Methyl jasmonate and salicylic acid synergism enhances phloroglucinol content in shoot cultures of Astragalus fruticosus Forssk

Mohamed El-Shazly^1*Rawia Abdel Hadi Zayed²Ashraf El-Sayed³Ahmed Elissawy¹Wafaa M Ismaeil²Shaimaa Fayez¹

• ¹Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Beni Suef, Egypt
• ²Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Al Sharqia, Egypt
• ³Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig, Al Sharqia, Egypt

Multiple shoot induction was obtained fromperformed on the explants of the stems and leaves of Astragalus fruticosus Forssk. stem and leaves explants after being cultured on Murashige and Skoog's (MS) medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) after over 8 weeks of culture. Elicitation, as a yield enhancement strategy, was developed to study the increment of phloroglucinol production using multiple in the cultured shoots to treat the cultures treated with methyl jasmonate (MJ) and salicylic acid (SA) at. Various concentrations (of 50 and 100 µg/ml) of MJ and SA and and a combination of both (at a final concentration of 50 µg/ml) over a period of 1-3 weeks, were used for the aim of enhancing the production of phloroglucinol for the highest biomass production. Methyl jasmonate (MJ), at a concentration of 100 µg/ml, exhibited showed a 5.06-fold increase in phloroglucinol content after three weeks comparing compared with the unelicited control. This is the first report on the elicitation of Astragalus fruticosus multiple stems and leaves shoot cultures using with the signal molecules, MJ and SA. The current findings might could be helpful for the in vitro manipulation and enhanced production of phloroglucinol.