- 1Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH, United States
- 2Department of Biomedical Sciences, University Hospitals Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH, United States
Introduction: A major contributor to age-related hearing loss is the decline of GABAergic inhibition, particularly in the inferior colliculus (IC), which is the midbrain hub of the central auditory system. The initial loss of inhibition is thought to be a compensatory mechanism in response to decreased peripheral excitation. However, the downregulation of inhibition in the IC persists with age and leads to functional disruptions and central neural gain. Neuropeptide Y (NPY) is co-expressed by a sub-population of GABAergic IC cells whose age-related changes remain unexplored. We sought to characterize GABAergic cells in the major subdivisions of the IC that express NPY mRNA to determine whether NPY mRNA is altered in aging IC cells.
Methods: We used multiplexed fluorescent in situ hybridization (smFISH) to label lemniscal and non-lemniscal IC cells that express NPY mRNA and/or GAD1 mRNA in four age groups of Fischer Brown Norway (FBN) rats.
Results: The data demonstrate that ∼38% of GABAergic IC cells co-express NPY, the largest proportion of NPY cells is in the non-lemniscal dorsal IC (ICd), the majority of NPY cells have medium profile areas, and the expression of individual NPY mRNA is unaffected by age.
Discussion: While GABAergic inhibition is reduced with age, it appears that NPY driven inhibition may remain intact. GABAergic neurons that co-express NPY may represent a marked population that persists throughout aging, suggesting that they are not the primary contributor to age-related loss of inhibition.
1 Introduction
Age-related hearing loss (ARHL), a commonly reported chronic health condition amongst the elderly population, impacts an individual’s ability to communicate and poses serious health risks (Goman and Lin, 2016; Haile et al., 2021; Man et al., 2021). Diminished ability to hear high frequency sounds, decreased speech understanding, and impaired sound localization are several of the hallmarks of ARHL, which encompass a combination of peripheral and central auditory deficits (Ibrahim and Llano, 2019; Li-Korotky, 2012; Ouda et al., 2015). The central neural gain hypothesis suggests that decreased excitation from the periphery leads to a compensatory downregulation of GABAergic inhibition centrally, particularly in the auditory midbrain (Ono and Oliver, 2014; Ibrahim and Llano, 2019; Knipper et al., 2022; Rumschlag et al., 2022; Ouda et al., 2015). Although advantageous at first, the continued loss of inhibition into old age leads to dysfunction in temporal coding, signal-to-noise ratios, and shaping of acoustic signals (Palombi and Caspary, 1996a,b; Walton et al., 1998, 2002; Frisina, 2001; Suta et al., 2011; Parthasarathy and Bartlett, 2011, Pollak et al., 2011; Cai et al., 2014; Koehler et al., 2023).
The age-related downregulation of GABA and its precursor, glutamic acid decarboxylase (GAD), are well established in the inferior colliculus (IC), which is the midbrain hub of the central auditory system (Wenstrup, 2005; Caspary et al., 2008; Syka, 2020; Caspary et al., 1990, 1995; Burianova et al., 2009; Gutiérrez et al., 1994; Raza et al., 1994; Ouda and Syka, 2012; Rabang et al., 2012; Milbrandt et al., 1994, 1996, 1997; Pal et al., 2019; Koehler et al., 2023). The IC is separated into three anatomical subdivisions across most species: the central (ICc), lateral cortex (IClc), and dorsal cortex (ICd) (see reviews: Oliver, 2005; Syka, 2020). Nearly all major ascending and descending auditory information is processed in the IC: ascending lemniscal information is largely processed in the ICc and IClc, descending cortical information in the ICd and IClc, as well as intrinsic and commissural information across the three subdivisions (González-Hernández et al., 1996; Oliver, 2005; Schofield, 2010; Thompson, 2005; Wenstrup, 2005; Mellott et al., 2014a,2018). The IC is comprised of GABAergic cells, approximately 20%–40% of the population, and glutamatergic cells, making up the remainder of the cell population (Peruzzi et al., 1997; Merchán et al., 2005; Mellott et al., 2014a; Schofield and Beebe, 2019). Functionally, GABAergic IC cells can be placed into loose categories based on extracellular markers and soma size (Ito et al., 2009; Beebe et al., 2016). Recent studies suggest that co-transmitter Neuropeptide Y (NPY) is expressed by a subpopulation of GABAergic neurons in the IC to inhibit local excitation (Silveira et al., 2020, 2023).
Neuropeptide Y is a 36-amino acid residue peptide that is abundant in the brain and is implicated in many physiological functions, including circadian rhythms, neuronal excitability, neuroplasticity, and memory (Gøtzsche and Woldbye, 2016). NPY is primarily expressed by GABAergic neurons, but it has also been observed in astrocytes and in circulation (Edvinsson et al., 1983; Ramamoorthy and Whim, 2008; Silveira et al., 2020). Further characterization has demonstrated that ICc NPY neurons have a stellate morphology and their dendritic arbors reach across isofrequency laminae (Drotos and Roberts, 2024; Goyer et al., 2019; Silveira et al., 2020). NPY neurons project to the contralateral IC and to the medial geniculate body (MG) of the auditory thalamus (Nakagawa et al., 1995; Silveira et al., 2020). NPY neurons represent approximately one third of the GABAergic cells in the mouse IC and the NPY Y1 receptor has been shown to be expressed by 80% of glutamatergic cells in the IC (Silveira et al., 2023). It remains to be determined whether NPY is expressed by small, medium, or large GABAergic cells. This is of interest because GABAergic IC cells have been shown to have distinct functional roles based on cell size and neurochemical profile (Beebe et al., 2016; Drotos and Roberts, 2024). Additionally, GABA and its mRNA precursor, GAD1, are differentially downregulated with age, but the age-related changes in NPY remain to be known (Wenstrup, 2005; Caspary et al., 2008; Syka, 2020; Caspary et al., 1990; Burianova et al., 2009; Ouda and Syka, 2012; Rabang et al., 2012; Koehler et al., 2023).
