Abstract
Background: Hippocampal atrophy is a characteristic of Alzheimer’s disease (AD). However, alterations in structural connectivity (number of connecting fibers) between the hippocampus and whole brain regions due to hippocampal atrophy remain largely unknown in AD and its prodromal stage, amnestic mild cognitive impairment (aMCI).
Methods: We collected high-resolution structural MRI (sMRI) and diffusion tensor imaging (DTI) data from 36 AD patients, 30 aMCI patients, and 41 normal control (NC) subjects. First, the volume and structural connectivity of the bilateral hippocampi were compared among the three groups. Second, correlations between volume and structural connectivity in the ipsilateral hippocampus were further analyzed. Finally, classification ability by hippocampal volume, its structural connectivity, and their combination were evaluated.
Results: Although the volume and structural connectivity of the bilateral hippocampi were decreased in patients with AD and aMCI, only hippocampal volume correlated with neuropsychological test scores. However, positive correlations between hippocampal volume and ipsilateral structural connectivity were displayed in patients with AD and aMCI. Furthermore, classification accuracy (ACC) was higher in AD vs. aMCI and aMCI vs. NC by the combination of hippocampal volume and structural connectivity than by a single parameter. The highest values of the area under the receiver operating characteristic (ROC) curve (AUC) in every two groups were all obtained by combining hippocampal volume and structural connectivity.
Conclusions: Our results showed that the combination of hippocampal volume and structural connectivity (number of connecting fibers) is a new perspective for the discrimination of AD and aMCI.
Introduction
As an age-related neurodegenerative disease, Alzheimer’s disease (AD) is characterized by progressive cognitive impairment, especially memory decline. To date, no effective reversion treatment of duration has been found for AD. Thus, AD inevitably becomes a severe burden for families and society (Scheltens et al., ). Amnestic mild cognitive impairment (aMCI), the prodromal stage of AD, is now considered to maintain a conversion rate to AD of 10–15% every year (Petersen et al., ). Thus, it is widely accepted that early diagnosis and intervention in aMCI and AD are of great significance to improve the prognosis.
Notably, hippocampal atrophy is a key characteristic of AD. Generalized but predominantly medial and lateral shrinkage is present in the bilateral hippocampi in AD patients (Duan et al., ). However, although AD is considered a primary gray matter (GM) disorder, secondary changes in white matter (WM) are also significant and can be detected by diffusion tensor imaging (DTI). Fractional anisotropy (FA) and mean diffusivity (MD) are usually parameters used in DTI analysis, reflecting WM structural integrity. AD-related WM changes are characterized by decreased FA and increased MD, particularly affecting hippocampal, parahippocampal, and entorhinal connections, as well as the posterior cingulate cortex and anterior temporal lobe (Toepper, ). Furthermore, in patients with early AD, the volume of the hippocampal subiculum was positively associated with FA of the fornix, primarily consisting of axons from the subiculum (Wisse et al., ). Within patients with aMCI due to AD, significant correlations were also found between hippocampal volume and FA of the uncinate fasciculus, the bundle connecting the lateral orbitofrontal cortex with the hippocampus (RĂ©my et al., ). The above studies showed a relationship between hippocampal volume and connecting fiber integrity.
However, despite WM integrity changes, AD still involves alterations of structural networks, an equally important reason for cognitive impairment. Based on graph theory, structural networks showed disrupted integration and segregation in patients with AD (Dai et al., ), and involved topological disruptions in patients with MCI and AD predominantly in regions within the limbic system, prefrontal lobe, and occipital lobe (Lin et al., ). Compared with aMCI non-converters, aMCI converters to AD revealed more severe disruptions of structural connectivity, especially in the default-mode network (DMN) regions and connections (Sun et al., ). Despite studies at the global level, decreased nodal efficiency and weaker structural connections of the hippocampus were also observed in patients with AD (Wang et al., ). Moreover, a disease-epicenter disruption of structural connection was observed around entorhinal and hippocampal regions in patients with aMCI and AD (Mallio et al., ). However, secondary axon loss due to neuronal death inevitably results in the reduction of WM fibers number. Thus, hippocampal atrophy correspondingly leads to a reduction in the number of connecting fibers via neuronal loss. Because hippocampal connecting fibers are indispensable in cognitive function, the change in their number requires investigation. Unfortunately, an effective study on the relationship between hippocampal volume and the number of connecting fibers is still lacking.
