Yu Geng1†
Zhen Jia- 1Interventional Department, Binhai County People’s Hospital, Yancheng, Jiangsu, China
- 2Department of Acupuncture and Massage, The First People’s Hospital of Xuzhou, Xuzhou, Jiangsu, China
- 3Neonatal Medical Center, Children’s Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
- 4Department of Gynaecology and Obstetrics, Binhai County People’s Hospital, Yancheng, Jiangsu, China
- 5Department of Laboratory, Haidong Second People’s Hospital, Haidong, Qinghai, China
Metabolic dysfunction-associated steatotic liver disease (MASLD), previously referred to as non-alcoholic fatty liver disease (NAFLD), has become the most common chronic liver disease globally, with its incidence rising annually. MASLD is closely linked to metabolic syndrome and can progress from simple steatosis to more severe stages, including non-alcoholic steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma (HCC), affecting 13%–38.2% of cases. Notably, in 40%–50% of patients, this progression occurs without cirrhosis. The dysregulation of glucose and lipid metabolism is a fundamental pathological mechanism in MASLD and its transition to HCC. Key factors include insulin resistance, increased gluconeogenesis, impaired β-oxidation, oxidative stress, and chronic inflammation, all of which contribute to a tumor-promoting hepatic microenvironment. This review provides a comprehensive analysis of the latest research on MASLD-related HCC, emphasizing disturbances in glucose metabolism (such as disrupted hepatic insulin signaling, key enzymes like G6Pase and PK, and miRNAs such as miR-22-3p that induce Warburg effects), lipid imbalances (for example, upregulation of FASN/ACC and downregulation of PPARα targets like CPT1A), and the crosstalk between various pathways (including mTORC1, AMPK/ACC, FXR, and NF-κB/JNK). It also explores metabolic regulators such as DKK3, FGF21, and O-GlcNAcylation, and examines the role of the gut microbiota in modulating short-chain fatty acids, bile acids, and NLRP3 inflammasome activation in disease progression. By integrating the latest advancements in basic and clinical research, this article presents a solid theoretical framework for early diagnosis, risk assessment, biomarker development, and precision therapies. It also highlights promising therapeutic targets, including PPARα agonists, mTOR inhibitors, FGF21 analogs, and microbiota interventions, while proposing future directions in multi-omics and personalized treatment strategies.
1 Introduction
Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty liver disease (NAFLD), represents a paradigm shift in nomenclature, as endorsed by the 2023 AASLD/EASL consensus (1). Unlike traditional NAFLD, which emphasized the exclusion of alcohol as an etiology, MASLD positively defines the condition by the presence of hepatic steatosis (≥ 5%) coupled with at least one cardiometabolic risk factor (e.g., obesity, type 2 diabetes, dyslipidemia), underscoring metabolic dysfunction as the primary driver (2). Pathophysiologically, this redefinition highlights insulin resistance and lipotoxicity as central mechanisms, distinguishing MASLD from broader NAFLD spectra that included non-metabolic idiopathic steatosis. MASLD is now recognized as a major contributor to cirrhosis and hepatocellular carcinoma (HCC). The progression of this condition unfolds in several distinct stages, beginning with simple hepatic steatosis (NAFL), which can advance to non-alcoholic steatohepatitis (NASH) and may subsequently progress to liver fibrosis, cirrhosis, or eventually HCC (3). The Global Burden of Disease study has identified MASLD as a significant and rapidly growing risk factor for liver cancer, with notably higher incidence rates observed in younger individuals and males (4). In Western countries, MASLD has become the leading risk factor for hepatocellular carcinoma (5).
The primary mechanisms underlying MASLD are closely linked to the dysregulation of glucose and lipid metabolism, encompassing insulin resistance, lipid accumulation, inflammatory responses, oxidative stress, and other related pathological processes (6, 7). Research indicates that excessive consumption of high-fat and high-sugar diets is a major factor contributing to the development of MASLD. These dietary patterns promote abnormal fat accumulation in the liver, which in turn triggers the onset of chronic liver disease (8, 9). Moreover, the progression of MASLD is multifaceted, involving the interplay of various pathways, including metabolic dysregulation, liver inflammation, cell apoptosis, and fibrosis (10, 11). The pathological features of MASLD-related HCC are notably complex, presenting clinical and molecular characteristics that differ from those of conventional cirrhotic liver cancer. In patients with MASLD, around 40%–50% of HCC cases develop in the absence of apparent cirrhosis (12). This phenomenon presents considerable challenges to traditional HCC screening methods, which rely on the presence of liver cirrhosis. Research into the underlying mechanisms of MASLD-related HCC has revealed that these processes are closely linked to prolonged metabolic stress, including insulin resistance, hyperglycemia, lipotoxicity, oxidative stress, and chronic inflammation. Together, these factors create a hepatic microenvironment conducive to the development of liver cancer.
Currently, effective treatment options for MASLD and its associated liver cancer are limited. Traditional approaches primarily focus on lifestyle modifications, including weight loss, dietary improvements, and increased physical activity, to help reduce the risk of disease development (13, 14). For patients already diagnosed with MASLD, regular monitoring and early screening are essential. Moreover, for individuals with NASH or those at high risk, personalized treatment strategies—such as pharmacological interventions to address insulin resistance or non-invasive therapies to reduce lipotoxicity—are critical. A thorough understanding of the regulatory mechanisms governing glucose and lipid metabolism is therefore key to developing new therapeutic approaches. Researchers are increasingly investigating potential therapeutic targets, including AMP-activated protein kinase (AMPK), liver X receptor (LXR), and farnesoid X receptor (FXR), all of which play pivotal roles in regulating glucose and lipid metabolism as well as inflammatory responses (15, 16). For instance, the activation of AMPK is believed to reduce hepatic lipid accumulation and insulin resistance, thereby helping to slow the progression of MASLD (17). Additionally, modulating hepatic metabolic signaling pathways, such as enhancing metabolic parameters with sodium-glucose cotransporter type 2 (SGLT2) inhibitors, offers a promising new approach for the treatment of MASLD (18).
In conclusion, as our understanding of the connection between MASLD and HCC advances, the medical community is actively seeking more effective treatment strategies to prevent liver cancer and slow disease progression. Future research will focus on elucidating the causal relationships between these conditions, developing novel biomarkers and diagnostic tools, and creating more targeted treatment approaches. Additionally, raising public health awareness and implementing supportive public health policies will be crucial to addressing this issue. Only through the integrated application of multidisciplinary knowledge and techniques can we more effectively tackle this global health challenge. This paper will focus on the role of glucose and lipid metabolism in MASLD-related liver cancer, systematically reviewing the latest research to provide a theoretical foundation for understanding MASLD and its progression, with the goal of offering valuable insights for future clinical treatment strategies.
2 Dysregulation of glucose and lipid metabolism in MASLD-related hepatocellular carcinoma
2.1 Central role of glucose and lipid metabolism
The disruption of glucose and lipid metabolism is a key driver in the development of MASLD and its progression to HCC. This disruption encompasses several processes, including insulin resistance, increased gluconeogenesis, elevated fatty acid synthesis, impaired β-oxidation, oxidative stress, and inflammatory responses. These metabolic abnormalities are interconnected and work together to promote hepatic fat accumulation, inflammation, fibrosis, and the formation of a carcinogenic microenvironment, ultimately facilitating the progression from MASLD to NASH and HCC.
