ influx pathway activated by mechanical stretch in human airway smooth muscle cells. Am. J. Respir. Cell Mol. Biol. 38, 407–413. doi: 10.1165/rcmb.2007-0259oc

PubMed Abstract | CrossRef Full Text | Google Scholar

Jaffe, K. M., Mcdonald, C. M., Ingman, E., and Haas, J. (1990). Symptoms of upper gastrointestinal dysfunction in Duchenne muscular dystrophy: case-control study. Arch. Phys. Med. Rehabil. 71, 742–744.

PubMed Abstract | Google Scholar

Karaki, H., Ozaki, H., Hori, M., Mitsui-Saito, M., Amano, K., Harada, K., et al. (1997). Calcium movements, distribution, and functions in smooth muscle. Pharmacol. Rev. 49, 157–230.

Google Scholar

Kumar, B., Dreja, K., Shah, S. S., Cheong, A., Xu, S. Z., Sukumar, P., et al. (2006). Upregulated TRPC1 channel in vascular injury in vivo and its role in human neointimal hyperplasia. Circ. Res. 98, 557–563. doi: 10.1161/01.res.0000204724.29685.db

PubMed Abstract | CrossRef Full Text | Google Scholar

Kuriyama, H., and Tomita, T. (1970). The action potential in the smooth muscle of the guinea pig taenia coli and ureter studied by the double sucrose-gap method. J. Gen. Physiol. 55, 147–162. doi: 10.1085/jgp.55.2.147

PubMed Abstract | CrossRef Full Text | Google Scholar

Leon, S.H., Schuffler, M. D., Kettler, M., and Rohrmann, Ca. (1986). Chronic intestinal pseudoobstruction as a complication of Duchenne’s muscular dystrophy. Gastroenterology 90, 435–459.

PubMed Abstract | Google Scholar

Liu, X., Bandyopadhyay, B. C., Singh, B. B., Groschner, K., and Ambudkar, I. S. (2005). Molecular analysis of a store-operated and 2-acetyl-sn-glycerol-sensitive non-selective cation channel. Heteromeric assembly of TRPC1-TRPC3. J. Biol. Chem. 280, 21600–21606. doi: 10.1074/jbc.c400492200

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez, J. R., Alamo, L., Caputo, C., Dipolo, R., and Vergara, S. (1983). Determination of ionic calcium in frog skeletal muscle fibers. Biophys. J. 43, 1–4. doi: 10.1016/s0006-3495(83)84316-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez, J. R., Briceno, L. E., Sanchez, V., and Horvart, D. (1987). Myoplasmic (Ca²⁺) in Duchenne muscular dystrophy patients. Acta Cient. Venez. 38, 503–504.

Google Scholar

Lopez, J. R., Kolster, J., Uryash, A., Esteve, E., Altamirano, F., and Adams, J. A. (2016). Dysregulation of intracellular Ca²⁺ in dystrophic cortical and hippocampal neurons. Mol. Neurobiol. 55, 603–618. doi: 10.1007/s12035-016-0311-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez, J. R., Kolster, J., Uryash, A., Esteve, E., Altamirano, F., and Adams, J. A. (2018). Dysregulation of intracellular Ca²⁺ in dystrophic cortical and hippocampal neurons. Mol. Neurobiol. 55, 603–618. doi: 10.1007/s12035-016-0311-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez, J. R., Kolster, J., Zhang, R., and Adams, J. (2017). Increased constitutive nitric oxide production by whole body periodic acceleration ameliorates alterations in cardiomyocytes associated with utrophin/dystrophin deficiency. J. Mol. Cell. Cardiol. 108, 149–157. doi: 10.1016/j.yjmcc.2017.06.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Maier, T., Follmann, M., Hessler, G., Kleemann, H. W., Hachtel, S., Fuchs, B., et al. (2015). Discovery and pharmacological characterization of a novel potent inhibitor of diacylglycerol-sensitive TRPC cation channels. Br. J. Pharmacol. 172, 3650–3660. doi: 10.1111/bph.13151

PubMed Abstract | CrossRef Full Text | Google Scholar

Mandegar, M., Remillard, C. V., and Yuan, J. X. (2002). Ion channels in pulmonary arterial hypertension. Prog. Cardiovasc. Dis. 45, 81–114.

