Emerging Roles of the TRIM E3 Ubiquitin Ligases MID1 and MID2 in Cytokinesis

Abstract

Ubiquitination is a post-translational modification that consists of ubiquitin attachment to target proteins through sequential steps catalysed by activating (E1), conjugating (E2), and ligase (E3) enzymes. Protein ubiquitination is crucial for the regulation of many cellular processes not only by promoting proteasomal degradation of substrates but also re-localisation of cellular factors and modulation of protein activity. Great importance in orchestrating ubiquitination relies on E3 ligases as these proteins recognise the substrate that needs to be modified at the right time and place. Here we focus on two members of the TRIpartite Motif (TRIM) family of RING E3 ligases, MID1, and MID2. We discuss the recent findings on these developmental disease-related proteins analysing the link between their activity on essential factors and the regulation of cytokinesis highlighting the possible consequence of alteration of this process in pathological conditions.

Introduction

Cytokinesis and Ubiquitination

Cytokinesis is the final step of cell division that consists in the physical separation into two cells after nuclear and cytoplasmic content portioning. It requires coordinated actions of the cytoskeleton, membrane systems, and cell cycle engine, which are precisely controlled in space and time. Cytokinesis starts after anaphase and consists of different steps: central spindle assembly, division plane specification, contractile ring assembly, cytokinetic furrow ingression, midbody appearance, and abscission (Echard et al., 2004; Eggert et al., 2006). Following abscission, the residual midbody is either released in the extracellular medium, degraded by selective autophagy or persists in the cytoplasm of one daughter cell (Agromayor and Martin-Serrano, 2013). Interestingly, inherited midbodies can affect cell polarity, cell communication, stemness (Bernabe-Rubio et al., 2016; Antanaviciute et al., 2018; Li et al., 2018). Cytokinesis failure leads to defective mitosis and high chromosomal instability. Thus, for proper organisms growth and development a correct cell division is essential (D’Avino et al., 2015).

The activity of cytokinesis factors must be precisely orchestrated and oscillates by regulated post-translational modifications such as ubiquitination. Covalent conjugation of ubiquitin to a substrate is operated through sequential action of activating (E1), conjugating (E2), and ligase (E3) enzymes. Importantly, the E3 ubiquitin ligases recognise the specific substrates to be ubiquitinated (Komander and Rape, 2012). Ubiquitination is long known for driving cell cycle transitions. For example, metaphase-to-anaphase transition is triggered by the E3 ligase APC/C^CDC20 that promotes the degradation of cyclin B and securin, allowing mitotic exit (Teixeira and Reed, 2013). Ubiquitination is a signal not only for protein degradation but also for non-proteolytic fate through the building of chains with different ubiquitin linkages and topologies (Kulathu and Komander, 2012). As example, the E3 CRL3^KLH21 mono-ubiquitinates Aurora B allowing its MKLP2-mediated translocation to promote correct kinetochore–microtubule attachments during metaphase (Krupina et al., 2016). Further, a giant protein possessing E2/E3 activity, BRUCE, interacts with midbody components affecting the distribution of ubiquitin at the midbody site, in that being fundamental for abscission (Pohl and Jentsch, 2009).

Many other E3 ligases have been described to dynamically control cell cycle events through both proteolytic and non-proteolytic signals (Gilberto and Peter, 2017). The TRIpartite Motif (TRIM) family is the major sub-class of RING-E3 ubiquitin ligases counting over 70 members implicated in several physiological and pathological processes (Reymond et al., 2001; Meroni and Diez-Roux, 2005; Short and Cox, 2006; Hatakeyama, 2017). Here, we will focus on the role of two members of this family, TRIM18/Midline1/MID1 and TRIM1/Midline2/MID2 (from here onward MID1 and MID2), in cytokinesis.

