ORIGINAL RESEARCH article
Front. Genet.
Sec. Cancer Genetics and Oncogenomics
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1593553
Integrin factor (FAM27E3), as a metastatic marker of papillary thyroid carcinoma, through the p53 signaling pathway promoting the lymph node metastasis
Provisionally accepted
- 1Yangpu District Kongjiang Hospital, Shanghai, China
- 2Geriatric hospital Affiliated To Wuhan University of Science and Techenology, Wuhan, China
- 3The Sixth Hospital of Wuhan , Affiliated Hospital of Jianghan University, Wuhan, China
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Background: PTC has a lymph node metastasis rate of 20-90%. This study aims to explore the role and regulatory mechanism of the FAM27E3 in PTC lymph node metastasis. Method: The 7 THCA datasets from GEO were combined as the training group, while the PTC dataset served as the testing group. Venn analysis identified overlapping genes through consistent cluster analysis and WGCNA. GO, KEGG, and GSEA analyses were performed to explore the enriched terms. The overlapping genes were entered to the LASSO analysis to identify key influence factors. Finally, FAM27E3 was used for score analysis (EMT, angiogenesis, and mRNAsi scores). Immune cell infiltration was assessed using the CIBERSORT algorithm. CCK8, colony formation, transwell assays, western blotting, and RT-PCR were employed to evaluate cell viability, invasion, migration, and gene/protein expression. Results: The clustering method distinguished the samples into two subtypes. The 128 overlaps were obtained from modules tumor and C1. FAM27E3 was identified as a hub gene associated with PTC lymph node metastasis and had a significantly positive correlation with EMT_score. MRNAsi_score was increased in the FAM27E3 high-expression group. The high expression of FAM27E3 indicated a reduction in the overall macrophage levels. FAM27E3 exhibited high expression in PTC cell lines, and its downregulation suppressed cell proliferation, migration, and invasion. FAM27E3 promoted the lymph node metastasis of PTC, which was associated with p53 signaling pathway. Conclusions: FAM27E3, as a marker for PTC lymph node metastasis, promotes PTC lymph node metastasis related to the p53 signaling pathway.
Keywords: FAM27E3, PTC, lymph node metastasis, p53, macrophage
Received: 20 Mar 2025; Accepted: 07 Jul 2025.
Copyright: © 2025 Zhang, Xiang, Hong, Bao, Wu and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yan Wu, The Sixth Hospital of Wuhan , Affiliated Hospital of Jianghan University, Wuhan, China
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