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Editorial ARTICLE

Front. Physiol., 19 November 2020 | https://doi.org/10.3389/fphys.2020.611117

Editorial: Endothelial Dynamics in Health and Disease

  • 1Centre for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
  • 2Department of Cardiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, Netherlands
  • 3Vascular Biology and Signalling Group, ProCURE, Oncobell Program, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain
  • 4CIBERONC, Instituto de Salud Carlos III, Madrid, Spain
  • 5Sanquin Research and Landsteiner Laboratory, Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, Netherlands
  • 6Department of Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Center, Location AMC, University of Amsterdam, Amsterdam, Netherlands

Editorial on the Research Topic
Endothelial Dynamics in Health and Disease

This special Research Topic of Frontiers in Physiology and Frontiers in Cell and Developmental Biology collates a series of review and research articles on the dynamic properties of endothelial cells, the key inner lining cells of blood and lymphatic vessels. Over the past decade, we have learnt that the vasculature is not a static tissue, but instead relies on the dynamic interactions of endothelial cells with each other, with the vascular microenvironment and with other cell types. For instance, new vessel formation occurs through collective migration and critically relies on rearrangements of interactions between endothelial cells. As such, endothelial cells are equipped with a variety of dynamic molecular systems that allow these cells to adapt to physiological and pathophysiological changes. Studying the systems that underlie endothelial dynamics brings fundamental insights into how vessels form and respond to their microenvironment. This knowledge is crucial to understand the development of cardiovascular disease, and it provides potential leads to guide the opening and closing of the vasculature for therapeutic applications in chronic inflammation and cancer.

Endothelial tissue integrity depends on adherens junctions that are based on the actin-anchored VE-cadherin receptor (Figure 1). The importance of the VE-cadherin complex has been widely studied in the context of endothelial barrier function (Orsenigo et al., 2012; Smith et al., 2020) (lymph)angiogenesis (Carmeliet et al., 1999; Bentley et al., 2014; Hägerling et al., 2018; Yang et al., 2019), inflammation (Wessel et al., 2014; van Steen et al., 2020), and flow sensing (Tzima et al., 2005; Lagendijk et al., 2017; Caolo et al., 2018). Duong and Vestweber now overview the complementary mechanisms that take place at endothelial cell-cell junctions beyond the VE-cadherin receptor in intact blood and lymphatic vessels. The importance of other adhesion receptors such as ESAM, Claudins and their anchoring to the dynamic actomyosin cytoskeleton is discussed as important contributors to endothelial integrity. In an original research paper by Werner et al., a new member of VE-cadherin-based endothelial junctions is identified: the actin-binding protein Coronin 1B. The authors investigated the molecular mechanism behind actomyosin relaxation and subsequent protrusive cytoskeletal activity evoked by Coronin 1B to seal the junctions.

FIGURE 1
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Figure 1. Endothelial cell dynamics is driven by the activity and organization of the actin cytoskeleton (Magenta), which anchors to VE-cadherin-based cell-cell junctions (Cyan).

Endothelial cell biology is controlled by VEGF signaling. Li et al. have investigated the importance of VEGF-A-mediated VEGFR2 activation and downstream VE-cadherin phosphorylation in experimental myocardial infarction models. Intriguingly, VEGFR2 Y949F knock-in mice are protected from cardiac edema, and these findings indicate that vascular leakage may be therapeutically targeted to improve heart tissue perfusion after infarction. The Neuropilins act as coreceptors for the VEGFRs and are well-known to support angiogenic signaling (Lampropoulou and Ruhrberg, 2014; Simons et al., 2016). Alghamdi et al. have investigated the angiogenic function of NRP2. The authors show that NRP2 promotes endothelial cell migration through regulating the trafficking of the fibronectin-binding integrin α5β1.

Integrins mediate the connection between the vascular extracellular matrix (ECM) and the contractile actomyosin cytoskeleton. Tight crosstalk occurs between endothelial adhesion structures during mechanotransduction: integrins translate forces from the ECM to actomyosin-based contractility, which in turn controls pulling forces on endothelial cell-cell contacts (Huveneers et al., 2015). Failure to respond to forces has direct consequences for vessel development and barrier function and underlies stiffness-related cardiovascular disease. Gordon et al. discuss the forces at play in vascular biology and provide a detailed overview of currently available advanced tools to study force-dependent events in blood and lymphatic endothelial cells cultured in vitro under physiological relevant conditions.