MID1 and MID2 E3 Ubiquitin Ligases

Among the TRIM family, MID1, and MID2 are very close paralogues originating from a common ancestor after the invertebrate/vertebrate separation and predating the bony vertebrates appearance (Sardiello et al., 2008). Consistently, human MID1 and MID2 genes have a conserved genomic structure, are both located on the X chromosome, and share a high degree of identity (70%) at nucleotide level (Quaderi et al., 1997; Buchner et al., 1999). This similarity is patent also in their domain structure. MID1 and MID2 present the N-terminal hallmark of the TRIM family, the tripartite motif, composed of the catalytic RING domain followed by tandem B-Box 1 and B-box 2 and a Coiled-coil region. The TRIM family is further subdivided into 9 classes (C-I to C-IX) according to the domains present C-terminal to the tripartite module with the SPRY-containing C-IV subfamily being the most numerous (Reymond et al., 2001; Short and Cox, 2006). MID1 and MID2 C-terminus displays a COS domain, a Fibronectin type III repeat and a PRY-SPRY domain as all C-I sub-family TRIM members (Reymond et al., 2001; Short and Cox, 2006; Table 1). While the Fibronectin type III repeat and PRY-SPRY domain role in MID proteins is unclear, the COS domain was shown to mediate MID1 and MID2 association with the microtubular apparatus (Buchner et al., 1999; Cainarca et al., 1999; Short and Cox, 2006). Microtubular binding of MID1 is detectable also during mitosis and on the central spindle and midbody during cytokinesis (Cainarca et al., 1999). Recently, localisation at the midbody was reported also for MID2 (Gholkar et al., 2016). Whether MID proteins co-localise at the midbody in a mutual manner is still not unravelled. The coiled-coil region of MID1, besides mediating self-interaction, is also responsible for hetero-interaction with MID2 (Short et al., 2002; Meroni and Diez-Roux, 2005). The extent and stoichiometry of MID1/MID2 interaction is at present not known but one can envisage functions elicited by either homo- or hetero-complexes resulting in partial functional redundancy between MID proteins. Indeed, redundancy between the chicken orthologues of MID genes, cMid1, and cMid2, has been reported during the determination of avian left/right axis (Granata et al., 2005). Intriguingly, both human genes are implicated in genetic diseases: MID1 is mutated in patients presenting a complex neurodevelopmental disorder, the X-linked Opitz G/BBB syndrome (OS) (OMIM 300000) (Quaderi et al., 1997); and MID2 is mutated in an X-linked intellectual disability (OMIM 300928) (Geetha et al., 2014). This further suggests MID1 and MID2 overlapping functions. Along the same line, analyses of these genes during embryonic development in human, mouse and chicken show partial overlapping expression. MID1 is mainly found in the central nervous system (CNS), the developing branchial arches, the gastrointestinal and the urogenital systems, and the developing heart correlating with the tissues affected in OS (Dal Zotto et al., 1998; Richman et al., 2002; Pinson et al., 2004). MID2 displays low embryonic expression in the developing CNS, thymus and heart (Buchner et al., 1999). On the contrary, in human adult tissues, MID1 and MID2 have a distinct expression pattern: MID2 is mainly expressed in thyroid, smooth muscle, prostate, breast, and ovary whereas MID1 is found in the cerebellum, lung, colon, urinary bladder, prostate, placenta, breast, and ovary and retina (source ^1,2 ).

Regarding their E3 ligase function, in vitro activity for both MID1 and MID2 has been described (Han et al., 2011; Napolitano et al., 2011). In more physiological contexts, both unique and common MID proteins partners have been identified, some of which are reported as MID E3 ligases bona fide substrates. These data are briefly summarised in Table 1 and recently thoroughly reviewed in Li et al. (2016); Winter et al. (2016). These findings suggest that the two TRIM paralogues evolved maintaining common roles while developing their own specificity, likely in a context-specific manner. Their expression analyses during embryonic development revealed a preference for mitotically active compartments suggesting a role during cell cycle progression and here below we will discuss recent findings that support a role of MID1 and MID2 during the cytokinetic process.

MID1 and MID2 Involvement in Cytokinesis

As mentioned above, recent reports suggest an involvement of MID1 and MID2 in cytokinesis. Indeed, in HeLa cells, the depletion of either MID1 or MID2 leads to cell division defects, namely, cytokinetic arrest often followed by cell death and delay or failure to divide with regression into binucleated cells (Gholkar et al., 2016). This role is likely elicited through the interaction with several partners that we discuss here below.

Astrin

A recent work uncovered that both MID1 and MID2 bind the microtubule-associated protein Astrin (also known as SPAG5) (Gholkar et al., 2016). Astrin is important in the regulation of mitotic progression since its depletion causes centrosome instability and mitotic spindle malformation in HeLa cells. Astrin associates with the spindle throughout mitosis allowing chromosome alignment and segregation (Mack and Compton, 2001; Gruber et al., 2002). Diverse kinases, such as GSK3, Aurora A and Plk1, phosphorylate Astrin to regulate its mitotic function during spindle assembly (Cheng et al., 2008; Chiu et al., 2014; Chung et al., 2016).

The interaction between Astrin and the two TRIM proteins occurs independently from the cell cycle but has consequences only on cytokinesis. MID1 and MID2 partially co-localise with Astrin at the midbody of dividing cells. Interestingly, MID2 alone promotes Astrin ubiquitination at a unique site (K409) at mitotic exit targeting the protein to proteasomal degradation. Inappropriate accumulation of Astrin at the midbody provokes cytokinetic arrest, increased binucleation and cell death. Consistently, MID2 depletion leads to minor defects in early mitosis and major defects in cytokinesis supporting the importance of its E3 ligase activity in regulating Astrin degradation (Gholkar et al., 2016).

Unexpectedly, also MID1-deprived cells display division defects, including cytokinetic arrest, delayed or aborted abscission, inducing cell binucleation or death (Gholkar et al., 2016). At present it is not known if the observed cytokinetic phenotype is related to the lack of MID1-Astrin association and which is the mechanism involved. Further, whether MID1, MID2, and Astrin form a single protein complex is still undefined. An intriguing possibility is that distinct and dynamic homo- or hetero-MID complexes exist to target not only Astrin but also other cytokinesis-related proteins.