Abnormal glucose metabolism in MASLD is mainly characterized by insulin resistance, increased gluconeogenesis, and impaired glucose utilization. Patients with MASLD typically display marked insulin resistance, which disrupts hepatic glucose metabolism and triggers systemic metabolic disturbances. This, in turn, exacerbates hepatic fat accumulation and inflammation (19, 20). Increased gluconeogenesis leads to continuous glucose release from the liver, worsening hyperglycemia and, in turn, promoting fat accumulation, thereby establishing a vicious cycle. Additionally, disruptions in vitamin A metabolism also impact glucose metabolism. Vitamin A modulates glucose metabolism through retinoic acid-mediated transcriptional networks (21). In MASLD, the PNPLA3-I148M variant is linked to reduced serum retinol levels and elevated hepatic retinyl esters, indicating that an imbalance in vitamin A metabolism may contribute to disease progression.
Abnormal lipid metabolism in MASLD is characterized by increased hepatic fatty acid synthesis, impaired β-oxidation, and lipid accumulation. Under normal conditions, the liver converts fatty acids into energy through β-oxidation. However, in MASLD, this process is inhibited, resulting in fatty acid buildup and the development of hepatocellular steatosis (22). As MASLD progresses to NASH and liver fibrosis, fatty acid synthesis intensifies, further stressing the liver and promoting inflammation and fibrosis. These processes create a conducive environment for the development of liver cancer (23). In a diet-induced MASLD mouse model, increased hepatic triglyceride and cholesterol levels, along with heightened insulin resistance and inflammation, were closely linked to the development of HCC (24).
Certain bioactive molecules play essential regulatory roles in glucose and lipid metabolism, as well as in liver cancer development. For example, Dickkopf-3 (DKK3) is significantly downregulated in the livers of MASLD patients and obese mice. Hepatocyte-specific overexpression of DKK3 improves insulin sensitivity, glucose tolerance, lipid metabolism, and suppresses inflammation, while DKK3 deficiency exacerbates pathological changes (25). Mechanistically, DKK3 inhibits the activation of the P38/JNK pathway by binding to apoptosis signal-regulating kinase 1 (ASK1) (26).
Disruption of glucose and lipid metabolism is a key factor in the pathogenesis of MASLD and its associated liver cancer. Through mechanisms such as insulin resistance, fat accumulation, oxidative stress, and inflammation, these metabolic abnormalities collectively contribute to liver damage and the creation of a carcinogenic microenvironment. Imbalances in vitamin A metabolism and alterations in the gut microbiome further aggravate this process. Interventions aimed at regulating glucose and lipid metabolism, including targeting key molecules like DKK3, may offer promising new therapeutic strategies for MASLD and related liver cancer.
2.2 The link between glucose and lipid metabolism dysregulation and hepatocellular carcinoma development
Abnormal glucose and lipid metabolism plays a critical role in the development of HCC, primarily by promoting cell proliferation, inhibiting apoptosis, and inducing genetic mutations. MASLD is recognized as a significant risk factor for HCC, with studies showing that 13%–38.2% of MASLD patients will develop HCC, especially in cases where the disease progresses to NASH (27). As MASLD progresses, dysregulation of lipid metabolism results in abnormal fat accumulation in the liver, which triggers inflammatory responses and apoptosis in cells, thereby promoting the proliferation and survival of cancer cells. Specifically, metabolic reprogramming allows tumor cells to thrive in hypoxic and nutrient-poor environments, using fatty acids as an energy source to fuel their rapid proliferation and growth (28, 29).
Dysregulation of glucose and lipid metabolism induces oxidative stress and inflammatory responses, which are critical in the progression from MASLD to liver cancer. In the livers of MASLD patients, oxidative stress markers are elevated, correlating with lipid peroxidation and cellular damage (30, 31). Oxidative stress not only damages hepatocytes but also accelerates liver tissue fibrosis. Chronic inflammation, driven by fat accumulation, exacerbates hepatocyte injury and contributes to the formation of a tumor microenvironment, acting as a catalyst for both fibrosis and HCC (32). Additionally, alterations in the gut microbiota, through the modulation of short-chain fatty acid production, impact liver inflammation and immune responses, thereby contributing to the development of liver cancer.
Furthermore, studies have shown that lipid metabolism disorders associated with MASLD are linked to the occurrence of genetic mutations. For instance, the activation of transcription factors such as E2F1 and E2F2 is closely associated with HCC development. These factors regulate fatty acid oxidation and synthesis, which in turn affect the metabolic state of hepatocytes, promoting the progression of HCC (6). Abnormal glucose and lipid metabolism not only impacts hepatocyte proliferation and apoptosis but also, by altering the liver’s microenvironment, promotes fibrosis, ultimately contributing to the development of HCC (33).
In terms of metabolic reprogramming, tumor cells increase lipid synthesis and suppress fatty acid oxidation to acquire more energy and biosynthetic materials. This metabolic shift enables tumor cells to establish a microenvironment within the liver that supports their growth, thereby further accelerating the onset and progression of liver cancer (34). Understanding the role of glucose and lipid metabolism in HCC development is crucial, as it not only enhances our comprehension of the pathogenesis of liver cancer but also identifies potential targets for new therapeutic strategies. By intervening in these metabolic pathways, novel approaches for the prevention and treatment of liver cancer may be uncovered.
3 The Role of glucose metabolism in MASLD-related hepatocellular carcinoma
3.1 Abnormal hepatic insulin signaling pathway
In MASLD and its associated HCC, abnormalities in the hepatic insulin signaling pathway play a crucial role in the dysregulation of glucose metabolism. Disruption of this pathway not only increases gluconeogenesis but also impairs glucose utilization (20, 35). Under normal physiological conditions, insulin activates its receptor to promote glucose uptake and storage in the liver, stimulates glycogen synthesis, and inhibits gluconeogenesis. However, in MASLD patients, the onset of insulin resistance diminishes the liver’s ability to respond to insulin, resulting in enhanced gluconeogenesis and elevated blood glucose levels (36).
Abnormalities in the insulin signaling pathway also disrupt lipid metabolism, promoting lipid synthesis and creating a harmful metabolic cycle. Studies have shown that in the state of insulin resistance, the liver continues to promote lipid synthesis while failing to adequately suppress gluconeogenesis, resulting in abnormal elevations in both blood glucose and blood lipids (37, 38). This vicious cycle not only worsens MASLD but may also accelerate its progression to more severe stages, such as NASH and liver cancer.
In-depth research into the hepatic insulin signaling pathway has revealed several molecular pathways involved in this process. One such pathway involves AMPK, a key metabolic regulator that plays a critical role in insulin signaling. Activation of AMPK enhances insulin sensitivity, inhibits hepatic lipid synthesis, and promotes fatty acid oxidation, thereby helping to mitigate the progression of MASLD (39). Additionally, vascular endothelial growth factor B (VEGFB) has been shown to improve insulin resistance and lipid metabolism by modulating the PI3K/AKT pathway (40).
