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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2019.00365

Definition of the anti-inflammatory Oligosaccharides derived from the Galactosaminogalactan (GAG) from Aspergillus fumigatus

 Thierry Fontaine1*, Markus Gressler1, Christoph Heddergott1, Inés C. N´go1,  Giorgia Renga2,  Vasilis Oikonomou2,  Silvia Moretti2, Bernadette Coddeville3,  Joana Gaifem4,  Ricardo Silvestre4,  Luigina Romani2 and Jean-Paul Latgé1
  • 1Institut Pasteur, France
  • 2University of Perugia, Italy
  • 3Lille University of Science and Technology, France
  • 4University of Minho, Portugal

Galactosaminogalactan (GAG) is an insoluble aminosugar polymer produced by Aspergillus fumigatus and has anti-inflammatory properties. Here, the minimum glycosidic sequences required for the induction of IL-1Ra by peripheral blood mononuclear cells (PBMCs) was investigated. Using chemical degradation of native GAG to isolate soluble oligomers, we have found that the de-N-acetylation of galactosamine residues and the size of oligomer are critical for the in vitro immune response. A minimal oligomer size of 20 galactosamine residues is required for the anti-inflammatory response but the presence of galactose residues is not necessary. In a Dextran sulfate induced colitis mouse model, a fraction of de-N-acetylated oligomers of 13

Keywords: Galactosaminogalactan, Aspergillus fumigatus, IL-Ra, Anti-inflammatory response, Glycodrug

Received: 26 Aug 2019; Accepted: 08 Oct 2019.

Copyright: © 2019 Fontaine, Gressler, Heddergott, N´go, Renga, Oikonomou, Moretti, Coddeville, Gaifem, Silvestre, Romani and Latgé. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Thierry Fontaine, Institut Pasteur, Paris, France, thierry.fontaine@pasteur.fr