Alpha4/PP2Ac

The first reported target of MID1 E3 ligase activity was the catalytic subunit of serine/threonine protein phosphatase 2A (PP2Ac) driven to ubiquitin-mediated proteasomal degradation (Trockenbacher et al., 2001). MID1 directly interacts through the B-box 1 domain with Alpha4 (α4) that is one of the atypical regulatory subunits of PP2A (Nanahoshi et al., 1998; Liu et al., 2001; Trockenbacher et al., 2001; Short et al., 2002; LeNoue-Newton et al., 2011). Later on, α4 was reported to be a MID1 substrate as well (Watkins et al., 2012; Du et al., 2013). Active PP2A is a heterotrimeric holoenzyme composed of a catalytic (C subunit), a scaffold (A subunit) and a variable regulatory subunit (B, B′, B′′, or B′′′ subunits) that dictate substrate selectivity and subcellular localisation of the phosphatase holoenzyme. A small pool of PP2Ac was shown to form a complex containing α4 instead of the B subunit (Baskaran and Velmurugan, 2018). At cytokinesis, PP2Ac, A and B′γ1 subunits are all localised at the midbody in HeLa cells (Wu et al., 2017). In addition, PP2A-B′ holoenzyme counteracts Aurora B kinase activity controlling the length of spindle midzone through KIF4A dephosphorylation (Bastos et al., 2014).

The mechanism of self-regulation of the MID1/α4/PP2Ac complex involves a series of ubiquitination and dephosphorylation events that have been long studied but still remain to be completely unravelled. Initially, α4 was described to protect PP2Ac from degradation. Although in vitro assays suggested that MID1 catalyses mono- and di-ubiquitination of PP2Ac it is likely that other E3 ligases synergistically or alternatively are required to target its proteasomal degradation (Watkins et al., 2012; Du et al., 2014). Interestingly, MID1 not only targets a sub-pool of α4 for poly-ubiquitination-mediated degradation but also mono-ubiquitinates α4, triggering calpain mediated cleavage and degradation of its C-terminus containing the MID1 binding region (Watkins et al., 2012; Du et al., 2013). Whatever the mechanism, α4 cleavage disrupts the MID1/α4/PP2Ac complex, influencing PP2Ac stability (Winter et al., 2016). Altered PP2Ac activity affects mTORC1 complex formation and signalling (Liu et al., 2011). This pathway can play a significant role in the pathogenesis of OS and it would be interesting to investigate a possible MID1-mediated mTORC1 involvement in cytokinesis.

MID proteins contain two conserved phosphorylation consensus sites (Ser92 and Ser96) for GSK3 and MAPK, respectively (Short et al., 2002). Interestingly, MID1 interaction with α4 results in PP2Ac recruitment on microtubules and MID1 dephosphorylation at Ser96 (Aranda-Orgilles et al., 2008a). It is tempting to speculate that a similar regulatory mechanism involves MID2 as it binds α4 as well (Short et al., 2002). A fine balance of MID1 phosphorylation and dephosphorylation via MAPK and PP2A is important for regulating its affinity and its bi-directional movement along the microtubule network through kinesins and dyneins (Liu et al., 2001; Trockenbacher et al., 2001; Aranda-Orgilles et al., 2008a). Whether MID1 phosphorylation status affects E3 ligase activity or influences the interaction with α4 have not been addressed yet.

These findings leave some questions open and some issues still controversial. Indeed, α4 was shown to serve as a binding partner of PP2Ac rendering the latter catalytically inactive to avoid improper protein dephosphorylation. Then, when needed, α4 contributes to stabilise newly synthetised PP2Ac preventing its ubiquitin-mediated degradation thus permitting its assembly into functional PP2A holoenzymes (Kong et al., 2009). It is possible that binding of α4 to MID1 is needed to preserve a pool of newly available PP2Ac that can be transported along the microtubules to the spindle midzone. At this point, PP2Ac might become available for incorporation into active PP2A to dephosphorylate a pool of microtubule-associated phosphoproteins, such as KIF4A, to precisely control cytokinesis.

BRAF35

A recently identified MID1 substrate is the BRCA2-associated factor BRAF35 (also known as HMG20B) (Zanchetta et al., 2017) that was first isolated as part of a large nuclear multi-protein complex containing BRCA2 (Marmorstein et al., 2001).

MID1 ubiquitinates BRAF35 and is necessary for its turnover mainly outside the nucleus. Strikingly, although BRAF35 protein levels are regulated by the proteasome, atypical linkages are preferred in MID1-mediated ubiquitination, involving K6, K27, and K29 poly-ubiquitin chains. Among them, only K6 poly-ubiquitination promotes BRAF35 proteasomal degradation while K27 and K29 chains have no degradative effects (Zanchetta et al., 2017). The present knowledge does not offer insights to infer the effect of these modifications on BRAF35 (Kulathu and Komander, 2012). Of note, MID1 and BRAF35 co-localise in the cytoplasmic compartment and BRAF35 accumulates in larger cytoplasmic bodies when MID1 is depleted from HeLa cells (Zanchetta et al., 2017).