In this study we had two goals: (1) characterize cells that express NPY mRNA in a model commonly used in aging studies, and (2) to determine if NPY mRNA is altered during aging. We used fluorescent small molecule in situ hybridization (smFISH) and fluorescent Nissl staining to investigate age-related changes in the density of NPY cells, their size, and mRNA counts across the aging IC of the Fischer Brown Norway (FBN) rat of four age groups (2–3, 19, 24, and 28 months). Based on previous studies showing downregulation of inhibition in the aging IC, we hypothesized that NPY mRNA would decline with age. We investigated labeling for NPY mRNA, GAD1 mRNA, Neurotrace, and DAPI. Multiplexing for NPY and GAD1 allowed us to determine the distribution and percentage of GABAergic IC cells that co-express NPY. We found that (1) all subdivisions at each age group had many cells that express NPY, (2) the largest proportion of NPY expressing cells were found in the ICd, (3) a little over a third of GAD1 cells co-expressed NPY, (4) most NPY cell profiles were medium sized in area, which persisted throughout age, and (5) the density of mRNA puncta was commonly greater in the ICd across most age groups.
2 Materials and methods
2.1 Animals
All procedures were conducted in accordance with the Northeast Ohio Medical University Institutional Animal Care and Use Committee and NIH guidelines (IUCUC protocol number: #23-01-351). Results are described from 23 male Fischer Brown Norway (FBN) rats (National Institute of Aging; Bethesda, MD, United States; RRID:SCR_007317) across four age groups: 2–3 months “young” (eight animals); 19 months “early middle-age” (five animals); 24 months “late middle-age”(five animals); 28 months “old” (five animals; Table 1). Efforts were made to minimize the number of animals and their suffering.
2.2 Perfusion and sectioning
Each animal was deeply anesthetized with isoflurane and perfused transcardially with 0.9% saline or Tyrode’s solution buffered at a pH of 7.4. The brain was removed, the frontal cortex was “blocked,” placed in a plastic cube mold, covered in optimal cutting temperature compound (OCT), flash frozen in liquid nitrogen, and stored at −80°C. The brain was then removed from the −80°C storage, the plastic mold was removed, and the cube of OCT/brain was placed in a cryostat (Leica) for 30–60 min to warm up to −20°C for sectioning. The brain was cut into 12 μm thick transverse sections and collected on Superfrost Plus slides (Fisher Scientific) and placed back into −80°C storage until reacted for single molecule fluorescent in situ hybridization (smFISH).
2.3 Tissue processing for smFISH
Mid-rostrocaudal inferior colliculus (IC) sections (between interaural levels 0.24–0.48 mm; Paxinos and Watson, 1998) were chosen in which the three major IC subdivisions [central IC (ICc), lateral cortex of the IC (IClc) and dorsal cortex of the IC (ICd)] were present. IC sections were processed for Neuropeptide Y [NPY; targeted bases 2-564, Cat. No. 1211911-C1, Advanced Cell Diagnostics (ACD), Unite States] and glutamate decarboxylase 1 (GAD1; targeted bases 62-3113, Cat. No. 316401-C2, ACD, Unite States) and a blank negative control (Cat. No. 320891, ACD, Unite States) using the Fluorescent Multiplex Reagent v2 Kit according to the manufacturer’s instructions (Cat. No. 323137, ACD, Unite States) as in Salehi et al. (2018), Mellott et al. (2022), Koehler et al. (2023). Briefly, slides with IC sections were immersed in a bath of chilled (4°C) 4% paraformaldehyde for 1 h. Tissue was then dehydrated at room temperature, stepwise from 50% to 100% with ethanol. Tissue was airdried and then a hydrophobic barrier was applied around each section. Next, RNAscope Hydrogen Peroxide was applied for 10 min at room temperature and then removed, followed by RNAscope Protease IV applied for 30 min at room temperature and then removed. The NPY and GAD1 probes were combined and added to each section and incubated at 40°C for 2 h. Slides were then submerged in RNAscope Wash Buffer and gently agitated for 2 min. Repeated washes occurred with fresh wash buffer. Development of the NPY and GAD1 mRNA signal occurred through a series of amplification steps at 40°C: Amp 1, 30 min; Amp 2, 30 min; Amp 3, 15 min. All steps at 40°C were conducted within a controlled humidity environment (HybEZ oven and humidity paper, ACD, United States) such that sections never dried out. RNAscope Horse Radish Peroxidase (HRP) was applied for 15 min at 40°C. Fluorescent opal reagent packs were then applied to each section to label NPY and GAD1 mRNA (NPY; Opal 650, Cat. No. FP1497001KT, Akoya Biosciences, United States; GAD1: Opal 780 reagent pack, Cat. No. FP1501001KT, Akoya Biosciences, United States). Sections were then washed and counter stained for DAPI and a green (500/525) Nissl stain, NeuroTrace (NT, Molecular probes, diluted 1:100), to label nuclei and cell bodies, respectively.
2.4 Data analysis and puncta detection
Two quadruple-labeled transverse IC sections were used for quantification per case. Each section was outlined using a Neurolucida reconstruction system (MBF Bioscience, Williston, VT, United States) attached to a Zeiss AxioImager M2 fluorescence microscope (Carl Zeiss) and Hamamatsu Orca Flash 4.0 camera (Hamamatsu). Differential patterns of neural packing density and somatic size, determined by Nissl, and the rat anatomical atlas of the brain were used to draw borders between the central nucleus (ICc) and the lateral (IClc) and dorsal (ICd) cortices of the IC (Faye-Lund and Osen, 1985; Loftus et al., 2008, Mafi et al., 2022). We initially subdivided (with Neurolucida) the ventromedial-dorsolateral axis of the ICc into three equal lengths to create anatomical regions representing the distribution of high, middle, and low frequencies. However, analysis showed no significance across these three regions regarding age-related changes in NPY mRNA puncta counts. Consistent with previous studies of NPY in the IC, we found NPY+ cell bodies in all three subdivisions of the IC (Nakagawa et al., 1995; Silveira et al., 2020, 2023).