To be able to fully understand the impact of blood flow-derived forces on vascular development, one has to turn to in vivo experimental models. The review by Campinho et al. elaborates on the importance of various flow-derived mechanical cues on endothelial cell polarization, migration, cell shape changes, and proliferative functions at a single cell level. Endothelial responses to flow are outlined within the context of sprouting angiogenesis, intussusceptive angiogenesis, anastomosis, lumen formation, vessel stabilization, vessel size, and endothelial to haematopoietic transitions. An original research paper by Lv et al. addresses the correlations between flow-mediated dilation, polymorphisms in the gene encoding angiotensin-converting enzyme and endothelial secreted angiotensin II levels in pre-menopausal women.

It is increasingly clear that live imaging is necessary to understand the temporal endothelial activities in the developing vasculature and to this end zebrafish have become a powerful model system. Okuda and Hogan focus on new tools and insights in endothelial dynamics in vascular cell biology based on studies in zebrafish. A detailed toolbox of available genetic models to study endothelial properties during various stages of angiogenesis and their role in vascular development and disease are provided. Live imaging in zebrafish have also revealed exciting new concepts in our understanding of the origin of lymphatic vessels in tumors. In the review from Gutierrez-Miranda and Yaniv, the various endothelial and non-endothelial origins of lymphatics and their contribution to physiological development of the lymphatics and cancer lymphangiogenesis are discussed.

Genetic interference and fluorescence-based endothelial monitoring has led to impressive insights of endothelial cell dynamics during angiogenesis and vascular repair (Eilken and Adams, 2010; Simons et al., 2015; Angulo-Urarte et al., 2018). In the final review of this Research Topic Garcia-Gonzalez et al. compared the pro's and con's of the currently available state-of-the-art conditional genetic mouse models to investigate vascular dynamics at single cell and high molecular resolution. A historical perspective is provided as well as a comprehensive overview of the current sophisticated reporter tools to fluorescently label individual cells and perform lineage tracings in the vasculature (e.g., endothelial cells, pericytes, cardiomyocytes, or vascular smooth muscle cells) in combination with specific loss- or gain-of-function of molecular players.

Taken together, the field is poised to address many key outstanding questions in endothelial and vascular biology. Many previous studies have focused on the dynamics of endothelial cells during the initiation of sprouting in newly forming vasculature. But what happens at intermediate stages of vessel formation is less well-studied. Are comparable molecular systems responsible for vascular elongation and maintenance? Are there differences in endothelial dynamics between blood and lymphatic vessels? Which steps are decided on single cell level, and which require collective responses? We anticipate that current mechanistic insights and endothelial models allow the next generation of vascular researchers to address these caveats in the field of vascular biology and disease.

Author Contributions

This manuscript was written by all authors. All authors have performed editorial reviewing tasks for this Research Topic.

Funding

EJ was funded by Fonds Wetenschappelijk Onderzoek (G0B5920N) and EU H2020 (848109); MG by CERCA Programme/Generalitat de Catalunya for institutional support and by the research grants SAF2017-89116R-P (FEDER/EU) from MCIU (Spain) co-funded by European Regional Developmental Fund (ERDF), a Way to Build Europe; JB by the Netherlands Organization of Scientific Research VICI (016.196.632); and SH by the Netherlands Organization of Scientific Research ZonMW VIDI (016.156.327).

Conflict of Interest

MG is a consultant for Medicxi (London, UK) and has a research agreement with Merck & Co. Inc (North Wales, PA, USA) and with Venthera (Palo Alto, CA, USA).

The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

Angulo-Urarte, A., Casado, P., Castillo, S. D., Kobialka, P., Kotini, M. P., Figueiredo, A. M., et al. (2018). Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility. Nat. Commun. 9:4826. doi: 10.1038/s41467-018-07172-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Bentley, K., Franco, C. A., Philippides, A., Blanco, R., Dierkes, M., Gebala, V., et al. (2014). The role of differential VE-cadherin dynamics in cell rearrangement during angiogenesis. Nat. Cell Biol. 16, 309–321. doi: 10.1038/ncb2926

PubMed Abstract | CrossRef Full Text | Google Scholar

Caolo, V., Peacock, H. M., Kasaai, B., Swennen, G., Gordon, E., Claesson-Welsh, L., et al. (2018). Shear stress and VE-cadherin. Arteriosc. Thromb. Vasc. Biol. 38, 2174–2183. doi: 10.1161/ATVBAHA.118.310823