Abnormalities in the hepatic insulin signaling pathway not only disrupt glucose and lipid metabolism but are also closely linked to chronic low-grade inflammation, oxidative stress, and other mechanisms that drive the onset and progression of MASLD. As a result, targeting the insulin signaling pathway could represent a key therapeutic strategy for MASLD and its associated liver cancer. Enhancing the function of this pathway may help mitigate the progression of MASLD and reduce the risk of liver cancer development.
3.2 Regulation of key enzymes and transporters
In the study of MASLD-related HCC, the regulation of key enzymes and transporters is crucial for maintaining the balance of glucose and lipid metabolism. Alterations in the activity of key enzymes, such as hepatic glucose-6-phosphatase and pyruvate kinase, can directly impact hepatic glucose metabolism. For instance, glucose-6-phosphatase (G6Pase) plays a vital role in converting glucose-6-phosphate to glucose in the liver, a process central to gluconeogenesis. Research indicates that in MASLD, the expression of G6Pase may be upregulated, leading to increased hepatic gluconeogenesis and exacerbating hepatic fat accumulation (41, 42). In contrast, pyruvate kinase (PK), a key enzyme in glycolysis, when less active, drives a shift in metabolism toward fat synthesis, playing a crucial role in the pathological progression of MASLD. Furthermore, studies have shown that inhibiting PK activity can reduce hepatic fat accumulation, highlighting its potential therapeutic value in MASLD and liver cancer (43).
On the other hand, intestinal gluconeogenesis (IGN) plays a significant role in regulating hepatic glucose metabolism. IGN refers to the glucose produced in the intestines following the absorption of specific nutrients, facilitated by an intestinal-brain-liver neural signaling mechanism. Studies have shown that IGN can effectively prevent hepatic steatosis and mitigate the onset of MASLD by reducing hepatic fat synthesis and lipid influx. Specifically, transgenic mice with enhanced IGN exhibit a marked reduction in hepatic fat accumulation when fed high-calorie diets, a phenomenon closely linked to decreased hepatic inflammation and fibrosis (41, 44). Furthermore, the upregulation of IGN is closely linked to the balance between hepatic gluconeogenesis and lipogenesis, offering new potential targets for the treatment of MASLD.
In conclusion, key enzymes (such as G6Pase and PK) and transporters are central to the regulation of glucose and lipid metabolism in MASLD, while intestinal gluconeogenesis presents a novel approach for regulating hepatic glucose metabolism. These findings offer a theoretical foundation for developing MASLD treatment strategies. Future research could focus on therapeutic interventions targeting these key enzymes and transporters to restore metabolic balance, thereby slowing the progression of MASLD.
3.3 Mechanisms of miRNA regulation of glucose metabolism
In the liver, miR-22-3p is a key regulator of glucose metabolism and plays a crucial role in the development of MASLD and liver cancer. Research has shown that miR-22-3p is among the most abundant microRNAs (miRNAs) in the liver, with alterations in its expression closely linked to hepatic steatosis, insulin resistance, and cancer progression (45). Downregulation of miR-22-3p is frequently observed in liver cancer, suggesting its potential involvement in the pathophysiology of liver-related metabolic disorders. However, the precise role and mechanisms of miR-22-3p in the context of obesity and MASLD (metabolic-associated steatotic liver disease) remain unclear, warranting further investigation to elucidate its effects. In miR-22 knockout (miR-22KO) mice subjected to a high-fat diet to induce obesity, no significant symptoms were observed under normal conditions. However, under the high-fat diet challenge, miR-22KO mice exhibited marked glucose intolerance, fat accumulation, hepatomegaly, and hepatic steatosis. Further analysis revealed an upregulation of glycolytic and lipid uptake enzymes in the liver, highlighting the critical role of miR-22 in regulating hepatic metabolism.
Furthermore, the expression of miR-22-3p in Huh7 liver cancer cells was found to be inversely related to its expression in non-tumor tissues, displaying a distinct “Warburg effect,” characterized by increased glycolysis and suppressed mitochondrial respiration (46, 47). Specifically, this “Warburg effect” is achieved because miR-22-3p directly targets and suppresses the expression of key enzymes involved in the mitochondrial tricarboxylic acid (TCA) cycle and oxidative phosphorylation. This metabolic shift toward aerobic glycolysis, even in the presence of oxygen, provides rapidly proliferating cancer cells with not only ATP but also essential biosynthetic precursors for nucleotide and lipid synthesis, thereby fueling tumor growth and progression. This observation suggests that miR-22-3p may exert distinct regulatory effects under different physiological and pathological conditions, thereby influencing both hepatic metabolism and tumor development. These findings highlight the potential of miR-22-3p as a therapeutic target, especially in the treatment of obesity-associated MASLD and liver cancer, where altering its expression or function could offer novel therapeutic approaches for patients.
In conclusion, miR-22-3p plays a key role in regulating hepatic glucose metabolism, and its loss may contribute to the progression of obesity and MASLD, ultimately influencing the development of liver cancer. A deeper investigation into this mechanism will improve our understanding of the pathological processes underlying MASLD and hepatocellular carcinoma, offering new targets and strategies for therapeutic intervention. It is important to note that the regulatory functions of miRNAs such as miR-22-3p are not isolated; rather, they are integral components of the broader metabolic signaling network. They often serve as critical nodes that interact with other major pathways discussed in this reviewte to the progI3K/AKT and AMPK signaling cascades They often serve as critical nodes that interact wit(19). Understanding this interplay is essential for developing targeted therapies capable of restoring metabolic homeostasis.
4 Lipid metabolism abnormalities and their role in MASLD-related hepatocellular carcinoma
4.1 Imbalance between fatty acid synthesis and oxidation
The imbalance between fatty acid synthesis and oxidation is a critical factor in the development of MASLD and its progression to associated HCC. This condition is often characterized by altered expression of key enzymes, including fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACC). The upregulation of these enzymes enhances fatty acid synthesis in the liver, leading to excessive fatty acid accumulation. This accumulation can disrupt hepatocyte function and trigger inflammation, thereby accelerating the pathological progression of the disease (27, 48). Studies have demonstrated that in MASLD patients, the expression levels of FASN and ACC are markedly elevated, suggesting that the activation of the fatty acid synthesis pathway is closely linked to the pathophysiological changes associated with MASLD (49, 50). Moreover, the imbalance in fatty acid synthesis is closely linked to insulin resistance, inflammation, and liver fibrosis, all of which collectively contribute to the progression toward HCC (19).
