ORIGINAL RESEARCH article
Front. Med.
Sec. Hepatobiliary Diseases
Volume 12 - 2025 | doi: 10.3389/fmed.2025.1620367
This article is part of the Research TopicGut microbiome-driven Pathogenesis and Intervention Strategies in Liver DiseasesView all articles
Evaluation of the protective effect of paeoniflorin on alcoholic liver damage and its effect on intestinal flora
Provisionally accepted
Pengfei Du*
Changjiang Wang- Second Hospital of Jiaxing City, Jiaxing, China
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The study investigates the protective effect of paeoniflorin on alcoholic liver damage and its impact on intestinal flora. Alcoholic liver disease, exacerbated by excessive drinking, poses significant health risks, including liver damage and disruptions in intestinal flora. Despite paeoniflorin's known therapeutic benefits for various liver diseases, its effects on alcoholic liver disease and associated intestinal flora disturbances are underexplored. This research utilizes in vitro cell models and in vivo mouse models to evaluate paeoniflorin's hepatoprotective properties. The methods involved treating liver cells and mice with paeoniflorin under controlled experimental conditions. The in vitro experiments included treating liver cells with varying concentrations of paeoniflorin and measuring cell viability using CCK-8 assays. In vivo experiments involved administering paeoniflorin to alcohol-fed mice and analyzing blood biochemical indicators, liver histopathology, and intestinal flora composition through 16S rRNA gene sequencing. Key findings indicate that paeoniflorin significantly enhances liver cell survival, reduces alcohol-induced liver damage, and restores liver function markers to nearnormal levels. Additionally, paeoniflorin mitigates inflammatory responses and oxidative stress in liver cells. The analysis of intestinal flora revealed that paeoniflorin treatment restores the balance of beneficial bacteria disrupted by alcohol, particularly increasing Lactobacillus levels and decreasing harmful bacteria such as Clostridiales.The study uniquely demonstrates paeoniflorin's dual role in protecting against alcoholic liver damage and regulating intestinal flora, highlighting its potential as a therapeutic agent for alcoholic liver disease.
Keywords: paeoniflorin, alcoholic liver damage, Jian Gan San, intestinal flora, Network Pharmacology
Received: 29 Apr 2025; Accepted: 20 Jun 2025.
Copyright: © 2025 Du and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Pengfei Du, Second Hospital of Jiaxing City, Jiaxing, China
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