Thus, we assume that the structural connectivity of the hippocampus not only shows a corresponding reduction accompanying hippocampal atrophy but also correlates with the decrease in hippocampal volume. To verify this hypothesis, we utilized high-resolution structural MRI (sMRI) and DTI data from 36 patients with AD, 30 patients with aMCI, and 41 normal control (NC) subjects in the current study. First, in the bilateral hippocampi, volume and structural connectivity with the whole-brain regions (sum of the number of connecting fibers) were analyzed. Second, correlations between hippocampal volume and ipsilateral structural connectivity were evaluated. Finally, the discrimination power provided by hippocampal volume, structural connectivity, and their combination were calculated.
Materials and Methods
Subject Enrollment and Neuropsychological Evaluations
Early in 1984, the criteria for the clinical diagnosis of AD were established by the National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer’s Disease and Related Disorders Association (NINCDS-ADRDA) work group on the basis of clinical manifestations (McKhann et al., ). Furthermore, due to the main pathology of AD being β-amyloid (Aβ) plaques, neurofibrillary tangles, and neuronal injury, the diagnostic guidelines for AD were created by the National Institute on Aging and Alzheimer’s Association (NIA-AA) using brain imaging and cerebrospinal fluid (CSF) biomarkers (Albert et al., ; McKhann et al., ) in 2011 and updated in 2018 (Jack et al., ). Low CSF Aβ42 (or the Aβ42/Aβ40 ratio; Blennow et al., ) and cortical amyloid positron emission tomography (PET) ligand binding (Villain et al., ) are valid indicators of Aβ plaques, whereas elevated CSF phosphorylated tau (p-tau; Buerger et al., ) and cortical tau PET ligand binding (Cho et al., ) are biomarkers of neurofibrillary tangles in AD.
Thus, due to the absence of CSF samples and brain amyloid or tau imaging by PET, patients were clinically diagnosed with probable AD when meeting the corresponding conditions of the NINCDS-ADRDA criteria (McKhann et al., ). However, patients were diagnosed with aMCI when conforming to the criteria described by Petersen (Petersen, ). Furthermore, the included AD and aMCI patients also fulfilled the core conditions of new diagnostic criteria for clinically probable AD and aMCI due to AD (Albert et al., ; McKhann et al., ). Clinical dementia rating (CDR) scores were used as a measure of dementia severity. AD patients with a CDR score of 1 or 2 and aMCI patients with a CDR score of 0.5 were included. The embodied NC subjects not only matched AD and aMCI patients in terms of sex and age but also had no complaints of memory decline or other cognitive impairment. All of the subjects in this study were 55–80 years old and had no contraindications for MRI examination. Moreover, subjects with other neurological or psychiatric diseases were strictly excluded.
Finally, 36 AD patients and 30 aMCI patients were recruited from the Department of Neurology Outpatient in the Second Medical Center of Chinese PLA General Hospital, as well as by poster. Forty-one age- and sex-matched NC subjects were recruited from the community by advertisement. These participants were all right-handed and Han Chinese. This study was approved by the Medical Ethics Committee of the Chinese PLA General Hospital. All participants signed consent forms by themselves or their legal guardians.
The clinical, physical, and neuropsychological assessments were performed within 1 week before the MRI scan. The neuropsychological assessment battery included the Mini-Mental State Examination (MMSE), the Montreal Cognitive Assessment (MoCA), and the Rey Auditory Verbal Learning Test (AVLT) and was completed by two senior neurologists. Total MMSE and MoCA scores were used to assess comprehensive cognitive impairment, whereas AVLT item scores specifically reflected memory decline. All of the neuropsychological tests showed worse performance with lower scores. In addition, the AD patients and aMCI patients were not taking medications such as cholinesterase inhibitors or memantine when undergoing MRI scans.