At the same time, impaired β-oxidation of fatty acids plays a crucial role in the progression of MASLD. Under normal conditions, fatty acids undergo β-oxidation in the mitochondria, generating energy for cellular functions. However, in MASLD patients, this process is frequently disrupted, resulting in the accumulation of fatty acids within hepatocytes. This accumulation further exacerbates hepatocyte damage and promotes apoptosis (28, 51). For instance, transcription factors like E2F1 and E2F2 have been implicated in the metabolic reprogramming of fatty acids in MASLD-related HCC. Their elevated expression is inversely associated with the downregulation of enzymes involved in fatty acid β-oxidation, such as carnitine palmitoyltransferase 2 (CPT2), highlighting their significant role in fatty acid metabolism (22). This metabolic reprogramming not only worsens the fatty accumulation in the liver but also facilitates the malignant transformation of hepatocytes. In addition, lipotoxicity arises from the imbalance in fatty acid synthesis/oxidation, where excess saturated free fatty acids induce hepatocyte damage via multiple interconnected pathways. Overloaded mitochondria generate ROS, triggering ER stress and unfolded protein response (UPR), which activates JNK-mediated apoptosis (52). Concurrently, diacylglycerol accumulation inhibits insulin signaling through PKC translocation, exacerbating hyperglycemia and ceramide synthesis; ceramides, in turn, activate NLRP3 inflammasome, amplifying inflammation and fibrosis. These lipotoxic cascades foster a protumorigenic microenvironment, promoting HCC via genomic instability and immune evasion. Targeting lipotoxicity (e.g., via SCD1 inhibition) thus offers dual benefits in halting MASLD progression and HCC oncogenesis.
The imbalance between fatty acid synthesis and oxidation represents not only a key pathological change in the progression of MASLD but also serves as a biological foundation for its progression to HCC. Researchers are increasingly focused on developing interventions that target these metabolic pathways, aiming to offer new therapeutic strategies for MASLD and its associated complications. By gaining a deeper understanding of fatty acid metabolism in MASLD development, more effective preventive and therapeutic measures can be devised, ultimately improving patient outcomes and reducing the incidence of HCC.
4.2 Regulatory role of PPARα and its target genes in lipid metabolism
Peroxisome proliferator-activated receptor alpha (PPARα) is a key regulator of lipid metabolism, primarily influencing fatty acid oxidation and inflammatory responses. It is highly expressed in the liver, where it plays a crucial role in the uptake, esterification, and transport of fatty acids, as well as regulating the expression of genes involved in lipoprotein metabolism (53). Studies have demonstrated that PPARα enhances fatty acid β-oxidation by activating target genes, such as carnitine palmitoyltransferase 1A (CPT1A) and peroxisomal acyl-CoA oxidase 1 (ACOX1), thereby helping to maintain lipid homeostasis (54, 55). Additionally, PPARα plays a role in inhibiting hepatic inflammation, reducing liver fibrosis, and preventing hepatic steatosis, thereby mitigating the progression of MASLD and NASH (56). The role of PPARα in MASLD and NASH is particularly significant. Its agonists, such as fenofibrate, have been shown to notably improve lipid metabolism and reduce hepatic inflammation (57).
Clinical trials have highlighted the potential of PPARα agonists in reducing lipid levels and improving liver function, particularly in the treatment of MASLD and NASH (58, 59). However, the expression and activity of PPARα decrease as MASLD and NASH progress, likely due to impaired fatty acid metabolism and heightened hepatic inflammation (60). The use of PPARα agonists in treating MASLD and NASH has spurred extensive research. Several PPARα agonists are currently undergoing clinical trials, demonstrating effectiveness in reducing liver fat accumulation and improving liver function. For instance, some novel PPARα agonists notably enhance the expression of genes related to fatty acid oxidation in the liver, reduce hepatic inflammation, and improve the metabolic status of patients (61). These studies provide a crucial theoretical basis for considering PPARα as a potential therapeutic target for MASLD and NASH. Future research may further clarify its role in other metabolic disorders. Recent clinical data further support the applicability of PPARα agonists. For example, lanifibranor, a pan-PPAR agonist, has advanced to Phase 3 trials (as of 2025), showing significant reductions in liver fat (up to 45%), fibrosis improvement, and enhanced insulin sensitivity in MASH patients (62). However, challenges include mild gastrointestinal side effects and the need for long-term monitoring of efficacy in HCC prevention. Real-world evidence from post-marketing studies of fenofibrate indicates sustained lipid-lowering effects but variable fibrosis resolution, emphasizing the importance of combination therapies for optimal outcomes (63).
In conclusion, PPARα plays a key regulatory role in lipid metabolism, and its agonists hold significant therapeutic potential for MASLD and NASH. Future research is anticipated to offer new insights and evidence to support the development of more effective treatment strategies.
4.3 Crosstalk between glucose and lipid metabolism and the critical signaling pathway
4.3.1 mTOR signaling pathway
mTORC1 (mechanistic target of rapamycin complex 1), a specific complex of mTOR, plays a pivotal role in regulating glucose and lipid metabolism. It governs critical processes such as fatty acid synthesis, glycolysis, and amino acid metabolism. By modulating energy balance through pathways like the AMPK signaling pathway, mTORC1 influences hepatocyte metabolism. In MASLD and liver cancer, the activation of mTORC1 is linked to increased lipid accumulation and inflammation. Elevated fatty acid levels activate mTORC1, which in turn promotes lipid synthesis and glucose metabolism, driving the progression of MASLD (64). In liver cancer, mTORC1 activation promotes cell proliferation, inhibits apoptosis, and triggers metabolic reprogramming, thereby supporting tumorigenesis (65). mTORC1 regulates key downstream effectors, such as S6K1 and 4E-BP1, which promote hepatocyte growth and support tumor cell survival (66).
Excessive activation of mTORC1 profoundly alters cellular metabolism, driving hepatocyte proliferation and facilitating the development of MASLD-related liver cancer. In MASLD, elevated fatty acid levels activate mTORC1, enhancing lipid synthesis and cell proliferation while suppressing apoptosis, thereby accelerating the progression of liver cancer (67). mTORC1 activation also upregulates genes such as sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN), thereby promoting lipid accumulation and hepatocyte growth (68). argeting mTORC1 with inhibitors such as rapamycin has demonstrated potential in reducing liver cancer cell proliferation and survival, particularly in models of MASLD-associated liver cancer (69). This suggests that inhibiting mTORC1 could provide innovative therapeutic approaches for treating both MASLD and liver cancer.
4.3.2 AMPK/ACC signaling pathway
The AMPK/ACC pathway has garnered significant attention in studies of HCC associated with MASLD. As a key regulator of cellular energy balance, AMPK plays a critical role in liver cancer development by influencing lipid metabolism, glycolysis, and the cell cycle (70). Activation of AMPK inhibits acetyl-CoA carboxylase (ACC), thereby reducing fatty acid synthesis and promoting fatty acid oxidation, which offers protective effects in MASLD (71). Increased expression of sebum protein P (SeP) in MASLD promotes lipid accumulation via the AMPK/ACC pathway. Inhibiting SeP can reduce triglyceride accumulation, presenting a potential novel target for diagnosis and treatment (72). Additionally, 5-aminobutyric acid (5-ALA) activates AMPK, highlighting its essential role in regulating lipid metabolism in a high-fat diet-induced mouse model (73).
