ORIGINAL RESEARCH article
Front. Med.
Sec. Obstetrics and Gynecology
This article is part of the Research TopicAdvances in New Biomarkers for the Diagnosis and Therapy of Gynaecological TumoursView all 11 articles
ZNF582 hypermethylation as a highly specific biomarker for triage of 1 high-grade cervical lesions in HR-HPV positive women
Provisionally accepted
- Department of Statistical Office, The Affiliated Changsha Central Hospital, Hengyang Medical School , University of South China, Hunan, China
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Objective Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary cause of cervical cancer (CC). DNA methylation is a promising biomarker for cervical cancer screening. This study aimed to validate the clinical efficacy of a cytological DNA methylation-based ZNF 582 methylation (ZNF582m) test for triage in detecting cervical intraepithelial neoplasia (CIN) grade 3 or higher (CIN3+) among women with HR-HPV infection.
Methods This case-control study examined 199 women who tested positive for HR-HPV from the colposcopy clinic. The cohort included 15 cases of CC, 37 cases of CIN3, 45 cases of CIN2, 49 cases of CIN1, and 53 normal cases. Using cervical pathology results as a reference, the performance of the ZNF582m test for detecting CIN3+ was evaluated and compared with liquid-based cytology (LBC) and P16/Ki67 double stain cytology (DSC).
Results: ZNF582m test showed statistically significant differences in pathological results between CIN2 and CIN3 (P < 0.001). The area under the receiver operating characteristic curve (AUC) for distinguishing between
Keywords: ZNF582 methylation, p16/Ki67, cervical screening, Cervical Intraepithelial Neoplasia, High-risk human papillomavirus
Received: 18 Aug 2025; Accepted: 30 Nov 2025.
Copyright: © 2025 Lian, Jiang, Jiang, Wang and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Haijun Luo
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