High-Resolution sMRI and DTI Data Acquisition
MRI examinations were all performed at the Department of Radiology in the Second Medical Center of Chinese PLA General Hospital, with a 3.0 T Siemens MR system (Skyra, Siemens, Germany) by one senior radiologist. A 20-channel head coil was selected. Subjects were given foam padding to minimize head motion when examined. Before high-resolution sMRI data were acquired, T2-weighted images were collected to exclude subjects with brain lesions.
High-resolution sMRI data implied sagittal T1-weighted structural images (192 continuous slices), which were acquired in each subject by magnetization-prepared rapid gradient echo (MPRGE) sequence with the following scan parameters: repetition time (TR) = 2,530 ms, echo time (TE) = 3.43 ms, inversion time (TI) = 1,100 ms, field of view (FOV) = 256 mm × 256 mm, acquisition matrix = 256 × 256, flip angle (FA) = 7°, and slice thickness = 1 mm. The three-dimensional images had a resolution of 1 mm × 1 mm × 1 mm.
After sMRI data were obtained, a single-shot echo planar imaging (EPI) sequence in the axial plane was used to acquire DTI data. The imaging parameters of EPI were as follows: TR = 8,000 ms; TE = 91 ms; acquisition matrix = 128 Ă— 128; FOV = 256 mm Ă— 256 mm; slice thickness = 3 mm with no gaps. A total of 50 contiguous slices were acquired for b values of 0 and 1,000 s/mm2 using gradients along 64 different diffusion directions.
Segmentation of Bilateral Hippocampi
Hippocampal segmentation was implemented by the FIRST (Patenaude et al., ) toolbox in FSL1. The bilateral hippocampi were segmented from individual high-resolution sMRI (T1-weighted structural images). T1-weighted structural images were also segmented into GM, WM, and CSF by the FAST (Zhang et al., ) toolbox in FSL. The normalized hippocampal volume for each participant was obtained by dividing the bilateral hippocampal volume by the sum of GM, WM, and CSF volume. (Figure 1A).
Figure 1
Preprocessing of DTI Data
Preprocessing of DTI data included eddy current and head motion correction, as well as estimation of the diffusion tensor and calculation of FA. All procedures were performed with the FDT toolbox in FSL1. Briefly, eddy current and head motion correction were carried out by applying an affine alignment of each diffusion-weighted image to the b0 image. Accordingly, the b-matrix was reoriented based on the transformation matrix (Leemans and Jones, ). The diffusion tensor elements were then estimated, and the corresponding FA value of each voxel was calculated (Basser and Pierpaoli, ).
Network Node Definition
An automated anatomic labeling (AAL) template (Tzourio-Mazoyer et al., ) was used to parcel the brain into 90 regions of interest (ROIs). The procedure has been previously described (Zalesky et al., ; Bai et al., ; Cao et al., ) and was performed using SPM8 software2. Briefly, individual T1-weighted images were coregistered to individual b0 images. The transformed T1-weighted images were then normalized to the ICBM152 T1 template in Montreal Neurological Institute (MNI) space. The inverse transformations were used to warp the AAL template from MNI space to the DTI native space.
WM Tractography
Diffusion tensor tractography was implemented with the Diffusion toolkit3 by using the “fiber assignment by continuous tracking (FACT)” method (Mori et al., ). The tracking procedure was seeded from the center of each voxel with a value of FA higher than 0.2 across the entire brain and was terminated if the turning angle was greater than 45 degrees or the fiber entered a voxel with a value of FA lower than 0.2. For each subject, tens of thousands of streamlines were generated to etch out all of the major WM tracts.
Definition of Network Edges
For network edge definition, two regions were considered structurally connected if there was at least one fiber streamline with two endpoints that were located in these two regions (Zalesky et al., ; Bai et al., ; Shu et al., ). Specifically, we defined the number of interconnecting streamlines ending in two regions as weights of the network edges. The hippocampal subnetwork was obtained by including nodes and edges connected with the hippocampus directly. The structural connectivity of the hippocampus was the sum of the number of connecting fibers (edges).