One IC section per case was used for plotting NPY+/GAD1+ (12,546 cells were quantified), GAD1+ only (20,712 cells were quantified), and NPY+ only (979 cells were quantified). Each combination was plotted with a unique marker. Every cell that expressed NPY and/or GAD1 mRNA was manually plotted with a 40x objective aligned to a Neurolucida reconstruction system (MBF Bioscience, Williston, VT, United States) attached to a Zeiss AxioImager M2. Plots of markers and IC subdivisions were then analyzed through NeuroExplorer (MBF Bioscience, Williston, VT, United States).
One IC section per case was chosen for NPY mRNA puncta quantification. Each NPY mRNA expressing cell, with a visible nucleus was contoured within Neurolucida to obtain a profile area (Figure 1). Profile area categories for this study were determined by our previous study on GAD1 mRNA (Koehler et al., 2023). Briefly, profile areas were log transformed in R to establish a quantile distribution of the lower, middle and upper third data sets that define our small (< 124 μm2), medium (124–411 μm2) and large (> 411 μm2) profile sizes (Koehler et al., 2023).

Figure 1. Quantification of Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) puncta in inferior colliculus (IC) cells. High magnification photomicrographs showing cells expressing NPY mRNA (magenta) and glutamate decarboxylase 1 (GAD1) mRNA (cyan) in the ICd of a middle aged (19 months) animal. At 150× magnification the individual mRNA signals are optically resolved. (A) Each magenta signal represents a single mRNA for NPY. (B) Same cells imaged to show GAD1 mRNA expression. (C) Merged image showing colocalization of NPY mRNA and GAD1 mRNA in each cell. DAPI (yellow) counterstain. (D) Plot of the same cells. White contours outline the somatic profile area of each cell (profile areas: 278.6 μm2, left cell; 333.9 μm2, right cell). Profile area was determined by counterstaining the tissue with NeuroTrace (not shown). Each purple puncta represents a single mRNA for NPY. Scale bar = 25 μm.
Neuropeptide Y mRNA puncta was manually plotted (Neurolucida) with a 150x glycerine-immersion objective (Zeiss) for each contoured cell (Figure 1D). A total of 13,452 NPY cells were contoured and had their mRNA plotted. Plots of contoured cells, mRNA puncta, and IC subdivisions were then analyzed through NeuroExplorer (MBF Bioscience, Williston, VT, United States). All photomicrographs presented here are 2–7 μm (0.2–0.5 μm steps) maximum image projections and were captured with a Zeiss AxioImager M2 fluorescence microscope and Hamamatsu Orca Flash 4.0 camera (Hamamatsu). Adobe Photoshop (Adobe Systems) was used to add scale bars, crop images, and adjust intensity levels. The results of these plots were used for a quantitative summary of the NPY mRNA across different cell sizes in different IC subdivisions, and across four age groups. Final images of the plots were refined with Adobe Illustrator (Adobe Systems, Inc., San Jose, CA, United States) for preparation of figures.
Variation in the density of mRNA, NPY+, GAD+, and GAD+/NPY+ cells was assessed using mixed-effects analyses of variance (ANOVAs), specifying age group and IC regions (nested within individual) as fixed factors, and individual animal as a random intercept in all models (see Supplementary Tables 1–4). Mixed-effects models permitted us to control for the potential pseudoreplication that would arise from analyzing repeated measurements from the same individuals (Harrison et al., 2018). To better approximate distributional normality, as assessed using quantile-quantile (QQ) plots (Sokal and Rohlf, 2011), mRNA density was square root transformed and NPY+, GAD+, and NPY/GAD+ densities were rank-transformed prior to analysis. We fit full-factorial models for each variable of interest, testing the main effect of age, the main effect of IC region, and the interaction between age and IC region. The accuracy of all mixed-effects model fits was assessed by (1) visual inspection of residual-vs-fitted value plots and (2) use of QQ plots to verify distributional normality of model residuals. Pairwise post hoc comparisons between age groups within IC regions or between IC regions within age groups were corrected for alpha-inflation using the False Discovery Rate method (Benjamini and Hochberg, 1995).
Significance for all tests was accepted at p < 0.05. All statistical analyses were conducted in the R statistical platform v. 4.4.2 “Pile of Leaves” (R Core Team, 2025), including the add-on packages emmeans (Lenth, 2024), lme4 (Bates et al., 2015), lmerTest (Kuznetsova et al., 2017), and tidyverse (Wickham et al., 2019).
3 Results
3.1 NPY mRNA is expressed in all three subdivisions of the IC in FBN rats
To investigate the distribution of GABAergic cells that express NPY in the FBN rat IC, we performed smFISH for NPY mRNA and GAD1 mRNA. As shown in Figure 2A, NPY + mRNA puncta were observed throughout the whole IC, with a denser distribution throughout the ICd (Figure 2A). GAD1 + mRNA puncta were diffusely distributed throughout the ICd, IClc, and ICc (Figure 2B). Even from a low magnification, it was obvious that most cells expressing NPY also expressed GAD1 (Figure 2C). Overall, a total of 12,546 NPY/GAD1 cells were plotted, 20,712 GAD1 only cells were plotted, and we found that 979 cells expressed NPY without GAD1. Thus, 37.7% (12,546/33,258) of GAD1 cells also expressed NPY.

Figure 2. Fluorescent in situ hybridization for Neuropeptide Y (NPY) and glutamate decarboxylase 1 (GAD1) messenger ribonucleic acid (mRNA). NPY mRNA is expressed in all subdivisions of the inferior colliculus (IC). (A) Low magnification image of NPY mRNA (magenta) expression in the ICc, ICd, and IClc. (B) Expression of GAD1 mRNA (cyan) in the ICc, ICd, and IClc. (C) Merged image showing differential expression of NPY mRNA as compared to GAD1 mRNA across the IC. Scale bar = 1 mm.
