ORIGINAL RESEARCH article
Front. Genet.
Sec. Genomics of Plants and the Phytoecosystem
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1641368
This article is part of the Research TopicEpigenetic Mechanisms in Plant Adaptation and Stress MemoryView all articles
Mitochondrial genome complexity in Erodium stephanianum (Geraniaceae): nanopore sequencing reveals chloroplast gene transfer and DNA rearrangements
Provisionally accepted
Xin chen Xu1,2,3
Qing fei Meng1,4Na Li1,2Zichuan Zou1,3Haonan Yang1,3Ang Li1,5Fusheng Ge1,2Jian Meng1,6*
Zi xue Ding1,2*- 1Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China
- 2Southeast University affiliated Xuzhou Central Hospital, Xuzhou, China
- 3Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China
- 4Southeast University affiliated Xuzhou Central Hospital, Xu Zhou, China
- 5Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou, China
- 6Southeast University affiliated Xuzhou Central Hospita, Xuzhou, China
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The mitochondrial genome of Erodium stephanianum (Geraniaceae) exhibits remarkable complexity revealed through nanopore sequencing, which has unveiled both chloroplast-tomitochondrion gene transfer and extensive DNA rearrangements. We collected leaf samples from E. stephanianum in June 2023, subsequently extracting total DNA and sequencing the mitogenome using both Oxford Nanopore and Illumina technologies. The assembly yielded a circular mitochondrial genome of 365,414 base pairs, encompassing 28 unique protein-coding genes, 18 tRNA genes, and 3 rRNA genes. Notably, 55 fragments, totaling 58,305 base pairs, showcased sequence homology between the chloroplast and mitochondrion, indicating substantial gene transfer with implications for evolutionary adaptation. Furthermore, codon usage analysis revealed preferential codon utilization, while microsatellite and repeat sequence analyses identified numerous SSRs and tandem repeats within the mitogenome. Phylogenetic analysis positioned E. stephanianum within the Geraniales order, closely clustering with Geranium maderense. This study highlights the dynamic evolution of mitochondrial genomes in E. stephanianum, emphasizing the significance of interorganellar gene transfer and genome rearrangement.
Keywords: Erodium stephanianum Willd., Mitochondrial genome (mitogenome), Repeated sequences (RS), phylogenetic relationship analysis, RNA Editing
Received: 05 Jun 2025; Accepted: 04 Jul 2025.
Copyright: © 2025 Xu, Meng, Li, Zou, Yang, Li, Ge, Meng and Ding. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jian Meng, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China
Zi xue Ding, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China
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