ORIGINAL RESEARCH article
Front. Aging Neurosci.
Sec. Neuroinflammation and Neuropathy
The differential expression profiles of miRNA in serum-derived exosomes and its potential role in age-related hearing loss
Provisionally accepted
Juhong Zhang
Haizhu Ma
Jing KeZiyi Tang
Zhiji ChenGuijun YangLi YangJialin GuoXiaoqi YanChangxiu PengKaiye WangXiyao ChenShaojing Kuang*Wei Yuan*- Department of Intensive Care Medicine, Chongqing General Hospital,, changqing, China
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ABSTRACT Objectives: This study aimed to characterize serum exosomal miRNA profiles from patients with age-related hearing loss (ARHL) to identify key pathogenesis-related miRNAs for ARHL. Methods: Peripheral venous blood samples were collected from patients with ARHL and elderly controls, and exosomes were isolated from serum of each subject. Then, the isolated exosomes were systematically identified by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and western blot. Subsequently, the isolated exosomes were submitted for miRNA sequencing and a series of bioinformatics analysis. Ultimately, four key DE-miRNAs, namely hsa-miR-100-5p, hsa-miR-23b-3p, hsa-miR-373-3p and hsa-miR-27b-3p, were verified using quantitative real-time polymerase chain reaction (RT-qPCR). Results: NTA, TEM and western blot confirmed exosomes were successfully isolated. After sequencing, 22 differential expressed miRNAs (6 up-regulation and 16 down-regulation) were identified between the exosomes from ARHL and controls, and then collectively identified 17,451 predicted target genes and 15,863 experimentally validated target genes. Gene Ontology enrichment analysis revealed that the target genes were significantly associated with "regulation of neuron projection development," "sensory system development," "proteasome-mediated ubiquitin-dependent protein catabolism," and "ubiquitin-like protein ligase binding." Kyoto Encyclopedia of Genes and Genomes (KEGG) showed the target genes were significantly enriched in "PI3K-Akt signaling pathway," "MAPK signaling pathway," "cellular senescence," "autophagy," "mTOR signaling pathway," "ubiquitin-mediated proteolysis," and "signaling pathways regulating stem cell pluripotency." Additionally, the Reactome analysis highlighted the involvement of "MAPK family signaling cascades," "negative regulation of the PI3K/AKT network," and "antigen processing: ubiquitination & proteasome degradation." Disease Ontology further demonstrated significant enrichment of target genes in neurological disorders. RT-qPCR showed
Keywords: age-related hearing loss, Exosomes, miRNA Transcriptome Sequencing, miRNA, Hearing
Received: 28 Aug 2025; Accepted: 27 Nov 2025.
Copyright: © 2025 Zhang, Ma, Ke, Tang, Chen, Yang, Yang, Guo, Yan, Peng, Wang, Chen, Kuang and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Shaojing Kuang
Wei Yuan
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