Recently BRAF35 was found in a region of the midbody compatible with MID1 localisation (Cainarca et al., 1999; Lee and Venkitaraman, 2014; Gholkar et al., 2016). Consistently, BRAF35 also associates with the previously mentioned PP2A target KIF4A, a motor protein that is essential for central spindle midzone and midbody organisation (Lee and Kim, 2003). Direct interaction between the cargo domain of KIF4A and BRCA2 was also proved, suggesting the existence of a multi-protein complex in which also BRAF35 takes part (Wu et al., 2008). BRCA2 is recruited on the midbody by the actin-binding protein Filamin A and is necessary for correct localisation of other midbody proteins, such as MKLP1, MKLP2, and PRC1 (Mondal et al., 2012). On the midbody BRCA2 forms a complex with CEP55, Alix, and Tsg101 and is required for the recruitment of the ESCRT machinery, necessary for abscission (Mondal et al., 2012). Depletion of BRAF35 from HeLa cells results in a delayed transition from anaphase to the completion of cell division (Lee et al., 2011). About half of BRAF35-depleted cells start cleavage furrowing but fail to divide, becoming binucleated (Lee et al., 2011). Interestingly, the same phenotype had previously been observed in BRCA2-deficient cells (Daniels et al., 2004). It is interesting that MID1 depletion phenocopies the cytokinesis failure-derived defects that were observed in the absence of BRAF35 or BRCA2 (Gholkar et al., 2016).

The C-terminal portion of BRAF35 spanning aa 173–276 is the minimal fragment required for BRCA2 binding and is also sufficient for its midbody localisation (Lee and Venkitaraman, 2014). However, contrary to the entire C-terminal fragment (aa 173–317), expression of the 173–276 aa fragment fails to restore cytokinesis in BRAF35-depleted cells suggesting the need of an additional factor (Lee and Venkitaraman, 2014). MID1 could represent such interactor, as the binding to BRAF35 occurs in an overlapping region (aa 230–317) to that necessary to form the BRAF35/BRCA2 complex, thus contributing to proper cytokinesis (Zanchetta et al., 2017).

The findings discussed here support the role of MID proteins in cell division through activities on multiple targets likely not only promoting their proteasomal degradation. However, it is still not clear to what extent their E3 ligase activity on the substrates cited in this review and graphically summarised in Figure 1 are interconnected. In this model, KIF4A might play a central role in the MID1- and MID2-regulated network. It is a matter of fact that both MID1 and MID2 are needed for successful cytokinesis with consequences in physiological and pathological conditions.

FIGURE 1

Conclusion

Although tightly regulated, cytokinesis lacks an effective checkpoint to ensure its fidelity. Cytokinesis can fail at different steps, because cleavage furrow ingression is inhibited or incomplete, or abscission is defective. The originated cells show increased chromosomal instability resulting in the generation of multipolar spindles and chromosome segregation defects (Sagona and Stenmark, 2010). Errors in cytokinesis may thus have dramatic consequences ranging from embryonic defects to cancer. Aberrant expression of cytokinesis regulators is indeed largely associated with many cancer types (Lens and Medema, 2019).

Recent findings showed elevated MID1 expression in prostate cancer and alteration of the MID1/a4/PP2A axis in lung adenocarcinoma and MID1 expression levels positively correlate with tumour Gleason scores (Kohler et al., 2014; Zhang et al., 2018). Similarly, high level of MID2 expression was significantly correlated with breast cancer progression (Wang et al., 2016). On the contrary, down-regulation of MID1 mediated by miR-135b has been shown to promote tumour progression of mammary carcinomas (Arigoni et al., 2013). Of note, high levels of Astrin have been described in cervical, pancreatic, hepatocellular carcinoma, and non-small-cell lung cancers (Valk et al., 2010; Yuan et al., 2014; Ansari et al., 2015; Liu et al., 2018; Yang et al., 2018). In the case of BRAF35, the A247P mutation reported in a case of lung carcinoma was shown to interfere with its midbody localisation and BRCA2 binding (Lee and Venkitaraman, 2014). This mutation induces cytokinesis failure through a dominant negative mechanism possibly affecting MID1 activity. Thus, it appears that dysregulation of MID1 plays a role in tumourigenesis, likely affecting factors that control somatic cell proliferation.

Clinically, MID1 and MID2 are implicated also in genetic developmental disorders (Quaderi et al., 1997; Geetha et al., 2014). Their involvement in cytokinesis does not come as a surprise as embryonic development is the organism phase with the highest mitotic index. During development, aberrant cytokinesis can have a strong impact not only on cell proliferation but also on morphogenetic processes. In fact, inherited midbodies can affect cell polarity and cell communication and, in epithelia, midbody positioning influences planar tissue architecture (Herszterg et al., 2014; Bernabe-Rubio et al., 2016; Antanaviciute et al., 2018).

The identification of the involvement of MID1 and MID2 in cytokinesis is intriguingly though we are still far from clarifying the precise dynamics of the occurring events. Further investigations will be necessary to understand the dynamics of the complexes containing MID proteins and Astrin, BRAF35 and PP2A and their interplay, if any. The future dissection of these mechanisms, together with parallel in vivo studies, will be necessary to get a comprehensive picture and for future clinical application.