PubMed Abstract | Google Scholar

Mederos, Y., Schnitzler, M., Storch, U., Meibers, S., Nurwakagari, P., Breit, A., et al. (2008). Gq-coupled receptors as mechanosensors mediating myogenic vasoconstriction. EMBO J. 27, 3092–3103. doi: 10.1038/emboj.2008.233

PubMed Abstract | CrossRef Full Text | Google Scholar

Mederos, Y. S. M., Storch, U., and Gudermann, T. (2016). Mechanosensitive Gq/11 protein-coupled receptors mediate myogenic vasoconstriction. Microcirculation 23, 621–625. doi: 10.1111/micc.12293

PubMed Abstract | CrossRef Full Text | Google Scholar

Miike, T., Sugino, S., Ohtani, Y., Taku, K., and Yoshioka, K. (1987). Vascular endothelial cell injury and platelet embolism in Duchenne muscular dystrophy at the preclinical stage. J. Neurol. Sci. 82, 67–80. doi: 10.1016/0022-510x(87)90007-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Mijares, A., Altamirano, F., Kolster, J., Adams, J. A., and Lopez, J. R. (2014). Age-dependent changes in diastolic Ca(2+) and Na(+) concentrations in dystrophic cardiomyopathy: role of Ca(2+) entry and IP3. Biochem. Biophys. Res. Commun. 452, 1054–1059. doi: 10.1016/j.bbrc.2014.09.045

PubMed Abstract | CrossRef Full Text | Google Scholar

Mule, F., D’angelo, S., Tabacchi, G., Amato, A., and Serio, R. (1999). Mechanical activity of small and large intestine in normal and mdx mice: a comparative analysis. Neurogastroenterol. Motil. 11, 133–139. doi: 10.1046/j.1365-2982.1999.00142.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Mule, F., and Serio, R. (2001). Increased calcium influx is responsible for the sustained mechanical tone in colon from dystrophic (mdx) mice. Gastroenterology 120, 1430–1437. doi: 10.1053/gast.2001.24054

PubMed Abstract | CrossRef Full Text | Google Scholar

Murase, K., Naruse, K., Kimura, A., Okumura, K., Hayakawa, T., and Sokabe, M. (2001). Protamine augments stretch induced calcium increase in vascular endothelium. Br. J. Pharmacol. 134, 1403–1410. doi: 10.1038/sj.bjp.0704386

PubMed Abstract | CrossRef Full Text | Google Scholar

Nicotera, P., and Orrenius, S. (1998). The role of calcium in apoptosis. Cell Calcium 23, 173–180. doi: 10.1016/s0143-4160(98)90116-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Nigro, G., Comi, L. I., Politano, L., and Bain, R. J. (1990). The incidence and evolution of cardiomyopathy in Duchenne muscular dystrophy. Int. J. Cardiol. 26, 271–277. doi: 10.1016/0167-5273(90)90082-g

PubMed Abstract | CrossRef Full Text | Google Scholar

North, A. J., Galazkiewicz, B., Byers, T. J., Glenney, J. R. Jr., and Small, J. V. (1993). Complementary distributions of vinculin and dystrophin define two distinct sarcolemma domains in smooth muscle. J. Cell Biol. 120, 1159–1167. doi: 10.1083/jcb.120.5.1159

PubMed Abstract | CrossRef Full Text | Google Scholar

Nowak, T. V., Ionasescu, V., and Anuras, S. (1982). Gastrointestinal manifestations of the muscular dystrophies. Gastroenterology 82, 800–810. doi: 10.1016/0016-5085(82)90330-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Park, K. S., Kim, Y., Lee, Y. H., Earm, Y. E., and Ho, W. K. (2003). Mechanosensitive cation channels in arterial smooth muscle cells are activated by diacylglycerol and inhibited by phospholipase C inhibitor. Circ. Res. 93, 557–564. doi: 10.1161/01.res.0000093204.25499.83

