Original Research ARTICLE
Shorter TCR β-chains are highly enriched during thymic selection and antigen-driven selection
- 1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, China
- 2College of Materials and Textile, Zhejiang Sci-Tech University, China
- 3First Affiliated Hospital, College of Medicine, Zhejiang University, China
The adaptive immune system uses several strategies to generate a repertoire of T cell receptors (TCR) with sufficient diversity to recognize the universe of potential pathogens. However, it remains unclear how differences in the T cell receptor (TCR) contribute to heterogeneity in T cell state. In this study, we used polychromatic flow cytometry to isolate highly pure CD4+/CD8+ naive and memory T cells, and applied deep sequencing to characterize corresponding TCR β-chain (TCRβ) complementary-determining region 3 (CDR3) repertoires. We find that shorter TCRβ CDR3s with fewer insertions were highly enriched during thymic selection. Antigen-experienced T cells (memory T cells) harbour shorter CDR3s versus naive T cells. Moreover, the public TCRβ CDR3 clonotypes within cell subsets or interindividual tend to have shorter CDR3 length and a significantly larger size compared with “private” clonotypes. Taken together, shorter CDR3s highly enriched during thymic selection and antigen-driven selection, and further enriched in public T-cell responses. These results indicated that it may be evolutionary pressures drive short CDR3s to recognize most of antigen in nature.
Keywords: T cell receptor, Cell subsets, Deep sequencing, memory T cell, naive T cell
Received: 31 Oct 2018;
Accepted: 05 Feb 2019.
Edited by:Remy Bosselut, National Cancer Institute (NCI), United States
Reviewed by:Linrong Lu, Zhejiang University, China
Thomas Ciucci, National Cancer Institute (NCI), United States
Copyright: © 2019 Hou, Zeng, Zhang, Chen, liang, Yang, Yang, Liu and Diao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Hongyan Diao, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, firstname.lastname@example.org