ORIGINAL RESEARCH article
Front. Genet.
Sec. Cancer Genetics and Oncogenomics
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1509245
This article is part of the Research TopicComparative Genomics and Functional Genomics Analyses in CancerView all 5 articles
Whole Exome Sequencing and Bioinformatics Reveal PMAIP1 and PDGFRL as Immune-Related Gene Markers in Follicular Thyroid Carcinoma
Provisionally accepted
Haobo Wang1
Fangjian Shang1
Xia Jiang2Zhongxin Li2Dongyun Li2Chuanmin Zhou2
Bo Pang2Longfei Kang2Bo Liu1*
Zengren Zhao1*- 1Department of General Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
- 2Gastrointestinal Disease Diagnosis and Treatment Center, Hebei Key Laboratory of Colorectal Cancer Precision Diagnosis and Treatment, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
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The primary clinical challenge associated with follicular thyroid carcinoma (FTC) lies in accurately diagnosing the condition, particularly in distinguishing it with follicular thyroid adenoma (FTA) due to their overlapping cytomorphological features and sonographic characteristics. Whole exome sequencing (WES) techniques and Gene Expression Omnibus (GEO) database were utilized to analyze genomic difference between FTC and FTA, with a specific focus on immune-related genes. The hub genes were subjected to enrichment analysis, immune infiltration analysis, protein-protein interaction (PPI) analysis, and receiver operating characteristic (ROC) curve analysis. Then utilized quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) to validate the expression levels of PMAIP1 and PDGFRL at the cellular and tissue levels.
Keywords: Follicular thyroid carcinoma, Follicular thyroid adenoma, PMAIP1；PDGFRL, whole exome sequencing, bioinformatics
Received: 10 Oct 2024; Accepted: 05 May 2025.
Copyright: © 2025 Wang, Shang, Jiang, Li, Li, Zhou, Pang, Kang, Liu and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Bo Liu, Department of General Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
Zengren Zhao, Department of General Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
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