Representative schematic plots of cells that either express both NPY and GAD1 mRNA (purple circles), only GAD1 mRNA (cyan circles), or only NPY mRNA (pink circles) in the subdivisions of the FBN rat IC at 2–3, 19, 24, and 28 months (Figure 3). Regardless of age, the ICd had the highest (∼> 60%) proportion of cells that expressed both NPY/GAD1 (Figure 3). The lowest (∼< 35%) proportion of co-expressing cells was commonly in IClc (Figure 3). The proportion of co-expressing cells in the ICc fell in the middle (Figure 3). We then examined the density of these co-expressing cells within the known area (μm2) of each subdivision across all animals (Figure 4). Regardless of aging, the density of cells that co-express NPY and GAD1 was significantly higher in the ICd as compared to the ICc (***p < 0.0001) and the IClc (***p < 0.0001; Figure 4). Density was higher (*p = 0.0141) in the ICc than the IClc (Figure 4).

Figure 3. Plots showing the distribution of inferior colliculus (IC) cells that co-express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) and glutamate decarboxylase 1 (GAD1) mRNA (purple circles), only express NPY mRNA (pink circles), or only express GAD1 mRNA (blue circles) across four age groups. Percentage of IC cells that co-express NPY mRNA and GAD1 mRNA for the individual subdivisions are shown in parentheses. Plotted sections were chosen between interaural levels 0.12–0.48 mm; (Paxinos and Watson, 1998). D, dorsal; L, lateral. Transverse sections at a mid-rostrocaudal level of the IC. Scale bar = 1 mm.

Figure 4. Variation in NPY+/GAD1+ density as a function of age and inferior colliculus (IC) subdivision [i.e., central (ICc), dorsal cortex (ICd), or lateral cortex (IClc)]. Raw data were rank-transformed prior to analysis due to departure from distributional normality (Wood and Games, 1990). Small markers indicate measurements of individual animals, with different symbols representing individuals and darker hues indicating older ages, as shown in the legend. Large circles with error bars represent estimated marginal means within each IC subdivision, and 95% confidence intervals around these means, respectively, as predicted by the mixed-effects regression model. Horizontal bars indicate significant differences between groups (*, p < 0.05; ***, p < 0.001).
Representative plots from each age group indicated that cells only expressing GAD1 (cyan) in the ICd only account for 29%–36.2% of the examined population, while in the ICc and IClc GAD1 only cells could account for more than half of the total population (Figure 3). We then examined the density of these GAD1 only expressing cells within the known area (μm2) of each subdivision across all animals (Figure 5). The density of these GAD1 only cells significantly decreased from 2–3 months to 24 months (*p = 0.0216) and to 28 months (*p = 0.0403; Figure 5).

Figure 5. Variation in GAD1+ as a function of age and inferior colliculus (IC) subdivision [i.e., central (ICc), dorsal cortex (ICd), or lateral cortex (IClc)]. Raw data were rank-transformed prior to analysis due to departure from distributional normality (Wood and Games, 1990). Small markers indicate measurements of individual animals, with different symbols representing individuals. Large circles with error bars represent the estimated marginal means at each age, and 95% confidence intervals around these means, respectively, as predicted by the mixed-effects regression model. Horizontal bars indicate significant differences between groups (*, p < 0.05).
Representative plots from each age group indicated that cells expressing only NPY (pink) were not common in the ICd (1.8%–5.2%), the ICc (3.4%–7.1%), and the IClc (0.7%–4.6%; Figure 3). We then examined the density of these NPY only expressing cells within the known area (μm2) of each subdivision across all animals (Figure 6). However, there were no significant differences noted in their expression patterns (Figure 6).

Figure 6. Variation in NPY+ density as a function of age and IC subdivision [i.e., central (ICc), dorsal cortex (ICd), or lateral cortex (IClc)]. Raw data were rank-transformed prior to analysis due to departure from distributional normality (Wood and Games, 1990). Small markers indicate measurements of individual animals, with different symbols representing individuals and darker sha. Large circles with error bars represent estimated marginal means at each age, and 95% confidence intervals around these means, respectively, as predicted by the mixed-effects regression model.
3.2 NPY and GAD1 mRNA is co-expressed in small, medium, and large IC cells
Following our previous study on GAD1 mRNA (Koehler et al., 2023) we recognized three size groups: small cells have a profile area that is less than 124 μm2, medium cell profile areas ranged from 124 to 411 μm2, and large cells have profile areas greater than 411 μm2. As only GABAergic cells express NPY in the IC, we apply these profile areas to our NPY cells. In the current study, the vast majority (79%) of NPY mRNA expressing cells had a profile area between 124 and 411 μm2, thus we defined them as medium. The remaining 21% was split across small (10.8%) and large (10.2%) populations. When examining specific IC subdivisions, medium NPY cells were the most common size in each subdivision at each age (Table 1). The overall average profile area between ages ranged from 218.68 to 301.37 μm2 (Table 1). Interestingly, the average profile area at each age was that of a medium cell, although averages were larger during 19 and 24 months (Table 1).
Multiplexing smFISH techniques show specific examples of small, medium and large GABAergic cells that express NPY mRNA (Figure 7). Small cells (top cell, 119.6 μm2; middle cell, 116.5 μm2; bottom cell, 69.8 μm2) that co-express NPY and GAD1 mRNA can be readily identified in the ICd (white arrowheads; Figures 7A–C). Medium cells (top cell, 233.4 μm2; bottom cell, 365.6 μm2) that co-express NPY and GAD1 mRNA in the ICd (white arrowheads; Figures 7D–F). A large cell (587.4 μm2) that co-expresses NPY and GAD1 mRNA (white arrowhead; Figures 7G–I). Mixed-effect modeling did not find a significant difference of the distribution of NPY profile areas across subdivisions or ages.