References

• 1

  AgromayorM.Martin-SerranoJ. (2013). Knowing when to cut and run: mechanisms that control cytokinetic abscission.Trends Cell Biol.23433–441. 10.1016/j.tcb.2013.04.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 2

  AnsariD.AnderssonR.BaudenM. P.AnderssonB.ConnollyJ. B.WelinderC.et al (2015). Protein deep sequencing applied to biobank samples from patients with pancreatic cancer.J. Cancer Res. Clin. Oncol.141369–380. 10.1007/s00432-014-1817-x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 3

  AntanaviciuteI.GibiezaP.PrekerisR.SkeberdisV. A. (2018). Midbody: from the regulator of cytokinesis to postmitotic signaling organelle.Medicina54:E53. 10.3390/medicina54040053

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 4

  Aranda-OrgillesB.AignerJ.KunathM.LurzR.SchneiderR.SchweigerS. (2008a). Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A.PLoS One3:e3507. 10.1371/journal.pone.0003507

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 5

  Aranda-OrgillesB.TrockenbacherA.WinterJ.AignerJ.KohlerA.JastrzebskaE.et al (2008b). The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex.Hum. Genet.123163–176. 10.1007/s00439-007-0456-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 6

  ArigoniM.BarutelloG.RiccardoF.ErcoleE.CantarellaD.OrsoF.et al (2013). miR-135b coordinates progression of ErbB2-driven mammary carcinomas through suppression of MID1 and MTCH2.Am. J. Pathol.1822058–2070. 10.1016/j.ajpath.2013.02.046

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 7

  BaskaranR.VelmuruganB. K. (2018). Protein phosphatase 2A as therapeutic targets in various disease models.Life Sci.21040–46. 10.1016/j.lfs.2018.08.063

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 8

  BastosR. N.CundellM. J.BarrF. A. (2014). KIF4A and PP2A-B56 form a spatially restricted feedback loop opposing Aurora B at the anaphase central spindle.J. Cell Biol.207683–693. 10.1083/jcb.201409129

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 9

  BellJ. L.MalyukovaA.HolienJ. K.KoachJ.ParkerM. W.KavallarisM.et al (2012). TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members.PLoS One7:e37470. 10.1371/journal.pone.0037470

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 10

  Bernabe-RubioM.AndresG.Casares-AriasJ.Fernandez-BarreraJ.RangelL.Reglero-RealN.et al (2016). Novel role for the midbody in primary ciliogenesis by polarized epithelial cells.J. Cell Biol.214259–273. 10.1083/jcb.201601020

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 11

  BertiC.FontanellaB.FerrentinoR.MeroniG. (2004). Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules.BMC Cell Biol.5:9. 10.1186/1471-2121-5-9

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 12

  BuchnerG.MontiniE.AndolfiG.QuaderiN.CainarcaS.MessaliS.et al (1999). MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.Hum. Mol. Genet.81397–1407. 10.1093/hmg/8.8.1397

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 13

  CainarcaS.MessaliS.BallabioA.MeroniG. (1999). Functional characterization of the Opitz syndrome gene product (midin): evidence for homodimerization and association with microtubules throughout the cell cycle.Hum. Mol. Genet.81387–1396. 10.1093/hmg/8.8.1387

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 14

  ChengT. S.HsiaoY. L.LinC. C.YuC. T.HsuC. M.ChangM. S.et al (2008). Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.J. Biol. Chem.2832454–2464. 10.1074/jbc.M706794200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 15

  ChiuS. C.ChenJ. M.WeiT. Y.ChengT. S.WangY. H.KuC. F.et al (2014). The mitosis-regulating and protein-protein interaction activities of astrin are controlled by aurora-A-induced phosphorylation.Am. J. Physiol. Cell Physiol.307C466–C478. 10.1152/ajpcell.00164.2014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 16

  ChungH. J.ParkJ. E.LeeN. S.KimH.JangC. Y. (2016). Phosphorylation of astrin regulates its kinetochore function.J. Biol. Chem.29117579–17592. 10.1074/jbc.M115.712745

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 17

  Dal ZottoL.QuaderiN. A.ElliottR.LingerfelterP. A.CarrelL.ValsecchiV.et al (1998). The mouse Mid1 gene: implications for the pathogenesis of Opitz syndrome and the evolution of the mammalian pseudoautosomal region.Hum. Mol. Genet.7489–499. 10.1093/hmg/7.3.489

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 18

  DanielsM. J.WangY.LeeM.VenkitaramanA. R. (2004). Abnormal cytokinesis in cells deficient in the breast cancer susceptibility protein BRCA2.Science306876–879. 10.1126/science.1102574

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 19

  D’AvinoP. P.GiansantiM. G.PetronczkiM. (2015). Cytokinesis in animal cells.Cold Spring Harb. Perspect. Biol.7:a015834. 10.1101/cshperspect.a015834

