Feasibility in the Detection of Sentinel Lymph Node-Associated Blood Vessels Using Intravital Microscopy in Patients Undergoing Sentinel Lymph Node Biopsy for Melanoma

Emmanuel Gabriel^1*Daniel Fisher²Minhyung Kim²Kristopher Attwood²Valerie Francescutti²John Kane²Sharon Evans²Joseph J Skitzki²

• ¹Mayo Clinic in Florida, Jacksonville, United States
• ²Roswell Park Comprehensive Cancer Center, Buffalo, United States

While the clinical focus on the sentinel lymph node biopsy (SLNB) is the presence of intra-or extra-nodal metastases, preclinical studies suggest that tumor-draining SLNB-associated vascular architecture and adhesion properties are altered regardless of SLNB positivity. Human intravital microscopy (HIVM) has defined blood vessel abnormalities that may impact lymphocyte adhesion and systemic drug delivery at primary melanoma sites. In this pilot study of HIVM during melanoma SLNB, we sought to determine the feasibility of obtaining HIVM observations of SLNB-associated vessels. We successfully performed HIVM in all 20 SLNB patients, and 7 were found to have nodal micrometastases by standard pathology. HIVM was capable of identifying both functional and non-functional SLNB-associated vessels based on the presence or absence of fluorescent dye uptake, respectively. Comparing vessel characteristics as a secondary exploratory objective, no statistically significant differences were noted in the diameter, flow rate, functionality, or shear stress of SLNB-associated blood vessels between positive and negative SLNBs, which may likely have been a reflection of the minimal disease burden. Nonetheless, these initial observations provide the framework to optimize future trials of HIVM in cancer patients.