Figure 7. Cells that express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) vary in soma profile area. High magnification (150×) photomicrographs showing NPY mRNA expression in small, medium, and large cells, based on soma size classification of GAD1+ cells in previous studies (small, < 124 μm2; medium, 124–411 μm2; large, > 411 μm2). NPY mRNA is shown in magenta, glutamate decarboxylase 1 (GAD1) mRNA is shown in cyan, and DAPI is shown in yellow. Each row represents a single cell size. (A–C) Photomicrographs of small cells (arrowheads) in the ICd of a young (3 months) animal (top cell, 119.6 μm2; middle cell, 116.5 μm2; bottom cell, 69.8 μm2). (D–F) Photomicrographs of medium cells (arrowheads) in the ICd of a young (3 months) animal (top cell, 233.4 μm2; bottom cell, 365.6 μm2). (G–I) Photomicrographs of large cells (arrowheads) in the ICc of a young (3 months) animal (center cell, 587.4 μm2). Scale bar = 25 μm.
3.3 Expression of NPY mRNA in 2–3 months old FBN rats
We manually quantified each NPY mRNA across each age and IC subdivision (Figure 1 and Table 2). Figure 8 shows representative photomicrographs across the ICc, ICd, and IClc at 2–3 months of cells that express both NPY and GAD1 (white arrowheads) and GAD1 cells that do not express NPY mRNA (white arrows). Small cells had an average of 40.7 mRNA puncta in the ICc, 41.7 mRNA puncta in the ICd, and 35.5 mRNA puncta in the IClc (Table 1). Medium cells had an average of 80.3 mRNA puncta in the ICc, 92.7 mRNA puncta in the ICd, and 84.3 mRNA puncta in the IClc (Table 1). Large cells had an average of 173.7 mRNA puncta in the ICc, 222.5 mRNA puncta in the ICd, and 183.6 mRNA puncta in the IClc (Table 1).

Table 2. Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) across small, medium, and large inferior colliculus (IC) cells.

Figure 8. Structured illumination fluorescence images, taken at 0.2 μm steps, showing cells that co-express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) and glutamate decarboxylase 1 (GAD1) mRNA (arrowheads) and cells that only express GAD1 mRNA (arrows) in a young (3 months) animal. NPY mRNA is shown in magenta, GAD1 mRNA is shown in cyan, and DAPI is shown in yellow. Each row represents a subdivision of the IC. (A–C) Photomicrographs from the ICc. (D–F) Photomicrographs from the ICd. (G–I) Photomicrographs from the IClc. Scale bar = 100 μm.
3.4 Expression of NPY mRNA in 19 months old FBN rats
Figure 9 shows representative photomicrographs across the ICc, ICd, and IClc at 19 months of cells that express both NPY and GAD1 (white arrowheads) and GAD1 cells that do not express NPY mRNA (white arrows). Small cells had an average of 43.2 mRNA puncta in the ICc, 47.3 mRNA puncta in the ICd, and 37.9 mRNA puncta in the IClc (Table 1). Medium cells had an average of 86.9 mRNA puncta in the ICc, 89.8 mRNA puncta in the ICd, and 84.4 mRNA puncta in the IClc (Table 1). Large cells had an average of 181.5 mRNA puncta in the ICc, 230.1 mRNA puncta in the ICd, and 166.2 mRNA puncta in the IClc (Table 1).

Figure 9. Structured illumination fluorescence images, taken at 0.2 μm steps, showing cells that co-express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) and glutamate decarboxylase 1 (GAD1) mRNA (arrowheads) and cells that only express GAD1 mRNA (arrows) in an early middle age (19 months) animal. NPY mRNA is shown in magenta, GAD1 mRNA is shown in cyan, and DAPI is shown in yellow. Each row represents a subdivision of the IC. (A–C) Photomicrographs from the ICc. (D–F) Photomicrographs from the ICd. (G–I) Photomicrographs from the IClc. Red circle depicts a cell that is expressing NPY mRNA only. Scale bar = 100 μm.
3.5 Expression of NPY mRNA in 24 months old FBN rats
Figure 10 shows representative photomicrographs across the ICc, ICd, and IClc at 24 months of cells that express both NPY and GAD1 (white arrowheads) and GAD1 cells that do not express NPY mRNA (white arrows). Small cells had an average of 38.1 mRNA puncta in the ICc, 49.1 mRNA puncta in the ICd, and 48.9 mRNA puncta in the IClc (Table 1). Medium cells had an average of 86.4 mRNA puncta in the ICc, 99.1 mRNA puncta in the ICd, and 109.8 mRNA puncta in the IClc (Table 1). Large cells had an average of 183.7 mRNA puncta in the ICc, 183.7 mRNA puncta in the ICd, and 212 mRNA puncta in the IClc (Table 1).

Figure 10. Structured illumination fluorescence images, taken at 0.2 μm steps, showing cells that co-express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) and glutamate decarboxylase 1 (GAD1) mRNA (arrowheads) and cells that only express GAD1 mRNA (arrows) in a late middle age (24 months) animal. NPY mRNA is shown in magenta, GAD1 mRNA is shown in cyan, and DAPI is shown in yellow. Each row represents a subdivision of the IC. (A–C) Photomicrographs from the ICc. (D–F) Photomicrographs from the ICd. (G–I) Photomicrographs from the IClc. Scale bar = 100 μm.
3.6 Expression of NPY mRNA in 28–29 months old FBN rats
Figure 11 shows representative photomicrographs across the ICc, ICd, and IClc at 28 months of cells that express both NPY and GAD1 (white arrowheads) and GAD1 cells that do not express NPY mRNA (white arrows). Small cells had an average of 55.2 mRNA puncta in the ICc, 57.7 mRNA puncta in the ICd, and 51.4 mRNA puncta in the IClc (Table 1). Medium cells had an average of 67.8 mRNA puncta in the ICc, 72.6 mRNA puncta in the ICd, and 69 mRNA puncta in the IClc (Table 1). Large cells had an average of 143.5 mRNA puncta in the ICc, 183.3 mRNA puncta in the ICd, and 136.5 mRNA puncta in the IClc (Table 1).

