Multiplex Genome Editing to Overcome Tumor Immune Evasion

Tumor immune evasion remains one of the greatest obstacles to achieving durable responses in cancer therapy. Tumors often employ multiple and overlapping mechanisms to escape immune recognition and destruction, such as downregulation of antigen presentation, modulation of immune checkpoints, and alteration of the tumor microenvironment. With advances in genome editing technologies—including CRISPR/Cas9, base editing, and prime editing—researchers now have powerful tools to simultaneously target and modify multiple genes, enabling a new generation of precision immunotherapies.

This Research Topic aims to showcase the latest developments and applications in multiplex genome editing designed to overcome tumor immune evasion. We welcome original research articles, reviews, mini-reviews, methods papers, and perspectives across a range of tumor types and immune cell platforms. Topics of interest include, but are not limited to:

1. Novel genome editing strategies to simultaneously target multiple immune evasion pathways in cancer cells
2. Engineering immune cells (e.g., T cells, NK cells, macrophages) using multiplex gene editing to enhance anti-tumor activity
3. Mechanisms and models of tumor immune evasion addressed through multiplex editing approaches
4. Preclinical and clinical data on multiplex-edited immune cell therapies
5. Delivery systems and technologies for efficient multiplex editing
6. Ethical, safety, and regulatory considerations for multiplexed gene editing in cancer therapy

We invite contributions that advance mechanistic insight, translational research, and future perspectives in this rapidly evolving field. The goal is to accelerate innovative solutions that can effectively break down the barriers of immune resistance and improve patient outcomes in cancer treatment.

Article types and fees

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• FAIR² DATA Direct Submission
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• ... View all formats

Articles that are accepted for publication by our external editors following rigorous peer review incur a publishing fee charged to Authors, institutions, or funders.

Article types

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• FAIR² DATA Direct Submission
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• Original Research
• Perspective
• Policy and Practice Reviews
• Policy Brief
• Review
• Systematic Review

Keywords: Multiplex Genome Editing, Tumor Immune Evasion, CRISPR/Cas9, Immunotherapy, Cancer Resistance

Important note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.