Statistical Analysis
Demographic factors, including age, gender, and years of education, were compared in the three groups using either ANOVA or the χ2 test. To determine the between-group differences in hippocampal volume and its structural connectivity with the whole brain (sum of the number of connecting fibers), a general linear model was performed with age, sex, and years of education as covariates. Then, we investigated the correlations between neuropsychological tests and hippocampal volume as well as its structural connectivity by partial correlation with the removal of the effect of age, gender, and years of education. In addition, the correlations between hippocampal volume and its structural connectivity were calculated with the same method. All of the above statistical analyses were performed with MATLAB software. We used 0.05 as a threshold of the P-value in all of the above analyses.
Discriminative Analysis
For discrimination analysis, we used a support vector machine (SVM) with a Gaussian function as a kernel function, which always outperforms kernel functions in classifying linear inseparable samples. The default settings of C = 1 and gamma are the reciprocal of the feature number in the LIBSVM Toolbox4 (Dosenbach et al., ; Iuculano et al., ). Leave-one-out cross-validation was used to evaluate the SVM model. Each subject was designated the test subject in turn, while the remaining subjects were used to train the SVM model. The model derived from the training subjects was then used to predict the test subject’s label. The accuracy, sensitivity, specificity, and area under the receiver operating characteristic (ROC) curve (AUC) were calculated to assess the model. To investigate the influence of different features, we compared the classification performance when concatenating the hippocampal volume and its structural connectivity (sum of connecting numbers) as features and a single type of feature.
Results
Demographic and Neuropsychological Assessment Data
Altogether, 107 participants, including 36 AD patients, 30 aMCI, patients and 41 NC subjects, were recruited in the current study. No significant differences were detected in mean age, gender ratio, or years of education among the three groups (P > 0.05). However, MMSE and MoCA scores of the three groups were significantly different (P < 0.001), with both scores of AD patients being the lowest, NC group being the highest, and aMCI patients being intermediate. In addition, the AVLT scores of immediate recall, delayed recall, recognition of primary words, and recognition of new words showed the same sequence (P < 0.001, Table 1).
Table 1
| AD (n = 36) | aMCI (n = 30) | NC (n = 41) | F | P | |
|---|---|---|---|---|---|
| Age (years) | 71.6 ± 8.8 | 69.4 ± 8.8 | 68.3 ± 6.8 | 3.05 | 0.218 |
| Gender (M/F) | 16/20 | 11/19 | 21/20 | 0.04 | 0.981 |
| Education (years) | 9.7 ± 4.5 | 12.1 ± 4.0 | 12.1 ± 4.3 | 0.45 | 0.800 |
| MMSE | 17.7 ± 6.0 | 26.8 ± 2.1 | 28.5 ± 1.4 | 86.69 | <0.001 |
| MoCAa | 14.4 ± 3.2 | 22.1 ± 2.6 | 26.6 ± 2.7 | 104.60 | <0.001 |
| AVLT-Immediate Recallb, c | 3.1 ± 1.4 | 4.3 ± 1.3 | 5.8 ± 1.2 | 34.26 | <0.001 |
| AVLT-Delayed Recallc, d | 0.6 ± 1.1 | 2.8 ± 2.1 | 5.8 ± 1.9 | 64.44 | <0.001 |
| AVLT-Recognition (primary words)e | 6.5 ± 3.4 | 8.3 ± 1.5 | 9.4 ± 1.1 | 14.55 | <0.001 |
| AVLT-Recognition (new words)f | 6.7 ± 3.3 | 9.1 ± 1.6 | 9.8 ± 0.7 | 18.35 | <0.001 |
Demographic and neuropsychological data in the AD, aMCI, and NC groups.
