ORIGINAL RESEARCH article

Front. Genet.

Sec. Genomics of Plants and the Phytoecosystem

Volume 16 - 2025 | doi: 10.3389/fgene.2025.1585510

This article is part of the Research TopicExploring the Genomic Basis of Plant-Microbe Interactions in PhytoecosystemsView all articles

Whole Genome Sequencing and Functional Annotation of Fusarium oxysporum f. sp. lentis to unravel Virulence and Secondary Metabolite biosynthesis gene Clusters

Provisionally accepted
Ashish KumarAshish Kumar1*Sanjay KharteSanjay Kharte1R. Shiv RamakrishnanR. Shiv Ramakrishnan1Stuti SharmaStuti Sharma1Dr. Nishi MishraDr. Nishi Mishra1Priyamvada MishraPriyamvada Mishra2Puneet Singh ChauhanPuneet Singh Chauhan2Radhe Shyam SharmaRadhe Shyam Sharma1Vedant GautamVedant Gautam1Shweta TiwariShweta Tiwari1Vinod GoyalVinod Goyal1Sonu SharmaSonu Sharma1G K KoutuG K Koutu1Neelendra K. JoshiNeelendra K. Joshi3
  • 1Jawaharlal Nehru Agricultural University, Jabalpur, India
  • 2National Botanical Research Institute (CSIR), Lucknow, Uttar Pradesh, India
  • 3University of Arkansas, Fayetteville, North Carolina, United States

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Fusarium oxysporum f. sp. lentis is a major fungal pathogen that causes vascular wilt in lentil crops, leading to significant reductions in yield. Despite its importance, the genetic underpinnings of this pathogen remain poorly understood.We performed whole-genome sequencing of F. oxysporum f. sp. lentis using the Illumina Shotgun Sequencing platform. The resulting high-quality genome assembly consisted of 12,366 contigs with a total length of 124.48 Mb. Genome completeness was evaluated using BUSCO analysis, and functional annotation was performed through comparisons with several public databases, including Uniprot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Pfam, and Clusters of Orthologous Groups (COG). Pathogenicity-related genes were identified using the PHI-base database, and secondary metabolite biosynthesis was analyzed with AntiSMASH.

Keywords: Fusarium oxysporum f. sp. lentis, Illumina shotgun sequencing, pathogenicity, CAZymes, secondary metabolites, Virulence Factors, Host-Pathogen Interactions, gene annotation

Received: 28 Feb 2025; Accepted: 06 May 2025.

Copyright: © 2025 Kumar, Kharte, Ramakrishnan, Sharma, Mishra, Mishra, Chauhan, Sharma, Gautam, Tiwari, Goyal, Sharma, Koutu and Joshi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Ashish Kumar, Jawaharlal Nehru Agricultural University, Jabalpur, India

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