Figure 11. Structured illumination fluorescence images, taken at 0.2 μm steps, showing cells that co-express Neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) and glutamate decarboxylase 1 (GAD1) mRNA (arrowheads) and cells that only express GAD1 mRNA (arrows) in an old (28 months) animal. NPY mRNA is shown in magenta, GAD1 mRNA is shown in cyan, and DAPI is shown in yellow. Each row represents a subdivision of the IC. (A–C) Photomicrographs from the ICc. (D–F) Photomicrographs from the ICd. (G–I) Photomicrographs from the IClc. Scale bar = 100 μm.
3.7 NPY mRNA density per cellular profile area across age and IC subdivision
Lastly, pairwise comparisons between subdivisions at each age were computed to examine the density of NPY mRNA per cellular profile area (μm2). At 2–3 months ICd had a greater density of NPY mRNA than both ICc (**p = 0.0021) and IClc (*p = 0.0238; Figure 12). No significant difference in mRNA density was found between ICc and IClc at 2–3 months (p = 0.2938; Figure 12). At 19 months ICd had a greater density of NPY mRNA than IClc (**p = 0.0023; Figure 12). Although not quite reaching statistical significance, there was a trend showing greater mRNA density in ICd than ICc at 19 months (p = 0.0567). No significant difference in mRNA density was found between ICc and IClc at 19 months (p = 0.1072). At 24 months of age the density of NPY mRNA was significantly higher in the ICd than the ICc (***p = 0.0005; Figure 12), and higher in the IClc than the ICc (***p = 0.0009; Figure 12). No significance was found between ICd and IClc (p = 0.7854). There were no significant pairwise differences in NPY mRNA density among IC subdivisions at 28 months (all p ≥ 0.2352). Finally, pairwise comparisons between ages, within subdivisions, demonstrated no significant changes to NPY mRNA density due to age (Figure 12). We conclude that aging is not a factor that affects NPY mRNA density in the IC, though repeating this study with a larger sample size, and thus increased statistical power, may discern significant age related effects.

Figure 12. Variation in messenger ribonucleic acid (mRNA) density as a function of age and inferior colliculus (IC) subdivision [i.e., central (ICc), dorsal cortex (ICd), or lateral cortex (IClc)]. Raw data were log-transformed prior to analysis to improve distributional normality. Small circles indicate replicate measurements of individual animals. Large circles with error bars represent estimated marginal means within each age group and IC subdivision, and 95% confidence intervals around these means, respectively, as predicted by the mixed-effects regression model. For both small and large circles, darker colors indicated older ages, as shown in the legend. Horizontal bars indicate significant differences between ages within groups (= p < 0.1; *, p < 0.05; **, p < 0.01; ***, p < 0.001).
4 Discussion
The current study qualitatively and quantitatively describes NPY cell density and mRNA counts in the aging IC. Our findings demonstrate that NPY is expressed in all three subdivisions of the FBN rat IC, which agrees with previous studies conducted in other rat strains and mouse (Nakagawa et al., 1995; Silveira et al., 2020, 2023). We show that ∼38% of GABAergic IC cells produce NPY mRNA with the greatest proportion (> 60%) occurring in the ICd. Additionally, we demonstrate that 79% of NPY cells have medium sized profile areas. Perhaps the most interesting finding is that the ICd had a greater density of NPY mRNA per cellular profile area, as compared to the ICc and IClc, in our 2–3, 19, and 24 months old animals. However, at 28 months, there was no significant difference in NPY mRNA density between any of the IC subdivisions. Although we hypothesized that NPY mRNA is downregulated with age, our data did not find a significant reduction in mRNA due to aging. Thus, while inhibition declines with age, it appears that NPY driven inhibition may stay intact. NPY-expressing GABAergic neurons may represent a robust population that persists well into old age, suggesting that they are not the primary contributor to age-related loss of inhibition and central neural gain.
4.1 Technical considerations
The National Institute of Aging has recommended the FBN rat as a preferred model of aging because of its long median lifespan, as compared to other strains of mice and rats (Lipman et al., 1996; Lipman, 1997). As such, the FBN rat is commonly used in aging studies of the central auditory system (Caspary et al., 2008; Caspary and Llano, 2018; Cai et al., 2018; Robinson et al., 2019; Mafi et al., 2020, 2021, 2022; Kommajosyula et al., 2021). In this study we use four age groups. Our 2–3 months group equates to the standard “young” age group, when there are no hearing deficits, and our 28 months group is the standard “old” age group, when hearing loss is prevalent. Hearing deficits are uncommon in the 19 months middle age group, while hearing deficits are common in the 24 months middle age group (Cai et al., 2018). However, we acknowledge that a weakness in the study is that these rats did not undergo any physiological characterization or auditory brainstem responses, thus we ultimately do not know if our rats did or did not have any hearing loss at a given age.
As in our previous study, the current study does not rely on aldehyde fixation when extracting and sectioning the brain (Koehler et al., 2023). As mentioned in the Methods, when establishing profile area size for GABAergic cells in the IC for our smFISH studies, profile areas were log transformed in R to establish a quantile distribution that define our small (< 124 μm2), medium (124–411 μm2) and large (> 411 μm2) profile sizes. Previous studies classify medium-sized GABAergic IC cells as having profiles areas up to 300 μm2 (Beebe et al., 2016, 2018). Our higher top-end values (411 vs. 300 μm2) may be due to the lack of fixation and thus less overall shrinkage that is common in standard immunohistochemistry techniques. Also, the tissue for this study was sectioned at 12 μm which may result in a less accurate profile area than studies sectioning at 40–50 μm due to an increased z-axis to examine.