Age, gender, years of education, and neuropsychological tests were compared using either ANOVA or the χ2 test. aSeventeen AD patients, three aMCI patients and five NC subjects did not or refused to complete the test. bThe average of three immediate recall scores. cTen AD patients and three NC subjects did not or refused to complete the test. dThe score of delayed recall 5 min later. eTwelve AD patients and three NC subjects did not or refused to complete this test. fThirteen AD patients and three NC subjects did not or refused to complete this test. Abbreviations: MMSE, Mini-Mental State Examination; MoCA, Montreal Cognitive Assessment; AVLT, Auditory Verbal Learning Test; aMCI, amnestic mild cognitive impairment; NC, normal control.
Comparison of Hippocampal Volume
Among the AD, aMCI, and NC group comparisons, the volume of the bilateral hippocampi revealed the lowest value in AD patients, the intermediate value in aMCI patients, and the highest value in NC subjects (P < 0.001, Table 2). Furthermore, bilateral hippocampal volume showed most significant difference in AD vs. NC and AD vs. aMCI (P < 0.001, Figure 1B). However, in aMCI vs. NC, the difference in volume displayed P < 0.01 in the left hippocampus and P < 0.05 in the right hippocampus (Figure 1B).
Table 2
| AD | aMCI | NC | F | P | |
|---|---|---|---|---|---|
| L-hip volume | 2.87 ± 0.50 | 3.42 ± 0.53 | 3.75 ± 0.42 | 38.12 | <0.001 |
| R-hip volume | 3.08 ± 0.53 | 3.64 ± 0.62 | 4.02 ± 0.53 | 28.89 | <0.001 |
| L-hip structural connectivity | 55.94 ± 28.58 | 77.8 ± 32.84 | 83.27 ± 24.14 | 7.46 | <0.001 |
| R-hip structural connectivity | 87.94 ± 36.98 | 105.87 ± 41.0 | 114.83 ± 43.92 | 4.43 | 0.014 |
Mean volume and structural connectivity (sum of the number of connecting fibers) of the hippocampus in the AD, aMCI, and NC groups.
Abbreviations: L-hip, left hippocampal; R-hip, right hippocampal.
Comparison of Hippocampal Structural Connectivity
Compared with the NC group, significantly different structural connectivity was found between the left hippocampus and the following brain regions, precuneus (PCUN), thalamus (THA), lingual gyrus (LING), amygdala (AMYG), fusiform gyrus (FFG), parahippocampal gyrus (PHG), and temporal pole: middle temporal gyrus (TPOmid), and was found between the right hippocampus and inferior temporal gyrus (ITG) in addition to the above regions in AD and aMCI patients. Notably, a reduction in structural connectivity between the bilateral hippocampi and thalamus as well as the lingual gyrus showed relative preservation. (Figure 2A).
Figure 2
In addition, the sum of the number of connecting fibers in the left hippocampus was lowest in the AD group, moderate in the aMCI group, and highest in the NC group (P < 0.001, Table 2), with the same sequence in the right hippocampus (P < 0.05, Table 2). However, for comparison in every two groups, only the sum of the number of connecting fibers in the left hippocampus displayed most significant difference when comparing AD patients and NC subjects (P < 0.001, Figure 2B).
Correlations With Neuropsychological Tests
In the AD and aMCI groups, MMSE scores demonstrated a positive correlation with left hippocampal volume (R = 0.41, P < 0.001, Figure 3A) and right hippocampal volume (R = 0.36, P < 0.01, Figure 3B), whereas MoCA scores were positively correlated with left hippocampal volume (R = 0.54, P < 0.001, Figure 3C) and right hippocampal volume (R = 0.41, P < 0.01, Figure 3D). However, in AD and aMCI patients, no significant correlation between neuropsychological tests and structural connectivity (sum of the number of connecting fibers) was found in the right or left hippocampus.
Figure 3
Moreover, in every three groups, the correlations between neuropsychological tests and hippocampal volume, as well as those between neuropsychological tests and structural connectivity of the hippocampus, all showed no significance.
Correlations Between Hippocampal Volume and Its Structural Connectivity
In all of the participants, positive correlations between volume and ipsilateral structural connectivity (sum of the number of connecting fibers) were observed in the bilateral hippocampi, with R = 0.20 (P < 0.05, Figure 4A) on the left and R = 0.28 (P < 0.01, Figure 4B) on the right. In contrast, in every group, positive correlations between hippocampal volume and its structural connectivity were only present in the NC group, with R = 0.42 (P < 0.01) on the left and R = 0.64 (P < 0.001) on the right.
