ORIGINAL RESEARCH article
Front. Med.
Sec. Translational Medicine
Volume 12 - 2025 | doi: 10.3389/fmed.2025.1661339
Influence of Different Urine Preservation Methods and Addition of Preservatives on Quantitative Detection of 24-hour Urinary Protein
Provisionally accepted
- Affiliated Maternity and Child Health Care Hospital of Nantong University, Nantong, China
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Objective: This study aimed to elucidate the effect of adding xylene as a preservative on 24-hour urine protein quantification under different storage temperaturesThis study aimed to elucidate the effect of the presence or absence of xylene on the 24-hour urine protein quantification test. Methods: From January 2020 to August 2020, our hospital selected a total of 80 outpatients with kidney diseases. Under different storage temperature conditions, urine samples containing or not containing xylene were collected simultaneously. Then, one-way analysis of variance was used to study the effects of preservatives and temperature on the 24-hour urine protein quantification test. Receiver operating characteristic (ROC) analysis was used to examine the effect of adding preservatives on the accuracy of the 24-hour urinary protein concentration determination. Results: Based on the results of the control group, there was no statistically significant difference in the 24-hour urine protein concentration between the preservative group and the group without preservatives at 37 ℃, 24-26 ℃ or 4 ℃ (F = 0.006, P = 0.993; F = 0.013, P = 0.987; F = 0.022, P = 0.977P > 0.05). The results of the ROC analysis indicated excellent diagnostic accuracy for proteinuria detection across all storage conditions (AUC: 0.992-0.994). The accuracy of urine samples stored without preservatives was comparable to, and in some cases (e.g., at 4°C) exhibited perfect specificity (100%) alongside high sensitivity (97.4%), matching the direct detection in the control group. The results of the receiver operating characteristic analysis indicated that the accuracy of detecting proteinuria in urine samples without preservatives was comparable to that of the direct detection of proteinuria in the control group. Conclusion: Storing 24-hour urine protein specimens at room temperature without using preservatives is a safe, simple, and feasible methodDirectly preserving 24-hour urine protein specimensat room temperature without using preservatives is a safe, simple, and feasible method. This method is suitable for It is crucial for quantitative detection of 24-hour urine protein and is recommended for wide application in clinical practice.
Keywords: 24-Hour urinary protein, preservation, Xylene, Quantitative detection, Sample stability
Received: 07 Jul 2025; Accepted: 29 Sep 2025.
Copyright: © 2025 Bao, Sun, Geng and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiaohua Yuan, dahuastudent@sohu.com
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