PubMed Abstract | CrossRef Full Text | Google Scholar

Petrof, B. J., Shrager, J. B., Stedman, H. H., Kelly, A. M., and Sweeney, H. L. (1993). Dystrophin protects the sarcolemma from stresses developed during muscle contraction. Proc. Natl. Acad. Sci. U.S.A. 90, 3710–3714. doi: 10.1073/pnas.90.8.3710

PubMed Abstract | CrossRef Full Text | Google Scholar

Ray, J. L., Leach, R., Herbert, J. M., and Benson, M. (2001). Isolation of vascular smooth muscle cells from a single murine aorta. Methods Cell Sci. 23, 185–188.

PubMed Abstract | Google Scholar

Sharma, P., Tran, T., Stelmack, G. L., Mcneill, K., Gosens, R., Mutawe, M. M., et al. (2008). Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation. Am. J. Physiol. Lung Cell. Mol. Physiol. 294, L57–L68.

PubMed Abstract | Google Scholar

Spassova, M. A., Hewavitharana, T., Xu, W., Soboloff, J., and Gill, D. L. (2006). A common mechanism underlies stretch activation and receptor activation of TRPC6 channels. Proc. Natl. Acad. Sci. U.S.A. 103, 16586–16591. doi: 10.1073/pnas.0606894103

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, Z. (2015). Aging, arterial stiffness, and hypertension. Hypertension 65, 252–256. doi: 10.1161/hypertensionaha.114.03617

PubMed Abstract | CrossRef Full Text | Google Scholar

Takahashi, K., Kakimoto, Y., Toda, K., and Naruse, K. (2013). Mechanobiology in cardiac physiology and diseases. J. Cell. Mol. Med. 17, 225–232. doi: 10.1111/jcmm.12027

PubMed Abstract | CrossRef Full Text | Google Scholar

Turner, P. R., Westwood, T., Regen, C. M., and Steinhardt, R. A. (1988). Increased protein degradation results from elevated free calcium levels found in muscle from mdx mice. Nature 335, 735–738. doi: 10.1038/335735a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Uryash, A., Bassuk, J., Kurlansky, P., Altamirano, F., Lopez, J. R., and Adams, J. A. (2015). Antioxidant properties of whole body periodic acceleration (pGz). PLoS One 10:e0131392. doi: 10.1371/journal.pone.0131392

PubMed Abstract | CrossRef Full Text | Google Scholar

Waite, A., Brown, S. C., and Blake, D. J. (2012). The dystrophin-glycoprotein complex in brain development and disease. Trends Neurosci. 35, 487–496. doi: 10.1016/j.tins.2012.04.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Yip, H., Chen, W. Y., Leung, P. C., Kwan, H. Y., Liu, C., Huang, Y., et al. (2004). Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries. Histochem. Cell Biol. 122, 553–561. doi: 10.1007/s00418-004-0720-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Yotsukura, M., Miyagawa, M., Tsuya, T., Ishihara, T., and Ishikawa, K. (1988). Pulmonary hypertension in progressive muscular dystrophy of the Duchenne type. Jpn. Circ. J. 52, 321–326. doi: 10.1253/jcj.52.321

PubMed Abstract | CrossRef Full Text | Google Scholar

Yu, Y., Fantozzi, I., Remillard, C. V., Landsberg, J. W., Kunichika, N., Platoshyn, O., et al. (2004). Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension. Proc. Natl. Acad. Sci. U.S.A. 101, 13861–13866. doi: 10.1073/pnas.0405908101

PubMed Abstract | CrossRef Full Text | Google Scholar

Zou, H., Lifshitz, L. M., Tuft, R. A., Fogarty, K. E., and Singer, J. J. (2002). Visualization of Ca^{2 +} entry through single stretch-activated cation channels. Proc. Natl. Acad. Sci. U.S.A. 99, 6404–6409. doi: 10.1073/pnas.092654999

PubMed Abstract | CrossRef Full Text | Google Scholar