Figure 4
Classification Performance of Hippocampal Volume and Structural Connectivity
Among the AD, aMCI, and NC groups, the classification power of bilateral hippocampal volume, structural connectivity (sum of the number of connecting fibers), and their combination were shown by AUC (Table 3 and Figure 5). The AUC values obtained by the combination of hippocampal volume and structural connectivity were all higher than those obtained by a single parameter in every two-group comparison. However, in AD vs. NC, the classification accuracy (ACC) obtained by the combination of hippocampal volume and structural connectivity did not exceed that obtained by hippocampal volume only, whereas, in AD vs. aMCI, aMCI vs. NC, the ACC with two parameters both exceeded that with a single parameter (Table 3).
Table 3
| AD vs. NC | aMCI vs. NC | AD vs. aMCI | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Vol | Con | Com | Vol | Con | Com | Vol | Con | Com | |
| ACC | 77.92% | 72.37% | 76.62% | 60.56% | 43.66% | 67.61% | 63.64% | 57.58% | 68.18% |
| SEN | 69.44% | 55.56% | 75.00% | 43.33% | 6.67% | 46.67% | 44.44% | 47.22% | 52.78% |
| SPE | 85.37% | 87.80% | 78.05% | 73.17% | 70.73% | 82.93% | 86.67% | 70.00% | 86.67% |
| AUC | 0.83 | 0.73 | 0.86 | 0.56 | 0.31 | 0.61 | 0.65 | 0.53 | 0.71 |
Accuracy (ACC) and area under the ROC curve (AUC) by hippocampal volume, structural connectivity (sum of the number of connecting fibers), and their combination in classifying the AD, aMCI, and NC groups.
By combining hippocampal volume and structural connectivity, the AUC values for AD vs. NC, aMCI vs. NC, and AD vs. aMCI were all higher than those by hippocampal volume or structural connectivity separately. Abbreviations: ROC, receiver operating characteristic; Vol, volume; Con, hippocampal structural connectivity (sum of the number of connecting fibers); Com, combination of volume and structural connectivity; SEN, sensitivity; SPE, specificity.
Figure 5
Discussion
AD is widely considered a disconnection syndrome accompanied by disruption of brain structural and functional networks. Although hippocampal atrophy is closely associated with memory decline in AD and aMCI, the alterations in structural connectivity between the hippocampus and whole brain regions remain unclear.
Alterations in Hippocampal Volume and Structural Connectivity
Hippocampal atrophy was present in all slices in AD patients but predominantly in the hippocampal head in aMCI patients (Joko et al., ). Moreover, in patients with AD and those with aMCI converters to AD, the left hippocampus showed maximal atrophy (Filippi et al., ). Consistent with the literature, we also demonstrated atrophy of the bilateral hippocampi in AD and aMCI patients, predominantly on the left.
In addition to the decrease in hippocampal connections with temporal, inferior parietal, posterior cingulate, and frontal regions in AD (Rowley et al., ), nodal efficiency and connections of the hippocampus also revealed a significant decrease in AD patients compared with NC subjects (Wang et al., ). Thus, we further investigated structural connectivity (number of connecting fibers) between the hippocampus and whole-brain regions in AD and aMCI patients. Brain regions containing alternative structural connectivity with the bilateral hippocampi mostly belonged to the DMN. The topological organization of whole-brain structural connectivity was disrupted more severely in aMCI converters to AD than in nonconverters, especially in the DMN regions and connections (Sun et al., ). Our results provide clues regarding the participation of DMN regions in the pathogenesis of AD and aMCI at the local level.
