Original Research ARTICLE
Definition of the anti-inflammatory Oligosaccharides derived from the Galactosaminogalactan (GAG) from Aspergillus fumigatus
- 1Institut Pasteur, France
- 2University of Perugia, Italy
- 3Lille University of Science and Technology, France
- 4University of Minho, Portugal
Galactosaminogalactan (GAG) is an insoluble aminosugar polymer produced by Aspergillus fumigatus and has anti-inflammatory properties. Here, the minimum glycosidic sequences required for the induction of IL-1Ra by peripheral blood mononuclear cells (PBMCs) was investigated. Using chemical degradation of native GAG to isolate soluble oligomers, we have found that the de-N-acetylation of galactosamine residues and the size of oligomer are critical for the in vitro immune response. A minimal oligomer size of 20 galactosamine residues is required for the anti-inflammatory response but the presence of galactose residues is not necessary. In a Dextran sulfate induced colitis mouse model, a fraction of de-N-acetylated oligomers of 13
Keywords: Galactosaminogalactan, Aspergillus fumigatus, IL-Ra, Anti-inflammatory response, Glycodrug
Received: 26 Aug 2019;
Accepted: 08 Oct 2019.
Copyright: © 2019 Fontaine, Gressler, Heddergott, N´go, Renga, Oikonomou, Moretti, Coddeville, Gaifem, Silvestre, Romani and Latgé. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Thierry Fontaine, Institut Pasteur, Paris, France, email@example.com