To label GABAergic IC cells we used a GAD1 mRNA probe for the FBN rat as we have previously had success with this gene and GAD1 is a commonly used marker to identify GABAergic cell populations (Dong et al., 2009; Koehler et al., 2023). As our smFISH multiplexing only utilized probes for NPY and GAD1 mRNA, we suggest that the small population of NPY cells we found that did not co-express GAD1, may express GAD2. The IC contains both GAD65- and GAD67- immunoreactive neurons, whose precursors are GAD2 and GAD1, respectively (Burianova et al., 2009; Harding et al., 2024). If NPY in the IC is only co-expressed in GABAergic cells we presume that the cells expressing only NPY mRNA may express genes for GAD2 and therefore may be GABAergic as well. As GABAergic cells throughout the brain may express GAD1 and GAD2, our “NPY-only” cells may simply be a result of the smFISH technique not labeling all mRNA. However, further experiments exploring this distinction would be needed.
4.2 Comparison to previous studies
Our data are consistent with knowledge that NPY in the brain is co-expressed by GABAergic cell populations (Sperk et al., 2007; Ramamoorthy et al., 2011; Aoki and Pickel, 1990; Lall and Biello, 2003; Parker et al., 1998; Kozicz and Lázár, 2001). This relationship is also apparent in the IC as GABAergic cells co-express NPY (Silveira et al., 2020, 2023; Anair et al., 2022). Specifically, previous studies and the current study have focused on the subset of GABAergic principal neurons that co-express NPY (Silveira et al., 2020, 2023). In these studies of the mouse, roughly one-third of the GABAergic IC cells also expressed NPY (Silveira et al., 2020). Our data are consistent with these studies as we found ∼38% of FBN rat GABAergic IC cells also express NPY. Unlike GABAergic IC neurons which are commonly dispersed evenly throughout much of the IC (Oliver et al., 1994; Winer et al., 1995, 1996; Oliver, 2005), we found a non-uniform distribution of NPY expressing cells in the IC such that the ICd had the largest proportion of NPY cells and the largest population of GABAergic cells co-expressing NPY. This finding agrees with Nakagawa et al., 1995, as they also report NPY expression being most robust in the dorsal IC.
GABAergic IC cells are classically characterized as small, medium, or large based on soma size (Ono et al., 2005; Ito et al., 2009; Altschuler et al., 2008; Roberts and Ribak, 1987a,b; Oliver, 2005; Beebe et al., 2016; Koehler et al., 2023). In the current study we defined the vast majority (∼80%) of NPY positive cells as medium-sized. GABAergic cell size has been linked to the intrinsic patterns and projections of the cells (Ono et al., 2005; Ito et al., 2009; Geis and Borst, 2013; Beebe et al., 2016, 2018). Medium-sized GABAergic neurons participate in several long range projections, including outputs to the medial geniculate body (MG), commissural projections to the contralateral IC, and projections to the superior colliculus (SC) (Winer et al., 1996; Oliver, 2005; Mellott et al., 2018, 2019). In the lemniscal IC, cell types are commonly categorized as either disk/flat or stellate/less-flat (Oliver, 2005; Mellott et al., 2014b; Beebe et al., 2016). Also, a more recent study identified basket cell morphologies with distinct physiological profiles in the lemniscal IC (Wallace et al., 2021). Very little is understood about the morphological cell types throughout the non-lemniscal IC. In Silveira (’20) it was demonstrated in the mouse that all NPY cells they had recorded from maintained a stellate morphology regardless of IC subdivision. If NPY cell type is consistent across species, we would suggest that NPY inhibition in the FBN rat is largely driven by medium-sized stellate cells. Previous studies have demonstrated that GABAergic cells can be categorized by the presence of perineuronal nets and/or dense VGlut2-containing inputs (Ito et al., 2009; Beebe et al., 2016, 2018). The current study suggests that NPY cells are “medium-sized and thus may or may not be surrounded by perineuronal nets and/or VGlut2 rings (Beebe et al., 2016, 2018). However, these previous studies used fixed tissue to evaluate their cells size, while in the current approach the cells are unfixed at tissue extraction which results in slight larger average due to a lack of shrinkage (Koehler et al., 2023). It will be interesting for further studies co-labeling NPY, VGlut2, and perineural nets to illuminate these points, and thus provide a greater functional context of IC NPY cells.
A major goal of the current study was to determine if NPY mRNA was downregulated in the aging IC of the FBN rat in the same manner as our previous report on GAD1 (Koehler et al., 2023). Briefly, in our previous work GAD1 mRNA was downregulated with age in smaller GABAergic cells in the lower frequency region, upregulated in larger GABAergic cells, and generally unchanged in medium sized cells between young and old age groups (Koehler et al., 2023). As we found NPY to be most commonly expressed in medium sized cells, collectively it appears that medium sized GABAergic cells in the IC do not undergo robust age-related changed to their mRNA for major inhibitory neurotransmitters. In the current study, although fewer in number (∼20%) NPY mRNA in larger and smaller cells was also unchanged with age. We conclude that it is likely that an individual GABAergic cell can downregulate mRNA for one inhibitory gene (GAD1) while another (NPY) is unaffected by age.
4.3 Functional significance
It is only recently that we have gained a better understanding of the role of NPY in the IC as it has been demonstrated that NPY can inhibit local recurrent excitation (via the Y1 receptor) (Silveira et al., 2023). Additionally, NPY IC cells may provide lateral inhibition through wide-spread heterotopic commissural inputs (Anair et al., 2022). As NPY cell populations are found in each major IC subdivision, one could expect that functional properties persist from lemniscal to non-lemniscal circuits. Given that the non-lemniscal IC plays a large role in the integration of multisensory inputs and receives robust descending cortical inputs, it will be critical for future studies to determine the functional role of NPY across these diverse circuits.