Correlations With Scores of Neuropsychological Tests
The hippocampus plays an indispensable role in episodic memory. Significant associations between hippocampal atrophy and autobiographical memory loss, naming, and executive dysfunction were identified in patients with AD and aMCI (Nagata et al., ; Choi et al., ; Liechti et al., ; Serra et al., ). Here, in the AD and aMCI groups, MMSE and MoCA scores both revealed positive correlations with bilateral hippocampal volume, especially on the left. However, in each AD, aMCI, and NC group, no significant correlation between neuropsychological tests and hippocampal volume was observed. The correspondence between hippocampal volume and comprehensive cognitive decline in the single AD group or aMCI group was lower but apparent in AD and aMCI patients together.
As reported, FA and MD of the fornix, the predominant efferent tract from the hippocampus to the thalamus, correlated with memory performance (Mielke et al., ), whereas MD of the tract connecting the hippocampus to the PC/PCC correlated with verbal memory in AD (Palesi et al., ). However, for correlations between total cognitive assessments and sum of hippocampal structural connectivity in our data, none was observed in AD and aMCI patients, as well as in single AD, aMCI, and NC group. This may be due to a reduction in the inconformity of number of connecting fibers between the hippocampus and special brain regions. For example, a decrease in structural connectivity between the hippocampus with the thalamus and lingual gyrus showed relative preservation (Figure 2A).
Correlation Between Hippocampal Volume and Structural Connectivity
In the three groups together, we showed positive correlations between bilateral hippocampal volume and ipsilateral structural connectivity (sum of the number of connecting fibers), but in every three groups, we found the above correlations only in NC subjects, with a higher value of relativity. The results imply that connections between hippocampal volume and its structural connectivity are present in normal conditions and can be disrupted when pathology occurs. The absence of these correlations in the AD group or aMCI group may be because of the unapparent differences among each case.
Our results also demonstrate that the reduction in number of connecting fibers is due to hippocampal neuron loss. However, brain disconnection is mainly located in the temporal lobe in preclinical individuals, progresses to the parietal and frontal lobes at the MCI stage, and contains almost all brain regions in AD patients, indicating that WM damage is driven not only by GM atrophy (Tucholka et al., ). The deposition of Aβ was reported to be crucial to structural connectivity alterations (Shigemoto et al., ; Hwang et al., ). The fornix displayed stronger and more focal changes in MCI subjects with neurodegeneration and amyloid positivity than in those only with neurodegeneration positivity but no amyloid positivity (Jacquemont et al., ). Thus, in AD and aMCI cases, a reduction in hippocampal structural connectivity may also result from direct injury by Aβ deposition in WM.
Discrimination by Hippocampal Volume and Structural Connectivity
Early diagnosis of AD and aMCI is essential for early intervention and therapy. MRI-based hippocampal volume is the best-established imaging marker for the prediction of aMCI conversion to AD (Bosco et al., ). Furthermore, multimodal MRI metrics were reported to elevate the discriminating power for the AD, aMCI, and NC groups. For example, a combined ROC curve of hippocampal volume, stiffness, and MD yielded a significantly improved AUC of 0.90 (Gerischer et al., ). Diagnostic significance for mild AD can be improved by a combination of the volume of hippocampal subfields (CA1 and subiculum) and diffusivity parameters (Li et al., ). A combination of hippocampal volume and immediate memory appeared to perform best in predicting conversion time from MCI to AD (Tabatabaei-Jafari et al., ).
Here, we combined hippocampal volume with its structural connectivity as a diagnostic tool and obtained a higher AUC value than that obtained by a single parameter in the discrimination of the AD, aMCI, and NC groups. However, the ACC of AD vs. NC obtained by a combination of the two metrics did not exceed that obtained by hippocampal volume but exceeded that obtained by hippocampal structural connectivity. This implies that in AD subjects, hippocampal volume alterations may be more severe than a reduction in the number of connecting fibers.
Limitations
The main limitation of this study is the lack of CSF biomarkers and/or amyloid PET to confirm the AD diagnosis. Due to the different cultural backgrounds in China, lumbar puncture is difficult for patients and their relatives to accept. Furthermore, no reimbursement for amyloid PET expenses by medical insurance hampered the examinations. Thus, for enrollment of patients with AD, we inevitably relied on the clinical criteria established in 1984 (McKhann et al., ) but not the up-to-date NIA-AA Research Framework from 2018 (Jack et al., ), which offered a biologically based definition of AD.
