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ORIGINAL RESEARCH article

Front. Endocrinol.
Sec. Reproduction
Volume 15 - 2024 | doi: 10.3389/fendo.2024.1414289
This article is part of the Research Topic Weighing the Impact of Being Overweight on Female Reproductive Function and Fertility View all 3 articles

High coverage of targeted lipidomics revealed lipid changes in the follicular fluid of patients with insulin-resistant polycystic ovary syndrome and a positive correlation between plasmalogens and oocyte quality

Provisionally accepted
Meizi Zhang Meizi Zhang 1*Yuanyuan Wang Yuanyuan Wang 1*Jianyong Di Jianyong Di 1*Xuanlin Zhang Xuanlin Zhang 1Ye Liu Ye Liu 1*Yixin Zhang Yixin Zhang 1Bowen Li Bowen Li 2Simeng Qi Simeng Qi 2*Xiaomin Cao Xiaomin Cao 1*Li Liu Li Liu 1*Shouzeng Liu Shouzeng Liu 1*Fengqin Xu Fengqin Xu 1*
  • 1 Tianjin First Central Hospital, Tianjin, China
  • 2 Other, Changzhou, China

The final, formatted version of the article will be published soon.

    Background: Polycystic ovary syndrome with insulin resistance (PCOS-IR) is the most common endocrine and metabolic disease in women of reproductive age, and low fertility in PCOS patients may be associated with oocyte quality; however, the molecular mechanism through which PCOS-IR affects oocyte quality remains unknown.Methods: A total of 22 women with PCOS-IR and 23 women without polycystic ovary syndrome (control) who underwent in vitro fertilization and embryo transfer were recruited, and clinical information pertaining to oocyte quality was analyzed.Lipid components of follicular fluid (FF) were detected using high-coverage targeted 2 lipidomics, which identified 344 lipid species belonging to 19 lipid classes. The exact lipid species associated with oocyte quality were identified.The number (rate) of two pronuclear (2PN) zygotes, the number (rate) of 2PN cleaved embryos, and the number of high-quality embryos were significantly lower in the PCOS-IR group. A total of 19 individual lipid classes and 344 lipid species were identified and quantified. The concentrations of the 19 lipid species in the normal follicular fluid (control) ranged between 10 -3 mol/L and 10 -9 mol/L. In addition, 39 lipid species were significantly reduced in the PCOS-IR group, among which plasmalogens were positively correlated with oocyte quality.Conclusions: This study measured the levels of various lipids in follicular fluid, identified a significantly altered lipid profile in the FF of PCOS-IR patients, and established a correlation between poor oocyte quality and plasmalogens in PCOS-IR patients. These findings have contributed to the development of plasmalogen replacement therapy to enhance oocyte quality and have improved culture medium formulations for oocyte in vitro maturation (IVM).

    Keywords: Polycystic Ovary Syndrome, Follicular Fluid, lipidomics, Oocyte quality, Plasmalogens

    Received: 08 Apr 2024; Accepted: 16 May 2024.

    Copyright: © 2024 Zhang, Wang, Di, Zhang, Liu, Zhang, Li, Qi, Cao, Liu, Liu and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Meizi Zhang, Tianjin First Central Hospital, Tianjin, China
    Yuanyuan Wang, Tianjin First Central Hospital, Tianjin, China
    Jianyong Di, Tianjin First Central Hospital, Tianjin, China
    Ye Liu, Tianjin First Central Hospital, Tianjin, China
    Simeng Qi, Other, Changzhou, China
    Xiaomin Cao, Tianjin First Central Hospital, Tianjin, China
    Li Liu, Tianjin First Central Hospital, Tianjin, China
    Shouzeng Liu, Tianjin First Central Hospital, Tianjin, China
    Fengqin Xu, Tianjin First Central Hospital, Tianjin, China

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