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Front. Immunol. | doi: 10.3389/fimmu.2019.00160

The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells

  • 1Chonnam National University, South Korea
  • 2Universidad Miguel Hernández de Elche, Spain
  • 3Unidad de Proteomica, Centro Nacional de Biotecnología (CNB), Spain

Rock bream iridovirus (RBIV) causes severe mass mortality in Korean rock bream (Oplegnathus fasciatus) populations. To date, immune defense mechanisms of rock bream against RBIV are unclear. While red blood cells (RBCs) are known to be involved in the immune response against viral infections, the participation of rock bream RBCs in the immune response against RBIV has not been studied yet. In this study, we examined induction of the immune response in rock bream RBCs after RBIV infection. Each fish was injected with RBIV, and virus copy number in RBCs gradually increased from 4 days post-infection (dpi), peaking at 10 dpi. A total of 318 proteins were significantly regulated in RBCs from RBIV-infected individuals, 183 proteins were upregulated and 135 proteins were downregulated. Differentially upregulated proteins included those involved in cellular amino acid metabolic processes, cellular detoxification, snRNP assembly, and the spliceosome. Remarkably, the MHC class I-related protein pathway was upregulated during RBIV infection. Simultaneously, the regulation of apoptosis-related proteins, including caspase-6 (CASP6), caspase-9 (CASP9), Fas cell surface death receptor (FAS), desmoplakin (DSP), and p21 (RAC1)-activated kinase 2 (PAK2) changed with RBIV infection. Interestingly, the expression of genes within the ISG15 antiviral mechanism-related pathway, including filamin B (FLNB), interferon regulatory factor 3 (IRF3), nucleoporin 35 (NUP35), tripartite motif-containing 25 (TRIM25), and karyopherin subunit alpha 3 (KPNA3) were downregulated in RBCs from RBIV-infected individuals. Overall, these findings contribute to the understanding of RBIV pathogenesis and host interaction.

Keywords: Rock bream, RBIV, red blood cells, erythrocyte, Proteome, MHC class I, Apoptosis, ISG15

Received: 28 Jun 2018; Accepted: 17 Jan 2019.

Edited by:

Brian Dixon, University of Waterloo, Canada

Reviewed by:

Magdalena Chadzińska, Jagiellonian University, Poland
Stephanie DeWitte-Orr, Wilfrid Laurier University, Canada  

Copyright: © 2019 Jung, Gras, Ciordia, Mena, Jung and Ortega-Villaizan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Maria Del Mar Ortega-Villaizan, Universidad Miguel Hernández de Elche, Elche, 03202, Spain, mortega-villaizan@umh.es