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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2019.00288

Immunogenicity and protective efficacy of T-cell epitopes derived from potential Th1 stimulatory proteins of Leishmania (Leishmania) donovani

  • 1Council of Scientific and Industrial Research (CSIR), India
  • 2Institute of Medical Sciences, Banaras Hindu University, India

Development of a suitable vaccine against visceral leishmaniasis (VL), a fatal parasitic disease, is considered to be vital for maintaining the success of kala-azar control programs. The fact that Leishmania-infected individuals generate life-long immunity offers a viable proposition in this direction. Our prior studies demonstrated that T-helper1 (Th1) type of cellular response was generated by six potential recombinant proteins viz. elongation factor-2 (elF-2), enolase, aldolase, triose phosphate isomerase (TPI), protein disulfide isomerase (PDI) and p45, derived from a soluble antigenic fraction (89.9 to 97.1 kDa) of Leishmania (Leishmania) donovani promastigote, in treated Leishmania patients and hamsters and showed significant prophylactic potential against experimental VL. Moreover, since, it is well-known that our immune system, in general, triggers production of specific protective immunity in response to a small number of amino acids (peptide), this led to the identification of antigenic epitopes of the above-stated proteins utilizing immunoinformatics. Out of thirty-six, three peptides - P-10 (enolase), P-14 and P-15 (TPI) elicited common significant lymphoproliferative as well as Th1-biased cytokine responses both in golden hamsters and human subjects. Further, immunization with these peptides plus BCG offered 75% prophylactic efficacy with boosted cellular immune response in golden hamsters against Leishmania challenge which is indicative of their candidature as potential vaccine targets.

Keywords: Visceral leishmaniasis, protective response, hamsters, Human PBMCs, Peptides, T-cell epitopes, immunoinformatics, Th1 stimulatory proteins

Received: 24 Sep 2018; Accepted: 04 Feb 2019.

Edited by:

Rashika El Ridi, Cairo University, Egypt

Reviewed by:

Paula M. De Luca, Fundação Oswaldo Cruz (Fiocruz), Brazil
Vanete Thomaz Soccol, Universidade Federal do Paraná, Brazil  

Copyright: © 2019 Dube, Joshi, Yadav, Rawat, Kumar, Ali, Sahasrabuddhe, Siddiqi, Haq and Sundar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Anuradha Dube, Council of Scientific and Industrial Research (CSIR), New Delhi, National Capital Territory of Delhi, India, anuradha_dube@hotmail.com