Dysregulated lipid metabolism is a key factor in the development of liver cancer. FASN has been shown to promote colorectal cancer cell proliferation and metastasis through the AMPK/mTOR pathway, a mechanism that may also be relevant to liver cancer (74). Furthermore, CD147 reprograms fatty acid metabolism in liver cancer cells through the Akt/mTOR/SREBP1c and P38/PPARα pathways, further underscoring the critical role of lipid metabolism in liver cancer (75). In conclusion, the AMPK/ACC pathway plays a crucial role in regulating lipid metabolism and provides new insights into the diagnosis and treatment of MASLD-related liver cancer. Further investigation of this pathway and its molecular mechanisms could pave the way for more effective strategies in preventing and treating MASLD and associated liver cancers.
4.3.3 FXR signaling pathway
Farnesoid X receptor (FXR), a nuclear receptor that primarily regulates bile acid metabolism and plays a key role in lipid and glucose metabolism, is essential in the pathophysiology of MASLD-related liver cancer. Dysregulation of FXR in both MASLD and HCC may contribute to disease progression and worsening (76). FXR agonists, such as Obeticholic Acid (OCA), have demonstrated therapeutic potential in treating NASH by improving fibrosis and potentially delaying the progression to cirrhosis. However, side effects, including alterations in atherosclerotic lipid profiles, may necessitate adjunctive therapy with statins (77, 78).
The role of FXR in the gut-liver axis has been extensively studied, with intestinal microbiota dysbiosis identified as a factor in the progression of both MASLD and HCC. FXR regulates bile acid metabolism and maintains gut microbiota balance, influencing intestinal permeability and immune activation, which may, in turn, affect MASLD progression (79). Combined regulation of FXR with other targets, such as soluble epoxide hydrolase (sEH), has shown enhanced therapeutic effects in NASH, particularly in terms of anti-inflammatory and anti-fibrotic outcomes (80). In conclusion, the regulatory mechanisms of FXR and its involvement in the gut-liver axis offer significant clinical and research potential for the prevention and treatment of MASLD and HCC, particularly through multi-target therapeutic approaches.
4.3.4 NF-κB/JNK signaling pathway
The NF-κB pathway plays a pivotal role in liver cancer progression, with its activation associated with an unfavorable prognosis and stem cell-like traits (81). Dysregulated NF-κB activation is linked to a range of inflammatory diseases and cancers, positioning it as a promising target for therapeutic intervention (82). In MASLD-associated HCC, NF-κB activation drives tumor progression and is closely linked to hepatic fibrosis. Natural compounds that modulate this pathway have demonstrated potential in reducing fibrosis and in the development of novel anti-fibrotic therapies (83). Furthermore, the non-classical NF-κB pathway plays a critical role in the regulation of liver diseases, with small molecule inhibitors emerging as promising candidates for managing liver injury (84).
The JNK pathway is also essential in HCC, impacting cell death, proliferation, and carcinogenesis. Studies indicate a cross-regulation between the JNK and NF-κB pathways, which may affect tumor progression by modulating cellular survival and apoptosis (85). JNK activation is associated with oxidative stress and mitochondrial dysfunction, driving hepatocyte apoptosis in cancer cells (86). In the treatment of MASLD-related liver cancer, targeting the NF-κB and JNK pathways may provide therapeutic benefits. Certain natural products and compounds have shown potential in inhibiting liver cancer cell proliferation and migration by suppressing these pathways (87, 88). Moreover, microRNAs like miR-26b enhance chemotherapy sensitivity by inhibiting NF-κB, offering promising new therapeutic strategies (89). In conclusion, the JNK and NF-κB pathways are crucial in MASLD-related liver cancer, representing promising targets for the development of novel diagnostic and therapeutic approaches.
5 Metabolic regulatory factors and their role in MASLD-Related hepatocellular carcinoma
5.1 Fibroblast growth factor 21
Fibroblast growth factor 21 (FGF21) is a hormone-like protein predominantly produced in the liver and adipose tissue, playing a key role in regulating glucose and lipid metabolism. Recent studies have shown that FGF21 enhances insulin sensitivity and reduces hepatic fat accumulation, offering protective effects against MASLD and its progression to HCC (90). FGF21 regulates lipid metabolism by inhibiting lipogenesis and enhancing insulin sensitivity in the liver, thereby improving its lipid profile (91). During MASLD progression, FGF21 expression levels are often linked to disease severity. A deficiency in FGF21 worsens conditions that promote liver tumorigenesis, thereby increasing the risk of HCC (92).
Clinical research has established a connection between genetic variations in FGF21 and patients’ dietary patterns as well as addictive behaviors, including smoking and alcohol consumption. This highlights the gene’s significant role in metabolic disorders (90). Moreover, FGF21 analogs, including Pegbelfermin, are being explored as potential treatments for MASLD and NASH. These compounds have shown promise in enhancing liver metabolic health, with clinical trials demonstrating favorable safety and efficacy profiles (93). The role of FGF21 as a biomarker in MASLD and HCC is gaining increasing recognition. Elevated serum levels of FGF21 are now regarded as a key indicator of MASLD, reflecting metabolic disturbances that occur throughout the disease progression (94). Studies have also shown that the upregulation of FGF21 is linked to the progression of various cancers, including breast cancer. This suggests that FGF21 may contribute to tumor growth within the tumor microenvironment (95). Thus, FGF21 not only holds promise as a potential biomarker for MASLD but may also serve as a therapeutic target for cancers like HCC. Clinical applicability of FGF21 analogs has been demonstrated in recent Phase 2 trials (2023-2025). Pegozafermin reduced liver fat by 27%–42% and improved fibrosis in MASH patients, with good tolerability but potential injection-site reactions (96). Efruxifermin showed efficacy in compensated cirrhosis due to MASH, resolving steatohepatitis in 41% of cases, though long-term HCC prevention data are limited (97). Challenges include variable patient responses and the need for combination with other agents for enhanced efficacy.
While research on FGF21 continues to evolve, current findings offer solid theoretical support for its potential use in treating MASLD and HCC. Future studies should prioritize the clinical application of FGF21 and its analogs, investigating their mechanisms across various stages of MASLD and HCC to inform the development of more effective therapeutic strategies.
5.2 O-GlcNAc modification and its metabolic regulatory functions
O-GlcNAc modification is a crucial post-translational modification that regulates cellular metabolic processes by detecting changes in nutrient levels through UDP-GlcNAc, which is derived from the hexosamine biosynthesis pathway (HBP). This modification is integral to the development of various diseases, particularly metabolic disorders such as diabetes, cancer, and neurodegenerative diseases, where its dysregulation is recognized as a key driver of disease progression (98, 99). In response to acute stress, O-GlcNAc modification is rapidly upregulated, boosting cellular stress resistance and promoting survival (100). Studies have demonstrated that O-GlcNAcylation regulates enzymes involved in lipid metabolism, such as FASN, thereby supporting tumor growth and survival (101, 102).
In MASLD, dysregulated O-GlcNAcylation is linked to hepatic lipid accumulation, insulin resistance, and the progression of liver cancer (103, 104). O-GlcNAcylation also modulates insulin signaling, impacting insulin secretion and β-cell function (105). O-GlcNAc transferase (OGT), the key enzyme regulating this modification, plays a pivotal role in the progression of MASLD, particularly in modulating mitochondrial function and oxidative stress (106). Inhibiting OGT has demonstrated potential in enhancing mitochondrial function, reducing lipid accumulation, and alleviating inflammation, positioning OGT as a promising therapeutic target for MASLD (103). In conclusion, O-GlcNAc modification, regulated by OGT, plays a crucial role in metabolic diseases like MASLD. Targeting OGT may provide innovative therapeutic approaches for these conditions.