There are still other limitations in the current study. The discriminative power of the combination of hippocampal volume and its structural connectivity was relatively weak, especially for aMCI vs. NC and AD vs. aMCI. This may be due to the failure of discrimination in hippocampal efferent and afferent fibers by tractography. In this regard, we need to further complement the hippocampus-related parameters for the diagnosis of AD and aMCI. Furthermore, the consistency of functional and structural connectivity reduction in the hippocampus was not evaluated here. However, functional and structural connectivity networks present an overlap disruption in patients with AD (Dai et al., ). Our previous study showed that in AD and aMCI patients, structural connectivity between the left hippocampus and thalamus affects the functional connectivity between them (Feng et al., ). Thus, further study on the consistency of functional and structural connectivity changes is definitely needed.
Conclusion
Here, we demonstrated a positive correlation between hippocampal volume and ipsilateral structural connectivity in AD and aMCI patients. Moreover, a superiority in discrimination for the AD, aMCI, and NC groups was displayed by the combination of hippocampal volume and its structural connectivity, which provides a new point of view for the diagnosis of AD and aMCI. However, to afford more clues for AD and aMCI pathology, relationships between hippocampal volume and its structural connectivity still need further investigation.
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Statements
Data availability statement
The dataset used and analyzed within this article will be made available from the corresponding authors upon reasonable request.
Ethics statement
The studies involving human participants were reviewed and approved by Medical Ethics Committee of the Chinese PLA General Hospital. The patients/participants/legal guardians provided their written informed consent to participate in this study.
Author contributions
FF: investigation, resources, data curation, methodology, formal analysis, writing—original draft, writing—review and editing. WHu: data curation, methodology, formal analysis, software, writing—original draft, writing—review and editing. QM, WHa, HY, YG, and NA: investigation, data curation, writing—review and editing. BZ: investigation, data curation, funding acquisition, writing—review and editing. CZ: investigation, resources, writing—review and editing. LW: resources, writing—review and editing. XH: methodology, project administration, supervision, writing—review and editing. XZ: conceptualization, resources, data curation, methodology, funding acquisition, project administration, supervision, writing—review and editing. NS: conceptualization, data curation, methodology, software, funding acquisition, project administration, supervision, writing—review and editing. All authors contributed to the article and approved the submitted version.
Funding
This study was supported by the National Natural Science Foundation of China (Grant No. 81471120, No. 81671761, and No. 81871425), the Fund Projects in Technology of the Foundation Strengthening Programme (Grant No. 2019-JCJQ-JJ-151), and the Ministry of Science and Technology of the People’s Republic of China (2019YFC0118203).
Acknowledgments
We sincerely thank all of the subjects for their willingness to participate in this study.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
Alzheimer’s disease, amnestic mild cognitive impairment, hippocampus, volume, structural connectivity
Citation
Feng F, Huang W, Meng Q, Hao W, Yao H, Zhou B, Guo Y, Zhao C, An N, Wang L, Huang X, Zhang X and Shu N (2021) Altered Volume and Structural Connectivity of the Hippocampus in Alzheimer’s Disease and Amnestic Mild Cognitive Impairment. Front. Aging Neurosci. 13:705030. doi: 10.3389/fnagi.2021.705030
Received
04 May 2021
Accepted
10 September 2021
Published
05 October 2021
Volume
13 - 2021
Edited by
Claudia Jacova, Pacific University, United States
Reviewed by
Giovanni Bellomo, University of Perugia, Italy; Dong Woo Kang, The Catholic University of Korea, South Korea; Zhengjia Dai, Sun Yat-sen University, China
Updates
Copyright
© 2021 Feng, Huang, Meng, Hao, Yao, Zhou, Guo, Zhao, An, Wang, Huang, Zhang and Shu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Xusheng Huang lewish301@sina.com Xi Zhang zhangxi@301hospital.com.cn Ni Shu nshu@bnu.edu.cn
†These authors have contributed equally to this work
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