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 20

  DuH.HuangY.ZaghlulaM.WaltersE.CoxT. C.MassiahM. A. (2013). The MID1 E3 ligase catalyzes the polyubiquitination of Alpha4 (alpha4), a regulatory subunit of protein phosphatase 2A (PP2A): novel insights into MID1-mediated regulation of PP2A.J. Biol. Chem.28821341–21350. 10.1074/jbc.M113.481093

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 21

  DuH.WuK.DidoronkuteA.LevyM. V.TodiN.ShchelokovaA.et al (2014). MID1 catalyzes the ubiquitination of protein phosphatase 2A and mutations within its Bbox1 domain disrupt polyubiquitination of alpha4 but not of PP2Ac.PLoS One9:e107428. 10.1371/journal.pone.0107428

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 22

  EchardA.HicksonG. R.FoleyE.O’FarrellP. H. (2004). Terminal cytokinesis events uncovered after an RNAi screen.Curr. Biol.141685–1693. 10.1016/j.cub.2004.08.063

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 23

  EggertU. S.MitchisonT. J.FieldC. M. (2006). Animal cytokinesis: from parts list to mechanisms.Annu. Rev. Biochem.75543–566. 10.1146/annurev.biochem.74.082803.133425

  • CrossRef
  • Google Scholar
  • View reference in article

• 24

  GeethaT. S.MichealrajK. A.KabraM.KaurG.JuyalR. C.ThelmaB. K. (2014). Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India.Hum. Mutat.3541–44. 10.1002/humu.22453

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 25

  GholkarA. A.SeneseS.LoY. C.VidesE.ContrerasE.HodaraE.et al (2016). The X-linked-intellectual-disability-associated ubiquitin ligase Mid2 interacts with astrin and regulates astrin levels to promote cell division.Cell Rep.14180–188. 10.1016/j.celrep.2015.12.035

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 26

  GilbertoS.PeterM. (2017). Dynamic ubiquitin signaling in cell cycle regulation.J. Cell Biol.2162259–2271. 10.1083/jcb.201703170

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 27

  GranataA.SaveryD.HazanJ.CheungB. M.LumsdenA.QuaderiN. A. (2005). Evidence of functional redundancy between MID proteins: implications for the presentation of Opitz syndrome.Dev. Biol.277417–424. 10.1016/j.ydbio.2004.09.036

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 28

  GruberJ.HarborthJ.SchnabelJ.WeberK.HatzfeldM. (2002). The mitotic-spindle-associated protein astrin is essential for progression through mitosis.J. Cell Sci.1154053–4059. 10.1242/jcs.00088

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 29

  HanX.DuH.MassiahM. A. (2011). Detection and characterization of the in vitro E3 ligase activity of the human MID1 protein.J. Mol. Biol.407505–520. 10.1016/j.jmb.2011.01.048

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 30

  HatakeyamaS. (2017). TRIM family proteins: roles in autophagy, immunity, and carcinogenesis.Trends Biochem. Sci.42297–311. 10.1016/j.tibs.2017.01.002

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 31

  HersztergS.PinheiroD.BellaicheY. (2014). A multicellular view of cytokinesis in epithelial tissue.Trends Cell Biol.24285–293. 10.1016/j.tcb.2013.11.009

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 32

  KohlerA.DemirU.KicksteinE.KraussS.AignerJ.Aranda-OrgillesB.et al (2014). A hormone-dependent feedback-loop controls androgen receptor levels by limiting MID1, a novel translation enhancer and promoter of oncogenic signaling.Mol. Cancer13:146. 10.1186/1476-4598-13-146

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 33

  KomanderD.RapeM. (2012). The ubiquitin code.Annu. Rev. Biochem.81203–229. 10.1146/annurev-biochem-060310-170328

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 34

  KongM.DitsworthD.LindstenT.ThompsonC. B. (2009). Alpha4 is an essential regulator of PP2A phosphatase activity.Mol. Cell3651–60. 10.1016/j.molcel.2009.09.025

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 35

  KrupinaK.KleissC.MetzgerT.FournaneS.SchmuckerS.HofmannK.et al (2016). Ubiquitin receptor protein UBASH3B drives aurora B recruitment to mitotic microtubules.Dev. Cell3663–78. 10.1016/j.devcel.2015.12.017

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 36

  KulathuY.KomanderD. (2012). Atypical ubiquitylation - the unexplored world of polyubiquitin beyond Lys48 and Lys63 linkages.Nat. Rev. Mol. Cell Biol.13508–523. 10.1038/nrm3394

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 37

  LeeM.DanielsM. J.GarnettM. J.VenkitaramanA. R. (2011). A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor.Oncogene303360–3369. 10.1038/onc.2011.55

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 38

  LeeM.VenkitaramanA. R. (2014). A cancer-associated mutation inactivates a region of the high-mobility group protein HMG20b essential for cytokinesis.Cell Cycle132554–2563. 10.4161/15384101.2014.942204

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 39

  LeeY. M.KimW. (2003). Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.Biochem. J.374497–503. 10.1042/bj20030452