6 Gut microbiota and glucose-lipid metabolism interactions inmasld-related hepatocellular carcinoma
6.1 Impact of gut microbiota dysbiosis on liver metabolism
Alterations in the composition of the gut microbiota are closely associated with liver glucose and lipid metabolism, as well as inflammatory responses. Recent studies have underscored the crucial role of the gut microbiota in the pathogenesis of MASLD, particularly in the regulation of hepatic lipid metabolism and inflammation. Dysbiosis, or microbial imbalance, triggers inflammation, which is recognized as a key factor in the progression of MASLD. When the intestinal barrier is compromised, harmful substances such as bacterial endotoxins can enter the bloodstream, leading to hepatic inflammation and metabolic disturbances (107). Studies indicates that the gut microbiota, through its metabolic byproducts such as short-chain fatty acids and bile acids, plays a key role in regulating hepatic lipid metabolism and immune responses. This, in turn, influences hepatocyte function, impacting lipid accumulation and inflammatory processes (108).
Alterations in specific gut microbiota, such as an elevated abundance of Desulfovibrio, have been associated with the progression of MASLD. Desulfovibrio is a bacterium known for metabolizing sulfur compounds, and its metabolic byproducts may modulate liver lipid metabolism and inflammatory processes, thereby contributing to the development of MASLD (109). As a prominent sulfate-reducing bacterium, an overgrowth of Desulfovibrio leads to excessive production of hydrogen sulfide (H2S). At high concentrations, H2S can exert cytotoxic effects, impairing the integrity of the intestinal epithelial barrier and increasing its permeability. This ‘leaky gut’ phenomenon facilitates the translocation of bacterial endotoxins into the portal circulation, directly fueling hepatic inflammation. Furthermore, dysbiosis is closely linked to other components of metabolic syndrome, including insulin resistance, all of which contribute to abnormal hepatic fat accumulation and hepatocyte damage (110). In the management of MASLD, modulation of the gut microbiota has garnered significant interest. Strategies such as probiotics, prebiotics, and fecal microbiota transplantation are recognized as effective in enhancing gut microbiota balance and mitigating liver inflammation and fat accumulation (111). These studies offer valuable insights into the prevention and treatment of MASLD, highlighting the essential role of the gut microbiota in liver metabolism.
In conclusion, dysbiosis of the gut microbiota affects liver metabolic function and plays a critical role in the progression of MASLD by modulating inflammatory responses. Future research should focus on uncovering the underlying mechanisms of gut microbiota regulation and its potential therapeutic applications in preventing and treating MASLD, providing new strategies for clinical management.
6.2 Gut-liver axis mechanisms and regulation of metabolites
The gut-liver axis describes the bidirectional communication between the gut and liver, where metabolites produced by the gut microbiota regulate liver metabolism and immune function. Recent studies highlight the significant roles of metabolites, including short-chain fatty acids (SCFAs) and bile acids, in this process. SCFAs, produced through the fermentation of dietary fibers by gut microbiota, not only supply energy to intestinal cells but also impact liver health by modulating lipid metabolism and inflammatory responses (112). SCFAs can activate GLP-1 (glucagon-like peptide-1) receptors, which stimulates insulin secretion and enhances insulin sensitivity, thereby helping to reduce the risk of hepatic fat accumulation (113). Similarly, bile acids, as key signaling molecules between the liver and gut, regulate lipid metabolism by influencing hepatic metabolic functions through feedback mechanisms (114). Dysregulation of bile acids is strongly linked to the development of MASLD, with research indicating that impaired bile acid metabolism in the liver may worsen fatty liver disease.
Additionally, the integrity of the gut barrier is essential for the proper functioning of the gut-liver axis. When the intestinal barrier is compromised, endotoxins like gut-derived lipopolysaccharides (LPS) can enter the bloodstream, triggering chronic inflammation in the liver and accelerating the progression of fatty liver disease (115). Studies have demonstrated that when the gut barrier is compromised, bacterial components such as LPS can reach the liver, where they activate immune cells and trigger excessive inflammatory responses (116, 117). Specifically, this activation is mediated primarily through the recognition of LPS by Toll-like receptor 4 (TLR4) expressed on hepatic macrophages (Kupffer cells). This binding initiates a downstream signaling cascade that potently activates the NF-κB and JNK pathways, leading to the transcription and release of pro-inflammatory cytokines like TNF-α and IL-6. This chronic, low-grade inflammation is a key driver of hepatocyte injury, insulin resistance, and the progression from simple steatosis to NASH and fibrosis, potentially progressing to liver cancer over time.
Regulating the mechanisms of the gut-liver axis and its metabolites presents promising new avenues for treating MASLD and its associated liver cancer. Modifying the microbial community, strengthening gut barrier integrity, and utilizing metabolites such as SCFAs and bile acids may reduce liver inflammation, improve metabolic function, and contribute significantly to preventing and managing MASLD and its progression. These findings provide a crucial theoretical framework and practical direction for future clinical research and drug development.
6.3 The potential of gut microbiota intervention in improving metabolic disorders
Intervening with the gut microbiota holds considerable promise for the prevention and treatment of MASLD and its associated HCC. Recent studies have highlighted the close connection between changes in the gut microbiota and metabolic disorders, demonstrating their significant role in the onset and progression of MASLD. Interventions such as probiotics, dietary polysaccharides, and specific medications (e.g., Semaglutide) can effectively regulate the gut microbiota, thereby improving the metabolic status in MASLD. For instance, probiotics can enhance gut barrier function and modulate immune responses, potentially reducing liver inflammation and steatosis (118). Additionally, the consumption of dietary polysaccharides supports the growth of beneficial gut microbiota, leading to the production of SCFAs, which enhance insulin sensitivity and regulate hepatic lipid metabolism (119).
Semaglutide, a GLP-1 receptor agonist, has been investigated for its potential to improve the metabolic status of MASLD patients. It exerts its effects by regulating appetite, enhancing insulin secretion, and inhibiting hepatic glucose production, while also modulating the gut microbiota composition, promoting a more favorable metabolic profile (120). Studies have shown that Semaglutide treatment leads to a significant increase in gut microbiota diversity, with a notable rise in beneficial bacterial populations, further highlighting its potential for metabolic regulation. Modulating the gut microbiota not only helps improve metabolic disorders in MASLD but also prevents its progression to liver cancer. The progression of MASLD is closely associated with gut dysbiosis, inflammation, and alterations in the hepatic microenvironment. Through metabolites such as SCFAs and bile acids, the gut microbiota influences hepatic lipid metabolism and inflammation, playing a crucial role in maintaining liver health (121). Therefore, interventions targeting the gut microbiota, including probiotics, dietary polysaccharides, and pharmacological treatments, may provide novel strategies for the prevention and treatment of MASLD-related liver cancer. Moreover, studies have demonstrated that the interaction between the gut microbiota and inflammatory pathways, such as the NLRP3 inflammasome, plays a crucial role in MASLD and its progression. Modulating the gut microbiota may help reduce intestinal permeability and suppress inflammation, thereby alleviating liver damage and lowering the risk of liver cancer (122). Future research should focus on how gut microbiota interventions can enhance the metabolic status of MASLD patients and reduce the risk of liver cancer. Successfully implementing these interventions could offer valuable insights for the clinical management of MASLD and play a pivotal role in preventing and treating liver cancer.