Perhaps the most interesting finding from the current study is that NPY mRNA expression is not affected by age in any IC subdivision. It is well-known that GABAergic inhibition is downregulated in the aging IC (Caspary et al., 2008; Syka, 2020). This initial downregulation of GABAergic inhibition is commonly associated with mechanisms to maintain homeostatic balance during peripheral deafferentation (Kotak et al., 2005, Caspary et al., 2008, Rabang et al., 2012; Resnik and Polley, 2017; Parthasarathy and Kujawa, 2018). However, the continued loss of GABAergic inhibition can lead to many deficits including degraded temporal and binaural coding in speech perception, and the over-amplification of sound-evoked neural responses known as “central gain” (Walton et al., 1998, 2002; Frisina and Rajan, 2005 Kotak et al., 2005, Caspary et al., 2008, Walton, 2010; Parthasarathy and Bartlett, 2011 Rabang et al., 2012; Auerbach et al., 2014, 2019; Resnik and Polley, 2017; Parthasarathy and Kujawa, 2018). These mechanisms may be centrally generated and lead to hyperacusis and tinnitus (Auerbach et al., 2014, 2019). We conclude that NPY inhibition in the aging IC likely plays a very distinctive role from GABAergic inhibition. Studies of NPY across the brain suggest that NPY may help preserve function in aging neurons.
Our results suggest that NPY-mediated inhibition in the IC may not be a major contributor to the onset of presbycusis. Thus, it is possible that the functional roles of NPY mentioned above are intact and/or unaffected by aging. Studies of NPY function in the brain heavily imply that NPY may serve an anti-aging role by employing neuroprotective effects, decreasing excitotoxicity, regulating calcium homeostasis, protecting against oxidative stress and mitochondrial dysfunction, and reducing neuroinflammation (see reviews: Botelho and Cavadas, 2015; Li et al., 2020; Chen et al., 2019; Duarte-Neves et al., 2016). Particularly in the hippocampus, studies have demonstrated that NPY prevents intracellular oxidative stress and activates pathways involved in neuroprotection (dos Santos et al., 2013). Additionally, NPY mediates the production of neurotrophins such as brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) (Croce et al., 2011, 2013; Angelucci et al., 2014). There are noteworthy similarities in NPY expression and function across the IC and hippocampus, including the co-release of NPY and GABA and NPY’s widespread effects on glutamatergic neurotransmission via the Y1 receptor (Sperk et al., 2007; Silveira et al., 2020, 2023). Determining whether various IC functions are preserved via NPY-mediated mechanisms will provide more clarity regarding the aging central auditory system.
The current study indicates that NPY mRNA production is preserved in the aging inferior colliculus, which may signify that NPY helps to maintain function well into old age. We have demonstrated that transcription of NPY mRNA remains intact with age, but further studies are required to determine whether NPY protein translation is also unaffected by aging. Furthermore, future studies are needed to explore the juxtaposition of NPY versus GABA changes with age. Perhaps the persistence of NPY levels in the aging IC serves to provide compensation of function while GABAergic inhibition is being reduced over the lifespan. Additionally, it remains to be determined whether NPY synapses and their postsynaptic contacts are altered with age, similar to GABA (Helfert et al., 1999). And the age-related changes of the NPY Y1 receptor remains, to our knowledge, virtually untouched. Ultimately, the broad role of NPY on hearing at any age requires further exploration.
Data availability statement
The original contributions presented in this study are included in this article/Supplementary material, further inquiries can be directed to the corresponding author.
Ethics statement
The animal study was approved by Northeast Ohio Medical University IACUC. The study was conducted in accordance with the local legislation and institutional requirements.
Author contributions
LA: Formal Analysis, Investigation, Writing – review and editing, Writing – original draft, Data curation, Validation, Conceptualization, Methodology, Project administration, Supervision. KC: Data curation, Writing – review and editing, Investigation. SH: Investigation, Writing – review and editing, Data curation. GS: Writing – review and editing, Investigation, Data curation. GB: Data curation, Investigation, Writing – review and editing. MI: Writing – review and editing, Data curation, Investigation. JH: Investigation, Data curation, Writing – review and editing. NT: Investigation, Writing – review and editing, Data curation. AO: Data curation, Investigation, Writing – review and editing. JY: Methodology, Data curation, Writing – review and editing, Software, Conceptualization, Writing – original draft, Formal Analysis. JM: Project administration, Funding acquisition, Writing – review and editing, Resources, Formal Analysis, Supervision, Writing – original draft, Methodology, Data curation, Investigation, Conceptualization.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by the NIH/NIDCD grant R01 DC017708, and the National Institute on Aging rodent colony.
Acknowledgments
We gratefully acknowledge Dr. Nichole Beebe for critical feedback on an earlier draft of the manuscript.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Generative AI statement
The authors declare that no Generative AI was used in the creation of this manuscript.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnagi.2025.1626021/full#supplementary-material
Abbreviations
FBN, Fischer Brown Norway; NPY, Neuropeptide Y; GABA, gamma-Aminobutyric acid; GAD, glutamic acid decarboxylase; GAD1, glutamate decarboxylase 1; IC, inferior colliculus; ICc, central nucleus of the inferior colliculus; ICd, dorsal cortex of the inferior colliculus; IClc, lateral cortex of the inferior colliculus; mRNA, messenger ribonucleic acid; smFISH, single molecule fluorescent in situ hybridization.
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Keywords: inferior colliculus, NPY, smFISH, NPY mRNA, GAD1 mRNA, aging
Citation: Almassri LS, Crane KM, Hergenrother SR, Singh G, Barach GL, Iafrate MC, Harris JC, Tokar N, Ohl AP, Young JW and Mellott JG (2025) Neuropeptide Y mRNA expression in the aging inferior colliculus of fischer brown norway rats. Front. Aging Neurosci. 17:1626021. doi: 10.3389/fnagi.2025.1626021
Received: 09 May 2025; Accepted: 02 July 2025;
Published: 23 July 2025.
Edited by:
Puliyur Mohan Kumar, University of Georgia, United StatesReviewed by:
Tetsufumi Ito, University of Toyama, JapanMark Lawrence Gabriele, James Madison University, United States
Copyright © 2025 Almassri, Crane, Hergenrother, Singh, Barach, Iafrate, Harris, Tokar, Ohl, Young and Mellott. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jeffrey G. Mellott, am1lbGxvdHRAbmVvbWVkLmVkdQ==