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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2019.00369

Characteristics of Gut Microbiota in Patients with Rheumatoid Arthritis in Shanghai, China

 SUN YANG1,  Qian Chen2, Ping lin3, Rong Xu1,  Dongyi He1, Weiqing Ji1,  Bian Yanqin1, Yu Shen1, Qingtian Li2,  Chang Liu2,  KE DONG2,  Yi-Wei Tang4, Zhiheng Pei5,  Liying Yang5,  Hongzhou Lu6,  Lianbo Xiao1 and  Xiao-kui GUO2*
  • 1Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, China
  • 2School of Medicine, Shanghai Jiao Tong University, China
  • 3Shanghai Mental Health Center (SMHC), China
  • 4Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, United States
  • 5New York University, United States
  • 6Shanghai Public Health Clinical Center, Fudan University, China

Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts in China. This study aimed to identify differences in the fecal microbiomes of 66 Chinese patients with RA and 60 healthy Chinese controls. The V3-V4 variable regions of bacterial 16S rRNA genes were sequenced with the Illumina system to define the bacterial composition. The alpha-diversity index of the microbiome of the RA patients was significantly lower than that of the control group. The bacterial genera Bacteroides (p=0.02202) and Escherichia-Shigella (p=0.03137) were more abundant in RA patients. In contrast, Lactobacillus (p=0.000014), Alloprevotella (p=0.0000008615), Enterobacter (p=0.000005759), and Odoribacter (p=0.0000166) were less abundant in the RA group than in the control group. Spearman correlation analysis of blood physiological measures of RA showed that bacterial genera such as Dorea and Ruminococcus were positively correlated with RF-IgA and anti-CCP antibodies. Furthermore, Alloprevotella and Parabacteroides were positively correlated with the erythrocyte sedimentation rate, and Prevotella-2 and Alloprevotella were positively correlated with C-reactive protein, both biomarkers of inflammation. These findings suggest that the gut microbiota may contribute to RA development via interactions with the host immune system.

Keywords: Rheumatoid arthritis, gut microbiome, 16S rRNA gene sequencing, Inflammation, biomarker

Received: 06 May 2019; Accepted: 09 Oct 2019.

Copyright: © 2019 YANG, Chen, lin, Xu, He, Ji, Yanqin, Shen, Li, Liu, DONG, Tang, Pei, Yang, Lu, Xiao and GUO. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xiao-kui GUO, School of Medicine, Shanghai Jiao Tong University, Shanghai, Shanghai Municipality, China, xkguo@shsmu.edu.cn