To clarify the complex interactions between dysregulation of glucose and lipid metabolism, inflammatory pathways, and gut microbiota in the progression of MASLD-HCC, Table 1 offers a detailed summary of the key mechanisms and their roles in disease pathogenesis
Table 1. Key mechanisms of glucose and lipid metabolism dysregulation in non-alcoholic fatty liver disease-hepatocellular carcinoma (NAFLD-HCC).
7 Clinical interventions and future prospects for glucose and lipid metabolism in masld-related hepatocellular carcinoma
7.1 Current drugs and progress in clinical trials
Metabolic dysfunction associated steatotic liver disease is the most prevalent chronic liver disease worldwide and is strongly linked to metabolic syndrome, making it a significant public health concern. While no specific drugs have been approved for MASLD treatment, advancements have been made in developing therapies that target its underlying pathological mechanisms. PPARα agonists, which regulate lipid and glucose metabolism, have shown promising results in clinical trials by significantly reducing liver fat content and improving liver function (123). GLP-1 receptor agonists, such as Semaglutide, effectively reduce liver fat accumulation and metabolic disturbances by enhancing insulin sensitivity and promoting weight loss (124). FGF21 analogs regulate energy and lipid metabolism, helping to reduce liver fat and improve insulin resistance. Clinical trials have shown good tolerability and safety, though the long-term efficacy is still being evaluated (125). In addition, inhibitors targeting the NLRP3 inflammasome help reduce liver inflammation and fibrosis, offering a promising new approach for MASLD treatment.
Recent updates bolster these agents’ profiles: Resmetirom (THR-β agonist) achieved FDA approval in 2024, with MAESTRO-NASH Phase 3 data confirming 26% NASH resolution and fibrosis reduction, though limitations include pruritus (15% incidence) and understudied HCC endpoints (126). Semaglutide’s ESSENCE phase 3 trial (72-week interim, AASLD 2024) showed 62.9% MASH resolution without fibrosis worsening (vs. 34.1% placebo; EDP 28.9%, P < 0.0001) and 37.0% fibrosis improvement without MASH progression (vs. 22.5%; EDP 14.5%, P < 0.0001), via GLP-1 metabolic/inflammatory effects and 10.5% weight loss, with 30%–40% liver enzyme/fibrosis marker reductions; GI AEs increased (nausea 36%, diarrhea 27%), and modest fibrosis gains highlight combo therapy potential (127). Lanifibranor’s NATIVE phase 2b trial (24-week data, 2024) yielded broad CMH improvements in MASH patients via pan-PPAR agonism, including 26% TG reduction (AMD −0.5 mmol/L), 0.5% HbA1c lowering, hs-CRP drops (−2.2 mg/L), and DBP decreases (−3.9 mmHg), with adiponectin surges (> 4-fold in 80%) correlating to histological MASH resolution (49%) and fibrosis regression (34%), independent of average 2.5 kg weight gain (128).
Given the limited efficacy of single-target drugs, multi-target combination therapies have emerged as a key area of research. For example, combining GLP-1 receptor agonists with SGLT2 inhibitors yields a synergistic effect, enhancing metabolic indicators and promoting liver health (129). This combined approach aims to improve treatment outcomes by addressing multiple pathological mechanisms, including metabolic regulation and inflammation. As our understanding of the underlying mechanisms of MASLD deepens, drug development targeting multiple pathways will be crucial for future therapies, providing more comprehensive treatment options for patients.
Although preclinical studies have shown promising results, translating these findings into clinical benefits remains a challenge. For instance, the SCD1 inhibitor Aramchol is currently being assessed in Phase III clinical trials for NASH and liver fibrosis, including the ARMOR study (130). As of mid-2025, however, no Phase III clinical trial results have been published for MASLD-related HCC patient subgroups, underscoring the critical need for focused clinical research in this specific population (131, 132). In the future, the development of novel drugs is expected to advance into clinical trials, offering new hope for the treatment of MASLD and its associated complications, such as HCC.
7.2 Emerging therapeutic targets and technologies
Gene editing and miRNA interventions have emerged as promising approaches for treating MASLD and HCC. Technologies such as CRISPR-Cas9 enable precise modification of genes linked to MASLD and HCC, facilitating the identification of pathogenic genes and the development of personalized treatments. Targeting key genes, such as AKT1, has demonstrated potential in improving lipid metabolism and inhibiting tumor growth in MASLD-related liver cancer (133). Additionally, miRNAs are crucial in regulating lipid metabolism, inflammation, and fibrosis. Targeting specific miRNAs may provide new therapeutic avenues for treating MASLD and HCC (134). O-GlcNAc modification, which impacts metabolic pathways, is also being investigated for its role in liver metabolic disorders and its potential contribution to the development of MASLD (135). Furthermore, mTORC1 inhibitors are being investigated for their potential to improve lipid metabolism and reduce liver inflammation in MASLD. Inhibiting mTORC1 has shown promise in preventing the progression of MASLD to HCC by mitigating lipid accumulation and inflammation (136). However, challenges such as potential side effects and the complexity of the mTORC1 pathway persist. Further research is required to develop more targeted treatments that modulate mTORC1, ensuring a balance between efficacy and safety in clinical settings.
In conclusion, the integration of gene editing, miRNA intervention, O-GlcNAc modification regulation, and mTORC1 inhibition presents promising new strategies for treating MASLD and HCC. Ongoing research will refine these approaches, opening up new possibilities for clinical management.
7.3 Future research directions
Understanding the roles of these networks in the progression of MASLD and HCC requires a comprehensive analysis using advanced technologies such as metabolomics, transcriptomics, and single-cell RNA sequencing. These approaches can provide insights into how glucose-lipid metabolism influences hepatocyte biology and tumorigenesis (137). Personalized treatment strategies will be essential moving forward, as MASLD and liver cancer patients display considerable heterogeneity in metabolic profiles, genetics, and lifestyle. Integrating genomics and epigenetics can help identify genetic susceptibilities linked to MASLD, allowing for personalized prevention and treatment plans tailored to high-risk individuals (138). Additionally, designing interventions tailored to metabolic states—such as diet, exercise, and pharmacotherapy—will open up new avenues for managing MASLD. Integrating multi-omics is crucial for uncovering the metabolic heterogeneity of MASLD and its progression to liver cancer. Combining data from genomics, transcriptomics, metabolomics, and proteomics can help identify novel biomarkers for precision diagnosis and treatment. Additionally, exploring interactions between hepatocytes and their microenvironment, including immune and stellate cells, will shed light on their roles in the progression of MASLD and HCC (139).
