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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Endocrinol. | doi: 10.3389/fendo.2019.00686

The effect of advanced maternal age on embryo morphokinetics

 Miriam Warshaviak1*, Yael Kalma1, Ariela Carmon1, Nivin Samara1,  Michal Dviri1, Foad Azem1 and  Dalit Ben-Yosef1, 2
  • 1Tel Aviv Sourasky Medical Center, Israel
  • 2Tel Aviv University, Israel

PURPOSE
To compare the morphokinetic parameters of preimplantation development between embryos of women of advanced maternal age (AMA) and young women.

METHODS
Time-lapse microscopy was used to compare morphokinetic variables between 495 embryos of AMA women ≥ age 42 years and 653 embryos of young patients (< age 38 years) who underwent IVF in our unit. Developmental events annotated and analyzed include observed cell divisions in correlation to the timing of fertilization, synchrony of the second (s2) and third cell cycles (s3) and the duration to the second (cc2) and third cleavages (cc3).

RESULTS
No significant differences were observed in cleavage times between the embryos of AMA and the control embryos. Interestingly, the older embryos appear to be more prone to developmental arrest (a higher percentage of embryos of older women arrested at 4-7 cells resulting in less embryos reaching the 8-cell stage (66% vs. 72% respectively;), though this difference did not reach a significance at least during the first three days of development (p>0.05).

CONCLUSIONS
While early morphokinetic parameters do not reflect dynamics unique to embryos of older women, a tendency towards developmental arrest was observed, which would likely be even more pronounced at later stages of development.

Keywords: Embryo development, Advanced maternal age, Time lapse microscopy, Morphokinetics, In Vitro Fertilisation (IVF)

Received: 27 May 2019; Accepted: 20 Sep 2019.

Copyright: © 2019 Warshaviak, Kalma, Carmon, Samara, Dviri, Azem and Ben-Yosef. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: MD. Miriam Warshaviak, Tel Aviv Sourasky Medical Center, Tel Aviv, 6997801, Tel Aviv, Israel, mwarshaviak@gmail.com