PubMed Abstract | CrossRef Full Text | Google Scholar

Carmeliet, P., Lampugnani, M. G., Moons, L., Breviario, F., Compernolle, V., Bono, F., et al. (1999). Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell 98, 147–157. doi: 10.1016/s0092-8674(00)81010-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Eilken, H. M., and Adams, R. H. (2010). Dynamics of endothelial cell behavior in sprouting angiogenesis. Curr. Opin. Cell Biol. 22, 617–625. doi: 10.1016/j.ceb.2010.08.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Hägerling, R., Hoppe, E., Dierkes, C., Stehling, M., Makinen, T., Butz, S., et al. (2018). Distinct roles of VE-cadherin for development and maintenance of specific lymph vessel beds. EMBO J. 37:e98271. doi: 10.15252/embj.201798271

PubMed Abstract | CrossRef Full Text | Google Scholar

Huveneers, S., Daemen, M. J., and Hordijk, P. L. (2015). Between Rho(k) and a hard place: the relation between vessel wall stiffness, endothelial contractility, and cardiovascular disease. Circ. Res. 116, 895–908. doi: 10.1161/CIRCRESAHA.116.305720

CrossRef Full Text | Google Scholar

Lagendijk, A. K., Gomez, G. A., Baek, S., Hesselson, D., Hughes, W. E., Paterson, S., et al. (2017). Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nat. Commun. 8:1402. doi: 10.1038/s41467-017-01325-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Lampropoulou, A., and Ruhrberg, C. (2014). Neuropilin regulation of angiogenesis. Biochem. Soc. Transac. 42, 1623–1628. doi: 10.1042/BST20140244

PubMed Abstract | CrossRef Full Text | Google Scholar

Orsenigo, F., Giampietro, C., Ferrari, A., Corada, M., Galaup, A., Sigismund, S., et al. (2012). Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo. Nat. Commun. 3:1208. doi: 10.1038/ncomms2199

PubMed Abstract | CrossRef Full Text | Google Scholar

Simons, M., Alitalo, K., Annex, B. H., Augustin, H. G., Beam, C., Berk, B. C., et al. (2015). State-of-the-art methods for evaluation of angiogenesis and tissue vascularization: a scientific statement from the American Heart Association. Circ. Res. 116, e99–e132. doi: 10.1161/RES.0000000000000054

PubMed Abstract | CrossRef Full Text | Google Scholar

Simons, M., Gordon, E., and Claesson-Welsh, L. (2016). Mechanisms and regulation of endothelial VEGF receptor signalling. Nat. Rev. Mol Cell Biol. 17, 611–625. doi: 10.1038/nrm.2016.87

PubMed Abstract | CrossRef Full Text | Google Scholar

Smith, R. O., Ninchoji, T., Gordon, E., André, H., Dejana, E., Vestweber, D., et al. (2020). Vascular permeability in retinopathy is regulated by VEGFR2 Y949 signaling to VE-cadherin. eLife 9:e54056. doi: 10.7554/eLife.54056

PubMed Abstract | CrossRef Full Text | Google Scholar

Tzima, E., Irani-Tehrani, M., Kiosses, W. B., Dejana, E., Schultz, D. A., Engelhardt, B., et al. (2005). A mechanosensory complex that mediates the endothelial cell response to fluid shear stress. Nature 437, 426–431. doi: 10.1038/nature03952

PubMed Abstract | CrossRef Full Text | Google Scholar

van Steen, A. C. I., van der Meer, W. J., Hoefer, I. E., and van Buul, J. D. (2020). Actin remodelling of the endothelium during transendothelial migration of leukocytes. Atherosclerosis (in press). doi: 10.1016/j.atherosclerosis.2020.06.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Wessel, F., Winderlich, M., Holm, M., Frye, M., Rivera-Galdos, R., Vockel, M., et al. (2014). Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nat. Immunol. 15, 223–230. doi: 10.1038/ni.2824

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, Y., Cha, B., Motawe, Z. Y., Srinivasan, R. S., and Scallan, J. P. (2019). VE-cadherin is required for lymphatic valve formation and maintenance. Cell Rep. 28, 2397.e4–2412.e4. doi: 10.1016/j.celrep.2019.07.072

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: endothelial cell, angiogenesis, VE-cadherin, cytoskeleton, mechanotransduction, VEGF signaling, lymphatic vessels, blood vessels

Citation: Jones EAV, Graupera M, van Buul JD and Huveneers S (2020) Editorial: Endothelial Dynamics in Health and Disease. Front. Physiol. 11:611117. doi: 10.3389/fphys.2020.611117

Received: 28 September 2020; Accepted: 20 October 2020;
Published: 19 November 2020.

Edited by:

Andreea Trache, Texas A&M University, United States

Reviewed by:

Irena Levitan, University of Illinois at Chicago, United States

Copyright © 2020 Jones, Graupera, van Buul and Huveneers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Stephan Huveneers, s.huveneers@amc.uva.nl