Advances in Genome Editing for Translational Research in Large Animal Models

Advances in genome editing have transformed the development of animal models used to understand human diseases. While transgenic mouse models have broadly contributed to many insights of disease mechanisms, their translational limitations, such as small size, different physiology and organ structure, can make it challenging to fully represent human disease progression and treatment outcomes. As a result, large animal models (e.g., pigs, sheep, and goats) are increasingly recognized as better alternatives due to their closer anatomical and physiological resemblance to humans.

Recent advancements in CRISPR/Cas9-based genome editing technologies have revolutionized the scope of genetic modulation in animal research. This technology allows researchers to introduce precise and human-derived mutations into strategic genes, thereby replicating disease phenotypes observed in humans. CRISPR/Cas9 not only enhances the fidelity of genetic alterations but also optimizes the creation of more representative animal models. This progression is pivotal for deciphering gene regulation and underlying molecular mechanisms in various diseases, in addition to providing invaluable platforms for testing new therapies and evaluating their molecular impacts comprehensively.

This Research Topic focuses on innovations that advance the development and refinement of large animal models associated with various human diseases. It also seeks contributions to genomic modifications intended for the well-being and research related to these conditions. To gather further insights in this rapidly evolving field, we welcome articles addressing, but not limited to, the following themes:

o Large animal models of human diseases
o New gene editing platforms applied in large animal mammalian cells
o Gene editing applications for biomedical and agricultural purposes
o Application of new gene editing enhancers in cells and embryos
o Animal resistance to pathogens
o New gene editing and genetic engineering applications in animal cells
o Precision editing using viral or nanoparticle delivery in animal/human cells

We especially encourage the submission of Original Research articles covering these facets, which will further fortify the knowledge base and inspire innovation in genome editing applications.

Article types and fees

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• Original Research
• ... View all formats

Articles that are accepted for publication by our external editors following rigorous peer review incur a publishing fee charged to Authors, institutions, or funders.

Article types

This Research Topic accepts the following article types, unless otherwise specified in the Research Topic description:

• Brief Research Report
• Editorial
• FAIR² Data
• General Commentary
• Hypothesis and Theory
• Methods
• Mini Review
• Opinion
• Original Research
• Perspective
• Policy and Practice Reviews
• Policy Brief
• Review
• Systematic Review

Keywords: CRISPR/Cas9, Large animal models, translational research, precision genome editing, disease modeling, gene regulation, viral delivery, nanoparticle delivery, transgenic technologies, pathogen resistance, Biomedical applications, Agricultural applications

Important note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.