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ORIGINAL RESEARCH article

Front. Med., 18 September 2025

Sec. Pulmonary Medicine

Volume 12 - 2025 | https://doi.org/10.3389/fmed.2025.1592734

Predictive value of serum interleukin-33 and thymic stromal lymphopoietin for the risk of acute exacerbation in patients with chronic obstructive pulmonary disease

Shijia Yin&#x;Shijia Yin1Kang Xu&#x;Kang Xu1Shi WuShi Wu1Hua Liu
Hua Liu2*Zhen Ding
Zhen Ding1*
  • 1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Anhui Medical University, Hefei, China
  • 2Anhui Institutes for Food and Drug Control, Hefei, China

Background: Acute exacerbation in patients with chronic obstructive pulmonary disease (COPD) lacks reliable biomarkers. This study aimed to explore the predictive value of serum interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP) for acute exacerbations of COPD (AECOPD).

Methods: This retrospective study included patients with stable-phase COPD between June 2023 and June 2024 at the Third Affiliated Hospital of Anhui Medical University. Patients were assigned to the low-risk and high-risk groups according to the number and severity of AECOPD events. Multivariable logistic regression analysis was used to identify the factors of AECOPD risk. Receiver operating characteristic (ROC) curves were used to assess the predictive value of IL-33 and TSLP for AECOPD risk.

Results: A total of 76 patients were enrolled, with 33 in the low-risk group and 43 in the high-risk group. Serum IL-33 (835.30 vs. 770.30, p = 0.008) and TSLP (102.20 vs. 90.45, p < 0.001) levels were higher in the high-risk group than in the low-risk group. Serum TSLP (odds ratio (OR) = 1.095, 95% confidence interval (CI): 1.013–1.184, p = 0.022) was independently associated with AECOPD. The area under the curve (AUC) for IL-33 in predicting AE was 0.677 (95%CI: 0.554–0.801), with 93.0% sensitivity and 39.4% specificity. The AUC for TSLP was 0.768 (95%CI: 0.659–0.878), with 76.7% sensitivity and 72.7% specificity. Combined prediction using IL-33 and TSLP yielded an AUC of 0.779 (95%CI: 0.669–0.888), with 81.4% sensitivity and 72.7% specificity.

Conclusion: High serum TSLP might be associated with an increased risk of AECOPD. While IL-33 alone showed high sensitivity and low specificity, its potential predictive value may be worth exploring.

Introduction

Chronic obstructive pulmonary disease (COPD) is a common, heterogeneous pulmonary disease characterized by chronic respiratory symptoms due to airway and/or alveolar abnormalities, leading to persistent, usually progressive airflow limitation (1). In 2019, the global prevalence of COPD reached 212.3 million, with over 16 million new cases and more than 3.28 million deaths, making it the leading cause of mortality among chronic respiratory diseases (2). Acute exacerbations (AE) are significant events in the progression of COPD (1). These exacerbations result in a decline in lung function and quality of life and an increase in mortality (3). As the disease advances, both the frequency and severity of acute exacerbations of COPD (AECOPD) rise, contributing to a gradual decline in lung function and severely impacting patients’ quality of life and overall prognosis (1, 3). Treatment for AECOPD can alleviate symptoms but does not prevent lung function deterioration (3). Therefore, early prediction of AE risk and reducing its frequency are key to improving COPD prognosis (4).

Accurate assessment of the condition of COPD patients can help identify and prevent AEs, optimize the allocation of medical resources, reduce the economic burden on patients, and improve their quality of life (5). Available studies suggest that the history of AEs is the most reliable predictor of AECOPD risk (6, 7). However, this approach lacks a pathophysiological explanation of high and low exacerbation risk and does not provide directions for research on clinically targeted therapies (8). Interleukin (IL)-33 is encoded by the IL-33 gene and is a member of the IL-1 family. As an alarmin and a cytokine, IL-33 has a strong ability to drive type 2 inflammation, playing a key role in airway inflammation (9). Type 2 inflammation is involved in many cases of COPD (10). IL-33 mediates immune responses and alveolar damage, and its expression is upregulated in patients with COPD and is associated with disease progression and possibly AE risk (11). Thymic stromal lymphopoietin (TSLP) is a member of the IL-2 family and is released during respiratory epithelial cell damage. It plays a critical role in activating immune cell populations, such as mast cells, eosinophils, and type 2 innate lymphoid cells (12). Elevated TSLP levels have been detected in the serum of patients with COPD and are considered potentially associated with AE risk (12).

Nevertheless, the value of these two biomarkers in predicting AE risk in patients with COPD remains unclear. Therefore, this study aimed to explore the predictive value of serum IL-33 and TSLP for AECOPD.