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 40

  LenihanJ. A.SahaO.YoungP. W. (2017). Proteomic analysis reveals novel ligands and substrates for LNX1 E3 ubiquitin ligase.PLoS One12:e0187352. 10.1371/journal.pone.0187352

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 41

  LeNoue-NewtonM.WatkinsG. R.ZouP.GermaneK. L.McCorveyL. R.WadzinskiB. E.et al (2011). The E3 ubiquitin ligase- and protein phosphatase 2A (PP2A)-binding domains of the Alpha4 protein are both required for Alpha4 to inhibit PP2A degradation.J. Biol. Chem.28617665–17671. 10.1074/jbc.M111.222414

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 42

  LensS. M. A.MedemaR. H. (2019). Cytokinesis defects and cancer.Nat. Rev. Cancer1932–45. 10.1038/s41568-018-0084-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 43

  LiB.ZhouT.ZouY. (2016). Mid1/Mid2 expression in craniofacial development and a literature review of X-linked opitz syndrome.Mol. Genet. Genomic Med.495–105. 10.1002/mgg3.183

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 44

  LiY.JungeJ. A.ArnesanoC.GrossG. G.MinerJ. H.MoatsR.et al (2018). Discs large 1 controls daughter-cell polarity after cytokinesis in vertebrate morphogenesis.Proc. Natl. Acad. Sci. U.S.A.115E10859–E10868. 10.1073/pnas.1713959115

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 45

  LiuE.KnutzenC. A.KraussS.SchweigerS.ChiangG. G. (2011). Control of mTORC1 signaling by the Opitz syndrome protein MID1.Proc. Natl. Acad. Sci. U.S.A.1088680–8685. 10.1073/pnas.1100131108

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 46

  LiuH.HuJ.WeiR.ZhouL.PanH.ZhuH.et al (2018). SPAG5 promotes hepatocellular carcinoma progression by downregulating SCARA5 through modifying beta-catenin degradation.J. Exp. Clin. Cancer Res.37:229. 10.1186/s13046-018-0891-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 47

  LiuJ.PrickettT. D.ElliottE.MeroniG.BrautiganD. L. (2001). Phosphorylation and microtubule association of the Opitz syndrome protein mid-1 is regulated by protein phosphatase 2A via binding to the regulatory subunit alpha 4.Proc. Natl. Acad. Sci. U.S.A.986650–6655. 10.1073/pnas.111154698

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 48

  MackG. J.ComptonD. A. (2001). Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein.Proc. Natl. Acad. Sci. U.S.A.9814434–14439. 10.1073/pnas.261371298

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 49

  MarmorsteinL. Y.KinevA. V.ChanG. K.BocharD. A.BeniyaH.EpsteinJ. A.et al (2001). A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression.Cell104247–257. 10.1016/S0092-8674(01)00209-4

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 50

  MeroniG.Diez-RouxG. (2005). TRIM/RBCC, a novel class of ‘single protein RING finger’ E3 ubiquitin ligases.Bioessays271147–1157. 10.1002/bies.20304

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 51

  MondalG.RowleyM.GuidugliL.WuJ.PankratzV. S.CouchF. J. (2012). BRCA2 localization to the midbody by filamin A regulates cep55 signaling and completion of cytokinesis.Dev. Cell23137–152. 10.1016/j.devcel.2012.05.008

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 52

  NanahoshiM.NishiumaT.TsujishitaY.HaraK.InuiS.SakaguchiN.et al (1998). Regulation of protein phosphatase 2A catalytic activity by alpha4 protein and its yeast homolog Tap42.Biochem. Biophys. Res. Commun.251520–526. 10.1006/bbrc.1998.9493

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 53

  NapolitanoL. M.JaffrayE. G.HayR. T.MeroniG. (2011). Functional interactions between ubiquitin E2 enzymes and TRIM proteins.Biochem. J.434309–319. 10.1042/BJ20101487

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 54

  PfirrmannT.JandtE.RanftS.LokapallyA.NeuhausH.PerronM.et al (2016). Hedgehog-dependent E3-ligase Midline1 regulates ubiquitin-mediated proteasomal degradation of Pax6 during visual system development.Proc. Natl. Acad. Sci. U.S.A.11310103–10108. 10.1073/pnas.1600770113

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 55

  PinsonL.AugeJ.AudollentS.MatteiG.EtcheversH.GigarelN.et al (2004). Embryonic expression of the human MID1 gene and its mutations in Opitz syndrome.J. Med. Genet.41381–386. 10.1136/jmg.2003.014829

  • CrossRef
  • Google Scholar
  • View reference in article

• 56

  PohlC.JentschS. (2009). Midbody ring disposal by autophagy is a post-abscission event of cytokinesis.Nat. Cell Biol.1165–70. 10.1038/ncb1813

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 57

  QuaderiN. A.SchweigerS.GaudenzK.FrancoB.RugarliE. I.BergerW.et al (1997). Opitz G/BBB syndrome, a defect of midline development, is due to mutations in a new RING finger gene on Xp22.Nat. Genet.17285–291. 10.1038/ng1197-285

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 58

  ReymondA.MeroniG.FantozziA.MerlaG.CairoS.LuziL.et al (2001). The tripartite motif family identifies cell compartments.EMBO J.202140–2151. 10.1093/emboj/20.9.2140

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 59

  RichmanJ. M.FuK. K.CoxL. L.SibbonsJ. P.CoxT. C. (2002). Isolation and characterisation of the chick orthologue of the Opitz syndrome gene, Mid1, supports a conserved role in vertebrate development.Int. J. Dev. Biol.46441–448.

