ORIGINAL RESEARCH article
Front. Med.
Sec. Pulmonary Medicine
Volume 12 - 2025 | doi: 10.3389/fmed.2025.1600429
Assessment of extracellular vesicle microRNAs detection in predicting benign and malignant pulmonary ground glass nodules
Provisionally accepted
- 1Second Affiliated Hospital of Soochow University, Suzhou, China
- 2Suzhou Kowloon Hospital, Suzhou, Jiangsu Province, China
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Objective: To assess extracellular vesicle microRNAs detection in predicting malignant and benign pulmonary ground glass nodules (GGNs).Methods: A retrospective study was conducted on 96 patients with pulmonary GGNs, hospitalized in Suzhou Kowloon Hospital and The Second Affiliated Hospital of Soochow University, Shanghai Jiaotong University School of Medicine between April 2021 and March 2022. Based on pathological diagnosis, 67 were in the malignant group and 29 in the benign group. Additionally, 50 healthy individuals from the same hospitals were enrolled as controls. Plasma exosomal microRNAs (miRNAs) levels were measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in the three groups. The diagnostic value of candidate miRNAs was analyzed using receiver operating characteristic (ROC) curves.Results: Nodule size and proportion of pure GGNs were significantly higher in the malignant group than the benign group (P < 0.05). Expression of miR-17-5p and miR-1-3p was lower in malignant GGNs compared to benign ones, while miR-20b-5p, miR-9-5p, and miR-92a-2-5p were higher (P < 0.05). Compared to healthy controls, miR-1-3p and miR-17-5p were decreased, and miR-92a-2-5p, miR-9-5p, and miR-20b-5p were increased in malignant GGNs (P < 0.05). Among individual markers, miR-1-3p had the highest sensitivity and specificity. Sensitivity ranking: miR-1-3p > miR-20b-5p > miR-92a-2-5p > miR-9-5p > miR-17-5p; specificity ranking: miR-92a-2-5p > miR-17-5p > miR-1-3p > miR-9-5p > miR-20b-5p. Combining miR-1-3p and miR-92a-2-5p yielded an AUC of 1.000, with 100% sensitivity and specificity. In the healthy group comparison, miR-1-3p remained the most accurate single marker. Specificity ranking: miR-1-3p > miR-20b-5p > miR-92a-2-5p > miR-9-5p > miR-17-5p; sensitivity ranking: miR-1-3p > miR-17-5p > miR-92a-2-5p > miR-20b-5p > miR-9-5p.Conclusion: miR-1-3p, miR-9-5p, miR-17-5p, miR-20b-5p, and miR-92a-2-5p show promise as diagnostic biomarkers for GGN.
Keywords: Pulmonary ground glass nodules, Extracellular vesicle microRNA, diagnostic value, Sensitivity, liquid biopsy
Received: 26 Mar 2025; Accepted: 10 Jun 2025.
Copyright: © 2025 Ji, Xing, Zhong, Li, Cheng, Pan and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Minhua Shi, Second Affiliated Hospital of Soochow University, Suzhou, China
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