Materials and methods

Study design and patients

This retrospective study included patients with stable-phase COPD treated between June 2023 and June 2024 at the Department of Respiratory and Critical Care Medicine of the Third Affiliated Hospital of Anhui Medical University. The study was approved by the Ethics Committee of the Third Affiliated Hospital of Anhui Medical University (2023–055-01). The requirement for individual informed consent was waived by the committee due to the retrospective nature of the study. The inclusion criteria were (1) diagnosis of COPD based on the Guidelines for Diagnosis and Treatment of Chronic Obstructive Pulmonary Disease (2021 Revised Edition) (13), (2) initially in the stable phase, and (3) clear consciousness and normal communication ability. The exclusion criteria were (1) incomplete clinical data, (2) comorbid asthma, tuberculosis, lung cancer, pulmonary fibrosis, or bronchiectasis, (3) severe dysfunction of the heart, liver, kidneys, or other organs, or (4) mental or cognitive impairments.

Patients were assigned to the low-risk and high-risk groups according to the number and severity of AE. Patients with ≤1 moderate to severe AE (not leading to hospital admission) within 1 year were assigned to the low-risk group, while patients with ≥2 moderate to severe AEs or ≥1 AE that led to hospitalization within 1 year were assigned to the high-risk group (1417).

Data collection and definition

Data were collected on sex, age, body mass index (BMI), and the number of hospitalizations due to AE. IL-33 and TSLP levels in the stable phase were determined routinely during the study period using ELISA kits (Guangzhou Huayun Biotechnology Co., Ltd., Guangzhou, China), according to the manufacturer’s instructions. During the study period, pulmonary function was assessed during the stable phase using a MasterScreen spirometer (Jaeger, Germany). The measurements included forced expiratory volume in 1 s (FEV1), FEV1 as a percentage of the predicted value (FEV1%pred), forced vital capacity (FVC), FVC as a percentage of the predicted value (FVC% pred), and the FEV1/FVC ratio. Each patient underwent three repeated tests, and the best result was recorded. The testing procedures adhered to the standards established by the American Thoracic Society (13). All measurements were performed in the context of a routine medical examination for COPD.

The diagnosis of AE was mainly based on the fact that one or more of the main symptoms, such as dyspnea, cough, and expectoration, worsen within a short period (usually several days to weeks), and the degree of change in symptoms exceeds the daily fluctuation range. There are also changes in the characteristics of sputum (such as an increase in sputum volume, purulent sputum, etc.) in COPD patients (18).

Statistical analysis

Data analysis was performed using SPSS 26.0 (IBM, Armonk, NY, USA). Continuous data with a normal distribution were expressed as means ± standard deviations and analyzed using the independent samples t-test. Non-normally distributed data were expressed as medians (interquartile ranges) and analyzed using the Mann–Whitney U-test. Categorical data were expressed as n (%) and analyzed using the chi-squared test. Univariable and multivariable logistic regression analyses were performed to identify factors influencing the risk of AECOPD. Variables with p < 0.10 in the univariable analyses were included in the multivariable models; the results were presented as odds ratio (OR) and 95% confidence interval (CI). Receiver operating characteristic (ROC) curves were used to evaluate the predictive value of IL-33 and TSLP for AE risk. Two-sided p-values <0.05 were considered statistically significant.

Results

Characteristics of the low-risk and high-risk groups

The patients were divided into the low-risk (n = 33) and high-risk (n = 43) groups based on the occurrence of AE (Figure 1). No statistically significant differences were observed between groups in age, sex, smoking history, family history, white blood cells (WBC), eosinophils (EOS), neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), or red cell distribution width (RDW) (p > 0.05). BMI was significantly higher in the low-risk group compared with the high-risk group (22.96 ± 3.51 vs. 21.09 ± 4.08 kg/m2, p = 0.039). Serum IL-33 (median, 770.30 vs. 835.30 pg./mL, p = 0.008) and TSLP (median, 90.45 vs. 102.20 pg./mL, p < 0.001) levels were significantly lower in the low-risk group compared with the high-risk group (Table 1).

Figure 1
Flowchart outlining a study of 160 COPD patients from September 2023 to March 2024. After applying exclusion criteria, 76 patients were included. These were divided into two groups: Non-AECOPD (33 patients) and AECOPD (43 patients). Both groups had no exclusions due to adverse events, and all patients were included in the final analysis.

Figure 1. Flowchart for screening research population.

Table 1
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Table 1. Characteristics of the low risk and high risk groups.

Logistic regression analysis of the factors associated with the risk of AECOPD

The univariable logistic regression analyses showed that BMI (OR = 0.879, 95%CI: 0.775–0.998, p = 0.039), FEV1/FVC (OR = 0.962, 95%CI: 0.924–1.002, p = 0.061), serum IL-33 levels (OR = 1.005, 95%CI: 1.001–1.010, p = 0.010), and serum TSLP levels (OR = 1.062, 95%CI: 1.021–1.105, p = 0.003) were associated with AECOPD. The variables with p < 0.10 in the univariable analyses were included in the multivariable logistic regression analysis. Serum TSLP (OR = 1.095, 95%CI: 1.013–1.184, p = 0.022) was identified as independently associated with AECOPD (Table 2).

Table 2
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Table 2. Logistic regression analysis of factors associated with the risk of AECOPD.

Predictive value of serum IL-33 and TSLP for the risk of AECOPD

At the optimal cutoff value of 712.95 pg./mL for IL-33, the AUC was 0.677 (95% CI: 0.554–0.801), sensitivity was 93.0%, and specificity was 39.4%. At the optimal cutoff value of 95.33 pg./mL for TSLP, the AUC was 0.768 (95% CI: 0.659–0.878), sensitivity was 76.7%, and specificity was 72.7%. The combined prediction using IL-33 and TSLP yielded an AUC of 0.779 (95% CI: 0.669–0.888), with a sensitivity of 81.4% and specificity of 72.7% (Figure 2 and Table 3).

Figure 2
ROC curve comparing TSLP, IL-33, and TSLP+IL-33. Sensitivity is on the y-axis, and 1-Specificity is on the x-axis. TSLP is shown as a dashed line, IL-33 as a dotted line, TSLP+IL-33 as a solid line, and the reference line is diagonal.

Figure 2. Receiver operating characteristics (ROC) curves showing the predictive value of serum interleukin (IL-33) and thymic stromal lymphopoietin (TSLP) for the risk of AECOPD.

Table 3
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Table 3. Predictive value of serum IL-33 and TSLP for the risk of AECOPD.

Discussion

The results suggested that high serum TSLP is associated with an increased risk of AECOPD. While IL-33 alone showed high sensitivity and low specificity, its potential predictive value may be worth exploring.

The study showed that the average serum IL-33 level in the high-risk group for AE was higher than in the low-risk group. The difference could be associated with the role of IL-33 in the pathogenesis of acute exacerbations in COPD. Indeed, IL-33 is a member of the IL-1 cytokine family and plays a crucial role in innate and adaptive immune responses (9). IL-33 is expressed in endothelial cells, epithelial cells, and fibroblast-like cells, making it present in multiple organs, and its functions in cancer and allergic inflammation are well-recognized (1921). The epithelial expression of IL-33 is upregulated in asthma and COPD and is associated with disease severity (22). Furthermore, IL-33 promotes inflammation by binding to the IL-33 receptor, growth stimulation expressed gene 2 (ST2), which is present in various cells, including neutrophils, eosinophils, macrophages, basophils, and mast cells. This interaction drives the production of type 2 and non-type 2 inflammatory cytokines, leading to airway smooth muscle contraction, goblet cell mucus production, and fibroblast activation (23), contributing to AEs in COPD. Consequently, the serum IL-33 levels in the high-risk group for AEs were higher than in the low-risk group. Furthermore, the AUC for IL-33 in predicting AE risk was 0.677 (95% CI: 0.554–0.801), with a sensitivity of 93.0% and a specificity of 39.4%. Although the predictive efficiency of serum IL-33 for AE risk is relatively low, its high sensitivity suggests that IL-33 could potentially become a key biomarker and therapeutic target for COPD, pending further confirmation through additional studies. Nevertheless, IL-33 has been identified as a potential therapeutic target for COPD (2426).

The present study also showed that the serum TSLP levels in the high-risk group for AEs were significantly higher than in the low-risk group. This difference may be related to the pathophysiological role of TSLP in AECOPD. TSLP is produced by epithelial cells in various tissues, such as the lungs, skin, and gastrointestinal tract, as well as by dendritic cells, keratinocytes, stromal cells, basophils, and mast cells (24, 27, 28). TSLP is classified as an “alarmin” and can be secreted by pulmonary structural and immune cells in response to respiratory viruses, air pollutants, allergens, or stimuli such as IL-4, IL-13, and tumor necrosis factor (TNF)-α (29). Elevated levels of TSLP mRNA and protein have been observed in the bronchial mucosa of patients with COPD (30). TSLP has broad immunomodulatory effects, influencing mast cells, type 1 helper T cells, and other cells that produce type 2 and non-type 2 inflammatory cytokines. This process mediates type 2 and non-type 2 inflammation, leading to airway smooth muscle contraction, goblet cell mucus production, and increased excitability of sensory neurons (23), contributing to AECOPD.