  • Pubmed Abstract
  • Google Scholar
  • View reference in article

• 60

  RollandT.TasanM.CharloteauxB.PevznerS. J.ZhongQ.SahniN.et al (2014). A proteome-scale map of the human interactome network.Cell1591212–1226. 10.1016/j.cell.2014.10.050

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 61

  SagonaA. P.StenmarkH. (2010). Cytokinesis and cancer.FEBS Lett.5842652–2661. 10.1016/j.febslet.2010.03.044

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 62

  SardielloM.CairoS.FontanellaB.BallabioA.MeroniG. (2008). Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties.BMC Evol. Biol.8:225. 10.1186/1471-2148-8-225

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 63

  SchweigerS.DornS.FuchsM.KohlerA.MatthesF.MullerE. C.et al (2014). The E3 ubiquitin ligase MID1 catalyzes ubiquitination and cleavage of Fu.J. Biol. Chem.28931805–31817. 10.1074/jbc.M113.541219

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 64

  ShortK. M.CoxT. C. (2006). Sub-classification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding.J. Biol. Chem.2818970–8980. 10.1074/jbc.M512755200

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 65

  ShortK. M.HopwoodB.YiZ.CoxT. C. (2002). MID1 and MID2 homo- and heterodimerise to tether the rapamycin- sensitive PP2A regulatory subunit, Alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders.BMC Cell Biol.3:1. 10.1186/1471-2121-3-1

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 66

  TeixeiraL. K.ReedS. I. (2013). Ubiquitin ligases and cell cycle control.Annu. Rev. Biochem.82387–414. 10.1146/annurev-biochem-060410-105307

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 67

  TrockenbacherA.SuckowV.FoersterJ.WinterJ.KraussS.RopersH. H.et al (2001). MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation.Nat. Genet.29287–294. 10.1038/ng762

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 68

  ValkK.VooderT.KoldeR.ReintamM. A.PetzoldC.ViloJ.et al (2010). Gene expression profiles of non-small cell lung cancer: survival prediction and new biomarkers.Oncology79283–292. 10.1159/000322116

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 69

  WangL.WuJ.YuanJ.ZhuX.WuH.LiM. (2016). Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro and in vivo.Front. Med.1041–51. 10.1007/s11684-016-0429-z

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 70

  WatkinsG. R.WangN.MazalouskasM. D.GomezR. J.GuthrieC. R.KraemerB. C.et al (2012). Monoubiquitination promotes calpain cleavage of the protein phosphatase 2A (PP2A) regulatory subunit alpha4, altering PP2A stability and microtubule-associated protein phosphorylation.J. Biol. Chem.28724207–24215. 10.1074/jbc.M112.368613

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 71

  WinterJ.BasilicataM. F.StemmlerM. P.KraussS. (2016). The MID1 protein is a central player during development and in disease.Front. Biosci.21664–682. 10.2741/4413

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 72

  WuC. G.ChenH.GuoF.YadavV. K.McIlwainS. J.RowseM.et al (2017). PP2A-B’ holoenzyme substrate recognition, regulation and role in cytokinesis.Cell Discov.3:17027. 10.1038/celldisc.2017.27

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 73

  WuG.ZhouL.KhidrL.GuoX. E.KimW.LeeY. M.et al (2008). A novel role of the chromokinesin Kif4A in DNA damage response.Cell Cycle72013–2020. 10.4161/cc.7.13.6130

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 74

  YangY. F.ZhangM. F.TianQ. H.FuJ.YangX.ZhangC. Z.et al (2018). SPAG5 interacts with CEP55 and exerts oncogenic activities via PI3K/AKT pathway in hepatocellular carcinoma.Mol. Cancer17:117. 10.1186/s12943-018-0872-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 75

  YuanL. J.LiJ. D.ZhangL.WangJ. H.WanT.ZhouY.et al (2014). SPAG5 upregulation predicts poor prognosis in cervical cancer patients and alters sensitivity to taxol treatment via the mTOR signaling pathway.Cell Death Dis.5:e1247. 10.1038/cddis.2014.222

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 76

  ZanchettaM. E.NapolitanoL. M. R.MaddaloD.MeroniG. (2017). The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.Biochim. Biophys. Acta Mol. Cell Res.18641844–1854. 10.1016/j.bbamcr.2017.07.014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 77

  ZhangL.LiJ.LvX.GuoT.LiW.ZhangJ. (2018). MID1-PP2A complex functions as new insights in human lung adenocarcinoma.J. Cancer Res. Clin. Oncol.144855–864. 10.1007/s00432-018-2601-0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar