Next-Generation CRISPR Systems: Expanding the Genome Editing Toolbox

Since the discovery of CRISPR-Cas9, genome editing has been revolutionized by the ability to introduce targeted genetic changes with unprecedented precision. However, the limitations of first-generation CRISPR systems—such as off-target effects, PAM sequence constraints, and delivery challenges—have driven researchers to develop new and improved tools. Next-generation CRISPR systems encompass a diverse array of technologies, including novel Cas proteins (e.g., Cas12, Cas13, CasX), base editors, prime editors, and epigenome-editing platforms.

This Research Topic invites exploration of the molecular mechanisms, engineering advancements, and innovative applications of next-generation CRISPR tools. Key areas of interest include the discovery and characterization of novel Cas nucleases with unique properties, the development of highly specific and versatile editors for DNA and RNA, strategies to overcome existing technical challenges, and the deployment of these tools in basic research, biotechnology, medicine, and agriculture.

We welcome the following themes, but are not limited to:

1. Discovery and characterization of novel Cas proteins with unique target recognition and editing capabilities.
2. Development and optimization of base and prime editing technologies for greater precision and efficiency.
3. Strategies to improve editing specificity and reduce off-target effects.
4. Innovative delivery systems for efficient CRISPR component introduction into diverse cell types or organisms.
5. Applications of next-generation CRISPR in disease modeling and therapeutic gene correction.
6. Advances in agricultural biotechnology using new CRISPR systems for crop improvement.
7. Ethical, safety, and regulatory analyses related to emerging genome editing technologies.

By expanding the genome editing toolbox, these next-generation systems hold promise for more precise, efficient, and versatile genetic manipulation—paving the way for transformative applications in gene therapy, functional genomics, synthetic biology, and beyond.

Article types and fees

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• Original Research
• ... View all formats

Articles that are accepted for publication by our external editors following rigorous peer review incur a publishing fee charged to Authors, institutions, or funders.

Article types

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• Original Research
• Perspective
• Policy and Practice Reviews
• Policy Brief
• Review
• Systematic Review

Keywords: Genome Editing, CRISPR Technologies, Cas Proteins, Base Editing, Gene Therapy

Important note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.