This study found that the AUC for TSLP in predicting AE risk was 0.768 (95% CI: 0.659–0.878), with a sensitivity of 76.7% and a specificity of 72.7%. A previous study showed that TSLP could be predictive of AE events but only in patients with low EOS counts. Such a subgroup analysis was not possible in the present study due to the sample size and the retrospective nature of the study. Combining IL-33 and TSLP yielded an AUC of 0.779 (95% CI: 0.669–0.888), with a sensitivity of 81.4% and a specificity of 72.7%, showing limited improvement compared with TSLP alone, suggesting limited added value. Moreover, this study identified serum TSLP as being independently associated with AE, suggesting that TSLP may serve as a novel biomarker for predicting AEs. TSLP (AUC = 0.768) appears to perform similarly to serum amyloid A (AUC = 0.789) and IL-6 (AUC = 0.762) for the prediction of AECOPD progression (31). Some models for predicting AEs in COPD are available [e.g., ACCEPT (31), ACCEPT 2.0 (32), and artificial intelligence (33)], and whether combining them with TSLP and IL-33 levels could improve their predictive value should be investigated. The early identification and timely interventions could reduce the incidence of AE, slow the decline in lung function, improve quality of life, and enhance prognosis. Furthermore, this provides more clinical evidence for targeted therapy in COPD. Nevertheless, additional investigation is necessary since a study showed that high TLSP levels were related to a lower risk of AE in patients with low EOS counts (33).

This study had limitations. It was an observational study with a small sample size. Due to the retrospective nature of the study, the study could only rely on the data available in the charts, and reliable data were missing for too many patients, which could not be used for analysis. Hospitalization in patients with COPD can be used as a surrogate of AE events, as suggested by the literature (1417), but it is not perfect since the patients can be hospitalized for other causes; nevertheless, in patients with COPD, AE will be the most common cause. While the findings have theoretical significance, larger, multicenter real-world studies are required to validate the roles of IL-33 and TSLP in AECOPD and to further explore their mechanisms. It would provide stronger evidence for the prevention and treatment of COPD.

In conclusion, high serum TSLP levels are associated with an increased risk of AECOPD. The predictive value of IL-33 for AE risk is relatively low, but its high sensitivity suggests potential value. Serum TSLP has a relatively high predictive value. TSLP could be a key biomarker and therapeutic target for AECOPD. Further in-depth, larger-scale, real-world studies are necessary to determine the role of TSLP in controlling AE risk, alleviating lung function decline, improving the quality of life, and prognosis for COPD patients.

Data availability statement

The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding authors.

Ethics statement

The studies involving humans were approved by the Ethics Committee of The First People's Hospital of Hefei (2023-055-01). The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation was not required from the participants or the participants' legal guardians/next of kin because the requirement for individual informed consent was waived by the committee due to the retrospective nature of the study.

Author contributions

SY: Writing – original draft, Conceptualization, Writing – review & editing, Formal analysis, Data curation. KX: Formal analysis, Writing – original draft, Data curation, Conceptualization. SW: Project administration, Methodology, Writing – original draft. HL: Software, Writing – review & editing, Resources. ZD: Writing – review & editing, Validation, Visualization.

Funding

The author(s) declare that financial support was received for the research and/or publication of this article. The study was supported by Hefei Science and Technology Bureau Medical and Health “Loanto-Grant” R&D Project (#J2020Y09).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Generative AI statement

The authors declare that no Gen AI was used in the creation of this manuscript.

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Publisher’s note

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Keywords: chronic obstructive pulmonary disease, acute exacerbation, interleukin-33, thymic stromal lymphopoietin, risk prediction

Citation: Yin S, Xu K, Wu S, Liu H and Ding Z (2025) Predictive value of serum interleukin-33 and thymic stromal lymphopoietin for the risk of acute exacerbation in patients with chronic obstructive pulmonary disease. Front. Med. 12:1592734. doi: 10.3389/fmed.2025.1592734

Received: 13 March 2025; Accepted: 02 September 2025;
Published: 18 September 2025.

Edited by:

Susan M. Bueno, Pontificia Universidad Católica de Chile, Chile

Reviewed by:

Tomoya Harada, Tottori University Hospital, Japan
Yunhuan Liu, Tongji University, China

Copyright © 2025 Yin, Xu, Wu, Liu and Ding. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Hua Liu, YWhtdWxoQDE2My5jb20=; Zhen Ding, aW1kemhAMTYzLmNvbQ==

These authors have contributed equally to this work

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.