Impact Factor 5.206 | CiteScore 4.82
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00289

Genome-wide analysis of circular RNAs mediated ceRNA regulation in porcine embryonic muscle development

 Linjun Hong1,  Ting Gu1, Yanjuan He1, Chen Zhou1, Qun Hu1,  Wang Xingwang1, Enqin Zheng1, Sixiu Huang1, Zheng Xu1,  Jie Yang1,  Huaqiang Yang1, Zicong Li1,  Dewu Liu1, Gengyuan Cai1* and  Zhenfang Wu1*
  • 1College of Animal Science, South China Agricultural University, China

Many circular RNAs (circRNAs) have been discovered in various tissues and cell types in pig. However, the temporal expression pattern of circRNAs during porcine embryonic muscle development remains unclear. Here, we present a panorama view of circRNA expression in embryonic muscle development at 33-, 65- and 90-days post-coitus (dpc) from Duroc pigs. An unbiased analysis reveals that more than 5000 circRNAs specifically express in embryonic muscle development. The amount and complexity of circRNA expression is most pronounced in skeletal muscle at day 33 of gestation. Our circRNAs annotation analyses show that “hot-spot” genes produce multiple circRNA isoforms and RNA binding protein (RBPs) may regulate the biogenesis of circRNAs. Furthermore, we observed that host genes of differentially expressed circRNA across porcine muscle development are enriched in skeletal muscle function. A ceRNA network analysis of circRNAs reveals that circRNAs regulate muscle gene expression by functioning as miRNA sponges. Finally, our experimental validation demonstrated that circTUT7 regulate the expression of HMG20B in a competing endogenous RNA (ceRNA) mechanism. Our analyses show that circRNAs are dynamically expressed and interacting with muscle genes through ceRNA manner, suggesting their critical functions in embryonic skeletal muscle development.

Keywords: porcine, CircRNAs, ceRNA, embryo, skeletal muscle

Received: 05 Aug 2019; Accepted: 05 Nov 2019.

Copyright: © 2019 Hong, Gu, He, Zhou, Hu, Xingwang, Zheng, Huang, Xu, Yang, Yang, Li, Liu, Cai and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Gengyuan Cai, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China, cgy0415@163.com
Prof. Zhenfang Wu, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China, wzfemail@163.com