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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00284

Coding cell identity of human skeletal muscle progenitor cells using cell surface markers: Current status and remaining challenges for characterization and isolation

Sin-Ruow Tey1, Samantha Robertson1, Eileen Lynch2 and  Masatoshi Suzuki1*
  • 1University of Wisconsin-Madison, United States
  • 2Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, United States

Skeletal muscle progenitor cells (SMPCs), also called myogenic progenitors, have been studied extensively in recent years because of their promising therapeutic potential to preserve and recover skeletal muscle mass and function in patients with cachexia, sarcopenia and neuromuscular diseases. SMPCs can be utilized to investigate the mechanisms of natural and pathological myogenesis via in vitro modeling and in vivo experimentation. While various types of SMPCs are currently available from several sources, human pluripotent stem cells (PSCs) offer an efficient and cost-effective method to derive SMPCs. As human PSC-derived cells often display varying heterogeneity in cell types, cell enrichment using cell surface markers remains a critical step in current procedures to establish a pure population of SMPCs. Here we summarize the cell surface markers currently being used to detect human SMPCs, describing their potential application for characterizing, identifying and isolating human PSC-derived SMPCs. To date, several positive and negative markers have been used to enrich human SMPCs from differentiated PSCs for cell sorting. A careful analysis of current findings can broaden our understanding and reveal potential uses for these surface markers with SMPCs.

Keywords: skeletal muscle, Skeletal muscle progenitor cells, Stem Cells, Cell surface markers, Human Induced Pluripotent Stem Cells (iPSC), muscular dystrophy, Neuromuscular Diseases

Received: 28 Jun 2019; Accepted: 01 Nov 2019.

Copyright: © 2019 Tey, Robertson, Lynch and Suzuki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Masatoshi Suzuki, University of Wisconsin-Madison, Madison, 53715-1149, Alabama, United States,