To advance the field, future research should address key questions and prioritize targeted proposals. These include: (1) Biomarker-Guided Therapy: Can baseline serum FGF21 levels or circulating miR-22-3p expression profiles predict patient responses to metabolic therapies, enabling personalized treatment? This could involve clinical trials evaluating FGF21 as a biomarker for MASLD severity, guiding analog dosing to prevent HCC in high-risk patients, and using miR-22-3p for stratifying those with metabolic reprogramming to enable targeted miRNA therapies in glycolytic-dominant HCC. (2) Non-invasive Risk Stratification: How can multi-omics data—integrating genomics, metabolomics, and gut microbiome signatures—develop robust algorithms to stratify MASLD patients by HCC risk, optimizing surveillance? Specific queries, such as whether FGF21/miR-22-3p panels improve early HCC detection in MASLD cohorts, provide a roadmap for validation. (3) Causal Mechanisms of the Gut-Liver Axis: What are the roles of microbial-derived metabolites (e.g., bile acids or SCFAs) in hepatocyte transformation, and can interventions like engineered probiotics or postbiotics modify these pathways to prevent HCC? Finally, as the interactions between gut microbiota and host metabolism receive increasing attention, Table 2 summarizes emerging therapeutic targets and interventions for MASLD-HCC, emphasizing their potential to modulate glucose and lipid metabolism and reduce inflammation.
Table 2. Emerging therapeutic targets and interventions for glucose and lipid metabolism in non-alcoholic fatty liver disease-hepatocellular carcinoma (NAFLD-HCC).
8 Conclusion
In conclusion, studies on MASLD-related liver cancer is advancing rapidly, revealing the complex interactions between glucose-lipid metabolism disturbances, key regulatory factors, and the gut microbiota. These insights are crucial not only for deepening our understanding of the disease’s pathophysiology but also for developing novel therapeutic approaches. Figure 1 presents a succinct visual overview of these mechanisms, showing how metabolic disruptions initiate MASLD, how core pathway interactions intensify inflammation and lipid accumulation, and how the tumor microenvironment contributes to HCC development. The pivotal role of metabolic dysregulation in driving liver fat accumulation, hepatocellular carcinoma formation, and disease progression emphasizes the importance of comprehensive strategies for treatment and prevention. Targeting key molecules such as PPARα, FGF21, miRNAs, and O-GlcNAc modification holds promise for therapeutic interventions, positioning metabolic regulation as a central strategy for innovative treatments.
Figure 1. Mechanistic pathway of metabolic dysfunction-associated steatotic liver disease (MASLD) progression to hepatocellular carcinoma (HCC). This diagram illustrates the metabolic and molecular mechanisms driving the progression from MASLD to HCC. The liver is depicted centrally, with surrounding quadrants representing key processes: (1) Metabolic Input (left) highlights dietary and physiological inputs; (2) Core Pathway Interactions (upper right) shows signaling pathways; and (3) Tumor Microenvironment (lower right) depicts the tumor-supportive environment. Key elements include: high carbohydrate intake and cholesterol promoting SREBP-1c/ChREBP and VLDL secretion, respectively, exacerbated by insulin resistance and hepatic gluconeogenesis leading to MASLD; AMP-activatedproteinkinase (AMPK) as an upstream regulator phosphorylating and inhibiting ACC; farnesoid X receptor (FXR) and PPARα regulating lipid metabolism via SHP and CPT1-mediated β-oxidation, while mTORC1 activates S6K1/4E-BP1 and SREBP-1c, enhancing lipid droplet formation and inflammation through JNK-NF-κB; and the Warburg effect driving glycolysis and lactate accumulation, fostering HCC cell growth, fibrosis, and an immunosuppressive microenvironment with M2 macrophages and suppressed CD8+ T cells. Gut microbiota dysbiosis alters the pool of short-chainfattyacids (SCFAs) and bile acids, which activates JNK-NF-κB to amplify inflammation and/or fibrosis. Red arrow lines indicate promotion or enhancement (e.g., activation of pathways or processes), while blue arrow lines denote inhibition (e.g., suppression of immune responses or pathways).
Furthermore, the impact of the gut microbiota on liver metabolism and disease progression represents a promising area of research, indicating that modulating the microbiome could serve as a valuable complement to conventional therapies. Gaining a deeper understanding of the bidirectional interaction between the liver and the gut microbiome will be crucial for developing more effective intervention strategies. Additionally, the emergence of precision medicine, fueled by the integration of genomic, metabolic, and microbiome data, will facilitate the tailoring of treatments to individual patient profiles, ultimately improving therapeutic outcomes.
Despite significant advancements, challenges persist in translating these findings into clinical practice, especially regarding early diagnosis and personalized treatment. Current limitations include therapeutic safety concerns, such as pruritus and adverse lipid changes with FXR agonists like obeticholic acid, and uncertainties in long-term efficacy for emerging drugs like FGF21 analogs, which show short-term fibrosis reductions but require extended trials to confirm HCC prevention. Gaps also exist in addressing patient heterogeneity and comorbidities, which may reduce treatment responses and complicate real-world application. Future research should prioritize exploring the complexities of glucose-lipid metabolic networks, their interactions with the gut microbiota, and their roles in the progression of MASLD and liver cancer. By leveraging multi-omics technologies and deepening our understanding of underlying mechanisms, it will be possible to identify novel biomarkers for early detection, predict disease progression, and develop more effective, tailored treatment strategies. Ultimately, integrating these insights offers great potential for improving patient outcomes and quality of life, providing a comprehensive approach to managing MASLD-related liver cancer.
Author contributions
YG: Investigation, Methodology, Software, Visualization, Writing – original draft. LL: Methodology, Resources, Writing – original draft. YS: Resources, Visualization, Writing – original draft. LG: Validation, Writing – original draft. YW: Supervision, Writing – review & editing. ZJ: Conceptualization, Funding acquisition, Supervision, Writing – review & editing.
Funding
The author(s) declare financial support was received for the research and/or publication of this article. This work was supported by Qinghai Provincial Health and Wellness Commission Project (2024-wjzdx-135).
Acknowledgments
We thank the Figdraw platform for providing graphics-related help.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: MASLD, HCC, metabolic dysregulation, gut microbiota, therapeutic targets
Citation: Geng Y, Liu L, Sun Y, Guo L, Wu Y and Jia Z (2025) Metabolic dysregulation in MASLD-associated HCC: diagnostic biomarkers and therapeutic opportunities. Front. Med. 12:1705723. doi: 10.3389/fmed.2025.1705723
Received: 15 September 2025; Accepted: 21 October 2025;
Published: 04 November 2025.
Edited by:
Zhengyang Bao, Wuxi Maternity and Child Health Care Hospital, ChinaReviewed by:
Li-Da Wu, Nanjing Medical University, ChinaSiyuan Cheng, The First Affiliated Hospital of Jinan University, China
Copyright © 2025 Geng, Liu, Sun, Guo, Wu and Jia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zhen Jia, anlrMzEzODcxMDk3QDE2My5jb20=; Yuanjing Wu, MzEzODcxMDk3QHFxLmNvbQ==
†These authors have contributed equally to this work